Restriction map for
pFN21AE4381
(Circular) (Six-base) MAPSORT of: pFN21AE4381.seq Check: 2844 from: 1 to: 5908
pFN21AE4381 5908 bp
With 182 enzymes: SgfI *
March 28, 2012 14:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3718 278
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3251 1937
Size: 1314 4594
Acc65I G'GTAC_C
Cuts at: 3229 3229
Size: 5908
AclI AA'CG_TT
Cuts at: 1563 4035 4408 1563
Size: 2472 373 3063
Fragments arranged by size:
3063 2472 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2412 2927 3971 5355 5623 1632
Size: 780 515 1044 1384 268 1917
Fragments arranged by size:
1917 1384 1044 780 515 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5711
AflIII A'CryG_T
Cuts at: 1203 2629 3142 4807 5784 1203
Size: 1426 513 1665 977 1327
Fragments arranged by size:
1665 1426 1327 977 513
AgeI A'CCGG_T
Cuts at: 2734 4739 2734
Size: 2005 3903
AhdI GACnn_n'nnGTC
Cuts at: 4637 4637
Size: 5908
AleI CACnn'nnGTG
Cuts at: 1166 2908 1166
Size: 1742 4166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5908
AlwNI CAG_nnn'CTG
Cuts at: 2545 3651 5223 2545
Size: 1106 1572 3230
Fragments arranged by size:
3230 1572 1106
ApaLI G'TGCA_C
Cuts at: 3965 5121 3965
Size: 1156 4752
ApoI r'AATT_y
Cuts at: 1493 2120 2295 3217 3443 3595 5704 1493
Size: 627 175 922 226 152 2109 1697
Fragments arranged by size:
2109 1697 922 627 226 175 152
AseI AT'TA_AT
Cuts at: 160 1032 4462 160
Size: 872 3430 1606
Fragments arranged by size:
3430 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 2112 2552 2668 3233 1870
Size: 73 169 440 116 565 4545
Fragments arranged by size:
4545 565 440 169 116 73
BamHI G'GATC_C
Cuts at: 3238 3605 3238
Size: 367 5541
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2663 2881 3036 3110 3229
Size: 325 217 627 876 218 155 74 119
Cuts at: 3229 4684 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 876 627 325 218 217 155
119 74
BanII G_rGCy'C
Cuts at: 1391 2084 2534 2802 3227 1391
Size: 693 450 268 425 4072
Fragments arranged by size:
4072 693 450 425 268
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4937
BbvCI CC'TCA_GC
Cuts at: 2169 3018 2169
Size: 849 5059
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2357 2391 4099 4133 1903
Size: 34 50 34 336 34 1708 34 3678
Fragments arranged by size:
3678 1708 336 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3800 5010 3800
Size: 1210 4698
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4519 136
Size: 107 122 71 631 605 2847 1525
Fragments arranged by size:
2847 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5903 5903
Size: 5908
BlpI GC'TnA_GC
Cuts at: 3325 3325
Size: 5908
Bme1580I G_kGCm'C
Cuts at: 1790 2666 3969 5125 1790
Size: 876 1303 1156 2573
Fragments arranged by size:
2573 1303 1156 876
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2476 2668 3639
Size: 416 53 83 399 24 1059 192 971
Cuts at: 3639 4597 442
Size: 958 1753
Fragments arranged by size:
1753 1059 971 958 416 399 192 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3026 1056
Size: 1970 3938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2449 2522 4568 1239
Size: 1210 73 2046 2579
Fragments arranged by size:
2579 2046 1210 73
Bpu10I CC'TnA_GC
Cuts at: 2169 3018 2169
Size: 849 5059
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4033 4898 5196 4033
Size: 865 298 4745
Fragments arranged by size:
4745 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2841 4571 882
Size: 617 1342 1730 2219
Fragments arranged by size:
2219 1730 1342 617
BsaAI yAC'GTr
Cuts at: 493 1204 3699 493
Size: 711 2495 2702
Fragments arranged by size:
2702 2495 711
BsaBI GATnn'nnATC
Cuts at: 2327 5902 2327
Size: 3575 2333
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3715 4097 4732 275
Size: 53 83 186 936 2182 382 635 1451
Fragments arranged by size:
2182 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2584 2734 4341 4739 5013 5160 5571 2584
Size: 150 1607 398 274 147 411 2921
Fragments arranged by size:
2921 1607 411 398 274 150 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2425 2455 2821 2851 1178
Size: 30 165 30 1022 30 366 30 4235
Fragments arranged by size:
4235 1022 366 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2446 1186
Size: 1260 4648
BseYI C'CCAG_C
Cuts at: 5111 5607 5111
Size: 496 5412
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2008 1163
Size: 573 272 5063
Fragments arranged by size:
5063 573 272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2133 2585 3358 4119 5147 1540
Size: 275 186 132 452 773 761 1028 2301
Fragments arranged by size:
2301 1028 773 761 452 275 186 132
BsiHKAI G_wGCw'C
Cuts at: 2034 2084 2802 3069 3969 4054 5125 2034
Size: 50 718 267 900 85 1071 2817
Fragments arranged by size:
2817 1071 900 718 267 85 50
BsmI GAATG_Cn'
Cuts at: 3419 3512 3419
Size: 93 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2084 2534 2666 2802 3069 3227
Size: 399 244 50 450 132 136 267 158
Cuts at: 3227 3969 4054 5125 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 399 267 244 158
136 132 85 50
BspEI T'CCGG_A
Cuts at: 5571 5571
Size: 5908
BspHI T'CATG_A
Cuts at: 3797 5527 3797
Size: 1730 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3263 844
Size: 2419 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3795 1394
Size: 429 1972 3507
Fragments arranged by size:
3507 1972 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2832 4403 4577 65
Size: 1183 1584 1571 174 1396
Fragments arranged by size:
1584 1571 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2734 4552 4739 1700
Size: 110 924 1818 187 2869
Fragments arranged by size:
2869 1818 924 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5908
BssHII G'CGCG_C
Cuts at: 1925 2144 1925
Size: 219 5689
BssSI C'ACGA_G
Cuts at: 3968 4980 3968
Size: 1012 4896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5908
BstBI TT'CG_AA
Cuts at: 4712 4712
Size: 5908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5908
BstYI r'GATC_y
Cuts at: 1972 3238 3605 3990 4007 5459 5545 5557 5903
Size: 1266 367 385 17 1452 86 12 346
Cuts at: 5903 1972
Size: 1977
Fragments arranged by size:
1977 1452 1266 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2601 513
Size: 552 1536 3820
Fragments arranged by size:
3820 1536 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2125 2314 2666 487
Size: 683 56 899 189 352 3729
Fragments arranged by size:
3729 899 683 352 189 56
BtsI GCAGTG_nn'
Cuts at: 3424 4218 4238 3424
Size: 794 20 5094
Fragments arranged by size:
5094 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 3212 3212
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4915 809
Size: 507 3599 1802
Fragments arranged by size:
3599 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3355 4244 5873
Size: 54 1425 50 275 18 1525 889 1629
Cuts at: 5873 8
Size: 43
Fragments arranged by size:
1629 1525 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3355 1537
Size: 275 1543 4090
Fragments arranged by size:
4090 1543 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 3838 1353
Size: 683 1802 3423
Fragments arranged by size:
3423 1802 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4495 4869 5015 387
Size: 1064 138 2906 374 146 1280
Fragments arranged by size:
2906 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2082 2800 2082
Size: 718 5190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2412 2449 2522 2927 3971 4568 5355
Size: 393 780 37 73 405 1044 597 787
Cuts at: 5355 5623 1239
Size: 268 1524
Fragments arranged by size:
1524 1044 787 780 597 405 393 268
73 37
EcoRI G'AATT_C
Cuts at: 3217 3595 3217
Size: 378 5530
FspI TGC'GCA
Cuts at: 3173 4414 3173
Size: 1241 4667
HaeII r_GCGC'y
Cuts at: 1678 5055 1678
Size: 3377 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2425 2457 2821 2853 3188
Size: 32 135 32 853 32 364 32 335
Cuts at: 3188 3220 4549 4581 4623 4655 1373
Size: 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1329 853 364 335 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2350 3252 3498 669
Size: 988 281 412 902 246 3079
Fragments arranged by size:
3079 988 902 412 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5908
HpaI GTT'AAC
Cuts at: 2350 3498 2350
Size: 1148 4760
KpnI G_GTAC'C
Cuts at: 3233 3233
Size: 5908
MfeI C'AATT_G
Cuts at: 2988 3507 2988
Size: 519 5389
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2027 5022 5206 1305
Size: 722 2995 184 2007
Fragments arranged by size:
2995 2007 722 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4086
Fragments arranged by size:
4086 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 2651 2908 3866 4225 4384 518
Size: 648 1434 51 257 958 359 159 2042
Fragments arranged by size:
2042 1434 958 648 359 257 159 51
MspA1I CmG'CkG
Cuts at: 1606 2791 3324 4001 5149 5394 1606
Size: 1185 533 677 1148 245 2120
Fragments arranged by size:
2120 1185 1148 677 533 245
NcoI C'CATG_G
Cuts at: 513 1065 2601 513
Size: 552 1536 3820
Fragments arranged by size:
3820 1536 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5908
NheI G'CTAG_C
Cuts at: 1052 3022 1052
Size: 1970 3938
NotI GC'GGCC_GC
Cuts at: 3355 3355
Size: 5908
NspI r_CATG'y
Cuts at: 1132 1229 2633 3146 3266 4811 1132
Size: 97 1404 513 120 1545 2229
Fragments arranged by size:
2229 1545 1404 513 120 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5908
PciI A'CATG_T
Cuts at: 2629 3142 4807 2629
Size: 513 1665 3730
Fragments arranged by size:
3730 1665 513
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 1108
Size: 1539 4369
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 5490 5522 3977
Size: 32 1481 32 4363
Fragments arranged by size:
4363 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5908
PsiI TTA'TAA
Cuts at: 3478 3478
Size: 5908
PspXI vC'TCGA_Gb
Cuts at: 2552 2552
Size: 5908
PstI C_TGCA'G
Cuts at: 830 2550 3260 830
Size: 1720 710 3478
Fragments arranged by size:
3478 1720 710
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5908
PvuII CAG'CTG
Cuts at: 2791 2791
Size: 5908
SacI G_AGCT'C
Cuts at: 2084 2802 2084
Size: 718 5190
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SbfI CC_TGCA'GG
Cuts at: 3260 3260
Size: 5908
ScaI AGT'ACT
Cuts at: 4156 5868 4156
Size: 1712 4196
SfcI C'TryA_G
Cuts at: 826 1045 2546 2706 3184 3199 3256 4391 5072
Size: 219 1501 160 478 15 57 1135 681
Cuts at: 5072 5263 826
Size: 191 1471
Fragments arranged by size:
1501 1471 1135 681 478 219 191 160
57 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5908
SmaI CCC'GGG
Cuts at: 1872 2114 3235 1872
Size: 242 1121 4545
Fragments arranged by size:
4545 1121 242
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 2668 4012 4913 5175 820
Size: 197 926 609 116 1344 901 262 1553
Fragments arranged by size:
1553 1344 926 901 609 262 197 116
SnaBI TAC'GTA
Cuts at: 493 3699 493
Size: 3206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 3266 1132
Size: 2134 3774
SrfI GCCC'GGGC
Cuts at: 2114 2114
Size: 5908
SspI AAT'ATT
Cuts at: 5 52 3832 5
Size: 47 3780 2081
Fragments arranged by size:
3780 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5908
StyI C'CwwG_G
Cuts at: 513 1065 2601 2641 3106 3347 513
Size: 552 1536 40 465 241 3074
Fragments arranged by size:
3074 1536 552 465 241 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2076 2266 3935 3952 4105 4290 4735
Size: 1255 496 190 1669 17 153 185 445
Cuts at: 4735 325
Size: 1498
Fragments arranged by size:
1669 1498 1255 496 445 190 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 4154 5866
Size: 274 80 33 51 1443 678 1499 1712
Cuts at: 5866 96
Size: 138
Fragments arranged by size:
1712 1499 1443 678 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2262 2294 2865 2897 5868 5900 2262
Size: 32 571 32 2971 32 2270
Fragments arranged by size:
2971 2270 571 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2833 1316
Size: 1517 4391
XbaI T'CTAG_A
Cuts at: 3244 3244
Size: 5908
XhoI C'TCGA_G
Cuts at: 1943 2552 2668 1943
Size: 609 116 5183
Fragments arranged by size:
5183 609 116
XmaI C'CCGG_G
Cuts at: 1870 2112 3233 1870
Size: 242 1121 4545
Fragments arranged by size:
4545 1121 242
XmnI GAAnn'nnTTC
Cuts at: 2397 4037 2397
Size: 1640 4268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3716 276
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SwaI XcmI