Restriction map for
pFN21AE4404
(Circular) (Six-base) MAPSORT of: pFN21AE4404.seq Check: 6897 from: 1 to: 5962
pFN21AE4404 5962 bp
With 182 enzymes: SgfI *
March 28, 2012 13:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2817 2817
Size: 5962
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2619 3772 278
Size: 53 83 186 936 1083 1153 2468
Fragments arranged by size:
2468 1153 1083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2240 2914 3071 4025 5409 5677 1632
Size: 422 186 674 157 954 1384 268 1917
Fragments arranged by size:
1917 1384 954 674 422 268 186 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 3151 4861 5838 1203
Size: 1948 1710 977 1327
Fragments arranged by size:
1948 1710 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 2235 2516 1166
Size: 1069 281 4612
Fragments arranged by size:
4612 1069 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2777 1184
Size: 1593 4369
AlwNI CAG_nnn'CTG
Cuts at: 2593 2757 2836 3705 5277 2593
Size: 164 79 869 1572 3278
Fragments arranged by size:
3278 1572 869 164 79
ApaI G_GGCC'C
Cuts at: 2697 2697
Size: 5962
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 2291 2569 2624 3271 3497 3649 5758 1493
Size: 798 278 55 647 226 152 2109 1697
Fragments arranged by size:
2109 1697 798 647 278 226 152 55
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 2466 3034 3287 1870
Size: 73 226 297 568 253 4545
Fragments arranged by size:
4545 568 297 253 226 73
AvrII C'CTAG_G
Cuts at: 2914 2914
Size: 5962
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2136 3121 3283 4738 618
Size: 325 217 627 349 985 162 1455 1842
Fragments arranged by size:
1842 1455 985 627 349 325 217 162
BanII G_rGCy'C
Cuts at: 1391 2525 2697 3281 1391
Size: 1134 172 584 4072
Fragments arranged by size:
4072 1134 584 172
BbeI G_GCGC'C
Cuts at: 2140 2140
Size: 5962
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2504 928
Size: 971 605 4386
Fragments arranged by size:
4386 971 605
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4153 4187 1903
Size: 34 50 34 2132 34 3678
Fragments arranged by size:
3678 2132 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 2148 1548
Size: 600 5362
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 2195 2765 2813 3379 2195
Size: 570 48 566 4778
Fragments arranged by size:
4778 570 566 48
Bme1580I G_kGCm'C
Cuts at: 1790 2084 2429 2697 4023 5179 1790
Size: 294 345 268 1326 1156 2573
Fragments arranged by size:
2573 1326 1156 345 294 268
BmgBI CAC'GTC
Cuts at: 1318 2675 1318
Size: 1357 4605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2126 2891 3693
Size: 416 53 83 399 24 709 765 802
Cuts at: 3693 4651 442
Size: 958 1753
Fragments arranged by size:
1753 958 802 765 709 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2477 1056
Size: 1421 4541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 2764 2765 2867 4622 1239
Size: 1238 287 1 102 1755 2579
Fragments arranged by size:
2579 1755 1238 287 102 1
Bpu10I CC'TnA_GC
Cuts at: 3130 3130
Size: 5962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4087 4952 5250 4087
Size: 865 298 4799
Fragments arranged by size:
4799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4625 882
Size: 617 3126 2219
Fragments arranged by size:
3126 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3061 3753 493
Size: 711 1857 692 2702
Fragments arranged by size:
2702 1857 711 692
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2137 2533 2616 3769
Size: 53 83 186 936 604 396 83 1153
Cuts at: 3769 4151 4786 275
Size: 382 635 1451
Fragments arranged by size:
1451 1153 936 635 604 396 382 186
83 83 53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2377 2407 1178
Size: 30 165 30 974 30 4733
Fragments arranged by size:
4733 974 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3271 1186
Size: 2085 3877
BseYI C'CCAG_C
Cuts at: 2300 2735 5165 5661 2300
Size: 435 2430 496 2601
Fragments arranged by size:
2601 2430 496 435
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3212 1163
Size: 573 1476 3913
Fragments arranged by size:
3913 1476 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4173 5201 1540
Size: 275 186 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2751 4023 4108 5179 2751
Size: 1272 85 1071 3534
Fragments arranged by size:
3534 1272 1071 85
BsmI GAATG_Cn'
Cuts at: 2129 2661 3473 3566 2129
Size: 532 812 93 4525
Fragments arranged by size:
4525 812 532 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2607 2607
Size: 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2429 2525 2697 2751 3281 4023
Size: 399 294 345 96 172 54 530 742
Cuts at: 4023 4108 5179 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 530 399 345 294 172
96 85 54
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 2988 3851 5581 2988
Size: 863 1730 3369
Fragments arranged by size:
3369 1730 863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2691 2817 3317 844
Size: 1847 126 500 3489
Fragments arranged by size:
3489 1847 500 126
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2556 4606 4793 1700
Size: 110 746 2050 187 2869
Fragments arranged by size:
2869 2050 746 187 110
BsrGI T'GTAC_A
Cuts at: 96 2508 96
Size: 2412 3550
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 3292 3659 4044 4061 5513 5599 5611 5957
Size: 1320 367 385 17 1452 86 12 346
Cuts at: 5957 1972
Size: 1977
Fragments arranged by size:
1977 1452 1320 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5410
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 3478 4272 4292 3478
Size: 794 20 5148
Fragments arranged by size:
5148 794 20
ClaI AT'CG_AT
Cuts at: 1554 2631 1554
Size: 1077 4885
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2269 2304 529
Size: 35 1705 35 4187
Fragments arranged by size:
4187 1705 35 35
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2606 4969 809
Size: 507 1290 2363 1802
Fragments arranged by size:
2363 1802 1290 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2269 3409 4298
Size: 54 1425 50 275 18 439 1140 889
Cuts at: 4298 5927 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1140 889 439 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3892 1353
Size: 2539 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2240 2477 2764 2765 2867 2914
Size: 393 422 186 237 287 1 102 47
Cuts at: 2914 3071 4025 4622 5409 5677 1239
Size: 157 954 597 787 268 1524
Fragments arranged by size:
1524 954 787 597 422 393 287 268
237 186 157 102 47 1
EcoO109I rG'GnC_Cy
Cuts at: 2694 2694
Size: 5962
EcoRI G'AATT_C
Cuts at: 2569 2624 3271 3649 2569
Size: 55 647 378 4882
Fragments arranged by size:
4882 647 378 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 5962
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 2140 5109 1678
Size: 462 2969 2531
Fragments arranged by size:
2969 2531 462
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2377 2409 3094 3126 4603
Size: 32 135 32 805 32 685 32 1477
Cuts at: 4603 4635 4677 4709 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1477 805 685 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3007 3306 3552 669
Size: 988 281 1069 299 246 3079
Fragments arranged by size:
3079 1069 988 299 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KasI G'GCGC_C
Cuts at: 2136 2136
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2137 5076 5260 1305
Size: 832 2939 184 2007
Fragments arranged by size:
2939 2007 832 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2235 2265 2516 3920 4279 4438 518
Size: 648 1069 30 251 1404 359 159 2042
Fragments arranged by size:
2042 1404 1069 648 359 251 159 30
MspA1I CmG'CkG
Cuts at: 1606 2516 3085 3378 4055 5203 5448 1606
Size: 910 569 293 677 1148 245 2120
Fragments arranged by size:
2120 1148 910 677 569 293 245
NaeI GCC'GGC
Cuts at: 2558 2558
Size: 5962
NarI GG'CG_CC
Cuts at: 2137 2137
Size: 5962
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5410
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NgoMIV G'CCGG_C
Cuts at: 2556 2556
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 2473 1052
Size: 1421 4541
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 3155 3320 4865 1132
Size: 97 1926 165 1545 2229
Fragments arranged by size:
2229 1926 1545 165 97
PasI CC'CwG_GG
Cuts at: 1348 2422 1348
Size: 1074 4888
PciI A'CATG_T
Cuts at: 3151 4861 3151
Size: 1710 4252
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PfoI T'CCnGG_A
Cuts at: 2454 2619 2844 2454
Size: 165 225 5572
Fragments arranged by size:
5572 225 165
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PspOMI G'GGCC_C
Cuts at: 2693 2693
Size: 5962
PstI C_TGCA'G
Cuts at: 830 3314 830
Size: 2484 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2516 3085 2516
Size: 569 5393
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 3225 3310 4445 5126 5317 826
Size: 219 2180 85 1135 681 191 1471
Fragments arranged by size:
2180 1471 1135 681 219 191 85
SfoI GGC'GCC
Cuts at: 2138 2138
Size: 5962
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 2556 1700
Size: 856 5106
SmaI CCC'GGG
Cuts at: 1872 3036 3289 1872
Size: 1164 253 4545
Fragments arranged by size:
4545 1164 253
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2169 2466 4066 4967 5229 820
Size: 197 926 226 297 1600 901 262 1553
Fragments arranged by size:
1600 1553 926 901 297 262 226 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 2913 1517
Size: 1396 4566
StyI C'CwwG_G
Cuts at: 513 1065 2396 2908 2914 2949 3401 513
Size: 552 1331 512 6 35 452 3074
Fragments arranged by size:
3074 1331 552 512 452 35 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2063 2448 2616 2811 3989 4006 4159
Size: 1255 483 385 168 195 1178 17 153
Cuts at: 4159 4344 4789 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1178 483 445 385 195 185
168 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2508 4208 5920
Size: 274 80 33 51 1443 531 1700 1712
Cuts at: 5920 96
Size: 138
Fragments arranged by size:
1712 1700 1443 531 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2145 2177 5922 5954 2145
Size: 32 3745 32 2153
Fragments arranged by size:
3745 2153 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 2169 2466 1943
Size: 226 297 5439
Fragments arranged by size:
5439 297 226
XmaI C'CCGG_G
Cuts at: 1870 3034 3287 1870
Size: 1164 253 4545
Fragments arranged by size:
4545 1164 253
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2617 3770 276
Size: 53 83 186 936 1083 1153 2468
Fragments arranged by size:
2468 1153 1083 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI