Restriction map for
pFN21AE4379
(Circular) (Six-base) MAPSORT of: pFN21AE4379.seq Check: 8104 from: 1 to: 5902
pFN21AE4379 5902 bp
With 182 enzymes: SgfI *
March 28, 2012 14:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3245 1937
Size: 1308 4594
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2172 3141 3965 5349 5617 1632
Size: 540 969 824 1384 268 1917
Fragments arranged by size:
1917 1384 969 824 540 268
AflII C'TTAA_G
Cuts at: 820 1017 2354 820
Size: 197 1337 4368
Fragments arranged by size:
4368 1337 197
AflIII A'CryG_T
Cuts at: 1203 4801 5778 1203
Size: 3598 977 1327
Fragments arranged by size:
3598 1327 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 3020 3030 3645 5217 3020
Size: 10 615 1572 3705
Fragments arranged by size:
3705 1572 615 10
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 2585 2852 3211 3437 3589 5698 1493
Size: 1092 267 359 226 152 2109 1697
Fragments arranged by size:
2109 1697 1092 359 267 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2880 3227 1870
Size: 73 937 347 4545
Fragments arranged by size:
4545 937 347 73
BamHI G'GATC_C
Cuts at: 2805 3232 3599 2805
Size: 427 367 5108
Fragments arranged by size:
5108 427 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3223 4678 618
Size: 325 217 627 1436 1455 1842
Fragments arranged by size:
1842 1455 1436 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2676 3221 1391
Size: 1285 545 4072
Fragments arranged by size:
4072 1285 545
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 2439 2496 2969 928
Size: 971 279 261 57 473 3861
Fragments arranged by size:
3861 971 473 279 261 57
BbvCI CC'TCA_GC
Cuts at: 3069 3069
Size: 5902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 3963 5119 1790
Size: 2173 1156 2573
Fragments arranged by size:
2573 2173 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2330 2432 2965
Size: 416 53 83 399 24 913 102 533
Cuts at: 2965 3633 4591 442
Size: 668 958 1753
Fragments arranged by size:
1753 958 913 668 533 416 399 102
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2879 3098 4562 1239
Size: 1640 219 1464 2579
Fragments arranged by size:
2579 1640 1464 219
Bpu10I CC'TnA_GC
Cuts at: 3069 3069
Size: 5902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 4027 4892 5190 2255
Size: 1772 865 298 2967
Fragments arranged by size:
2967 1772 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3693 493
Size: 711 2489 2702
Fragments arranged by size:
2702 2489 711
BsaBI GATnn'nnATC
Cuts at: 3054 5896 3054
Size: 2842 3060
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3709 4091 4726 275
Size: 53 83 186 936 2176 382 635 1451
Fragments arranged by size:
2176 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4335 4733 5007 5154 5565 4335
Size: 398 274 147 411 4672
Fragments arranged by size:
4672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5677
Fragments arranged by size:
5677 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2723 3086 1186
Size: 1537 363 4002
Fragments arranged by size:
4002 1537 363
BseYI C'CCAG_C
Cuts at: 5105 5601 5105
Size: 496 5406
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3963 4048 5119 3963
Size: 85 1071 4746
Fragments arranged by size:
4746 1071 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2676 3221 3963 4048 5119 1391
Size: 399 886 545 742 85 1071 2174
Fragments arranged by size:
2174 1071 886 742 545 399 85
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2125 3257 844
Size: 1281 1132 3489
Fragments arranged by size:
3489 1281 1132
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2676 4397 4571 65
Size: 1183 1428 1721 174 1396
Fragments arranged by size:
1721 1428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2179 4546 4733 1700
Size: 110 369 2367 187 2869
Fragments arranged by size:
2869 2367 369 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 2478 4706 2478
Size: 2228 3674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstXI CCAn_nnnn'nTGG
Cuts at: 2320 2320
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2422 2805 2862 3232 3599 3984 4001 5453
Size: 450 383 57 370 367 385 17 1452
Cuts at: 5453 5539 5551 5897 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 450 385 383 370 367 346
86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 3418 4212 4232 3418
Size: 794 20 5088
Fragments arranged by size:
5088 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 2142 3206 2142
Size: 1064 4838
DraIII CAC_nnn'GTG
Cuts at: 2815 2815
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3349 4238 5867
Size: 54 1425 50 275 18 1519 889 1629
Cuts at: 5867 8
Size: 43
Fragments arranged by size:
1629 1519 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2058 2700 2825 3159 3832 1353
Size: 650 55 642 125 334 673 3423
Fragments arranged by size:
3423 673 650 642 334 125 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2879 3098 3141 3965 4562 5349
Size: 393 540 707 219 43 824 597 787
Cuts at: 5349 5617 1239
Size: 268 1524
Fragments arranged by size:
1524 824 787 707 597 540 393 268
219 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2053 2870 2053
Size: 817 5085
EcoO109I rG'GnC_Cy
Cuts at: 2251 2251
Size: 5902
EcoRI G'AATT_C
Cuts at: 2852 3211 3589 2852
Size: 359 378 5165
Fragments arranged by size:
5165 378 359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2087 2324 2087
Size: 237 5665
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 5049 1678
Size: 3371 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2168 2200 4543 4575 4617
Size: 32 135 32 596 32 2343 32 42
Cuts at: 4617 4649 1373
Size: 32 2626
Fragments arranged by size:
2626 2343 596 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2647 3246 3492 669
Size: 988 281 709 599 246 3079
Fragments arranged by size:
3079 988 709 599 281 246
HindIII A'AGCT_T
Cuts at: 748 2306 748
Size: 1558 4344
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2239 3086 5016 5200 1305
Size: 934 847 1930 184 2007
Fragments arranged by size:
2007 1930 934 847 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4080
Fragments arranged by size:
4080 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 4219 4378 518
Size: 648 2694 359 159 2042
Fragments arranged by size:
2694 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2396 2561 3318 3995 5143 5388 1606
Size: 790 165 757 677 1148 245 2120
Fragments arranged by size:
2120 1148 790 757 677 245 165
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5350
NdeI CA'TA_TG
Cuts at: 387 2086 387
Size: 1699 4203
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 3260 4805 1132
Size: 97 2031 1545 2229
Fragments arranged by size:
2229 2031 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2815 2996 1108
Size: 1707 181 4014
Fragments arranged by size:
4014 1707 181
PfoI T'CCnGG_A
Cuts at: 2856 2856
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 2251
Size: 5902
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PstI C_TGCA'G
Cuts at: 830 3254 830
Size: 2424 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
PvuII CAG'CTG
Cuts at: 2396 2561 2396
Size: 165 5737
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2268 3250 4385 5066 5257 826
Size: 219 1223 982 1135 681 191 1471
Fragments arranged by size:
1471 1223 1135 982 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 2882 3229 1872
Size: 1010 347 4545
Fragments arranged by size:
4545 1010 347
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 2354 4006 4907 5169 820
Size: 197 926 291 120 1652 901 262 1553
Fragments arranged by size:
1652 1553 926 901 291 262 197 120
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5902
StyI C'CwwG_G
Cuts at: 513 1065 3341 513
Size: 552 2276 3074
Fragments arranged by size:
3074 2276 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2115 3929 3946 4099 4284 4729 325
Size: 1255 535 1814 17 153 185 445 1498
Fragments arranged by size:
1814 1498 1255 535 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4148 5860 96
Size: 274 80 33 51 1443 2171 1712 138
Fragments arranged by size:
2171 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 3026 1316
Size: 1710 4192
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 2880 3227 1870
Size: 1010 347 4545
Fragments arranged by size:
4545 1010 347
XmnI GAAnn'nnTTC
Cuts at: 2126 2966 4031 2126
Size: 840 1065 3997
Fragments arranged by size:
3997 1065 840
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI