Restriction map for
pFN21AE4401
(Circular) (Six-base) MAPSORT of: pFN21AE4401.seq Check: 9550 from: 1 to: 5956
pFN21AE4401 5956 bp
With 182 enzymes: SgfI *
March 28, 2012 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2718 3171 3299 1937
Size: 781 453 128 4594
Fragments arranged by size:
4594 781 453 128
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4019 5403 5671 1632
Size: 2387 1384 268 1917
Fragments arranged by size:
2387 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 2565 1166
Size: 1399 4557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2492 1184
Size: 1308 4648
AlwNI CAG_nnn'CTG
Cuts at: 2395 2857 3699 5271 2395
Size: 462 842 1572 3080
Fragments arranged by size:
3080 1572 842 462
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 3265 3491 3643 5752 1493
Size: 1772 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 2940 3029 3281 1870
Size: 73 997 89 252 4545
Fragments arranged by size:
4545 997 252 89 73
BamHI G'GATC_C
Cuts at: 2573 3286 3653 2573
Size: 713 367 4876
Fragments arranged by size:
4876 713 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2333 2663 3277 4732 618
Size: 325 217 627 546 330 614 1455 1842
Fragments arranged by size:
1842 1455 627 614 546 330 325 217
BanII G_rGCy'C
Cuts at: 1391 3275 1391
Size: 1884 4072
BbeI G_GCGC'C
Cuts at: 2337 2337
Size: 5956
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 2123 2430
Size: 107 122 71 631 605 430 21 307
Cuts at: 2430 2852 3109 4567 136
Size: 422 257 1458 1525
Fragments arranged by size:
1525 1458 631 605 430 422 307 257
122 107 71 21
BglII A'GATC_T
Cuts at: 2871 5951 2871
Size: 3080 2876
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2299 3140 3151 4017 5173 1790
Size: 509 841 11 866 1156 2573
Fragments arranged by size:
2573 1156 866 841 509 11
BmgBI CAC'GTC
Cuts at: 1318 2220 2320 2346 1318
Size: 902 100 26 4928
Fragments arranged by size:
4928 902 100 26
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2156 2602 3687
Size: 416 53 83 399 24 739 446 1085
Cuts at: 3687 4645 442
Size: 958 1753
Fragments arranged by size:
1753 1085 958 739 446 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2579 2425
Size: 154 5802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2029 2420 2779 2915 4616 1239
Size: 790 391 359 136 1701 2579
Fragments arranged by size:
2579 1701 790 391 359 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2334 3101 3168 3763
Size: 53 83 186 936 801 767 67 595
Cuts at: 3763 4145 4780 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 801 767 635 595 382 186
83 67 53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2141 2171 1178
Size: 30 165 30 738 30 4963
Fragments arranged by size:
4963 738 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2227 2291 2774 1186
Size: 937 104 64 483 4368
Fragments arranged by size:
4368 937 483 104 64
BseYI C'CCAG_C
Cuts at: 2698 2839 2889 3249 5159 5655 2698
Size: 141 50 360 1910 496 2999
Fragments arranged by size:
2999 1910 496 360 141 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2726 1163
Size: 573 450 540 4393
Fragments arranged by size:
4393 573 540 450
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2237 2560 4017 4102 5173 2237
Size: 323 1457 85 1071 3020
Fragments arranged by size:
3020 1457 1071 323 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2299 2560 3140 3151 3275 4017
Size: 399 447 62 261 580 11 124 742
Cuts at: 4017 4102 5173 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 580 447 399 261 124
85 62 11
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2158 3311 844
Size: 1314 1153 3489
Fragments arranged by size:
3489 1314 1153
BsrBI CCG'CTC
Cuts at: 1394 1823 2626 3843 1394
Size: 429 803 1217 3507
Fragments arranged by size:
3507 1217 803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2611 2965 2977 4451 4625 65
Size: 1183 1363 354 12 1474 174 1396
Fragments arranged by size:
1474 1396 1363 1183 354 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2520 2660 2846 4600 4787 1700
Size: 110 710 140 186 1754 187 2869
Fragments arranged by size:
2869 1754 710 187 186 140 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 4016 5028 4016
Size: 1012 4944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 3244 1184
Size: 2060 3896
BstXI CCAn_nnnn'nTGG
Cuts at: 2462 3241 2462
Size: 779 5177
BstYI r'GATC_y
Cuts at: 1972 2573 2871 3201 3286 3653 4038 4055 5507
Size: 601 298 330 85 367 385 17 1452
Cuts at: 5507 5593 5605 5951 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 601 385 367 346 330 298
86 85 17 12
Bsu36I CC'TnA_GG
Cuts at: 2135 2291 2135
Size: 156 5800
BtgI C'CryG_G
Cuts at: 513 1065 2004 2481 2528 2667 2686 2722 513
Size: 552 939 477 47 139 19 36 3747
Fragments arranged by size:
3747 939 552 477 139 47 36 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 3472 4266 4286 2067
Size: 1405 794 20 3737
Fragments arranged by size:
3737 1405 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2023 2442 2464
Size: 54 1425 50 275 18 193 419 22
Cuts at: 2464 2525 2848 2911 3403 4292 5921 8
Size: 61 323 63 492 889 1629 43
Fragments arranged by size:
1629 1425 889 492 419 323 275 193
63 61 54 50 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3886 1353
Size: 2533 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2420 2779 2915 4019 4616 5403
Size: 393 397 391 359 136 1104 597 787
Cuts at: 5403 5671 1239
Size: 268 1524
Fragments arranged by size:
1524 1104 787 597 397 393 391 359
268 136
EcoO109I rG'GnC_Cy
Cuts at: 2076 2635 2746 2854 2076
Size: 559 111 108 5178
Fragments arranged by size:
5178 559 111 108
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2558 2558
Size: 5956
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 2337 2979 5103 1678
Size: 659 642 2124 2531
Fragments arranged by size:
2531 2124 659 642
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2565 2597 3077 3109 4597
Size: 32 135 32 993 32 480 32 1488
Cuts at: 4597 4629 4671 4703 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1488 993 480 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KasI G'GCGC_C
Cuts at: 2333 2333
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2271 5070 5254 1305
Size: 966 2799 184 2007
Fragments arranged by size:
2799 2007 966 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2913 10
Size: 54 1425 343 1081 3053
Fragments arranged by size:
3053 1425 1081 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2411 2565 3914 4273 4432 518
Size: 648 1245 154 1349 359 159 2042
Fragments arranged by size:
2042 1349 1245 648 359 159 154
MspA1I CmG'CkG
Cuts at: 1606 2155 2271 2698 2843 3063 3237 3372 4049
Size: 549 116 427 145 220 174 135 677
Cuts at: 4049 5197 5442 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 549 427 245 220 174
145 135 116
NaeI GCC'GGC
Cuts at: 2848 2848
Size: 5956
NarI GG'CG_CC
Cuts at: 2334 2334
Size: 5956
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4465
Fragments arranged by size:
4465 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 2846 2846
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3314 4859 1132
Size: 97 2085 1545 2229
Fragments arranged by size:
2229 2085 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2857 1108
Size: 1749 4207
PfoI T'CCnGG_A
Cuts at: 2619 2931 3192 2619
Size: 312 261 5383
Fragments arranged by size:
5383 312 261
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2076 2635 2746 2076
Size: 559 111 5286
Fragments arranged by size:
5286 559 111
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PspXI vC'TCGA_Gb
Cuts at: 2940 2940
Size: 5956
PstI C_TGCA'G
Cuts at: 830 2697 3308 830
Size: 1867 611 3478
Fragments arranged by size:
3478 1867 611
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2271 2698 2843 2271
Size: 427 145 5384
Fragments arranged by size:
5384 427 145
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SanDI GG'GwC_CC
Cuts at: 2076 2746 2076
Size: 670 5286
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 2906 4204 5916 2906
Size: 1298 1712 2946
Fragments arranged by size:
2946 1712 1298
SfcI C'TryA_G
Cuts at: 826 1045 2693 3304 4439 5120 5311 826
Size: 219 1648 611 1135 681 191 1471
Fragments arranged by size:
1648 1471 1135 681 611 219 191
SfoI GGC'GCC
Cuts at: 2335 2335
Size: 5956
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2940 4060 4961 5223 820
Size: 197 926 997 1120 901 262 1553
Fragments arranged by size:
1553 1120 997 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 2678 1517
Size: 1161 4795
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 3395 513
Size: 552 939 48 1343 3074
Fragments arranged by size:
3074 1343 939 552 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2402 2584 3983 4000 4153 4338 4783
Size: 1255 822 182 1399 17 153 185 445
Cuts at: 4783 325
Size: 1498
Fragments arranged by size:
1498 1399 1255 822 445 185 182 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2904 4202 5914
Size: 274 80 33 51 1443 927 1298 1712
Cuts at: 5914 96
Size: 138
Fragments arranged by size:
1712 1443 1298 927 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 5916 5948 2204
Size: 32 3680 32 2212
Fragments arranged by size:
3680 2212 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 2053
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 2940 1943
Size: 997 4959
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI
PsrI RsrII SacI SacII SapI SexAI SfiI SrfI
SwaI