Restriction map for
pFN21AE4390
(Circular) (Six-base) MAPSORT of: pFN21AE4390.seq Check: 1658 from: 1 to: 5941
pFN21AE4390 5941 bp
With 182 enzymes: SgfI *
March 28, 2012 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3026 4004 5388 5656 1632
Size: 1394 978 1384 268 1917
Fragments arranged by size:
1917 1394 1384 978 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 2184
Size: 32 5909
AlwNI CAG_nnn'CTG
Cuts at: 2498 2842 3103 3202 3684 5256 2498
Size: 344 261 99 482 1572 3183
Fragments arranged by size:
3183 1572 482 344 261 99
ApaI G_GGCC'C
Cuts at: 2039 2382 2039
Size: 343 5598
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 3250 3476 3628 5737 1493
Size: 1757 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2032 2108 2258 2514 3266 1870
Size: 73 82 7 76 150 256 752 4545
Fragments arranged by size:
4545 752 256 150 82 76 73 7
AvrII C'CTAG_G
Cuts at: 2060 2060
Size: 5941
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2908 3182 3262 4717 618
Size: 325 217 627 1121 274 80 1455 1842
Fragments arranged by size:
1842 1455 1121 627 325 274 217 80
BanII G_rGCy'C
Cuts at: 1391 2039 2053 2382 2617 2670 2711 3260 1391
Size: 648 14 329 235 53 41 549 4072
Fragments arranged by size:
4072 648 549 329 235 53 41 14
BbeI G_GCGC'C
Cuts at: 2912 2912
Size: 5941
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2694 928
Size: 971 795 4175
Fragments arranged by size:
4175 971 795
BbvCI CC'TCA_GC
Cuts at: 2465 2465
Size: 5941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2809 2843 2884 2918 4132 4166 1903
Size: 34 872 34 41 34 1214 34 3678
Fragments arranged by size:
3678 1214 872 41 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2576 2823 2837
Size: 107 122 71 631 605 904 247 14
Cuts at: 2837 2906 3053 3065 4552 136
Size: 69 147 12 1487 1525
Fragments arranged by size:
1525 1487 904 631 605 247 147 122
107 71 69 14 12
BglII A'GATC_T
Cuts at: 3171 5936 3171
Size: 2765 3176
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2259 2382 2977 3185 4002 5158 1790
Size: 249 220 123 595 208 817 1156 2573
Fragments arranged by size:
2573 1156 817 595 249 220 208 123
BmgBI CAC'GTC
Cuts at: 1318 2254 1318
Size: 936 5005
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 4630 442
Size: 416 53 83 399 24 2255 958 1753
Fragments arranged by size:
2255 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2909 2987 4601 1239
Size: 875 795 78 1614 2579
Fragments arranged by size:
2579 1614 875 795 78
Bpu10I CC'TnA_GC
Cuts at: 2211 2465 3224 2211
Size: 254 759 4928
Fragments arranged by size:
4928 759 254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4066 4931 5229 4066
Size: 865 298 4778
Fragments arranged by size:
4778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3121 3732 493
Size: 711 1917 611 2702
Fragments arranged by size:
2702 1917 711 611
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2909 3156 3748 4130
Size: 53 83 186 936 1376 247 592 382
Cuts at: 4130 4765 275
Size: 635 1451
Fragments arranged by size:
1451 1376 936 635 592 382 247 186
83 53
BsaWI w'CCGG_w
Cuts at: 2339 3234 4374 4772 5046 5193 5604 2339
Size: 895 1140 398 274 147 411 2676
Fragments arranged by size:
2676 1140 895 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5716
Fragments arranged by size:
5716 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2327 1186
Size: 1090 51 4800
Fragments arranged by size:
4800 1090 51
BseYI C'CCAG_C
Cuts at: 2203 2695 5144 5640 2203
Size: 492 2449 496 2504
Fragments arranged by size:
2504 2449 496 492
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5368
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3075 3391 4152 5180 1540
Size: 275 186 1074 316 761 1028 2301
Fragments arranged by size:
2301 1074 1028 761 316 275 186
BsiHKAI G_wGCw'C
Cuts at: 2053 4002 4087 5158 2053
Size: 1949 85 1071 2836
Fragments arranged by size:
2836 1949 1071 85
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2053 2259 2382 2617 2670 2711
Size: 399 249 14 206 123 235 53 41
Cuts at: 2711 2977 3185 3260 4002 4087 5158 1391
Size: 266 208 75 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 266 249 235 208
206 123 85 75 53 41 14
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 2349 3830 5560 2349
Size: 1481 1730 2730
Fragments arranged by size:
2730 1730 1481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 844
Size: 2452 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3142 3828 1394
Size: 429 1319 686 3507
Fragments arranged by size:
3507 1319 686 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2169 2847 4436 4610 65
Size: 1183 921 678 1589 174 1396
Fragments arranged by size:
1589 1396 1183 921 678 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3133 4585 4772 1700
Size: 110 1323 1452 187 2869
Fragments arranged by size:
2869 1452 1323 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2498 794
Size: 1704 4237
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstXI CCAn_nnnn'nTGG
Cuts at: 2308 2308
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 3099 3171 3271 3638 4023 4040 5492 5578
Size: 1127 72 100 367 385 17 1452 86
Cuts at: 5578 5590 5936 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1127 385 367 346 100 86
72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2247 2307 2323 2589 2715 3042
Size: 552 939 243 60 16 266 126 327
Cuts at: 3042 513
Size: 3412
Fragments arranged by size:
3412 939 552 327 266 243 126 60
16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2287 2812 487
Size: 683 56 1061 525 3616
Fragments arranged by size:
3616 1061 683 525 56
BtsI GCAGTG_nn'
Cuts at: 2491 3457 4251 4271 2491
Size: 966 794 20 4161
Fragments arranged by size:
4161 966 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2244 2718
Size: 54 1425 50 275 18 177 237 474
Cuts at: 2718 3045 3054 3388 4277 5906 8
Size: 327 9 334 889 1629 43
Fragments arranged by size:
1629 1425 889 474 334 327 275 237
177 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2204 3871 1353
Size: 851 1667 3423
Fragments arranged by size:
3423 1667 851
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2051 2051
Size: 5941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2909 2987 3026 4004 4601 5388
Size: 393 482 795 78 39 978 597 787
Cuts at: 5388 5656 1239
Size: 268 1524
Fragments arranged by size:
1524 978 795 787 597 482 393 268
78 39
EcoO109I rG'GnC_Cy
Cuts at: 2036 2064 2378 2839 2036
Size: 28 314 461 5138
Fragments arranged by size:
5138 461 314 28
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2912 5088 1678
Size: 1234 2176 2531
Fragments arranged by size:
2531 2176 1234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373
Size: 32 135 32 3010 32 42 32 2626
Fragments arranged by size:
3010 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2359 3285 3531 669
Size: 988 281 421 926 246 3079
Fragments arranged by size:
3079 988 926 421 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 2908 2908
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5055 5239 1305
Size: 3750 184 2007
Fragments arranged by size:
3750 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2246 3047 10
Size: 54 1425 343 414 801 2904
Fragments arranged by size:
2904 1425 801 414 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2252 2306 3899 4258 4417 518
Size: 648 1086 54 1593 359 159 2042
Fragments arranged by size:
2042 1593 1086 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2207 2325 3199 3357 4034 5182 5427 1606
Size: 601 118 874 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 874 677 601 245 158 118
NaeI GCC'GGC
Cuts at: 3135 3135
Size: 5941
NarI GG'CG_CC
Cuts at: 2909 2909
Size: 5941
NcoI C'CATG_G
Cuts at: 513 1065 2004 2247 2307 3042 513
Size: 552 939 243 60 735 3412
Fragments arranged by size:
3412 939 735 552 243 60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NgoMIV G'CCGG_C
Cuts at: 3133 3133
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 2139 3299 4844 1132
Size: 97 910 1160 1545 2229
Fragments arranged by size:
2229 1545 1160 910 97
PasI CC'CwG_GG
Cuts at: 1348 2842 1348
Size: 1494 4447
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2098 2556 2571 2663 2842 3103 3202 1108
Size: 990 458 15 92 179 261 99 3847
Fragments arranged by size:
3847 990 458 261 179 99 92 15
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2035 2378 2035
Size: 343 5598
PstI C_TGCA'G
Cuts at: 830 3293 830
Size: 2463 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
SacI G_AGCT'C
Cuts at: 2053 2053
Size: 5941
SacII CC_GC'GG
Cuts at: 2326 2326
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 3289 4424 5105 5296 826
Size: 219 2244 1135 681 191 1471
Fragments arranged by size:
2244 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3053 3053
Size: 5941
SfoI GGC'GCC
Cuts at: 2910 2910
Size: 5941
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2027 2516 3268 1872
Size: 155 489 752 4545
Fragments arranged by size:
4545 752 489 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4045 4946 5208 820
Size: 197 926 2102 901 262 1553
Fragments arranged by size:
2102 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SrfI GCCC'GGGC
Cuts at: 2027 2516 2027
Size: 489 5452
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2004 2060 2247 2307 2400 2751 3042
Size: 552 939 56 187 60 93 351 291
Cuts at: 3042 3380 513
Size: 338 3074
Fragments arranged by size:
3074 939 552 351 338 291 187 93
60 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3968 3985 4138 4323 4768 325
Size: 1255 2388 17 153 185 445 1498
Fragments arranged by size:
2388 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4187 5899 96
Size: 274 80 33 51 1443 2210 1712 138
Fragments arranged by size:
2210 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2470 2502 5901 5933 2470
Size: 32 3399 32 2478
Fragments arranged by size:
3399 2478 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 3049 3100 2803
Size: 246 51 5644
Fragments arranged by size:
5644 246 51
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2025 2514 3266 1870
Size: 155 489 752 4545
Fragments arranged by size:
4545 752 489 155
XmnI GAAnn'nnTTC
Cuts at: 3023 4070 3023
Size: 1047 4894
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SanDI SapI SexAI SwaI