Restriction map for
pFN21AE4405
(Circular) (Six-base) MAPSORT of: pFN21AE4405.seq Check: 3430 from: 1 to: 5962
pFN21AE4405 5962 bp
With 182 enzymes: SgfI *
March 28, 2012 13:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2143 2143
Size: 5962
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2680 2886 4025 5409 5677 1632
Size: 1048 206 1139 1384 268 1917
Fragments arranged by size:
1917 1384 1139 1048 268 206
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 2134 3705 5277 2134
Size: 1571 1572 2819
Fragments arranged by size:
2819 1572 1571
ApaI G_GGCC'C
Cuts at: 2164 2164
Size: 5962
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 2524 2535 2617 3112 3176 3271 3497 3649
Size: 1031 11 82 495 64 95 226 152
Cuts at: 3649 5758 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1031 495 226 152 95 82
64 11
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2138 2165 3287 1870
Size: 73 89 106 27 1122 4545
Fragments arranged by size:
4545 1122 106 89 73 27
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3283 4738 618
Size: 325 217 627 1496 1455 1842
Fragments arranged by size:
1842 1496 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2039 2164 3281 1391
Size: 648 125 1117 4072
Fragments arranged by size:
4072 1117 648 125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3111 3264 928
Size: 971 1212 153 3626
Fragments arranged by size:
3626 1212 971 153
BbvCI CC'TCA_GC
Cuts at: 2023 2023
Size: 5962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2872 3854 5064 2872
Size: 982 1210 3770
Fragments arranged by size:
3770 1210 982
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2043 4573 136
Size: 107 122 71 631 605 371 2530 1525
Fragments arranged by size:
2530 1525 631 605 371 122 107 71
BglII A'GATC_T
Cuts at: 3119 5957 3119
Size: 2838 3124
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 2164 4023 5179 1790
Size: 374 1859 1156 2573
Fragments arranged by size:
2573 1859 1156 374
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4622 1239
Size: 3383 2579
Bpu10I CC'TnA_GC
Cuts at: 2023 2023
Size: 5962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2811 4087 4952 5250 2811
Size: 1276 865 298 3523
Fragments arranged by size:
3523 1276 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2898 4625 882
Size: 617 1399 1727 2219
Fragments arranged by size:
2219 1727 1399 617
BsaAI yAC'GTr
Cuts at: 493 1204 3753 493
Size: 711 2549 2702
Fragments arranged by size:
2702 2549 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3186 4395 4793 5067 5214 5625 3186
Size: 1209 398 274 147 411 3523
Fragments arranged by size:
3523 1209 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 1178
Size: 30 165 30 867 30 4840
Fragments arranged by size:
4840 867 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2126 2183 2186 2189 2192 2195 2198
Size: 907 33 57 3 3 3 3 3
Cuts at: 2198 2201 2967 1186
Size: 3 766 4181
Fragments arranged by size:
4181 907 766 57 33 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2934 5165 5661 2934
Size: 2231 496 3235
Fragments arranged by size:
3235 2231 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2171 1163
Size: 573 435 4954
Fragments arranged by size:
4954 573 435
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2284 3412 4173 5201 1540
Size: 275 186 283 1128 761 1028 2301
Fragments arranged by size:
2301 1128 1028 761 283 275 186
BsiHKAI G_wGCw'C
Cuts at: 4023 4108 5179 4023
Size: 85 1071 4806
Fragments arranged by size:
4806 1071 85
BsmI GAATG_Cn'
Cuts at: 2129 3473 3566 2129
Size: 1344 93 4525
Fragments arranged by size:
4525 1344 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2164 3281 4023 4108 5179 1391
Size: 399 249 125 1117 742 85 1071 2174
Fragments arranged by size:
2174 1117 1071 742 399 249 125 85
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 2509 3851 5581 2509
Size: 1342 1730 2890
Fragments arranged by size:
2890 1730 1342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2143 3317 844
Size: 1299 1174 3489
Fragments arranged by size:
3489 1299 1174
BsrBI CCG'CTC
Cuts at: 1394 1823 2286 3849 1394
Size: 429 463 1563 3507
Fragments arranged by size:
3507 1563 463 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2228 3049 4606 4793 1700
Size: 110 418 821 1557 187 2869
Fragments arranged by size:
2869 1557 821 418 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 2121 4766 2121
Size: 2645 3317
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 3119 3292 3659 4044 4061 5513 5599 5611
Size: 1147 173 367 385 17 1452 86 12
Cuts at: 5611 5957 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1147 385 367 346 173 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2039 2220 513
Size: 552 939 35 181 4255
Fragments arranged by size:
4255 939 552 181 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 3478 4272 4292 3478
Size: 794 20 5148
Fragments arranged by size:
5148 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 2575 3266 2575
Size: 691 5271
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2343 4969 809
Size: 507 1027 2626 1802
Fragments arranged by size:
2626 1802 1027 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2281 3409
Size: 54 1425 50 275 18 375 76 1128
Cuts at: 3409 4298 5927 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1128 889 375 275 76 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2281 3409 1537
Size: 275 469 1128 4090
Fragments arranged by size:
4090 1128 469 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2959 3004 3892 1353
Size: 1606 45 888 3423
Fragments arranged by size:
3423 1606 888 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4549 4923 5069 387
Size: 1064 138 435 2525 374 146 1280
Fragments arranged by size:
2525 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2680 2886 4025 4622 5409 5677 1239
Size: 393 1048 206 1139 597 787 268 1524
Fragments arranged by size:
1524 1139 1048 787 597 393 268 206
EcoO109I rG'GnC_Cy
Cuts at: 2160 2161 2304 2160
Size: 1 143 5818
Fragments arranged by size:
5818 143 1
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 5109 1678
Size: 3431 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2270 2302 2644
Size: 32 135 32 434 32 232 32 342
Cuts at: 2644 2676 4603 4635 4677 4709 1373
Size: 32 1927 32 42 32 2626
Fragments arranged by size:
2626 1927 434 342 232 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 2484 3561 2484
Size: 1077 4885
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5076 5260 1305
Size: 3771 184 2007
Fragments arranged by size:
3771 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3920 4279 4438 518
Size: 648 2754 359 159 2042
Fragments arranged by size:
2754 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 2041 2783 3378 4055 5203 5448 1606
Size: 421 14 742 595 677 1148 245 2120
Fragments arranged by size:
2120 1148 742 677 595 421 245 14
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 5962
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4471
Fragments arranged by size:
4471 939 552
NdeI CA'TA_TG
Cuts at: 387 2378 2625 387
Size: 1991 247 3724
Fragments arranged by size:
3724 1991 247
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 3320 4865 1132
Size: 97 2091 1545 2229
Fragments arranged by size:
2229 2091 1545 97
PacI TTA_AT'TAA
Cuts at: 2532 2532
Size: 5962
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2713 1108
Size: 1605 4357
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2102 2134 4031 4063 5544 5576 2102
Size: 32 1897 32 1481 32 2488
Fragments arranged by size:
2488 1897 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2304 2304
Size: 5962
PshAI GACnn'nnGTC
Cuts at: 1613 3194 1613
Size: 1581 4381
PsiI TTA'TAA
Cuts at: 3174 3532 3174
Size: 358 5604
PspOMI G'GGCC_C
Cuts at: 2160 2160
Size: 5962
PspXI vC'TCGA_Gb
Cuts at: 2138 2138
Size: 5962
PstI C_TGCA'G
Cuts at: 830 3314 830
Size: 2484 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
SacII CC_GC'GG
Cuts at: 2042 2042
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SapI GCTCTTCn'nnn_
Cuts at: 3004 3004
Size: 5962
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2544 3310 4445 5126 5317 826
Size: 219 1499 766 1135 681 191 1471
Fragments arranged by size:
1499 1471 1135 766 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2138 2826 4066 4967 5229 820
Size: 197 926 195 688 1240 901 262 1553
Fragments arranged by size:
1553 1240 926 901 688 262 197 195
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 2604 3886 5
Size: 47 2552 1282 2081
Fragments arranged by size:
2552 2081 1282 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 2004 2454 3401 513
Size: 552 939 450 947 3074
Fragments arranged by size:
3074 947 939 552 450
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3989 4006 4159 4344 4789 325
Size: 1255 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2984 4208 5920
Size: 274 80 33 51 1443 1007 1224 1712
Cuts at: 5920 96
Size: 138
Fragments arranged by size:
1712 1443 1224 1007 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 2138 1943
Size: 195 5767
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 2906 4091 2906
Size: 1185 4777
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PacI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PfoI PmlI PsrI PvuII RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI XcmI