Restriction map for
pFN21AE4403
(Circular) (Six-base) MAPSORT of: pFN21AE4403.seq Check: 9810 from: 1 to: 5962
pFN21AE4403 5962 bp
With 182 enzymes: SgfI *
March 28, 2012 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 2091 3283 2091
Size: 1192 4770
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4025 5409 5677 1632
Size: 2393 1384 268 1917
Fragments arranged by size:
2393 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 2714 4861 5838 1203
Size: 1511 2147 977 1327
Fragments arranged by size:
2147 1511 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 2344 4691 2344
Size: 2347 3615
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2880 2912 2880
Size: 32 5930
AlwNI CAG_nnn'CTG
Cuts at: 3705 5277 3705
Size: 1572 4390
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2493 2547 3287 1870
Size: 73 550 54 740 4545
Fragments arranged by size:
4545 740 550 73 54
AvrII C'CTAG_G
Cuts at: 2138 2935 2138
Size: 797 5165
BamHI G'GATC_C
Cuts at: 3000 3292 3659 3000
Size: 292 367 5303
Fragments arranged by size:
5303 367 292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2091 3229 3283 4738
Size: 325 217 627 282 22 1138 54 1455
Cuts at: 4738 618
Size: 1842
Fragments arranged by size:
1842 1455 1138 627 325 282 217 54
22
BanII G_rGCy'C
Cuts at: 1391 3281 1391
Size: 1890 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2481 928
Size: 971 582 4409
Fragments arranged by size:
4409 971 582
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2933 3854 5064 2933
Size: 921 1210 3831
Fragments arranged by size:
3831 1210 921
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BfrBI ATG'CAT
Cuts at: 2444 2444
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 2525 3011 3379 2525
Size: 486 368 5108
Fragments arranged by size:
5108 486 368
Bme1580I G_kGCm'C
Cuts at: 1790 2072 4023 5179 1790
Size: 282 1951 1156 2573
Fragments arranged by size:
2573 1951 1156 282
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2550 2550
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2304 2520 2612 3041 4622 1239
Size: 1065 216 92 429 1581 2579
Fragments arranged by size:
2579 1581 1065 429 216 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2585 4087 4952 5250 2585
Size: 1502 865 298 3297
Fragments arranged by size:
3297 1502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4625 882
Size: 617 3126 2219
Fragments arranged by size:
3126 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2482 3753 493
Size: 711 1278 1271 2702
Fragments arranged by size:
2702 1278 1271 711
BsaBI GATnn'nnATC
Cuts at: 3164 5956 3164
Size: 2792 3170
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2906 2936 1178
Size: 30 165 30 1503 30 4204
Fragments arranged by size:
4204 1503 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 2813 1186
Size: 1171 456 4335
Fragments arranged by size:
4335 1171 456
BseYI C'CCAG_C
Cuts at: 2720 2910 5165 5661 2720
Size: 190 2255 496 3021
Fragments arranged by size:
3021 2255 496 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2527 1163
Size: 573 791 4598
Fragments arranged by size:
4598 791 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3096 3412 4173 5201 1540
Size: 275 186 1095 316 761 1028 2301
Fragments arranged by size:
2301 1095 1028 761 316 275 186
BsiHKAI G_wGCw'C
Cuts at: 4023 4108 5179 4023
Size: 85 1071 4806
Fragments arranged by size:
4806 1071 85
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 3281 4023 4108 5179 1391
Size: 399 282 1209 742 85 1071 2174
Fragments arranged by size:
2174 1209 1071 742 399 282 85
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 3851 5581 3851
Size: 1730 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 2181 3317 844
Size: 1259 78 1136 3489
Fragments arranged by size:
3489 1259 1136 78
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2047 4457 4631 65
Size: 1183 799 2410 174 1396
Fragments arranged by size:
2410 1396 1183 799 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 4606 4793 1700
Size: 110 293 2503 187 2869
Fragments arranged by size:
2869 2503 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2482 794
Size: 1688 4274
BstBI TT'CG_AA
Cuts at: 2960 4766 2960
Size: 1806 4156
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstXI CCAn_nnnn'nTGG
Cuts at: 3065 3065
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 2434 3000 3036 3292 3659 4044 4061 5513
Size: 462 566 36 256 367 385 17 1452
Cuts at: 5513 5599 5611 5957 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 566 462 385 367 346 256
86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 2518 3090 513
Size: 552 939 60 454 572 3385
Fragments arranged by size:
3385 939 572 552 454 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 2583 3084 3478 4272 4292 2583
Size: 501 394 794 20 4253
Fragments arranged by size:
4253 794 501 394 20
ClaI AT'CG_AT
Cuts at: 1554 2670 3168 1554
Size: 1116 498 4348
Fragments arranged by size:
4348 1116 498
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2035 2196
Size: 54 1425 50 275 18 177 28 161
Cuts at: 2196 2299 2506 2577 2617 2738 3409 4298 5927
Size: 103 207 71 40 121 671 889 1629
Cuts at: 5927 8
Size: 43
Fragments arranged by size:
1629 1425 889 671 275 207 177 161
121 103 71 54 50 43 40 28
18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3892 1353
Size: 2539 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2304 2520 2612 3041 4025 4622 5409
Size: 393 672 216 92 429 984 597 787
Cuts at: 5409 5677 1239
Size: 268 1524
Fragments arranged by size:
1524 984 787 672 597 429 393 268
216 92
EcoO109I rG'GnC_Cy
Cuts at: 2596 2608 3019 2596
Size: 12 411 5539
Fragments arranged by size:
5539 411 12
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FspI TGC'GCA
Cuts at: 2177 2501 4468 2177
Size: 324 1967 3671
Fragments arranged by size:
3671 1967 324
FspAI rTGC'GCAy
Cuts at: 2501 2501
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 2935 5109 1678
Size: 1257 2174 2531
Fragments arranged by size:
2531 2174 1257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2330 2362 2973 3005 4603
Size: 32 135 32 758 32 611 32 1598
Cuts at: 4603 4635 4677 4709 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1598 758 611 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2228 748
Size: 1480 4482
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 2095 3287 2095
Size: 1192 4770
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3141 3186 5076 5260 1305
Size: 1836 45 1890 184 2007
Fragments arranged by size:
2007 1890 1836 184 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2301 2579 2740 10
Size: 54 1425 343 177 292 278 161 3232
Fragments arranged by size:
3232 1425 343 292 278 177 161 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2766 3920 4279 4438 518
Size: 648 1600 1154 359 159 2042
Fragments arranged by size:
2042 1600 1154 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2662 3092 3378 4055 5203 5448 1606
Size: 1056 430 286 677 1148 245 2120
Fragments arranged by size:
2120 1148 1056 677 430 286 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2064 513
Size: 552 939 60 4411
Fragments arranged by size:
4411 939 552 60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NsiI A_TGCA'T
Cuts at: 2446 2446
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2718 3320 4865 1132
Size: 97 1489 602 1545 2229
Fragments arranged by size:
2229 1545 1489 602 97
PasI CC'CwG_GG
Cuts at: 1348 2412 1348
Size: 1064 4898
PciI A'CATG_T
Cuts at: 2714 4861 2714
Size: 2147 3815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PfoI T'CCnGG_A
Cuts at: 2127 3039 2127
Size: 912 5050
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2608 3019 2608
Size: 411 5551
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 3314 830
Size: 2484 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
SacII CC_GC'GG
Cuts at: 3093 3093
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SexAI A'CCwGG_T
Cuts at: 3183 3183
Size: 5962
SfcI C'TryA_G
Cuts at: 826 1045 3310 4445 5126 5317 826
Size: 219 2265 1135 681 191 1471
Fragments arranged by size:
2265 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 2549 3289 1872
Size: 677 740 4545
Fragments arranged by size:
4545 740 677
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2600 4066 4967 5229 820
Size: 197 926 657 1466 901 262 1553
Fragments arranged by size:
1553 1466 926 901 657 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 2004 2064 2138 2458 2935 2965 3401
Size: 552 939 60 74 320 477 30 436
Cuts at: 3401 513
Size: 3074
Fragments arranged by size:
3074 939 552 477 436 320 74 60
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2263 2471 3989 4006 4159 4344 4789
Size: 1255 683 208 1518 17 153 185 445
Cuts at: 4789 325
Size: 1498
Fragments arranged by size:
1518 1498 1255 683 445 208 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4208 5920 96
Size: 274 80 33 51 1443 2231 1712 138
Fragments arranged by size:
2231 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2716 2748 2906 2938 5922 5954 2716
Size: 32 158 32 2984 32 2724
Fragments arranged by size:
2984 2724 158 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2378 1316
Size: 1062 4900
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2587 2587
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 2547 3287 1870
Size: 677 740 4545
Fragments arranged by size:
4545 740 677
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI
SapI SfiI SfoI SrfI SwaI