Restriction map for
pFN21AE4409
(Circular) (Six-base) MAPSORT of: pFN21AE4409.seq Check: 8138 from: 1 to: 5974
pFN21AE4409 5974 bp
With 182 enzymes: SgfI *
March 28, 2012 13:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3098 3098
Size: 5974
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 2653 3295 2653
Size: 642 5332
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 2708 4037 5421 5689 1632
Size: 965 111 1329 1384 268 1917
Fragments arranged by size:
1917 1384 1329 965 268 111
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 3025 1166
Size: 1859 4115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 2179 2191 2686 2741 3208 3717 5289 2179
Size: 12 495 55 467 509 1572 2864
Fragments arranged by size:
2864 1572 509 495 467 55 12
ApaI G_GGCC'C
Cuts at: 3160 3215 3160
Size: 55 5919
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 3283 3509 3661 5770 1493
Size: 1790 226 152 2109 1697
Fragments arranged by size:
2109 1790 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2616 3299 1870
Size: 73 673 683 4545
Fragments arranged by size:
4545 683 673 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2637 2670 2637
Size: 33 5941
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2097 2653 2801 3295 4750
Size: 325 217 627 310 556 148 494 1455
Cuts at: 4750 618
Size: 1842
Fragments arranged by size:
1842 1455 627 556 494 325 310 217
148
BanII G_rGCy'C
Cuts at: 1391 2633 3160 3171 3215 3293 1391
Size: 1242 527 11 44 78 4072
Fragments arranged by size:
4072 1242 527 78 44 11
BbeI G_GCGC'C
Cuts at: 2101 2101
Size: 5974
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2248 2562 928
Size: 971 349 314 4340
Fragments arranged by size:
4340 971 349 314
BbvCI CC'TCA_GC
Cuts at: 3222 3222
Size: 5974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2883 2917 4165 4199 1903
Size: 34 946 34 1248 34 3678
Fragments arranged by size:
3678 1248 946 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 2815 1548
Size: 1267 4707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2831 4585 136
Size: 107 122 71 631 605 1159 1754 1525
Fragments arranged by size:
1754 1525 1159 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3165 3391 3165
Size: 226 5748
Bme1580I G_kGCm'C
Cuts at: 1790 3160 3215 4035 5191 1790
Size: 1370 55 820 1156 2573
Fragments arranged by size:
2573 1370 1156 820 55
BmgBI CAC'GTC
Cuts at: 1318 2518 1318
Size: 1200 4774
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2479 3078 3705
Size: 416 53 83 399 24 1062 599 627
Cuts at: 3705 4663 442
Size: 958 1753
Fragments arranged by size:
1753 1062 958 627 599 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2988 1056
Size: 1932 4042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 3063 3155 4634 1239
Size: 926 898 92 1479 2579
Fragments arranged by size:
2579 1479 926 898 92
Bpu10I CC'TnA_GC
Cuts at: 2958 3222 2958
Size: 264 5710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2089 4099 4964 5262 2089
Size: 2010 865 298 2801
Fragments arranged by size:
2801 2010 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2529 4637 882
Size: 617 1030 2108 2219
Fragments arranged by size:
2219 2108 1030 617
BsaAI yAC'GTr
Cuts at: 493 1204 2722 3130 3765 493
Size: 711 1518 408 635 2702
Fragments arranged by size:
2702 1518 711 635 408
BsaBI GATnn'nnATC
Cuts at: 2604 5968 2604
Size: 3364 2610
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2098 3781 4163 4798
Size: 53 83 186 936 565 1683 382 635
Cuts at: 4798 275
Size: 1451
Fragments arranged by size:
1683 1451 936 635 565 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2223 4407 4805 5079 5226 5637 2223
Size: 2184 398 274 147 411 2560
Fragments arranged by size:
2560 2184 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2138 2168 1178
Size: 30 165 30 735 30 4984
Fragments arranged by size:
4984 735 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 3060 1186
Size: 976 898 4100
Fragments arranged by size:
4100 976 898
BseYI C'CCAG_C
Cuts at: 2052 2206 2286 2873 2941 5177 5673 2052
Size: 154 80 587 68 2236 496 2353
Fragments arranged by size:
2353 2236 587 496 154 80 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2196 1163
Size: 573 460 4941
Fragments arranged by size:
4941 573 460
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2089 3424 4185 5213 1540
Size: 275 186 88 1335 761 1028 2301
Fragments arranged by size:
2301 1335 1028 761 275 186 88
BsiHKAI G_wGCw'C
Cuts at: 2373 2394 3171 4035 4120 5191 2373
Size: 21 777 864 85 1071 3156
Fragments arranged by size:
3156 1071 864 777 85 21
BsmI GAATG_Cn'
Cuts at: 2058 3113 3485 3578 2058
Size: 1055 372 93 4454
Fragments arranged by size:
4454 1055 372 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2373 2394 2633 3160 3171 3215 3293
Size: 399 583 21 239 527 11 44 78
Cuts at: 3293 4035 4120 5191 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 583 527 399 239 85
78 44 21 11
BspEI T'CCGG_A
Cuts at: 2223 5637 2223
Size: 3414 2560
BspHI T'CATG_A
Cuts at: 2596 2818 3863 5593 2596
Size: 222 1045 1730 2977
Fragments arranged by size:
2977 1730 1045 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3098 3329 844
Size: 2254 231 3489
Fragments arranged by size:
3489 2254 231
BsrBI CCG'CTC
Cuts at: 1394 1823 2229 3861 1394
Size: 429 406 1632 3507
Fragments arranged by size:
3507 1632 429 406
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstXI CCAn_nnnn'nTGG
Cuts at: 2381 2381
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2034 3205 3304 3671 4056 4073 5525 5611
Size: 62 1171 99 367 385 17 1452 86
Cuts at: 5611 5623 5969 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1171 385 367 346 99 86
62 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2884 513
Size: 552 1819 3603
Fragments arranged by size:
3603 1819 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2248 3020 3490 4284 4304 2248
Size: 772 470 794 20 3918
Fragments arranged by size:
3918 794 772 470 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DraIII CAC_nnn'GTG
Cuts at: 2459 2459
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939
Size: 54 1425 50 275 18 1591 889 1629
Cuts at: 5939 8
Size: 43
Fragments arranged by size:
1629 1591 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3128 3904 1353
Size: 1775 776 3423
Fragments arranged by size:
3423 1775 776
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3169 3169
Size: 5974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2597 2708 3063 3155 4037 4634
Size: 393 533 432 111 355 92 882 597
Cuts at: 4634 5421 5689 1239
Size: 787 268 1524
Fragments arranged by size:
1524 882 787 597 533 432 393 355
268 111 92
EcoNI CCTnn'n_nnAGG
Cuts at: 2576 2576
Size: 5974
EcoO109I rG'GnC_Cy
Cuts at: 3156 3156
Size: 5974
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 5974
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 2101 5121 1678
Size: 423 3020 2531
Fragments arranged by size:
3020 2531 423
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2019 2051 2821 2853 4615
Size: 32 135 32 447 32 770 32 1762
Cuts at: 4615 4647 4689 4721 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1762 770 447 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2469 748
Size: 1721 4253
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KasI G'GCGC_C
Cuts at: 2097 2097
Size: 5974
KpnI G_GTAC'C
Cuts at: 2657 3299 2657
Size: 642 5332
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2331 2379 2415 3025 3932 4291 4450
Size: 648 1165 48 36 610 907 359 159
Cuts at: 4450 518
Size: 2042
Fragments arranged by size:
2042 1165 907 648 610 359 159 48
36
MspA1I CmG'CkG
Cuts at: 1606 2210 3390 4067 5215 5460 1606
Size: 604 1180 677 1148 245 2120
Fragments arranged by size:
2120 1180 1148 677 604 245
NarI GG'CG_CC
Cuts at: 2098 2098
Size: 5974
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5422
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 2984 1052
Size: 1932 4042
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 2548 3179 3332 4877 1132
Size: 97 1319 631 153 1545 2229
Fragments arranged by size:
2229 1545 1319 631 153 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3208 1108
Size: 2100 3874
PfoI T'CCnGG_A
Cuts at: 2274 2274
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 4043 4075 5556 5588 2861
Size: 32 1150 32 1481 32 3247
Fragments arranged by size:
3247 1481 1150 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspOMI G'GGCC_C
Cuts at: 3156 3211 3156
Size: 55 5919
PstI C_TGCA'G
Cuts at: 830 3326 830
Size: 2496 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2210 2210
Size: 5974
SacI G_AGCT'C
Cuts at: 3171 3171
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 2778 3322 4457 5138 5329 826
Size: 219 1733 544 1135 681 191 1471
Fragments arranged by size:
1733 1471 1135 681 544 219 191
SfoI GGC'GCC
Cuts at: 2099 2099
Size: 5974
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2068 4078 4979 5241 820
Size: 197 926 125 2010 901 262 1553
Fragments arranged by size:
2010 1553 926 901 262 197 125
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 2930 1517
Size: 1413 4561
StyI C'CwwG_G
Cuts at: 513 1065 3413 513
Size: 552 2348 3074
Fragments arranged by size:
3074 2348 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2565 4001 4018 4171 4356 4801 325
Size: 1255 985 1436 17 153 185 445 1498
Fragments arranged by size:
1498 1436 1255 985 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4220 5932 96
Size: 274 80 33 51 1443 2243 1712 138
Fragments arranged by size:
2243 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2138 2170 2205 2237 2486 2518 3070 3102 5934
Size: 32 35 32 249 32 552 32 2832
Cuts at: 5934 5966 2138
Size: 32 2146
Fragments arranged by size:
2832 2146 552 249 35 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3091 3091
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI