Restriction map for
pFN21AE4416
(Circular) (Six-base) MAPSORT of: pFN21AE4416.seq Check: 8485 from: 1 to: 5992
pFN21AE4416 5992 bp
With 182 enzymes: SgfI *
March 28, 2012 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 1937
Size: 1398 4594
Acc65I G'GTAC_C
Cuts at: 2988 3313 2988
Size: 325 5667
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2306 2411 2921 3233 4055 5439 5707 1632
Size: 674 105 510 312 822 1384 268 1917
Fragments arranged by size:
1917 1384 822 674 510 312 268 105
AfeI AGC'GCT
Cuts at: 2150 2339 2150
Size: 189 5803
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 2562 4891 5868 1203
Size: 1359 2329 977 1327
Fragments arranged by size:
2329 1359 1327 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 4721 4721
Size: 5992
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3260 1184
Size: 2076 3916
AlwNI CAG_nnn'CTG
Cuts at: 3239 3735 5307 3239
Size: 496 1572 3924
Fragments arranged by size:
3924 1572 496
ApaLI G'TGCA_C
Cuts at: 4049 5205 4049
Size: 1156 4836
ApoI r'AATT_y
Cuts at: 1493 2507 2539 2686 3301 3527 3679 5788 1493
Size: 1014 32 147 615 226 152 2109 1697
Fragments arranged by size:
2109 1697 1014 615 226 152 147 32
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 3317 1870
Size: 73 1374 4545
Fragments arranged by size:
4545 1374 73
BamHI G'GATC_C
Cuts at: 3322 3689 3322
Size: 367 5625
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2988 3313 4768 618
Size: 325 217 627 1201 325 1455 1842
Fragments arranged by size:
1842 1455 1201 627 325 325 217
BanII G_rGCy'C
Cuts at: 1391 2229 3311 1391
Size: 838 1082 4072
Fragments arranged by size:
4072 1082 838
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5021
BbvCI CC'TCA_GC
Cuts at: 2012 2334 2828 2012
Size: 322 494 5176
Fragments arranged by size:
5176 494 322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2450 2484 2652 2686 4183
Size: 34 50 34 429 34 168 34 1497
Cuts at: 4183 4217 1903
Size: 34 3678
Fragments arranged by size:
3678 1497 429 168 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2447 3884 5094 2447
Size: 1437 1210 3345
Fragments arranged by size:
3345 1437 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2864 4603 136
Size: 107 122 71 631 605 1192 1739 1525
Fragments arranged by size:
1739 1525 1192 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2497 5987 2497
Size: 3490 2502
BlpI GC'TnA_GC
Cuts at: 3043 3409 3043
Size: 366 5626
Bme1580I G_kGCm'C
Cuts at: 1790 4053 5209 1790
Size: 2263 1156 2573
Fragments arranged by size:
2573 2263 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5992
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2152 2497 3723
Size: 416 53 83 399 24 735 345 1226
Cuts at: 3723 4681 442
Size: 958 1753
Fragments arranged by size:
1753 1226 958 735 416 399 345 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2366 1056
Size: 1310 4682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4652 1239
Size: 3413 2579
Bpu10I CC'TnA_GC
Cuts at: 2012 2334 2828 2012
Size: 322 494 5176
Fragments arranged by size:
5176 494 322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2904 4117 4982 5280 2904
Size: 1213 865 298 3616
Fragments arranged by size:
3616 1213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2239 4655 882
Size: 617 740 2416 2219
Fragments arranged by size:
2416 2219 740 617
BsaAI yAC'GTr
Cuts at: 493 1204 3783 493
Size: 711 2579 2702
Fragments arranged by size:
2702 2579 711
BsaBI GATnn'nnATC
Cuts at: 5986 5986
Size: 5992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2120 3799 4181 4816
Size: 53 83 186 936 587 1679 382 635
Cuts at: 4816 275
Size: 1451
Fragments arranged by size:
1679 1451 936 635 587 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4425 4823 5097 5244 5655 4425
Size: 398 274 147 411 4762
Fragments arranged by size:
4762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3150 3180 1178
Size: 30 165 30 1747 30 3990
Fragments arranged by size:
3990 1747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3155 1186
Size: 1969 4023
BseYI C'CCAG_C
Cuts at: 5195 5691 5195
Size: 496 5496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 1163
Size: 573 387 5032
Fragments arranged by size:
5032 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3442 4203 5231 1540
Size: 275 186 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2098 2229 4053 4138 5209 2098
Size: 131 1824 85 1071 2881
Fragments arranged by size:
2881 1824 1071 131 85
BsmI GAATG_Cn'
Cuts at: 3503 3596 3503
Size: 93 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2229 3311 4053 4138 5209 1391
Size: 399 308 131 1082 742 85 1071 2174
Fragments arranged by size:
2174 1082 1071 742 399 308 131 85
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 3881 5611 3881
Size: 1730 4262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2246 2435 3347 844
Size: 1402 189 912 3489
Fragments arranged by size:
3489 1402 912 189
BsrBI CCG'CTC
Cuts at: 1394 1823 3879 1394
Size: 429 2056 3507
Fragments arranged by size:
3507 2056 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4487 4661 65
Size: 1183 3239 174 1396
Fragments arranged by size:
3239 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3069 4636 4823 1700
Size: 110 1259 1567 187 2869
Fragments arranged by size:
2869 1567 1259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5992
BssHII G'CGCG_C
Cuts at: 1925 2040 1925
Size: 115 5877
BssSI C'ACGA_G
Cuts at: 4052 5064 4052
Size: 1012 4980
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5992
BstBI TT'CG_AA
Cuts at: 4796 4796
Size: 5992
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5992
BstXI CCAn_nnnn'nTGG
Cuts at: 2787 2787
Size: 5992
BstYI r'GATC_y
Cuts at: 1972 2497 3322 3689 4074 4091 5543 5629 5641
Size: 525 825 367 385 17 1452 86 12
Cuts at: 5641 5987 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 825 525 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2127 513
Size: 552 1062 4378
Fragments arranged by size:
4378 1062 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3088 487
Size: 683 56 1862 3391
Fragments arranged by size:
3391 1862 683 56
BtsI GCAGTG_nn'
Cuts at: 3508 4302 4322 3508
Size: 794 20 5178
Fragments arranged by size:
5178 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5957
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4999 809
Size: 507 3683 1802
Fragments arranged by size:
3683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3439 4328 5957
Size: 54 1425 50 275 18 1609 889 1629
Cuts at: 5957 8
Size: 43
Fragments arranged by size:
1629 1609 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3439 1537
Size: 275 1627 4090
Fragments arranged by size:
4090 1627 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2213 3922 1353
Size: 819 41 1709 3423
Fragments arranged by size:
3423 1709 819 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4579 4953 5099 387
Size: 1064 138 2990 374 146 1280
Fragments arranged by size:
2990 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2227 2227
Size: 5992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2411 2921 3233 4055 4652 5439
Size: 393 674 105 510 312 822 597 787
Cuts at: 5439 5707 1239
Size: 268 1524
Fragments arranged by size:
1524 822 787 674 597 510 393 312
268 105
EcoNI CCTnn'n_nnAGG
Cuts at: 3036 3036
Size: 5992
EcoO109I rG'GnC_Cy
Cuts at: 2968 2968
Size: 5992
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2336 2746 2951 3305 2336
Size: 410 205 354 5023
Fragments arranged by size:
5023 410 354 205
FspI TGC'GCA
Cuts at: 2431 4498 2431
Size: 2067 3925
HaeII r_GCGC'y
Cuts at: 1678 2152 2341 5139 1678
Size: 474 189 2798 2531
Fragments arranged by size:
2798 2531 474 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2726 2758 4633 4665 4707
Size: 32 135 32 1154 32 1875 32 42
Cuts at: 4707 4739 1373
Size: 32 2626
Fragments arranged by size:
2626 1875 1154 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3336 3582 669
Size: 988 281 1398 246 3079
Fragments arranged by size:
3079 1398 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KpnI G_GTAC'C
Cuts at: 2992 3317 2992
Size: 325 5667
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2267 2395 2686 2768 2929 5106 5290 1305
Size: 962 128 291 82 161 2177 184 2007
Fragments arranged by size:
2177 2007 962 291 184 161 128 82
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4170
Fragments arranged by size:
4170 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 3950 4309 4468 518
Size: 648 1050 1734 359 159 2042
Fragments arranged by size:
2042 1734 1050 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2832 3408 4085 5233 5478 1606
Size: 405 821 576 677 1148 245 2120
Fragments arranged by size:
2120 1148 821 677 576 405 245
NcoI C'CATG_G
Cuts at: 513 1065 2127 513
Size: 552 1062 4378
Fragments arranged by size:
4378 1062 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NheI G'CTAG_C
Cuts at: 1052 2362 1052
Size: 1310 4682
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 2566 3350 4895 1132
Size: 97 1337 784 1545 2229
Fragments arranged by size:
2229 1545 1337 784 97
PasI CC'CwG_GG
Cuts at: 1348 2084 2441 3118 1348
Size: 736 357 677 4222
Fragments arranged by size:
4222 736 677 357
PciI A'CATG_T
Cuts at: 2562 4891 2562
Size: 2329 3663
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2133 1108
Size: 1025 4967
PfoI T'CCnGG_A
Cuts at: 2708 2708
Size: 5992
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3018 3050 4061 4093 5574 5606 3018
Size: 32 1011 32 1481 32 3404
Fragments arranged by size:
3404 1481 1011 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2968 2968
Size: 5992
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5992
PsiI TTA'TAA
Cuts at: 2556 3562 2556
Size: 1006 4986
PstI C_TGCA'G
Cuts at: 830 3344 830
Size: 2514 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
PvuII CAG'CTG
Cuts at: 2011 2832 2011
Size: 821 5171
SacI G_AGCT'C
Cuts at: 2229 2229
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SapI GCTCTTCn'nnn_
Cuts at: 2172 2213 2172
Size: 41 5951
SbfI CC_TGCA'GG
Cuts at: 3344 3344
Size: 5992
ScaI AGT'ACT
Cuts at: 2669 4240 5952 2669
Size: 1571 1712 2709
Fragments arranged by size:
2709 1712 1571
SfcI C'TryA_G
Cuts at: 826 1045 3054 3340 4475 5156 5347 826
Size: 219 2009 286 1135 681 191 1471
Fragments arranged by size:
2009 1471 1135 681 286 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 3319 1872
Size: 1447 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2919 4096 4997 5259 820
Size: 197 926 976 1177 901 262 1553
Fragments arranged by size:
1553 1177 976 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 3350 1132
Size: 2218 3774
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5992
StyI C'CwwG_G
Cuts at: 513 1065 2127 2568 2973 3431 513
Size: 552 1062 441 405 458 3074
Fragments arranged by size:
3074 1062 552 458 441 405
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4019 4036 4189 4374 4819 325
Size: 1255 2439 17 153 185 445 1498
Fragments arranged by size:
2439 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2601 2667 4238
Size: 274 80 33 51 1443 624 66 1571
Cuts at: 4238 5950 96
Size: 1712 138
Fragments arranged by size:
1712 1571 1443 624 274 138 80 66
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3150 3182 5952 5984 3150
Size: 32 2770 32 3158
Fragments arranged by size:
3158 2770 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5992
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 3317 1870
Size: 1447 4545
XmnI GAAnn'nnTTC
Cuts at: 2207 4121 2207
Size: 1914 4078
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI