Restriction map for
pFN21AE4406
(Circular) (Six-base) MAPSORT of: pFN21AE4406.seq Check: 8155 from: 1 to: 5968
pFN21AE4406 5968 bp
With 182 enzymes: SgfI *
March 28, 2012 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3778 278
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3311 1937
Size: 1374 4594
Acc65I G'GTAC_C
Cuts at: 3289 3289
Size: 5968
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4031 5415 5683 1632
Size: 2399 1384 268 1917
Fragments arranged by size:
2399 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 4867 5844 1203
Size: 3664 977 1327
Fragments arranged by size:
3664 1327 977
AgeI A'CCGG_T
Cuts at: 4799 4799
Size: 5968
AhdI GACnn_n'nnGTC
Cuts at: 4697 4697
Size: 5968
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5968
AlwNI CAG_nnn'CTG
Cuts at: 2195 2492 2504 3711 5283 2195
Size: 297 12 1207 1572 2880
Fragments arranged by size:
2880 1572 1207 297 12
ApaLI G'TGCA_C
Cuts at: 4025 5181 4025
Size: 1156 4812
ApoI r'AATT_y
Cuts at: 1493 2144 2165 2380 2694 3219 3277 3503 3655
Size: 651 21 215 314 525 58 226 152
Cuts at: 3655 5764 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 651 525 314 226 215 152
58 21
AseI AT'TA_AT
Cuts at: 160 1032 2559 4522 160
Size: 872 1527 1963 1606
Fragments arranged by size:
1963 1606 1527 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 3293 1870
Size: 73 1350 4545
Fragments arranged by size:
4545 1350 73
BamHI G'GATC_C
Cuts at: 3298 3665 3298
Size: 367 5601
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3289 4744 618
Size: 325 217 627 1502 1455 1842
Fragments arranged by size:
1842 1502 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3287 1391
Size: 1896 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2749 928
Size: 971 850 4147
Fragments arranged by size:
4147 971 850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4159 4193 1903
Size: 34 2222 34 3678
Fragments arranged by size:
3678 2222 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3860 5070 3860
Size: 1210 4758
BclI T'GATC_A
Cuts at: 1548 2419 1548
Size: 871 5097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4579 136
Size: 107 122 71 631 605 2907 1525
Fragments arranged by size:
2907 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5963 5963
Size: 5968
BlpI GC'TnA_GC
Cuts at: 3385 3385
Size: 5968
Bme1580I G_kGCm'C
Cuts at: 1790 4029 5185 1790
Size: 2239 1156 2573
Fragments arranged by size:
2573 2239 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5968
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2183 3699 4657
Size: 416 53 83 399 24 766 1516 958
Cuts at: 4657 442
Size: 1753
Fragments arranged by size:
1753 1516 958 766 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2514 3108 4628 1239
Size: 1275 594 1520 2579
Fragments arranged by size:
2579 1520 1275 594
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4093 4958 5256 4093
Size: 865 298 4805
Fragments arranged by size:
4805 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4631 882
Size: 617 3132 2219
Fragments arranged by size:
3132 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3759 493
Size: 711 2555 2702
Fragments arranged by size:
2702 2555 711
BsaBI GATnn'nnATC
Cuts at: 2631 5962 2631
Size: 3331 2637
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3775 4157 4792 275
Size: 53 83 186 936 2242 382 635 1451
Fragments arranged by size:
2242 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4401 4799 5073 5220 5631 4401
Size: 398 274 147 411 4738
Fragments arranged by size:
4738 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5743
Fragments arranged by size:
5743 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 1186
Size: 1285 4683
BseYI C'CCAG_C
Cuts at: 5171 5667 5171
Size: 496 5472
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3143 1163
Size: 573 1407 3988
Fragments arranged by size:
3988 1407 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3418 4179 5207 1540
Size: 275 186 1417 761 1028 2301
Fragments arranged by size:
2301 1417 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2952 4029 4114 5185 2952
Size: 1077 85 1071 3735
Fragments arranged by size:
3735 1077 1071 85
BsmI GAATG_Cn'
Cuts at: 3479 3572 3479
Size: 93 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2952 3287 4029 4114 5185 1391
Size: 399 1162 335 742 85 1071 2174
Fragments arranged by size:
2174 1162 1071 742 399 335 85
BspEI T'CCGG_A
Cuts at: 5631 5631
Size: 5968
BspHI T'CATG_A
Cuts at: 3857 5587 3857
Size: 1730 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3323 844
Size: 2479 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3855 1394
Size: 429 2032 3507
Fragments arranged by size:
3507 2032 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2829 2991 3145 4463 4637 65
Size: 1183 1581 162 154 1318 174 1396
Fragments arranged by size:
1581 1396 1318 1183 174 162 154
BsrFI r'CCGG_y
Cuts at: 1700 1810 4612 4799 1700
Size: 110 2802 187 2869
Fragments arranged by size:
2869 2802 187 110
BsrGI T'GTAC_A
Cuts at: 96 2351 96
Size: 2255 3713
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5968
BssSI C'ACGA_G
Cuts at: 4028 5040 4028
Size: 1012 4956
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5968
BstBI TT'CG_AA
Cuts at: 2760 4772 2760
Size: 2012 3956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5968
BstYI r'GATC_y
Cuts at: 1972 2632 3298 3665 4050 4067 5519 5605 5617
Size: 660 666 367 385 17 1452 86 12
Cuts at: 5617 5963 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 666 660 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4477
Fragments arranged by size:
4477 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2038 2746 487
Size: 683 56 812 708 3709
Fragments arranged by size:
3709 812 708 683 56
BtsI GCAGTG_nn'
Cuts at: 3484 4278 4298 3484
Size: 794 20 5154
Fragments arranged by size:
5154 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5933
DraI TTT'AAA
Cuts at: 3272 3272
Size: 5968
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 809
Size: 507 3659 1802
Fragments arranged by size:
3659 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3415 4304 5933
Size: 54 1425 50 275 18 1585 889 1629
Cuts at: 5933 8
Size: 43
Fragments arranged by size:
1629 1585 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3415 1537
Size: 275 1603 4090
Fragments arranged by size:
4090 1603 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2236 3898 1353
Size: 883 1662 3423
Fragments arranged by size:
3423 1662 883
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4555 4929 5075 387
Size: 1064 138 2966 374 146 1280
Fragments arranged by size:
2966 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2514 3108 4031 4628 5415 5683 1239
Size: 393 882 594 923 597 787 268 1524
Fragments arranged by size:
1524 923 882 787 597 594 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2496 2496
Size: 5968
EcoO109I rG'GnC_Cy
Cuts at: 2035 2035
Size: 5968
EcoRI G'AATT_C
Cuts at: 2694 3277 3655 2694
Size: 583 378 5007
Fragments arranged by size:
5007 583 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2943 2943
Size: 5968
FspI TGC'GCA
Cuts at: 4474 4474
Size: 5968
HaeII r_GCGC'y
Cuts at: 1678 5115 1678
Size: 3437 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2166 2198 2739 2771 4609
Size: 32 135 32 594 32 541 32 1838
Cuts at: 4609 4641 4683 4715 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1838 594 541 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3312 3558 669
Size: 988 281 1374 246 3079
Fragments arranged by size:
3079 1374 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5968
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KpnI G_GTAC'C
Cuts at: 3293 3293
Size: 5968
MfeI C'AATT_G
Cuts at: 3567 3567
Size: 5968
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2851 5082 5266 1305
Size: 1546 2231 184 2007
Fragments arranged by size:
2231 2007 1546 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4146
Fragments arranged by size:
4146 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2467 3926 4285 4444 518
Size: 648 1023 278 1459 359 159 2042
Fragments arranged by size:
2042 1459 1023 648 359 278 159
MspA1I CmG'CkG
Cuts at: 1606 2012 3384 4061 5209 5454 1606
Size: 406 1372 677 1148 245 2120
Fragments arranged by size:
2120 1372 1148 677 406 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4477
Fragments arranged by size:
4477 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5968
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5968
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 3326 4871 1132
Size: 97 2097 1545 2229
Fragments arranged by size:
2229 2097 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5968
PciI A'CATG_T
Cuts at: 4867 4867
Size: 5968
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5968
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 5550 5582 4037
Size: 32 1481 32 4423
Fragments arranged by size:
4423 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2035 2035
Size: 5968
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5968
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PstI C_TGCA'G
Cuts at: 830 2033 2200 3320 830
Size: 1203 167 1120 3478
Fragments arranged by size:
3478 1203 1120 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5968
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SapI GCTCTTCn'nnn_
Cuts at: 2236 2236
Size: 5968
SbfI CC_TGCA'GG
Cuts at: 3320 3320
Size: 5968
ScaI AGT'ACT
Cuts at: 4216 5928 4216
Size: 1712 4256
SfcI C'TryA_G
Cuts at: 826 1045 2029 2196 2850 3162 3316 4451 5132
Size: 219 984 167 654 312 154 1135 681
Cuts at: 5132 5323 826
Size: 191 1471
Fragments arranged by size:
1471 1135 984 681 654 312 219 191
167 154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 3295 1872
Size: 1423 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4072 4973 5235 820
Size: 197 926 2129 901 262 1553
Fragments arranged by size:
2129 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5968
SphI G_CATG'C
Cuts at: 1132 3326 1132
Size: 2194 3774
SspI AAT'ATT
Cuts at: 5 52 3892 5
Size: 47 3840 2081
Fragments arranged by size:
3840 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5968
StyI C'CwwG_G
Cuts at: 513 1065 2004 3407 513
Size: 552 939 1403 3074
Fragments arranged by size:
3074 1403 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3995 4012 4165 4350 4795 325
Size: 1255 2415 17 153 185 445 1498
Fragments arranged by size:
2415 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2351 3258 4214
Size: 274 80 33 51 1443 374 907 956
Cuts at: 4214 5926 96
Size: 1712 138
Fragments arranged by size:
1712 1443 956 907 374 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2260 2292 2658 2690 5928 5960 2260
Size: 32 366 32 3238 32 2268
Fragments arranged by size:
3238 2268 366 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5968
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2911 2911
Size: 5968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5968
XmaI C'CCGG_G
Cuts at: 1870 3293 1870
Size: 1423 4545
XmnI GAAnn'nnTTC
Cuts at: 2047 2927 4097 2047
Size: 880 1170 3918
Fragments arranged by size:
3918 1170 880
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3776 276
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI