Restriction map for
pFN21AE4386
(Circular) (Six-base) MAPSORT of: pFN21AE4386.seq Check: 5494 from: 1 to: 5923
pFN21AE4386 5923 bp
With 182 enzymes: SgfI *
March 28, 2012 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2415 3071 2415
Size: 656 5267
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3986 5370 5638 1632
Size: 2354 1384 268 1917
Fragments arranged by size:
2354 1917 1384 268
AfeI AGC'GCT
Cuts at: 2071 2071
Size: 5923
AflII C'TTAA_G
Cuts at: 820 1017 3125 820
Size: 197 2108 3618
Fragments arranged by size:
3618 2108 197
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 2057 2883 1166
Size: 891 826 4206
Fragments arranged by size:
4206 891 826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2934 1184
Size: 1750 4173
AlwNI CAG_nnn'CTG
Cuts at: 2167 2407 2422 2575 3666 5238 2167
Size: 240 15 153 1091 1572 2852
Fragments arranged by size:
2852 1572 1091 240 153 15
ApaLI G'TGCA_C
Cuts at: 2876 3980 5136 2876
Size: 1104 1156 3663
Fragments arranged by size:
3663 1156 1104
ApoI r'AATT_y
Cuts at: 1493 2412 3232 3458 3610 5719 1493
Size: 919 820 226 152 2109 1697
Fragments arranged by size:
2109 1697 919 820 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2501 3248 1870
Size: 73 558 747 4545
Fragments arranged by size:
4545 747 558 73
BamHI G'GATC_C
Cuts at: 2349 2752 3253 3620 2349
Size: 403 501 367 4652
Fragments arranged by size:
4652 501 403 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2217 2388 2691 2942 3059
Size: 325 217 627 430 171 303 251 117
Cuts at: 3059 3244 4699 618
Size: 185 1455 1842
Fragments arranged by size:
1842 1455 627 430 325 303 251 217
185 171 117
BanII G_rGCy'C
Cuts at: 1391 3242 1391
Size: 1851 4072
BbeI G_GCGC'C
Cuts at: 2221 2695 2946 2221
Size: 474 251 5198
Fragments arranged by size:
5198 474 251
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3152 928
Size: 971 1253 3699
Fragments arranged by size:
3699 1253 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 1903
Size: 34 2177 34 3678
Fragments arranged by size:
3678 2177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2436 2700 2738
Size: 107 122 71 631 605 764 264 38
Cuts at: 2738 2940 4534 136
Size: 202 1594 1525
Fragments arranged by size:
1594 1525 764 631 605 264 202 122
107 71 38
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 2201 3340 2201
Size: 1139 4784
Bme1580I G_kGCm'C
Cuts at: 1790 2880 3984 5140 1790
Size: 1090 1104 1156 2573
Fragments arranged by size:
2573 1156 1104 1090
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2851 3654 4612
Size: 416 53 83 399 24 1434 803 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1753 1434 958 803 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2031 3235 2031
Size: 1204 4719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2414 2801 3063 4583 1239
Size: 992 183 387 262 1520 2579
Fragments arranged by size:
2579 1520 992 387 262 183
Bpu10I CC'TnA_GC
Cuts at: 2073 2222 2073
Size: 149 5774
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2315 4048 4913 5211 2315
Size: 1733 865 298 3027
Fragments arranged by size:
3027 1733 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2634 4586 882
Size: 617 1135 1952 2219
Fragments arranged by size:
2219 1952 1135 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2218 2692 2943 3730
Size: 53 83 186 936 685 474 251 787
Cuts at: 3730 4112 4747 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 787 685 635 474 382 251
186 83 53
BsaWI w'CCGG_w
Cuts at: 4356 4754 5028 5175 5586 4356
Size: 398 274 147 411 4693
Fragments arranged by size:
4693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3083 3113 1178
Size: 30 165 30 1680 30 3988
Fragments arranged by size:
3988 1680 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5923
BseYI C'CCAG_C
Cuts at: 2376 2511 2773 2938 2960 5126 5622 2376
Size: 135 262 165 22 2166 496 2677
Fragments arranged by size:
2677 2166 496 262 165 135 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 2531 2849 2904 1163
Size: 573 372 423 318 55 4182
Fragments arranged by size:
4182 573 423 372 318 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 3373 4134 5162 1540
Size: 275 186 24 1348 761 1028 2301
Fragments arranged by size:
2301 1348 1028 761 275 186 24
BsiHKAI G_wGCw'C
Cuts at: 2017 2880 3984 4069 5140 2017
Size: 863 1104 85 1071 2800
Fragments arranged by size:
2800 1104 1071 863 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2880 3242 3984 4069 5140 1391
Size: 399 227 863 362 742 85 1071 2174
Fragments arranged by size:
2174 1071 863 742 399 362 227 85
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2415 3071 3278 844
Size: 1571 656 207 3489
Fragments arranged by size:
3489 1571 656 207
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 2215 3099 3810 1394
Size: 429 187 205 884 711 3507
Fragments arranged by size:
3507 884 711 429 205 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2550 2718 2930 4567 4754 1700
Size: 110 740 168 212 1637 187 2869
Fragments arranged by size:
2869 1637 740 212 187 168 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 2205 1925
Size: 280 5643
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2407 2554 794
Size: 1613 147 4163
Fragments arranged by size:
4163 1613 147
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2349 2752 3253 3620 4005 4022 5474 5560
Size: 377 403 501 367 385 17 1452 86
Cuts at: 5560 5572 5918 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 501 403 385 377 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2301 2622 2301
Size: 321 5602
BtgI C'CryG_G
Cuts at: 513 1065 2758 2925 3201 513
Size: 552 1693 167 276 3235
Fragments arranged by size:
3235 1693 552 276 167
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 2916 3170 3439 4233 4253 2916
Size: 254 269 794 20 4586
Fragments arranged by size:
4586 794 269 254 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DraIII CAC_nnn'GTG
Cuts at: 2371 2371
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2303 4930 809
Size: 507 987 2627 1802
Fragments arranged by size:
2627 1802 987 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2158 2472
Size: 54 1425 50 275 18 192 136 314
Cuts at: 2472 2823 2928 2932 3085 3370 4259 5888 8
Size: 351 105 4 153 285 889 1629 43
Fragments arranged by size:
1629 1425 889 351 314 285 275 192
153 136 105 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2022 3370 1537
Size: 275 210 1348 4090
Fragments arranged by size:
4090 1348 275 210
EarI CTCTTCn'nnn_
Cuts at: 1353 2366 3853 1353
Size: 1013 1487 3423
Fragments arranged by size:
3423 1487 1013
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2094 2227 2622 4510 4884 5030
Size: 1064 138 505 133 395 1888 374 146
Cuts at: 5030 387
Size: 1280
Fragments arranged by size:
1888 1280 1064 505 395 374 146 138
133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2414 2801 3063 3986 4583 5370
Size: 393 599 183 387 262 923 597 787
Cuts at: 5370 5638 1239
Size: 268 1524
Fragments arranged by size:
1524 923 787 599 597 393 387 268
262 183
EcoO109I rG'GnC_Cy
Cuts at: 2305 2305
Size: 5923
EcoRI G'AATT_C
Cuts at: 2412 3232 3610 2412
Size: 820 378 4725
Fragments arranged by size:
4725 820 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3154 3154
Size: 5923
FseI GG_CCGG'CC
Cuts at: 2934 2934
Size: 5923
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 2073 2221 2695 2946 5070 1678
Size: 395 148 474 251 2124 2531
Fragments arranged by size:
2531 2124 474 395 251 148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373
Size: 32 135 32 2992 32 42 32 2626
Fragments arranged by size:
2992 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2521 3267 3513 669
Size: 988 281 583 746 246 3079
Fragments arranged by size:
3079 988 746 583 281 246
HindIII A'AGCT_T
Cuts at: 748 3122 748
Size: 2374 3549
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KasI G'GCGC_C
Cuts at: 2217 2691 2942 2217
Size: 474 251 5198
Fragments arranged by size:
5198 474 251
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2042 3099 5037 5221 1305
Size: 737 1057 1938 184 2007
Fragments arranged by size:
2007 1938 1057 737 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2474 10
Size: 54 1425 343 642 3459
Fragments arranged by size:
3459 1425 642 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2883 3185 3881 4240 4399 518
Size: 648 891 826 302 696 359 159 2042
Fragments arranged by size:
2042 891 826 696 648 359 302 159
MspA1I CmG'CkG
Cuts at: 1606 2200 2386 2833 2938 2964 3115 3339 4016
Size: 594 186 447 105 26 151 224 677
Cuts at: 4016 5164 5409 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 594 447 245 224 186
151 105 26
NaeI GCC'GGC
Cuts at: 2932 2932
Size: 5923
NarI GG'CG_CC
Cuts at: 2218 2692 2943 2218
Size: 474 251 5198
Fragments arranged by size:
5198 474 251
NcoI C'CATG_G
Cuts at: 513 1065 3201 513
Size: 552 2136 3235
Fragments arranged by size:
3235 2136 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NgoMIV G'CCGG_C
Cuts at: 2930 2930
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 3281 4826 1132
Size: 97 2052 1545 2229
Fragments arranged by size:
2229 2052 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2559 2724 1348
Size: 1211 165 4547
Fragments arranged by size:
4547 1211 165
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2371 2470 3202 1108
Size: 1263 99 732 3829
Fragments arranged by size:
3829 1263 732 99
PfoI T'CCnGG_A
Cuts at: 2442 2442
Size: 5923
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2305 2305
Size: 5923
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PstI C_TGCA'G
Cuts at: 830 2154 2322 2868 2913 3068 3177 3275 830
Size: 1324 168 546 45 155 109 98 3478
Fragments arranged by size:
3478 1324 546 168 155 109 98 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2200 2833 2938 2200
Size: 633 105 5185
Fragments arranged by size:
5185 633 105
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 3092 4171 5883 3092
Size: 1079 1712 3132
Fragments arranged by size:
3132 1712 1079
SfcI C'TryA_G
Cuts at: 826 1045 2150 2318 2864 2902 2909 3064 3173
Size: 219 1105 168 546 38 7 155 109
Cuts at: 3173 3271 4406 5087 5278 826
Size: 98 1135 681 191 1471
Fragments arranged by size:
1471 1135 1105 681 546 219 191 168
155 109 98 38 7
SfoI GGC'GCC
Cuts at: 2219 2693 2944 2219
Size: 474 251 5198
Fragments arranged by size:
5198 474 251
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2330 3125 4027 4928 5190 820
Size: 197 926 387 795 902 901 262 1553
Fragments arranged by size:
1553 926 902 901 795 387 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 3201 3362 513
Size: 552 2136 161 3074
Fragments arranged by size:
3074 2136 552 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2873 2974 3950 3967 4120 4305 4750
Size: 1255 1293 101 976 17 153 185 445
Cuts at: 4750 325
Size: 1498
Fragments arranged by size:
1498 1293 1255 976 445 185 153 101
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3090 4169 5881
Size: 274 80 33 51 1443 1113 1079 1712
Cuts at: 5881 96
Size: 138
Fragments arranged by size:
1712 1443 1113 1079 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5883 5915 5883
Size: 32 5891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FspAI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI