Restriction map for
pFN21AE4382
(Circular) (Six-base) MAPSORT of: pFN21AE4382.seq Check: 1869 from: 1 to: 5914
pFN21AE4382 5914 bp
With 182 enzymes: SgfI *
March 28, 2012 14:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2829 3724 278
Size: 53 83 186 936 1293 895 2468
Fragments arranged by size:
2468 1293 936 895 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3257 1937
Size: 1320 4594
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3129 3188 3977 5361 5629 1632
Size: 1497 59 789 1384 268 1917
Fragments arranged by size:
1917 1497 1384 789 268 59
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 4813 5790 1203
Size: 3610 977 1327
Fragments arranged by size:
3610 1327 977
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 2224 4643 2224
Size: 2419 3495
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5914
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2441 2473 2441
Size: 32 5882
AlwNI CAG_nnn'CTG
Cuts at: 3657 5229 3657
Size: 1572 4342
ApaLI G'TGCA_C
Cuts at: 3971 5127 3971
Size: 1156 4758
ApoI r'AATT_y
Cuts at: 1493 3160 3223 3449 3601 5710 1493
Size: 1667 63 226 152 2109 1697
Fragments arranged by size:
2109 1697 1667 226 152 63
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 3239 1870
Size: 73 1296 4545
Fragments arranged by size:
4545 1296 73
BamHI G'GATC_C
Cuts at: 2416 3244 3611 2416
Size: 828 367 4719
Fragments arranged by size:
4719 828 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3235 4690 618
Size: 325 217 627 1448 1455 1842
Fragments arranged by size:
1842 1455 1448 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3233 1391
Size: 1842 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2772 3117 3176 928
Size: 971 873 345 59 3666
Fragments arranged by size:
3666 971 873 345 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4105 4139 1903
Size: 34 2168 34 3678
Fragments arranged by size:
3678 2168 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 2206 1548
Size: 658 5256
BfrBI ATG'CAT
Cuts at: 2259 2259
Size: 5914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4525 136
Size: 107 122 71 631 605 2853 1525
Fragments arranged by size:
2853 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5909 5909
Size: 5914
BlpI GC'TnA_GC
Cuts at: 2198 3331 2198
Size: 1133 4781
Bme1580I G_kGCm'C
Cuts at: 1790 3975 5131 1790
Size: 2185 1156 2573
Fragments arranged by size:
2573 2185 1156
BmgBI CAC'GTC
Cuts at: 1318 2243 1318
Size: 925 4989
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3645 4603 442
Size: 416 53 83 399 24 2228 958 1753
Fragments arranged by size:
2228 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2864 3162 4574 1239
Size: 1625 298 1412 2579
Fragments arranged by size:
2579 1625 1412 298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4039 4904 5202 4039
Size: 865 298 4751
Fragments arranged by size:
4751 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2506 3028 4577 882
Size: 617 1007 522 1549 2219
Fragments arranged by size:
2219 1549 1007 617 522
BsaAI yAC'GTr
Cuts at: 493 1204 3705 493
Size: 711 2501 2702
Fragments arranged by size:
2702 2501 711
BsaBI GATnn'nnATC
Cuts at: 5908 5908
Size: 5914
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2826 3003 3721 4103
Size: 53 83 186 936 1293 177 718 382
Cuts at: 4103 4738 275
Size: 635 1451
Fragments arranged by size:
1451 1293 936 718 635 382 186 177
83 53
BsaWI w'CCGG_w
Cuts at: 4347 4745 5019 5166 5577 4347
Size: 398 274 147 411 4684
Fragments arranged by size:
4684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5689
Fragments arranged by size:
5689 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3046 1186
Size: 1860 4054
BseYI C'CCAG_C
Cuts at: 2727 2833 5117 5613 2727
Size: 106 2284 496 3028
Fragments arranged by size:
3028 2284 496 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2814 1163
Size: 573 1078 4263
Fragments arranged by size:
4263 1078 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3364 4125 5153 1540
Size: 275 186 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2930 3975 4060 5131 2930
Size: 1045 85 1071 3713
Fragments arranged by size:
3713 1071 1045 85
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2930 3233 3975 4060 5131 1391
Size: 399 1140 303 742 85 1071 2174
Fragments arranged by size:
2174 1140 1071 742 399 303 85
BspEI T'CCGG_A
Cuts at: 5577 5577
Size: 5914
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3269 844
Size: 2425 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3801 1394
Size: 429 1978 3507
Fragments arranged by size:
3507 1978 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 2571 4409 4583 65
Size: 1183 879 444 1838 174 1396
Fragments arranged by size:
1838 1396 1183 879 444 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4558 4745 1700
Size: 110 2748 187 2869
Fragments arranged by size:
2869 2748 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5914
BssSI C'ACGA_G
Cuts at: 3974 4986 3974
Size: 1012 4902
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5914
BstBI TT'CG_AA
Cuts at: 4718 4718
Size: 5914
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5914
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2356
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 2416 3244 3611 3996 4013 5465 5551 5563
Size: 444 828 367 385 17 1452 86 12
Cuts at: 5563 5909 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 828 444 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3029 3029
Size: 5914
BtgI C'CryG_G
Cuts at: 513 1065 2004 2265 513
Size: 552 939 261 4162
Fragments arranged by size:
4162 939 552 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5175
Fragments arranged by size:
5175 683 56
BtsI GCAGTG_nn'
Cuts at: 2797 3430 4224 4244 2797
Size: 633 794 20 4467
Fragments arranged by size:
4467 794 633 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 3218 3218
Size: 5914
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4921 809
Size: 507 3605 1802
Fragments arranged by size:
3605 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 3361 4250
Size: 54 1425 50 275 18 331 1200 889
Cuts at: 4250 5879 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1200 889 331 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3361 1537
Size: 275 1549 4090
Fragments arranged by size:
4090 1549 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3844 1353
Size: 2491 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4501 4875 5021 387
Size: 1064 138 2912 374 146 1280
Fragments arranged by size:
2912 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2864 3129 3162 3188 3977 4574 5361
Size: 393 1232 265 33 26 789 597 787
Cuts at: 5361 5629 1239
Size: 268 1524
Fragments arranged by size:
1524 1232 789 787 597 393 268 265
33 26
EcoNI CCTnn'n_nnAGG
Cuts at: 3203 3203
Size: 5914
EcoRI G'AATT_C
Cuts at: 3223 3601 3223
Size: 378 5536
FspI TGC'GCA
Cuts at: 4420 4420
Size: 5914
HaeII r_GCGC'y
Cuts at: 1678 2236 2603 5061 1678
Size: 558 367 2458 2531
Fragments arranged by size:
2531 2458 558 367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 3025 3057 4555
Size: 32 135 32 638 32 783 32 1498
Cuts at: 4555 4587 4629 4661 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1498 783 638 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3504 669
Size: 988 281 1320 246 3079
Fragments arranged by size:
3079 1320 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2994 748
Size: 2246 3668
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 3513 3513
Size: 5914
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2586 5028 5212 1305
Size: 1281 2442 184 2007
Fragments arranged by size:
2442 2007 1281 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2163 10
Size: 54 1425 343 331 3761
Fragments arranged by size:
3761 1425 343 331 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2055 2264 2275 2295 2661 3872 4231
Size: 648 889 209 11 20 366 1211 359
Cuts at: 4231 4390 518
Size: 159 2042
Fragments arranged by size:
2042 1211 889 648 366 359 209 159
20 11
MspA1I CmG'CkG
Cuts at: 1606 2525 3330 4007 5155 5400 1606
Size: 919 805 677 1148 245 2120
Fragments arranged by size:
2120 1148 919 805 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2265 513
Size: 552 939 261 4162
Fragments arranged by size:
4162 939 552 261
NdeI CA'TA_TG
Cuts at: 387 2272 387
Size: 1885 4029
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5914
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NsiI A_TGCA'T
Cuts at: 2261 2261
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 2197 2867 3272 4817 1132
Size: 97 968 670 405 1545 2229
Fragments arranged by size:
2229 1545 968 670 405 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5914
PciI A'CATG_T
Cuts at: 4813 4813
Size: 5914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5914
PfoI T'CCnGG_A
Cuts at: 2027 2880 2027
Size: 853 5061
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 2112 3484 2112
Size: 1372 4542
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 5882
PstI C_TGCA'G
Cuts at: 830 2483 3266 830
Size: 1653 783 3478
Fragments arranged by size:
3478 1653 783
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
PvuII CAG'CTG
Cuts at: 2525 2525
Size: 5914
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SbfI CC_TGCA'GG
Cuts at: 3266 3266
Size: 5914
ScaI AGT'ACT
Cuts at: 4162 5874 4162
Size: 1712 4202
SfcI C'TryA_G
Cuts at: 826 1045 2479 3262 4397 5078 5269 826
Size: 219 1434 783 1135 681 191 1471
Fragments arranged by size:
1471 1434 1135 783 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 3241 1872
Size: 1369 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4018 4919 5181 820
Size: 197 926 2075 901 262 1553
Fragments arranged by size:
2075 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 3272 1132
Size: 2140 3774
SspI AAT'ATT
Cuts at: 5 52 2323 3838 5
Size: 47 2271 1515 2081
Fragments arranged by size:
2271 2081 1515 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5914
StyI C'CwwG_G
Cuts at: 513 1065 2004 2265 3353 513
Size: 552 939 261 1088 3074
Fragments arranged by size:
3074 1088 939 552 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3941 3958 4111 4296 4741 325
Size: 1255 2361 17 153 185 445 1498
Fragments arranged by size:
2361 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3060 3182 4160
Size: 274 80 33 51 1443 1083 122 978
Cuts at: 4160 5872 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1083 978 274 138 122 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5874 5906 5874
Size: 32 5882
Tth111I GACn'n_nGTC
Cuts at: 1316 2390 1316
Size: 1074 4840
XbaI T'CTAG_A
Cuts at: 3250 3250
Size: 5914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2849 2849
Size: 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5914
XmaI C'CCGG_G
Cuts at: 1870 3239 1870
Size: 1369 4545
XmnI GAAnn'nnTTC
Cuts at: 4043 4043
Size: 5914
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2827 3722 276
Size: 53 83 186 936 1293 895 2468
Fragments arranged by size:
2468 1293 936 895 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI