Restriction map for
pFN21AE4389
(Circular) (Six-base) MAPSORT of: pFN21AE4389.seq Check: 5506 from: 1 to: 5929
pFN21AE4389 5929 bp
With 182 enzymes: SgfI *
March 28, 2012 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3739 278
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3272 1937
Size: 1335 4594
Acc65I G'GTAC_C
Cuts at: 3250 3250
Size: 5929
AclI AA'CG_TT
Cuts at: 1563 4056 4429 1563
Size: 2493 373 3063
Fragments arranged by size:
3063 2493 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2408 3992 5376 5644 1632
Size: 776 1584 1384 268 1917
Fragments arranged by size:
1917 1584 1384 776 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5732
AflIII A'CryG_T
Cuts at: 1203 4828 5805 1203
Size: 3625 977 1327
Fragments arranged by size:
3625 1327 977
AgeI A'CCGG_T
Cuts at: 2403 4760 2403
Size: 2357 3572
AhdI GACnn_n'nnGTC
Cuts at: 4658 4658
Size: 5929
AleI CACnn'nnGTG
Cuts at: 1166 2249 3110 1166
Size: 1083 861 3985
Fragments arranged by size:
3985 1083 861
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3128 1184
Size: 1944 3985
AlwNI CAG_nnn'CTG
Cuts at: 2062 2368 2713 3672 5244 2062
Size: 306 345 959 1572 2747
Fragments arranged by size:
2747 1572 959 345 306
ApaLI G'TGCA_C
Cuts at: 3986 5142 3986
Size: 1156 4773
ApoI r'AATT_y
Cuts at: 1493 3238 3464 3616 5725 1493
Size: 1745 226 152 2109 1697
Fragments arranged by size:
2109 1745 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4483 160
Size: 872 3451 1606
Fragments arranged by size:
3451 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 3254 1870
Size: 73 78 1233 4545
Fragments arranged by size:
4545 1233 78 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2050 2083 2050
Size: 33 5896
BamHI G'GATC_C
Cuts at: 3259 3626 3259
Size: 367 5562
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2167 2204 2254 2333 2836
Size: 325 217 627 380 37 50 79 503
Cuts at: 2836 2883 3250 4705 618
Size: 47 367 1455 1842
Fragments arranged by size:
1842 1455 627 503 380 367 325 217
79 50 47 37
BanII G_rGCy'C
Cuts at: 1391 3248 1391
Size: 1857 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2254 928
Size: 971 355 4603
Fragments arranged by size:
4603 971 355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4120 4154 1903
Size: 34 2183 34 3678
Fragments arranged by size:
3678 2183 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2078 3821 5031 2078
Size: 1743 1210 2976
Fragments arranged by size:
2976 1743 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5929
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2139 2507 4540
Size: 107 122 71 631 605 467 368 2033
Cuts at: 4540 136
Size: 1525
Fragments arranged by size:
2033 1525 631 605 467 368 122 107
71
BglII A'GATC_T
Cuts at: 5924 5924
Size: 5929
BlpI GC'TnA_GC
Cuts at: 3346 3346
Size: 5929
Bme1580I G_kGCm'C
Cuts at: 1790 2338 3990 5146 1790
Size: 548 1652 1156 2573
Fragments arranged by size:
2573 1652 1156 548
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5929
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2616 3213 3660
Size: 416 53 83 399 24 1199 597 447
Cuts at: 3660 4618 442
Size: 958 1753
Fragments arranged by size:
1753 1199 958 597 447 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3038 4589 1239
Size: 1799 1551 2579
Fragments arranged by size:
2579 1799 1551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4054 4919 5217 4054
Size: 865 298 4766
Fragments arranged by size:
4766 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4592 882
Size: 617 3093 2219
Fragments arranged by size:
3093 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3720 493
Size: 711 2516 2702
Fragments arranged by size:
2702 2516 711
BsaBI GATnn'nnATC
Cuts at: 5923 5923
Size: 5929
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3736 4118 4753 275
Size: 53 83 186 936 2203 382 635 1451
Fragments arranged by size:
2203 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2403 2839 4362 4760 5034 5181 5592 2403
Size: 436 1523 398 274 147 411 2740
Fragments arranged by size:
2740 1523 436 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2091 2121 1178
Size: 30 165 30 688 30 4986
Fragments arranged by size:
4986 688 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2042 2135 2212 2395 2459 2879 1186
Size: 853 3 93 77 183 64 420 4236
Fragments arranged by size:
4236 853 420 183 93 77 64 3
BseYI C'CCAG_C
Cuts at: 2137 3076 5132 5628 2137
Size: 939 2056 496 2438
Fragments arranged by size:
2438 2056 939 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2501 2645 3104 1163
Size: 573 765 144 459 3988
Fragments arranged by size:
3988 765 573 459 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3379 4140 5168 1540
Size: 275 186 1378 761 1028 2301
Fragments arranged by size:
2301 1378 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 3990 4075 5146 2034
Size: 1956 85 1071 2817
Fragments arranged by size:
2817 1956 1071 85
BsmI GAATG_Cn'
Cuts at: 2687 3440 3533 2687
Size: 753 93 5083
Fragments arranged by size:
5083 753 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2338 3248 3990 4075 5146 1391
Size: 399 244 304 910 742 85 1071 2174
Fragments arranged by size:
2174 1071 910 742 399 304 244 85
BspEI T'CCGG_A
Cuts at: 5592 5592
Size: 5929
BspHI T'CATG_A
Cuts at: 3818 5548 3818
Size: 1730 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 2809 3284 844
Size: 1226 739 475 3489
Fragments arranged by size:
3489 1226 739 475
BsrBI CCG'CTC
Cuts at: 1394 1823 2575 3816 1394
Size: 429 752 1241 3507
Fragments arranged by size:
3507 1241 752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4424 4598 65
Size: 1183 3176 174 1396
Fragments arranged by size:
3176 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2403 4573 4760 1700
Size: 110 593 2170 187 2869
Fragments arranged by size:
2869 2170 593 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5929
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5929
BssSI C'ACGA_G
Cuts at: 3989 5001 3989
Size: 1012 4917
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2516 2713 794
Size: 1722 197 4010
Fragments arranged by size:
4010 1722 197
BstBI TT'CG_AA
Cuts at: 4733 4733
Size: 5929
BstEII G'GTnAC_C
Cuts at: 1184 2182 2456 2596 2905 1184
Size: 998 274 140 309 4208
Fragments arranged by size:
4208 998 309 274 140
BstXI CCAn_nnnn'nTGG
Cuts at: 2894 2894
Size: 5929
BstYI r'GATC_y
Cuts at: 1972 3259 3626 4011 4028 5480 5566 5578 5924
Size: 1287 367 385 17 1452 86 12 346
Cuts at: 5924 1972
Size: 1977
Fragments arranged by size:
1977 1452 1287 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2504 2504
Size: 5929
BtgI C'CryG_G
Cuts at: 513 1065 3160 513
Size: 552 2095 3282
Fragments arranged by size:
3282 2095 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5190
Fragments arranged by size:
5190 683 56
BtsI GCAGTG_nn'
Cuts at: 3445 4239 4259 3445
Size: 794 20 5115
Fragments arranged by size:
5115 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5929
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5894
DraI TTT'AAA
Cuts at: 3233 3233
Size: 5929
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4936 809
Size: 507 3620 1802
Fragments arranged by size:
3620 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2198 3376 4265
Size: 54 1425 50 275 18 368 1178 889
Cuts at: 4265 5894 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1178 889 368 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3376 1537
Size: 275 1564 4090
Fragments arranged by size:
4090 1564 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 3859 1353
Size: 701 1805 3423
Fragments arranged by size:
3423 1805 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 4516 4890 5036 387
Size: 1064 138 453 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 453 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 3038 3992 4589 5376 5644 1239
Size: 393 776 630 954 597 787 268 1524
Fragments arranged by size:
1524 954 787 776 630 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2525 2735 2525
Size: 210 5719
EcoO109I rG'GnC_Cy
Cuts at: 2018 3073 2018
Size: 1055 4874
EcoRI G'AATT_C
Cuts at: 3238 3616 3238
Size: 378 5551
FspI TGC'GCA
Cuts at: 4435 4435
Size: 5929
HaeII r_GCGC'y
Cuts at: 1678 2817 5076 1678
Size: 1139 2259 2531
Fragments arranged by size:
2531 2259 1139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 4570 4602 4644
Size: 32 135 32 449 32 2517 32 42
Cuts at: 4644 4676 1373
Size: 32 2626
Fragments arranged by size:
2626 2517 449 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2193 3273 3519 669
Size: 988 281 255 1080 246 3079
Fragments arranged by size:
3079 1080 988 281 255 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5929
HpaI GTT'AAC
Cuts at: 3519 3519
Size: 5929
KpnI G_GTAC'C
Cuts at: 3254 3254
Size: 5929
MfeI C'AATT_G
Cuts at: 3528 3528
Size: 5929
MluI A'CGCG_T
Cuts at: 5805 5805
Size: 5929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5043 5227 1305
Size: 3738 184 2007
Fragments arranged by size:
3738 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2200 10
Size: 54 1425 343 368 3739
Fragments arranged by size:
3739 1425 368 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 2249 2423 3110 3887 4246 4405
Size: 648 1081 2 174 687 777 359 159
Cuts at: 4405 518
Size: 2042
Fragments arranged by size:
2042 1081 777 687 648 359 174 159
2
MspA1I CmG'CkG
Cuts at: 1606 2137 2485 2872 2894 3345 4022 5170 5415
Size: 531 348 387 22 451 677 1148 245
Cuts at: 5415 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 531 451 387 348 245
22
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5377
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5929
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5929
NotI GC'GGCC_GC
Cuts at: 3376 3376
Size: 5929
NspI r_CATG'y
Cuts at: 1132 1229 3287 4832 1132
Size: 97 2058 1545 2229
Fragments arranged by size:
2229 2058 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5929
PciI A'CATG_T
Cuts at: 4828 4828
Size: 5929
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2368 2467 1108
Size: 1260 99 4570
Fragments arranged by size:
4570 1260 99
PfoI T'CCnGG_A
Cuts at: 2691 2691
Size: 5929
PmeI GTTT'AAAC
Cuts at: 3233 3233
Size: 5929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 3998 4030 5511 5543 3137
Size: 32 829 32 1481 32 3523
Fragments arranged by size:
3523 1481 829 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2018 3073 2018
Size: 1055 4874
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5929
PsiI TTA'TAA
Cuts at: 3499 3499
Size: 5929
PstI C_TGCA'G
Cuts at: 830 2718 2877 3281 830
Size: 1888 159 404 3478
Fragments arranged by size:
3478 1888 404 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5929
PvuII CAG'CTG
Cuts at: 2137 2872 2894 2137
Size: 735 22 5172
Fragments arranged by size:
5172 735 22
SalI G'TCGA_C
Cuts at: 1936 3271 1936
Size: 1335 4594
SanDI GG'GwC_CC
Cuts at: 2018 3073 2018
Size: 1055 4874
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 5929
SbfI CC_TGCA'GG
Cuts at: 3281 3281
Size: 5929
ScaI AGT'ACT
Cuts at: 4177 5889 4177
Size: 1712 4217
SfcI C'TryA_G
Cuts at: 826 1045 2714 2873 3277 4412 5093 5284 826
Size: 219 1669 159 404 1135 681 191 1471
Fragments arranged by size:
1669 1471 1135 681 404 219 191 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5929
SmaI CCC'GGG
Cuts at: 1872 3256 1872
Size: 1384 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4033 4934 5196 820
Size: 197 926 2090 901 262 1553
Fragments arranged by size:
2090 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3720 493
Size: 3227 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5929
SphI G_CATG'C
Cuts at: 1132 3287 1132
Size: 2155 3774
SspI AAT'ATT
Cuts at: 5 52 3853 5
Size: 47 3801 2081
Fragments arranged by size:
3801 2081 47
StuI AGG'CCT
Cuts at: 1517 2297 1517
Size: 780 5149
StyI C'CwwG_G
Cuts at: 513 1065 2461 2969 3368 513
Size: 552 1396 508 399 3074
Fragments arranged by size:
3074 1396 552 508 399
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2049 2578 3109 3956 3973 4126 4311
Size: 1255 469 529 531 847 17 153 185
Cuts at: 4311 4756 325
Size: 445 1498
Fragments arranged by size:
1498 1255 847 531 529 469 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4175 5887 96
Size: 274 80 33 51 1443 2198 1712 138
Fragments arranged by size:
2198 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2091 2123 2333 2365 5889 5921 2091
Size: 32 210 32 3524 32 2099
Fragments arranged by size:
3524 2099 210 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5929
XbaI T'CTAG_A
Cuts at: 3265 3265
Size: 5929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5929
XmaI C'CCGG_G
Cuts at: 1870 3254 1870
Size: 1384 4545
XmnI GAAnn'nnTTC
Cuts at: 4058 4058
Size: 5929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3737 276
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI