Restriction map for
pFN21AE4377
(Circular) (Six-base) MAPSORT of: pFN21AE4377.seq Check: 383 from: 1 to: 5902
pFN21AE4377 5902 bp
With 182 enzymes: SgfI *
March 28, 2012 14:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3063 3712 278
Size: 53 83 186 936 1527 649 2468
Fragments arranged by size:
2468 1527 936 649 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3054 3245 1937
Size: 1117 191 4594
Fragments arranged by size:
4594 1117 191
Acc65I G'GTAC_C
Cuts at: 2735 3223 2735
Size: 488 5414
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2783 3965 5349 5617 1632
Size: 1151 1182 1384 268 1917
Fragments arranged by size:
1917 1384 1182 1151 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 2346 2880 4801 5778 1203
Size: 1143 534 1921 977 1327
Fragments arranged by size:
1921 1327 1143 977 534
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 3645 5217 3645
Size: 1572 4330
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 2199 2417 2699 2815 2906 3211 3437 3589
Size: 706 218 282 116 91 305 226 152
Cuts at: 3589 5698 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 706 305 282 226 218 152
116 91
AseI AT'TA_AT
Cuts at: 160 1032 3180 4456 160
Size: 872 2148 1276 1606
Fragments arranged by size:
2148 1606 1276 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 3227 1870
Size: 73 1284 4545
Fragments arranged by size:
4545 1284 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2727 2760 2727
Size: 33 5869
BamHI G'GATC_C
Cuts at: 2092 3232 3599 2092
Size: 1140 367 4395
Fragments arranged by size:
4395 1140 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2531 2735 3223 4678 618
Size: 325 217 627 744 204 488 1455 1842
Fragments arranged by size:
1842 1455 744 627 488 325 217 204
BanII G_rGCy'C
Cuts at: 1391 2772 3221 1391
Size: 1381 449 4072
Fragments arranged by size:
4072 1381 449
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2242 928
Size: 971 343 4588
Fragments arranged by size:
4588 971 343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3026 3060 4093 4127 1903
Size: 34 1089 34 1033 34 3678
Fragments arranged by size:
3678 1089 1033 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 3963 5119 1790
Size: 2173 1156 2573
Fragments arranged by size:
2573 2173 1156
BmgBI CAC'GTC
Cuts at: 1318 2347 1318
Size: 1029 4873
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2143 2296 2941
Size: 416 53 83 399 24 726 153 645
Cuts at: 2941 3633 4591 442
Size: 692 958 1753
Fragments arranged by size:
1753 958 726 692 645 416 399 153
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2994 2994
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2091 2911 3043 4562 1239
Size: 852 820 132 1519 2579
Fragments arranged by size:
2579 1519 852 820 132
Bpu10I CC'TnA_GC
Cuts at: 2568 2568
Size: 5902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2511 4027 4892 5190 2511
Size: 1516 865 298 3223
Fragments arranged by size:
3223 1516 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2385 4565 882
Size: 617 886 2180 2219
Fragments arranged by size:
2219 2180 886 617
BsaAI yAC'GTr
Cuts at: 493 1204 3693 493
Size: 711 2489 2702
Fragments arranged by size:
2702 2489 711
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3060 3709 4091 4726
Size: 53 83 186 936 1527 649 382 635
Cuts at: 4726 275
Size: 1451
Fragments arranged by size:
1527 1451 936 649 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3112 4335 4733 5007 5154 5565 3112
Size: 1223 398 274 147 411 3449
Fragments arranged by size:
3449 1223 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2839 2869 3016 3046 1178
Size: 30 165 30 1436 30 147 30 4034
Fragments arranged by size:
4034 1436 165 147 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5902
BseYI C'CCAG_C
Cuts at: 2731 5105 5601 2731
Size: 2374 496 3032
Fragments arranged by size:
3032 2374 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2772 3963 4048 5119 2772
Size: 1191 85 1071 3555
Fragments arranged by size:
3555 1191 1071 85
BsmI GAATG_Cn'
Cuts at: 2056 3108 3413 3506 2056
Size: 1052 305 93 4452
Fragments arranged by size:
4452 1052 305 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2772 3221 3963 4048 5119 1391
Size: 399 982 449 742 85 1071 2174
Fragments arranged by size:
2174 1071 982 742 449 399 85
BspEI T'CCGG_A
Cuts at: 3112 5565 3112
Size: 2453 3449
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3257 844
Size: 2413 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3027 3789 1394
Size: 429 1204 762 3507
Fragments arranged by size:
3507 1204 762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4546 4733 1700
Size: 110 2736 187 2869
Fragments arranged by size:
2869 2736 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2092 2562 3232 3599 3984 4001 5453 5539
Size: 120 470 670 367 385 17 1452 86
Cuts at: 5539 5551 5897 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 670 470 385 367 346 120
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 2947 3418 4212 4232 2947
Size: 471 794 20 4617
Fragments arranged by size:
4617 794 471 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 2820 3206 2820
Size: 386 5516
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2494 2643 3349
Size: 54 1425 50 275 18 664 149 706
Cuts at: 3349 4238 5867 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 706 664 275 149 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3832 1353
Size: 2479 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2770 2770
Size: 5902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2783 2911 3043 3965 4562 5349
Size: 393 459 692 128 132 922 597 787
Cuts at: 5349 5617 1239
Size: 268 1524
Fragments arranged by size:
1524 922 787 692 597 459 393 268
132 128
EcoO109I rG'GnC_Cy
Cuts at: 2234 2606 3142 2234
Size: 372 536 4994
Fragments arranged by size:
4994 536 372
EcoRI G'AATT_C
Cuts at: 2199 2417 3211 3589 2199
Size: 218 794 378 4512
Fragments arranged by size:
4512 794 378 218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2720 2720
Size: 5902
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 5049 1678
Size: 3371 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3016 3048 3181 3213 4543
Size: 32 135 32 1444 32 133 32 1330
Cuts at: 4543 4575 4617 4649 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1444 1330 135 133 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3246 3492 669
Size: 988 281 1308 246 3079
Fragments arranged by size:
3079 1308 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 2739 3227 2739
Size: 488 5414
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5016 5200 1305
Size: 3711 184 2007
Fragments arranged by size:
3711 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2496 2645 10
Size: 54 1425 343 664 149 3267
Fragments arranged by size:
3267 1425 664 343 149 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2111 3005 3860 4219 4378 518
Size: 648 843 102 894 855 359 159 2042
Fragments arranged by size:
2042 894 855 843 648 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 3318 3995 5143 5388 1606
Size: 1712 677 1148 245 2120
Fragments arranged by size:
2120 1712 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5350
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 2884 3004 3260 4805 1132
Size: 97 1655 120 256 1545 2229
Fragments arranged by size:
2229 1655 1545 256 120 97
PasI CC'CwG_GG
Cuts at: 1348 2602 1348
Size: 1254 4648
PciI A'CATG_T
Cuts at: 2880 4801 2880
Size: 1921 3981
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2234 2606 2234
Size: 372 5530
PshAI GACnn'nnGTC
Cuts at: 1613 2232 3017 1613
Size: 619 785 4498
Fragments arranged by size:
4498 785 619
PsiI TTA'TAA
Cuts at: 3168 3472 3168
Size: 304 5598
PstI C_TGCA'G
Cuts at: 830 3019 3254 830
Size: 2189 235 3478
Fragments arranged by size:
3478 2189 235
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
SacI G_AGCT'C
Cuts at: 2772 2772
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SexAI A'CCwGG_T
Cuts at: 2375 2375
Size: 5902
SfcI C'TryA_G
Cuts at: 826 1045 2538 3015 3250 4385 5066 5257 826
Size: 219 1493 477 235 1135 681 191 1471
Fragments arranged by size:
1493 1471 1135 681 477 235 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2526 4006 4907 5169 820
Size: 197 926 583 1480 901 262 1553
Fragments arranged by size:
1553 1480 926 901 583 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5902
StyI C'CwwG_G
Cuts at: 513 1065 3155 3341 513
Size: 552 2090 186 3074
Fragments arranged by size:
3074 2090 552 186
SwaI ATTT'AAAT
Cuts at: 2820 2820
Size: 5902
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3929 3946 4099 4284 4729 325
Size: 1255 2349 17 153 185 445 1498
Fragments arranged by size:
2349 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2630 4148 5860
Size: 274 80 33 51 1443 653 1518 1712
Cuts at: 5860 96
Size: 138
Fragments arranged by size:
1712 1518 1443 653 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2856 2888 5862 5894 2856
Size: 32 2974 32 2864
Fragments arranged by size:
2974 2864 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 2828 3238 2828
Size: 410 5492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2311
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 2703 4031 2703
Size: 1328 4574
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3061 3710 276
Size: 53 83 186 936 1527 649 2468
Fragments arranged by size:
2468 1527 936 649 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SfiI SfoI SrfI