Restriction map for
pFN21AE4410
(Circular) (Six-base) MAPSORT of: pFN21AE4410.seq Check: 9418 from: 1 to: 5974
pFN21AE4410 5974 bp
With 182 enzymes: SgfI *
March 28, 2012 13:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2922 4037 5421 5689 1632
Size: 1290 1115 1384 268 1917
Fragments arranged by size:
1917 1384 1290 1115 268
AfeI AGC'GCT
Cuts at: 2164 3143 2164
Size: 979 4995
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 3717 5289 3717
Size: 1572 4402
ApaI G_GGCC'C
Cuts at: 3156 3156
Size: 5974
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2378 2829 3283 3509 3661 5770 1493
Size: 885 451 454 226 152 2109 1697
Fragments arranged by size:
2109 1697 885 454 451 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2278 3299 1870
Size: 73 335 1021 4545
Fragments arranged by size:
4545 1021 335 73
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 4750 618
Size: 325 217 627 1508 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2040 3156 3293 1391
Size: 649 1116 137 4072
Fragments arranged by size:
4072 1116 649 137
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3067 928
Size: 971 1168 3835
Fragments arranged by size:
3835 1168 971
BbvCI CC'TCA_GC
Cuts at: 2328 2328
Size: 5974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2750 4585 136
Size: 107 122 71 631 605 1078 1835 1525
Fragments arranged by size:
1835 1525 1078 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 2444 3391 2444
Size: 947 5027
Bme1580I G_kGCm'C
Cuts at: 1790 3156 4035 5191 1790
Size: 1366 879 1156 2573
Fragments arranged by size:
2573 1366 1156 879
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3217 3705 4663
Size: 416 53 83 399 24 1800 488 958
Cuts at: 4663 442
Size: 1753
Fragments arranged by size:
1800 1753 958 488 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3160 3160
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4634 1239
Size: 3395 2579
Bpu10I CC'TnA_GC
Cuts at: 2328 2328
Size: 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2820 4099 4964 5262 2820
Size: 1279 865 298 3532
Fragments arranged by size:
3532 1279 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2717 4637 882
Size: 617 1218 1920 2219
Fragments arranged by size:
2219 1920 1218 617
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3152 1186
Size: 1966 4008
BseYI C'CCAG_C
Cuts at: 5177 5673 5177
Size: 496 5478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2823 4035 4120 5191 2823
Size: 1212 85 1071 3606
Fragments arranged by size:
3606 1212 1071 85
BsmI GAATG_Cn'
Cuts at: 2843 3485 3578 2843
Size: 642 93 5239
Fragments arranged by size:
5239 642 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2935 2935
Size: 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2823 3156 3293 4035 4120 5191
Size: 399 250 783 333 137 742 85 1071
Cuts at: 5191 1391
Size: 2174
Fragments arranged by size:
2174 1071 783 742 399 333 250 137
85
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2887 3329 844
Size: 2043 442 3489
Fragments arranged by size:
3489 2043 442
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2577 3274 4469 4643 65
Size: 1183 1329 697 1195 174 1396
Fragments arranged by size:
1396 1329 1195 1183 697 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstXI CCAn_nnnn'nTGG
Cuts at: 3248 3248
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2205 3304 3671 4056 4073 5525 5611 5623
Size: 233 1099 367 385 17 1452 86 12
Cuts at: 5623 5969 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1099 385 367 346 233 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5422
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 3490 4284 4304 3490
Size: 794 20 5160
Fragments arranged by size:
5160 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 2646 3278 2646
Size: 632 5342
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939
Size: 54 1425 50 275 18 1591 889 1629
Cuts at: 5939 8
Size: 43
Fragments arranged by size:
1629 1591 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2597 2926 3904 1353
Size: 1244 329 978 3423
Fragments arranged by size:
3423 1244 978 329
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2096 4561 4935 5081 387
Size: 1064 138 507 2465 374 146 1280
Fragments arranged by size:
2465 1280 1064 507 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2922 4037 4634 5421 5689 1239
Size: 393 1290 1115 597 787 268 1524
Fragments arranged by size:
1524 1290 1115 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2752 3152 3153 3175 2752
Size: 400 1 22 5551
Fragments arranged by size:
5551 400 22 1
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 5974
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 2166 3145 5121 1678
Size: 488 979 1976 2531
Fragments arranged by size:
2531 1976 979 488
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 2981 3013 4615
Size: 32 135 32 689 32 688 32 1602
Cuts at: 4615 4647 4689 4721 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1602 689 688 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 3318 3564 669
Size: 988 281 568 812 246 3079
Fragments arranged by size:
3079 988 812 568 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2176 2257 5088 5272 1305
Size: 871 81 2831 184 2007
Fragments arranged by size:
2831 2007 871 184 81
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3932 4291 4450 518
Size: 648 2766 359 159 2042
Fragments arranged by size:
2766 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2960 3390 4067 5215 5460 1606
Size: 417 937 430 677 1148 245 2120
Fragments arranged by size:
2120 1148 937 677 430 417 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5422
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 3332 4877 1132
Size: 97 2103 1545 2229
Fragments arranged by size:
2229 2103 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 2778 3544 2778
Size: 766 5208
PspOMI G'GGCC_C
Cuts at: 3152 3152
Size: 5974
PstI C_TGCA'G
Cuts at: 830 2866 3326 830
Size: 2036 460 3478
Fragments arranged by size:
3478 2036 460
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2023 2023
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 2862 3322 4457 5138 5329 826
Size: 219 1817 460 1135 681 191 1471
Fragments arranged by size:
1817 1471 1135 681 460 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2835 4078 4979 5241 820
Size: 197 926 892 1243 901 262 1553
Fragments arranged by size:
1553 1243 926 901 892 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 3241 3413 513
Size: 552 2176 172 3074
Fragments arranged by size:
3074 2176 552 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4001 4018 4171 4356 4801 325
Size: 1255 2421 17 153 185 445 1498
Fragments arranged by size:
2421 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4220 5932 96
Size: 274 80 33 51 1443 2243 1712 138
Fragments arranged by size:
2243 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5934 5966 5934
Size: 32 5942
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 2685 4103 2685
Size: 1418 4556
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI