(Circular) (Six-base) MAPSORT of: pFN21AE4405.seq Check: 3430 from: 1 to: 5962 pFN21AE4405 5962 bp With 182 enzymes: SgfI * March 28, 2012 13:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2143 2143 Size: 5962 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2680 2886 4025 5409 5677 1632 Size: 1048 206 1139 1384 268 1917 Fragments arranged by size: 1917 1384 1139 1048 268 206 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 2134 3705 5277 2134 Size: 1571 1572 2819 Fragments arranged by size: 2819 1572 1571 ApaI G_GGCC'C Cuts at: 2164 2164 Size: 5962 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 2524 2535 2617 3112 3176 3271 3497 3649 Size: 1031 11 82 495 64 95 226 152 Cuts at: 3649 5758 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1031 495 226 152 95 82 64 11 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2138 2165 3287 1870 Size: 73 89 106 27 1122 4545 Fragments arranged by size: 4545 1122 106 89 73 27 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3283 4738 618 Size: 325 217 627 1496 1455 1842 Fragments arranged by size: 1842 1496 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2039 2164 3281 1391 Size: 648 125 1117 4072 Fragments arranged by size: 4072 1117 648 125 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3111 3264 928 Size: 971 1212 153 3626 Fragments arranged by size: 3626 1212 971 153 BbvCI CC'TCA_GC Cuts at: 2023 2023 Size: 5962 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2872 3854 5064 2872 Size: 982 1210 3770 Fragments arranged by size: 3770 1210 982 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2043 4573 136 Size: 107 122 71 631 605 371 2530 1525 Fragments arranged by size: 2530 1525 631 605 371 122 107 71 BglII A'GATC_T Cuts at: 3119 5957 3119 Size: 2838 3124 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 2164 4023 5179 1790 Size: 374 1859 1156 2573 Fragments arranged by size: 2573 1859 1156 374 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4622 1239 Size: 3383 2579 Bpu10I CC'TnA_GC Cuts at: 2023 2023 Size: 5962 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2811 4087 4952 5250 2811 Size: 1276 865 298 3523 Fragments arranged by size: 3523 1276 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2898 4625 882 Size: 617 1399 1727 2219 Fragments arranged by size: 2219 1727 1399 617 BsaAI yAC'GTr Cuts at: 493 1204 3753 493 Size: 711 2549 2702 Fragments arranged by size: 2702 2549 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3186 4395 4793 5067 5214 5625 3186 Size: 1209 398 274 147 411 3523 Fragments arranged by size: 3523 1209 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 1178 Size: 30 165 30 867 30 4840 Fragments arranged by size: 4840 867 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2126 2183 2186 2189 2192 2195 2198 Size: 907 33 57 3 3 3 3 3 Cuts at: 2198 2201 2967 1186 Size: 3 766 4181 Fragments arranged by size: 4181 907 766 57 33 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2934 5165 5661 2934 Size: 2231 496 3235 Fragments arranged by size: 3235 2231 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2171 1163 Size: 573 435 4954 Fragments arranged by size: 4954 573 435 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2284 3412 4173 5201 1540 Size: 275 186 283 1128 761 1028 2301 Fragments arranged by size: 2301 1128 1028 761 283 275 186 BsiHKAI G_wGCw'C Cuts at: 4023 4108 5179 4023 Size: 85 1071 4806 Fragments arranged by size: 4806 1071 85 BsmI GAATG_Cn' Cuts at: 2129 3473 3566 2129 Size: 1344 93 4525 Fragments arranged by size: 4525 1344 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2164 3281 4023 4108 5179 1391 Size: 399 249 125 1117 742 85 1071 2174 Fragments arranged by size: 2174 1117 1071 742 399 249 125 85 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 2509 3851 5581 2509 Size: 1342 1730 2890 Fragments arranged by size: 2890 1730 1342 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2143 3317 844 Size: 1299 1174 3489 Fragments arranged by size: 3489 1299 1174 BsrBI CCG'CTC Cuts at: 1394 1823 2286 3849 1394 Size: 429 463 1563 3507 Fragments arranged by size: 3507 1563 463 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2228 3049 4606 4793 1700 Size: 110 418 821 1557 187 2869 Fragments arranged by size: 2869 1557 821 418 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 2121 4766 2121 Size: 2645 3317 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstYI r'GATC_y Cuts at: 1972 3119 3292 3659 4044 4061 5513 5599 5611 Size: 1147 173 367 385 17 1452 86 12 Cuts at: 5611 5957 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1147 385 367 346 173 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2039 2220 513 Size: 552 939 35 181 4255 Fragments arranged by size: 4255 939 552 181 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 3478 4272 4292 3478 Size: 794 20 5148 Fragments arranged by size: 5148 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 2575 3266 2575 Size: 691 5271 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2343 4969 809 Size: 507 1027 2626 1802 Fragments arranged by size: 2626 1802 1027 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2205 2281 3409 Size: 54 1425 50 275 18 375 76 1128 Cuts at: 3409 4298 5927 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1128 889 375 275 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2281 3409 1537 Size: 275 469 1128 4090 Fragments arranged by size: 4090 1128 469 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2959 3004 3892 1353 Size: 1606 45 888 3423 Fragments arranged by size: 3423 1606 888 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4549 4923 5069 387 Size: 1064 138 435 2525 374 146 1280 Fragments arranged by size: 2525 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2680 2886 4025 4622 5409 5677 1239 Size: 393 1048 206 1139 597 787 268 1524 Fragments arranged by size: 1524 1139 1048 787 597 393 268 206 EcoO109I rG'GnC_Cy Cuts at: 2160 2161 2304 2160 Size: 1 143 5818 Fragments arranged by size: 5818 143 1 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 5109 1678 Size: 3431 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 2270 2302 2644 Size: 32 135 32 434 32 232 32 342 Cuts at: 2644 2676 4603 4635 4677 4709 1373 Size: 32 1927 32 42 32 2626 Fragments arranged by size: 2626 1927 434 342 232 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 2484 3561 2484 Size: 1077 4885 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5076 5260 1305 Size: 3771 184 2007 Fragments arranged by size: 3771 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3920 4279 4438 518 Size: 648 2754 359 159 2042 Fragments arranged by size: 2754 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 2041 2783 3378 4055 5203 5448 1606 Size: 421 14 742 595 677 1148 245 2120 Fragments arranged by size: 2120 1148 742 677 595 421 245 14 NaeI GCC'GGC Cuts at: 2230 2230 Size: 5962 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4471 Fragments arranged by size: 4471 939 552 NdeI CA'TA_TG Cuts at: 387 2378 2625 387 Size: 1991 247 3724 Fragments arranged by size: 3724 1991 247 NgoMIV G'CCGG_C Cuts at: 2228 2228 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 3320 4865 1132 Size: 97 2091 1545 2229 Fragments arranged by size: 2229 2091 1545 97 PacI TTA_AT'TAA Cuts at: 2532 2532 Size: 5962 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 2713 1108 Size: 1605 4357 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2102 2134 4031 4063 5544 5576 2102 Size: 32 1897 32 1481 32 2488 Fragments arranged by size: 2488 1897 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2304 2304 Size: 5962 PshAI GACnn'nnGTC Cuts at: 1613 3194 1613 Size: 1581 4381 PsiI TTA'TAA Cuts at: 3174 3532 3174 Size: 358 5604 PspOMI G'GGCC_C Cuts at: 2160 2160 Size: 5962 PspXI vC'TCGA_Gb Cuts at: 2138 2138 Size: 5962 PstI C_TGCA'G Cuts at: 830 3314 830 Size: 2484 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 SacII CC_GC'GG Cuts at: 2042 2042 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SapI GCTCTTCn'nnn_ Cuts at: 3004 3004 Size: 5962 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2544 3310 4445 5126 5317 826 Size: 219 1499 766 1135 681 191 1471 Fragments arranged by size: 1499 1471 1135 766 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2138 2826 4066 4967 5229 820 Size: 197 926 195 688 1240 901 262 1553 Fragments arranged by size: 1553 1240 926 901 688 262 197 195 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 2604 3886 5 Size: 47 2552 1282 2081 Fragments arranged by size: 2552 2081 1282 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 2004 2454 3401 513 Size: 552 939 450 947 3074 Fragments arranged by size: 3074 947 939 552 450 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3989 4006 4159 4344 4789 325 Size: 1255 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2984 4208 5920 Size: 274 80 33 51 1443 1007 1224 1712 Cuts at: 5920 96 Size: 138 Fragments arranged by size: 1712 1443 1224 1007 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 2138 1943 Size: 195 5767 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 2906 4091 2906 Size: 1185 4777 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PfoI PmlI PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI