Restriction map for
pFN21AE4376
(Circular) (Six-base) MAPSORT of: pFN21AE4376.seq Check: 9103 from: 1 to: 5902
pFN21AE4376 5902 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3245 1937
Size: 1308 4594
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 2450 2519 2588 2657 2726 3175 3965
Size: 749 69 69 69 69 69 449 790
Cuts at: 3965 5349 5617 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 790 749 449 268 69 69
69 69 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 4801 5778 1203
Size: 3598 977 1327
Fragments arranged by size:
3598 1327 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 2038 2281 2350 2419 2488 2557 2626 2695 3129
Size: 243 69 69 69 69 69 69 434
Cuts at: 3129 3645 5217 2038
Size: 516 1572 2723
Fragments arranged by size:
2723 1572 516 434 243 69 69 69
69 69 69
ApaI G_GGCC'C
Cuts at: 2908 2908
Size: 5902
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 3113 3211 3437 3589 5698 1493
Size: 1620 98 226 152 2109 1697
Fragments arranged by size:
2109 1697 1620 226 152 98
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 3227 1870
Size: 73 1284 4545
Fragments arranged by size:
4545 1284 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 3223 4678 618
Size: 325 217 627 363 1073 1455 1842
Fragments arranged by size:
1842 1455 1073 627 363 325 217
BanII G_rGCy'C
Cuts at: 1391 2129 2313 2908 3221 1391
Size: 738 184 595 313 4072
Fragments arranged by size:
4072 738 595 313 184
BbeI G_GCGC'C
Cuts at: 2154 2154
Size: 5902
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2783 3108 928
Size: 971 884 325 3722
Fragments arranged by size:
3722 971 884 325
BbvCI CC'TCA_GC
Cuts at: 2931 2931
Size: 5902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2337 2406 2475 2544 2613 2682 5897 2337
Size: 69 69 69 69 69 3215 2342
Fragments arranged by size:
3215 2342 69 69 69 69 69
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 2908 3963 5119 1790
Size: 1118 1055 1156 2573
Fragments arranged by size:
2573 1156 1118 1055
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3633 4591 442
Size: 416 53 83 399 24 2216 958 1753
Fragments arranged by size:
2216 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4562 1239
Size: 3323 2579
Bpu10I CC'TnA_GC
Cuts at: 2931 2931
Size: 5902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4027 4892 5190 4027
Size: 865 298 4739
Fragments arranged by size:
4739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3018 3693 493
Size: 711 1814 675 2702
Fragments arranged by size:
2702 1814 711 675
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 3709 4091 4726
Size: 53 83 186 936 618 1558 382 635
Cuts at: 4726 275
Size: 1451
Fragments arranged by size:
1558 1451 936 635 618 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4335 4733 5007 5154 5565 4335
Size: 398 274 147 411 4672
Fragments arranged by size:
4672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2963 2993 1178
Size: 30 165 30 1560 30 4087
Fragments arranged by size:
4087 1560 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2948 1186
Size: 973 789 4140
Fragments arranged by size:
4140 973 789
BseYI C'CCAG_C
Cuts at: 2038 2189 2231 2286 2355 2700 2859 2907 3050
Size: 151 42 55 69 345 159 48 143
Cuts at: 3050 5105 5601 2038
Size: 2055 496 2339
Fragments arranged by size:
2339 2055 496 345 159 151 143 69
55 48 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 1163
Size: 573 315 5014
Fragments arranged by size:
5014 573 315
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2129 2313 3963 4048 5119 2129
Size: 184 1650 85 1071 2912
Fragments arranged by size:
2912 1650 1071 184 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
BsmBI CGTCTCn'nnnn_
Cuts at: 3189 3189
Size: 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2313 2908 3221 3963 4048 5119
Size: 399 339 184 595 313 742 85 1071
Cuts at: 5119 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 595 399 339 313 184
85
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3257 844
Size: 2413 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3029 4397 4571 65
Size: 1183 1781 1368 174 1396
Fragments arranged by size:
1781 1396 1368 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4546 4733 1700
Size: 110 2736 187 2869
Fragments arranged by size:
2869 2736 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2056 2337 2406 2475 2544 2613 2682 3165
Size: 84 281 69 69 69 69 69 483
Cuts at: 3165 3232 3599 3984 4001 5453 5539 5551 5897
Size: 67 367 385 17 1452 86 12 346
Cuts at: 5897 1972
Size: 1977
Fragments arranged by size:
1977 1452 483 385 367 346 281 86
84 69 69 69 69 69 67 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2146 513
Size: 552 1081 4269
Fragments arranged by size:
4269 1081 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 2307 2376 2445 2514 2583 2652 2721 3130 3418
Size: 69 69 69 69 69 69 409 288
Cuts at: 3418 4212 4232 2307
Size: 794 20 3977
Fragments arranged by size:
3977 794 409 288 69 69 69 69
69 69 20
ClaI AT'CG_AT
Cuts at: 1554 3142 1554
Size: 1588 4314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2143 2202
Size: 54 1425 50 275 18 286 27 59
Cuts at: 2202 3349 4238 5867 8
Size: 1147 889 1629 43
Fragments arranged by size:
1629 1425 1147 889 286 275 59 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3832 1353
Size: 2479 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2127 2311 2127
Size: 184 5718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2450 2519 2588 2657 2726 3175
Size: 393 749 69 69 69 69 69 449
Cuts at: 3175 3965 4562 5349 5617 1239
Size: 790 597 787 268 1524
Fragments arranged by size:
1524 790 787 749 597 449 393 268
69 69 69 69 69
EcoNI CCTnn'n_nnAGG
Cuts at: 2243 3005 2243
Size: 762 5140
EcoO109I rG'GnC_Cy
Cuts at: 2194 2227 2904 2194
Size: 33 677 5192
Fragments arranged by size:
5192 677 33
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 2154 5049 1678
Size: 476 2895 2531
Fragments arranged by size:
2895 2531 476
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4543 4575 4617 4649 1373
Size: 32 135 32 2971 32 42 32 2626
Fragments arranged by size:
2971 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3154 3246 3492 669
Size: 988 281 1216 92 246 3079
Fragments arranged by size:
3079 1216 988 281 246 92
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KasI G'GCGC_C
Cuts at: 2150 2150
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2084 5016 5200 1305
Size: 779 2932 184 2007
Fragments arranged by size:
2932 2007 779 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2118 2145 2204 10
Size: 54 1425 343 286 27 59 3708
Fragments arranged by size:
3708 1425 343 286 59 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 4219 4378 518
Size: 648 2694 359 159 2042
Fragments arranged by size:
2694 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2359 2704 2863 2953 3061 3318 3995
Size: 432 321 345 159 90 108 257 677
Cuts at: 3995 5143 5388 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 432 345 321 257 245
159 108 90
NarI GG'CG_CC
Cuts at: 2151 2151
Size: 5902
NcoI C'CATG_G
Cuts at: 513 1065 2146 513
Size: 552 1081 4269
Fragments arranged by size:
4269 1081 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 3260 4805 1132
Size: 97 2031 1545 2229
Fragments arranged by size:
2229 2031 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2817 1108
Size: 1709 4193
PfoI T'CCnGG_A
Cuts at: 2046 2963 2981 2046
Size: 917 18 4967
Fragments arranged by size:
4967 917 18
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PmlI CAC'GTG
Cuts at: 3018 3018
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 2227 2194
Size: 33 5869
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PspOMI G'GGCC_C
Cuts at: 2904 2904
Size: 5902
PstI C_TGCA'G
Cuts at: 830 2302 2334 2371 2403 2440 2472 2509 2541
Size: 1472 32 37 32 37 32 37 32
Cuts at: 2541 2578 2610 2647 2679 2716 2958 3254 830
Size: 37 32 37 32 37 242 296 3478
Fragments arranged by size:
3478 1472 296 242 37 37 37 37
37 37 32 32 32 32 32 32
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
PvuII CAG'CTG
Cuts at: 2038 2359 2704 2953 2038
Size: 321 345 249 4987
Fragments arranged by size:
4987 345 321 249
SacI G_AGCT'C
Cuts at: 2129 2313 2129
Size: 184 5718
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2130 2298 2330 2367 2399 2436 2468
Size: 219 1085 168 32 37 32 37 32
Cuts at: 2468 2505 2537 2574 2606 2643 2675 2712 2948
Size: 37 32 37 32 37 32 37 236
Cuts at: 2948 2954 3250 4385 5066 5257 826
Size: 6 296 1135 681 191 1471
Fragments arranged by size:
1471 1135 1085 681 296 236 219 191
168 37 37 37 37 37 37 32
32 32 32 32 32 6
SfoI GGC'GCC
Cuts at: 2152 2152
Size: 5902
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4006 4907 5169 820
Size: 197 926 2063 901 262 1553
Fragments arranged by size:
2063 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5902
StyI C'CwwG_G
Cuts at: 513 1065 2016 2024 2146 2223 2811 2830 3341
Size: 552 951 8 122 77 588 19 511
Cuts at: 3341 513
Size: 3074
Fragments arranged by size:
3074 951 588 552 511 122 77 19
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2294 2363 2432 2501 2570 2639 2708
Size: 1255 714 69 69 69 69 69 69
Cuts at: 2708 2783 3016 3064 3929 3946 4099 4284 4729
Size: 75 233 48 865 17 153 185 445
Cuts at: 4729 325
Size: 1498
Fragments arranged by size:
1498 1255 865 714 445 233 185 153
75 69 69 69 69 69 69 48
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4148 5860 96
Size: 274 80 33 51 1443 2171 1712 138
Fragments arranged by size:
2171 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3002 3034 3039 3071 5862 5894 3002
Size: 32 5 32 2791 32 3010
Fragments arranged by size:
3010 2791 32 32 32 5
Tth111I GACn'n_nGTC
Cuts at: 1316 2879 1316
Size: 1563 4339
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 2111
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 4031 4031
Size: 5902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI