Restriction map for
pFN21AE4420
(Circular) (Six-base) MAPSORT of: pFN21AE4420.seq Check: 8733 from: 1 to: 6010
pFN21AE4420 6010 bp
With 182 enzymes: SgfI *
March 28, 2012 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2025 2025
Size: 6010
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3820 278
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3353 1937
Size: 1416 4594
Acc65I G'GTAC_C
Cuts at: 3331 3331
Size: 6010
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4073 5457 5725 1632
Size: 2441 1384 268 1917
Fragments arranged by size:
2441 1917 1384 268
AfeI AGC'GCT
Cuts at: 2453 2453
Size: 6010
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 4909 5886 1203
Size: 3706 977 1327
Fragments arranged by size:
3706 1327 977
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 4739 4739
Size: 6010
AleI CACnn'nnGTG
Cuts at: 1166 3286 1166
Size: 2120 3890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2762 1184
Size: 1578 4432
AlwNI CAG_nnn'CTG
Cuts at: 3753 5325 3753
Size: 1572 4438
ApaI G_GGCC'C
Cuts at: 2078 3042 3070 3195 2078
Size: 964 28 125 4893
Fragments arranged by size:
4893 964 125 28
ApaLI G'TGCA_C
Cuts at: 4067 5223 4067
Size: 1156 4854
ApoI r'AATT_y
Cuts at: 1493 3319 3545 3697 5806 1493
Size: 1826 226 152 2109 1697
Fragments arranged by size:
2109 1826 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 3335 1870
Size: 73 1392 4545
Fragments arranged by size:
4545 1392 73
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2130 2243 2403 2558 3209
Size: 325 217 627 343 113 160 155 651
Cuts at: 3209 3280 3331 4786 618
Size: 71 51 1455 1842
Fragments arranged by size:
1842 1455 651 627 343 325 217 160
155 113 71 51
BanII G_rGCy'C
Cuts at: 1391 2078 3042 3070 3195 3329 1391
Size: 687 964 28 125 134 4072
Fragments arranged by size:
4072 964 687 134 125 28
BbeI G_GCGC'C
Cuts at: 2407 2407
Size: 6010
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2161 928
Size: 971 262 4777
Fragments arranged by size:
4777 971 262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4201 4235 1903
Size: 34 2264 34 3678
Fragments arranged by size:
3678 2264 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3902 5112 3902
Size: 1210 4800
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6010
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 2552 2601
Size: 107 122 71 631 605 649 231 49
Cuts at: 2601 2673 2945 4621 136
Size: 72 272 1676 1525
Fragments arranged by size:
1676 1525 649 631 605 272 231 122
107 72 71 49
BglII A'GATC_T
Cuts at: 2868 6005 2868
Size: 3137 2873
BlpI GC'TnA_GC
Cuts at: 3427 3427
Size: 6010
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2246 2464 2581 2669 2686 2728 2768
Size: 288 168 218 117 88 17 42 40
Cuts at: 2768 3042 3070 3195 3214 4071 5227 1790
Size: 274 28 125 19 857 1156 2573
Fragments arranged by size:
2573 1156 857 288 274 218 168 125
117 88 42 40 28 19 17
BmgBI CAC'GTC
Cuts at: 1318 2155 1318
Size: 837 5173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2119 2277 2401
Size: 416 53 83 399 24 702 158 124
Cuts at: 2401 2434 3044 3741 4699 442
Size: 33 610 697 958 1753
Fragments arranged by size:
1753 958 702 697 610 416 399 158
124 83 53 33 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2978 2978
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2549 2780 2933 2973 4670 1239
Size: 917 393 231 153 40 1697 2579
Fragments arranged by size:
2579 1697 917 393 231 153 40
Bpu10I CC'TnA_GC
Cuts at: 3197 3197
Size: 6010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4135 5000 5298 4135
Size: 865 298 4847
Fragments arranged by size:
4847 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4673 882
Size: 617 3174 2219
Fragments arranged by size:
3174 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3801 493
Size: 711 2597 2702
Fragments arranged by size:
2702 2597 711
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2404 3817 4199 4834
Size: 53 83 186 936 871 1413 382 635
Cuts at: 4834 275
Size: 1451
Fragments arranged by size:
1451 1413 936 871 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4443 4841 5115 5262 5673 4443
Size: 398 274 147 411 4780
Fragments arranged by size:
4780 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 1178
Size: 30 165 30 861 30 4894
Fragments arranged by size:
4894 861 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2487 3131 3134 1186
Size: 1301 644 3 4062
Fragments arranged by size:
4062 1301 644 3
BseYI C'CCAG_C
Cuts at: 2235 2239 2412 2569 2735 2973 5213 5709 2235
Size: 4 173 157 166 238 2240 496 2536
Fragments arranged by size:
2536 2240 496 238 173 166 157 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2523 1163
Size: 573 787 4650
Fragments arranged by size:
4650 787 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3460 4221 5249 1540
Size: 275 186 1459 761 1028 2301
Fragments arranged by size:
2301 1459 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2919 2964 3095 4071 4156 5227 2919
Size: 45 131 976 85 1071 3702
Fragments arranged by size:
3702 1071 976 131 85 45
BsmI GAATG_Cn'
Cuts at: 2119 2305 2810 3521 3614 2119
Size: 186 505 711 93 4515
Fragments arranged by size:
4515 711 505 186 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2246 2464 2581 2669 2686 2728
Size: 399 288 168 218 117 88 17 42
Cuts at: 2728 2768 2919 2964 3042 3070 3095 3195 3214
Size: 40 151 45 78 28 25 100 19
Cuts at: 3214 3329 4071 4156 5227 1391
Size: 115 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 288 218 168 151
117 115 100 88 85 78 45 42
40 28 25 19 17
BspEI T'CCGG_A
Cuts at: 5673 5673
Size: 6010
BspHI T'CATG_A
Cuts at: 3899 5629 3899
Size: 1730 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2025 2365 3365 844
Size: 1181 340 1000 3489
Fragments arranged by size:
3489 1181 1000 340
BsrBI CCG'CTC
Cuts at: 1394 1823 3295 3897 1394
Size: 429 1472 602 3507
Fragments arranged by size:
3507 1472 602 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4505 4679 65
Size: 1183 3257 174 1396
Fragments arranged by size:
3257 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 2445 2657 2848 4654 4841 1700
Size: 110 281 354 212 191 1806 187 2869
Fragments arranged by size:
2869 1806 354 281 212 191 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6010
BssSI C'ACGA_G
Cuts at: 4070 5082 4070
Size: 1012 4998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6010
BstBI TT'CG_AA
Cuts at: 4814 4814
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1184 2834 1184
Size: 1650 4360
BstYI r'GATC_y
Cuts at: 1972 2868 3340 3707 4092 4109 5561 5647 5659
Size: 896 472 367 385 17 1452 86 12
Cuts at: 5659 6005 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 896 472 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2499 2499
Size: 6010
BtgI C'CryG_G
Cuts at: 513 1065 2004 2203 3100 3284 513
Size: 552 939 199 897 184 3239
Fragments arranged by size:
3239 939 897 552 199 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 2579 2760 3526 4320 4340 2579
Size: 181 766 794 20 4249
Fragments arranged by size:
4249 794 766 181 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 3314 3314
Size: 6010
DraIII CAC_nnn'GTG
Cuts at: 2127 2149 2800 2127
Size: 22 651 5337
Fragments arranged by size:
5337 651 22
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2159 5017 809
Size: 507 843 2858 1802
Fragments arranged by size:
2858 1802 843 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2489 2659 2802
Size: 54 1425 50 275 18 659 170 143
Cuts at: 2802 3097 3103 3457 4346 5975 8
Size: 295 6 354 889 1629 43
Fragments arranged by size:
1629 1425 889 659 354 295 275 170
143 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3457 1537
Size: 275 1645 4090
Fragments arranged by size:
4090 1645 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 3293 3940 1353
Size: 689 1251 647 3423
Fragments arranged by size:
3423 1251 689 647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3104 4597 4971 5117 387
Size: 1064 138 1515 1493 374 146 1280
Fragments arranged by size:
1515 1493 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2549 2780 2933 2973 4073 4670
Size: 393 524 393 231 153 40 1100 597
Cuts at: 4670 5457 5725 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1100 787 597 524 393 393 268
231 153 40
EcoO109I rG'GnC_Cy
Cuts at: 2074 2075 3038 3039 3066 3067 3192 2074
Size: 1 963 1 27 1 125 4892
Fragments arranged by size:
4892 963 125 27 1 1 1
EcoRI G'AATT_C
Cuts at: 3319 3697 3319
Size: 378 5632
FspI TGC'GCA
Cuts at: 4516 4516
Size: 6010
HaeII r_GCGC'y
Cuts at: 1678 2407 2455 5157 1678
Size: 729 48 2702 2531
Fragments arranged by size:
2702 2531 729 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4651 4683 4725 4757 1373
Size: 32 135 32 3079 32 42 32 2626
Fragments arranged by size:
3079 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3354 3600 669
Size: 988 281 1416 246 3079
Fragments arranged by size:
3079 1416 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6010
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KasI G'GCGC_C
Cuts at: 2403 2403
Size: 6010
KpnI G_GTAC'C
Cuts at: 3335 3335
Size: 6010
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5124 5308 1305
Size: 3819 184 2007
Fragments arranged by size:
3819 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2491 10
Size: 54 1425 343 659 3529
Fragments arranged by size:
3529 1425 659 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 3286 3968 4327 4486 518
Size: 648 1578 542 682 359 159 2042
Fragments arranged by size:
2042 1578 682 648 542 359 159
MspA1I CmG'CkG
Cuts at: 1606 2205 2239 2569 2951 3108 3426 4103 5251
Size: 599 34 330 382 157 318 677 1148
Cuts at: 5251 5496 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 599 382 330 318 245
157 34
NarI GG'CG_CC
Cuts at: 2404 2404
Size: 6010
NcoI C'CATG_G
Cuts at: 513 1065 2004 3100 513
Size: 552 939 1096 3423
Fragments arranged by size:
3423 1096 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 2749 3307 3368 4913 1132
Size: 97 1520 558 61 1545 2229
Fragments arranged by size:
2229 1545 1520 558 97 61
PasI CC'CwG_GG
Cuts at: 1348 2189 2941 1348
Size: 841 752 4417
Fragments arranged by size:
4417 841 752
PciI A'CATG_T
Cuts at: 4909 4909
Size: 6010
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2109 1108
Size: 1001 5009
PfoI T'CCnGG_A
Cuts at: 2334 2334
Size: 6010
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PspOMI G'GGCC_C
Cuts at: 2074 3038 3066 3191 2074
Size: 964 28 125 4893
Fragments arranged by size:
4893 964 125 28
PstI C_TGCA'G
Cuts at: 830 3080 3362 830
Size: 2250 282 3478
Fragments arranged by size:
3478 2250 282
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 2239 2569 2951 2239
Size: 330 382 5298
Fragments arranged by size:
5298 382 330
SacII CC_GC'GG
Cuts at: 2206 2206
Size: 6010
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SapI GCTCTTCn'nnn_
Cuts at: 3293 3293
Size: 6010
SbfI CC_TGCA'GG
Cuts at: 3362 3362
Size: 6010
ScaI AGT'ACT
Cuts at: 4258 5970 4258
Size: 1712 4298
SfcI C'TryA_G
Cuts at: 826 1045 2227 3076 3358 4493 5174 5365 826
Size: 219 1182 849 282 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 849 681 282 219 191
SfoI GGC'GCC
Cuts at: 2405 2405
Size: 6010
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 3337 1872
Size: 1465 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4114 5015 5277 820
Size: 197 926 2171 901 262 1553
Fragments arranged by size:
2171 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SspI AAT'ATT
Cuts at: 5 52 3934 5
Size: 47 3882 2081
Fragments arranged by size:
3882 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6010
StyI C'CwwG_G
Cuts at: 513 1065 2004 2062 2433 2991 3100 3449 513
Size: 552 939 58 371 558 109 349 3074
Fragments arranged by size:
3074 939 558 552 371 349 109 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 2956 4037 4054 4207 4392 4837
Size: 1255 558 818 1081 17 153 185 445
Cuts at: 4837 325
Size: 1498
Fragments arranged by size:
1498 1255 1081 818 558 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4256 5968 96
Size: 274 80 33 51 1443 2279 1712 138
Fragments arranged by size:
2279 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5970 6002 5970
Size: 32 5978
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6010
XbaI T'CTAG_A
Cuts at: 3346 3346
Size: 6010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2948 3179 2948
Size: 231 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 3335 1870
Size: 1465 4545
XmnI GAAnn'nnTTC
Cuts at: 4139 4139
Size: 6010
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3818 276
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI