Restriction map for
pFN21AE4385
(Circular) (Six-base) MAPSORT of: pFN21AE4385.seq Check: 3840 from: 1 to: 5923
pFN21AE4385 5923 bp
With 182 enzymes: SgfI *
March 28, 2012 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2541 2541
Size: 5923
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2787 3266 1937
Size: 850 479 4594
Fragments arranged by size:
4594 850 479
Acc65I G'GTAC_C
Cuts at: 2811 3244 2811
Size: 433 5490
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 2603 3986 5370 5638 1632
Size: 410 561 1383 1384 268 1917
Fragments arranged by size:
1917 1384 1383 561 410 268
AfeI AGC'GCT
Cuts at: 2729 2729
Size: 5923
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 3057 4822 5799 1203
Size: 1854 1765 977 1327
Fragments arranged by size:
1854 1765 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AlwNI CAG_nnn'CTG
Cuts at: 2040 3666 5238 2040
Size: 1626 1572 2725
Fragments arranged by size:
2725 1626 1572
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 2135 3133 3232 3458 3610 5719 1493
Size: 642 998 99 226 152 2109 1697
Fragments arranged by size:
2109 1697 998 642 226 152 99
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 3248 1870
Size: 73 1305 4545
Fragments arranged by size:
4545 1305 73
AvrII C'CTAG_G
Cuts at: 2324 2324
Size: 5923
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2644 2811 3104 3244 4699
Size: 325 217 627 857 167 293 140 1455
Cuts at: 4699 618
Size: 1842
Fragments arranged by size:
1842 1455 857 627 325 293 217 167
140
BanII G_rGCy'C
Cuts at: 1391 3242 1391
Size: 1851 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4952
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2590 2624 4114 4148 1903
Size: 34 653 34 1490 34 3678
Fragments arranged by size:
3678 1490 653 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2203 2464 3815 5025 2203
Size: 261 1351 1210 3101
Fragments arranged by size:
3101 1351 1210 261
BclI T'GATC_A
Cuts at: 1548 2614 1548
Size: 1066 4857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4534 136
Size: 107 122 71 631 605 2862 1525
Fragments arranged by size:
2862 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2337 5918 2337
Size: 3581 2342
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 2647 2678 3161 3984 5140 1790
Size: 857 31 483 823 1156 2573
Fragments arranged by size:
2573 1156 857 823 483 31
BmgBI CAC'GTC
Cuts at: 1318 3058 1318
Size: 1740 4183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 3654 4612
Size: 416 53 83 399 24 762 1475 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1753 1475 958 762 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4583 1239
Size: 3344 2579
Bpu10I CC'TnA_GC
Cuts at: 2354 2354
Size: 5923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2182 2876 4048 4913 5211 2182
Size: 694 1172 865 298 2894
Fragments arranged by size:
2894 1172 865 694 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3730 4112 4747 275
Size: 53 83 186 936 2197 382 635 1451
Fragments arranged by size:
2197 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2821 3207 4356 4754 5028 5175 5586 2821
Size: 386 1149 398 274 147 411 3158
Fragments arranged by size:
3158 1149 411 398 386 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5698
Fragments arranged by size:
5698 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5923
BseYI C'CCAG_C
Cuts at: 2537 5126 5622 2537
Size: 2589 496 2838
Fragments arranged by size:
2838 2589 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2433 2849 3079 1163
Size: 573 697 416 230 4007
Fragments arranged by size:
4007 697 573 416 230
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3984 4069 5140 3984
Size: 85 1071 4767
Fragments arranged by size:
4767 1071 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2647 2678 3161 3242 3984 4069 5140
Size: 399 857 31 483 81 742 85 1071
Cuts at: 5140 1391
Size: 2174
Fragments arranged by size:
2174 1071 857 742 483 399 85 81
31
BspEI T'CCGG_A
Cuts at: 2821 3207 5586 2821
Size: 386 2379 3158
Fragments arranged by size:
3158 2379 386
BspHI T'CATG_A
Cuts at: 2617 2994 3812 5542 2617
Size: 377 818 1730 2998
Fragments arranged by size:
2998 1730 818 377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2541 3186 3278 844
Size: 1697 645 92 3489
Fragments arranged by size:
3489 1697 645 92
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4567 4754 1700
Size: 110 2757 187 2869
Fragments arranged by size:
2869 2757 187 110
BsrGI T'GTAC_A
Cuts at: 96 2748 96
Size: 2652 3271
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 2713 1184
Size: 1529 4394
BstYI r'GATC_y
Cuts at: 1972 2337 2576 3253 3620 4005 4022 5474 5560
Size: 365 239 677 367 385 17 1452 86
Cuts at: 5560 5572 5918 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 677 385 367 365 346 239
86 17 12
BstZ17I GTA'TAC
Cuts at: 2788 2788
Size: 5923
Bsu36I CC'TnA_GG
Cuts at: 2630 2630
Size: 5923
BtgI C'CryG_G
Cuts at: 513 1065 2169 2457 513
Size: 552 1104 288 3979
Fragments arranged by size:
3979 1104 552 288
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 3194 3217 3439 4233 4253 3194
Size: 23 222 794 20 4864
Fragments arranged by size:
4864 794 222 23 20
ClaI AT'CG_AT
Cuts at: 1554 2610 1554
Size: 1056 4867
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 2119 3227 2119
Size: 1108 4815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 3370 4259
Size: 54 1425 50 275 18 336 1204 889
Cuts at: 4259 5888 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1204 889 336 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2311 2940 3853 1353
Size: 958 629 913 3423
Fragments arranged by size:
3423 958 913 629
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2603 3986 4583 5370 5638 1239
Size: 393 410 561 1383 597 787 268 1524
Fragments arranged by size:
1524 1383 787 597 561 410 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2258 2258
Size: 5923
EcoO109I rG'GnC_Cy
Cuts at: 2212 2419 2776 2212
Size: 207 357 5359
Fragments arranged by size:
5359 357 207
EcoRI G'AATT_C
Cuts at: 2135 3232 3610 2135
Size: 1097 378 4448
Fragments arranged by size:
4448 1097 378
EcoRV GAT'ATC
Cuts at: 2800 2800
Size: 5923
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 2731 5070 1678
Size: 1053 2339 2531
Fragments arranged by size:
2531 2339 1053
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2385 2417 2689
Size: 32 135 32 599 32 182 32 272
Cuts at: 2689 2721 4564 4596 4638 4670 1373
Size: 32 1843 32 42 32 2626
Fragments arranged by size:
2626 1843 599 272 182 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 2815 3248 2815
Size: 433 5490
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2686 5037 5221 1305
Size: 1381 2351 184 2007
Fragments arranged by size:
2351 2007 1381 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2168 10
Size: 54 1425 343 336 3765
Fragments arranged by size:
3765 1425 343 336 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2448 2993 2996 3881 4240 4399
Size: 648 909 373 545 3 885 359 159
Cuts at: 4399 518
Size: 2042
Fragments arranged by size:
2042 909 885 648 545 373 359 159
3
MspA1I CmG'CkG
Cuts at: 1606 2037 2573 3118 3339 4016 5164 5409 1606
Size: 431 536 545 221 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 545 536 431 245 221
NcoI C'CATG_G
Cuts at: 513 1065 2169 2457 513
Size: 552 1104 288 3979
Fragments arranged by size:
3979 1104 552 288
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 3281 4826 1132
Size: 97 2052 1545 2229
Fragments arranged by size:
2229 2052 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2972 1108
Size: 1864 4059
PfoI T'CCnGG_A
Cuts at: 2436 2436
Size: 5923
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 2776 2212
Size: 564 5359
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 2829 3493 2829
Size: 664 5259
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2037 2037
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SanDI GG'GwC_CC
Cuts at: 2776 2776
Size: 5923
SapI GCTCTTCn'nnn_
Cuts at: 2311 2311
Size: 5923
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SexAI A'CCwGG_T
Cuts at: 3012 3012
Size: 5923
SfcI C'TryA_G
Cuts at: 826 1045 3271 4406 5087 5278 826
Size: 219 2226 1135 681 191 1471
Fragments arranged by size:
2226 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2197 2891 4027 4928 5190 820
Size: 197 926 254 694 1136 901 262 1553
Fragments arranged by size:
1553 1136 926 901 694 262 254 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 2253 2276 1517
Size: 736 23 5164
Fragments arranged by size:
5164 736 23
StyI C'CwwG_G
Cuts at: 513 1065 2130 2169 2259 2324 2457 2514 2532
Size: 552 1065 39 90 65 133 57 18
Cuts at: 2532 2543 2906 3362 513
Size: 11 363 456 3074
Fragments arranged by size:
3074 1065 552 456 363 133 90 65
57 39 18 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2222 2662 3092 3950 3967 4120 4305
Size: 1255 642 440 430 858 17 153 185
Cuts at: 4305 4750 325
Size: 445 1498
Fragments arranged by size:
1498 1255 858 642 445 440 430 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2367 2748 4169
Size: 274 80 33 51 1443 390 381 1421
Cuts at: 4169 5881 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1421 390 381 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2461 2493 5883 5915 2461
Size: 32 3390 32 2469
Fragments arranged by size:
3390 2469 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2624 1316
Size: 1308 4615
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 2030 2729 2949 3080 4052 2030
Size: 699 220 131 972 3901
Fragments arranged by size:
3901 972 699 220 131
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI DraIII EcoICRI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI
SrfI SwaI XcmI