Restriction map for
pFN21AE4380
(Circular) (Six-base) MAPSORT of: pFN21AE4380.seq Check: 8744 from: 1 to: 5905
pFN21AE4380 5905 bp
With 182 enzymes: SgfI *
March 28, 2012 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2824 2824
Size: 5905
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2216 3968 5352 5620 1632
Size: 584 1752 1384 268 1917
Fragments arranged by size:
1917 1752 1384 584 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 2657 1166
Size: 1491 4414
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3021 1184
Size: 1837 4068
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2438 2470 2438
Size: 32 5873
AlwNI CAG_nnn'CTG
Cuts at: 3034 3080 3648 5220 3034
Size: 46 568 1572 3719
Fragments arranged by size:
3719 1572 568 46
ApaI G_GGCC'C
Cuts at: 2954 2954
Size: 5905
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 3166 3214 3440 3592 5701 1493
Size: 1673 48 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 226 152 48
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 1870
Size: 73 1287 4545
Fragments arranged by size:
4545 1287 73
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 2659 3226 4681 618
Size: 325 217 627 396 476 567 1455 1842
Fragments arranged by size:
1842 1455 627 567 476 396 325 217
BanII G_rGCy'C
Cuts at: 1391 2111 2954 3224 1391
Size: 720 843 270 4072
Fragments arranged by size:
4072 843 720 270
BbeI G_GCGC'C
Cuts at: 2187 2187
Size: 5905
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2850 928
Size: 971 951 3983
Fragments arranged by size:
3983 971 951
BbvCI CC'TCA_GC
Cuts at: 2619 2619
Size: 5905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 3175 1548
Size: 1627 4278
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 4516 136
Size: 107 122 71 631 605 394 2450 1525
Fragments arranged by size:
2450 1525 631 605 394 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 2954 3966 5122 1790
Size: 1164 1012 1156 2573
Fragments arranged by size:
2573 1164 1156 1012
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2709 2897 3636
Size: 416 53 83 399 24 1292 188 739
Cuts at: 3636 4594 442
Size: 958 1753
Fragments arranged by size:
1753 1292 958 739 416 399 188 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2243 2297 2402 2465 4565 1239
Size: 872 132 54 105 63 2100 2579
Fragments arranged by size:
2579 2100 872 132 105 63 54
Bpu10I CC'TnA_GC
Cuts at: 2468 2619 2955 2468
Size: 151 336 5418
Fragments arranged by size:
5418 336 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2559 2955 4568 882
Size: 617 1060 396 1613 2219
Fragments arranged by size:
2219 1613 1060 617 396
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2023 2184 3712 4094
Size: 53 83 186 936 490 161 1528 382
Cuts at: 4094 4729 275
Size: 635 1451
Fragments arranged by size:
1528 1451 936 635 490 382 186 161
83 53
BsaWI w'CCGG_w
Cuts at: 4338 4736 5010 5157 5568 4338
Size: 398 274 147 411 4675
Fragments arranged by size:
4675 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 2583 2613 1178
Size: 30 165 30 1035 30 115 30 4470
Fragments arranged by size:
4470 1035 165 115 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2528 2804 3005 3008 3054 1186
Size: 1342 276 201 3 46 4037
Fragments arranged by size:
4037 1342 276 201 46 3
BseYI C'CCAG_C
Cuts at: 5108 5604 5108
Size: 496 5409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2927 1163
Size: 573 1191 4141
Fragments arranged by size:
4141 1191 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2111 3966 4051 5122 2111
Size: 1855 85 1071 2894
Fragments arranged by size:
2894 1855 1071 85
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 2954 3224 3966 4051 5122 1391
Size: 399 321 843 270 742 85 1071 2174
Fragments arranged by size:
2174 1071 843 742 399 321 270 85
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2824 3260 844
Size: 1980 436 3489
Fragments arranged by size:
3489 1980 436
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4400 4574 65
Size: 1183 3152 174 1396
Fragments arranged by size:
3152 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4549 4736 1700
Size: 110 2739 187 2869
Fragments arranged by size:
2869 2739 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 2048 3965 4977 2048
Size: 1917 1012 2976
Fragments arranged by size:
2976 1917 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 2530 1184
Size: 1346 4559
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2772 3235 3602 3987 4004 5456 5542 5554
Size: 800 463 367 385 17 1452 86 12
Cuts at: 5554 5900 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 800 463 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2187 513
Size: 552 939 183 4231
Fragments arranged by size:
4231 939 552 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 3421 4215 4235 3421
Size: 794 20 5091
Fragments arranged by size:
5091 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2969 4912 809
Size: 507 1653 1943 1802
Fragments arranged by size:
1943 1802 1653 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 3352 4241
Size: 54 1425 50 275 18 225 1297 889
Cuts at: 4241 5870 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1297 889 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2520 2857 3835 1353
Size: 1167 337 978 3423
Fragments arranged by size:
3423 1167 978 337
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4492 4866 5012 387
Size: 1064 138 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2109 2109
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2216 2243 2297 2402 2465 3968
Size: 393 479 105 27 54 105 63 1503
Cuts at: 3968 4565 5352 5620 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1503 787 597 479 393 268 105
105 63 54 27
EcoO109I rG'GnC_Cy
Cuts at: 2950 2951 2950
Size: 1 5904
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2187 5052 1678
Size: 509 2865 2531
Fragments arranged by size:
2865 2531 509
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2583 2615 4546 4578 4620
Size: 32 135 32 1011 32 1931 32 42
Cuts at: 4620 4652 1373
Size: 32 2626
Fragments arranged by size:
2626 1931 1011 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 3249 3495 669
Size: 988 281 349 962 246 3079
Fragments arranged by size:
3079 988 962 349 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KasI G'GCGC_C
Cuts at: 2183 2183
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5019 5203 1305
Size: 3714 184 2007
Fragments arranged by size:
3714 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4083
Fragments arranged by size:
4083 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 2657 3863 4222 4381 518
Size: 648 1188 303 1206 359 159 2042
Fragments arranged by size:
2042 1206 1188 648 359 303 159
MspA1I CmG'CkG
Cuts at: 1606 2233 2275 3321 3998 5146 5391 1606
Size: 627 42 1046 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 677 627 245 42
NarI GG'CG_CC
Cuts at: 2184 2184
Size: 5905
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4414
Fragments arranged by size:
4414 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 3263 4808 1132
Size: 97 2034 1545 2229
Fragments arranged by size:
2229 2034 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PfoI T'CCnGG_A
Cuts at: 2111 2111
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2438 2470 3974 4006 5487 5519 2438
Size: 32 1504 32 1481 32 2824
Fragments arranged by size:
2824 1504 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2950 2950
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2176 2385 2406 2634 3257 830
Size: 1346 209 21 228 623 3478
Fragments arranged by size:
3478 1346 623 228 209 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 2275 2275
Size: 5905
SacI G_AGCT'C
Cuts at: 2111 2111
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2172 2381 2402 2553 2630 2853 3253
Size: 219 1127 209 21 151 77 223 400
Cuts at: 3253 4388 5069 5260 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1127 681 400 223 219 209
191 151 77 21
SfoI GGC'GCC
Cuts at: 2185 2185
Size: 5905
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 2643 1517
Size: 1126 4779
StyI C'CwwG_G
Cuts at: 513 1065 2004 3344 513
Size: 552 939 1340 3074
Fragments arranged by size:
3074 1340 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3932 3949 4102 4287 4732 325
Size: 1255 2352 17 153 185 445 1498
Fragments arranged by size:
2352 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4151 5863 96
Size: 274 80 33 51 1443 2174 1712 138
Fragments arranged by size:
2174 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2722 2722
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 2207 4034 2207
Size: 1827 4078
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI