Restriction map for
pFN21AE4374
(Circular) (Six-base) MAPSORT of: pFN21AE4374.seq Check: 1633 from: 1 to: 5881
pFN21AE4374 5881 bp
With 182 enzymes: SgfI *
March 28, 2012 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2335 2335
Size: 5881
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2820 2905 3224 1937
Size: 883 85 319 4594
Fragments arranged by size:
4594 883 319 85
Acc65I G'GTAC_C
Cuts at: 3202 3202
Size: 5881
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3152 3944 5328 5596 1632
Size: 1520 792 1384 268 1917
Fragments arranged by size:
1917 1520 1384 792 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5684
AflIII A'CryG_T
Cuts at: 1203 4780 5757 1203
Size: 3577 977 1327
Fragments arranged by size:
3577 1327 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 2795 1166
Size: 1629 4252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 2974 3624 5196 2974
Size: 650 1572 3659
Fragments arranged by size:
3659 1572 650
ApaI G_GGCC'C
Cuts at: 2712 2778 2983 2712
Size: 66 205 5610
Fragments arranged by size:
5610 205 66
ApaLI G'TGCA_C
Cuts at: 3044 3938 5094 3044
Size: 894 1156 3831
Fragments arranged by size:
3831 1156 894
ApoI r'AATT_y
Cuts at: 1493 3190 3416 3568 5677 1493
Size: 1697 226 152 2109 1697
Fragments arranged by size:
2109 1697 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 3016 3206 1870
Size: 73 1073 190 4545
Fragments arranged by size:
4545 1073 190 73
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2495 2597 3020 3202 4657
Size: 325 217 627 708 102 423 182 1455
Cuts at: 4657 618
Size: 1842
Fragments arranged by size:
1842 1455 708 627 423 325 217 182
102
BanII G_rGCy'C
Cuts at: 1391 2166 2538 2712 2778 2983 3160 3200 1391
Size: 775 372 174 66 205 177 40 4072
Fragments arranged by size:
4072 775 372 205 177 174 66 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2186 928
Size: 971 287 4623
Fragments arranged by size:
4623 971 287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4072 4106 1903
Size: 34 2135 34 3678
Fragments arranged by size:
3678 2135 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4492 136
Size: 107 122 71 631 605 2820 1525
Fragments arranged by size:
2820 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2028 5876 2028
Size: 3848 2033
BlpI GC'TnA_GC
Cuts at: 2392 3298 2392
Size: 906 4975
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2712 2778 2983 3023 3048 3942 5098
Size: 248 674 66 205 40 25 894 1156
Cuts at: 5098 1790
Size: 2573
Fragments arranged by size:
2573 1156 894 674 248 205 66 40
25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2485 2585 3035
Size: 416 53 83 399 24 1068 100 450
Cuts at: 3035 3612 4570 442
Size: 577 958 1753
Fragments arranged by size:
1753 1068 958 577 450 416 399 100
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2077 2266 2450 2459 2631 2867 2907 3005
Size: 838 189 184 9 172 236 40 98
Cuts at: 3005 4541 1239
Size: 1536 2579
Fragments arranged by size:
2579 1536 838 236 189 184 172 98
40 9
Bpu10I CC'TnA_GC
Cuts at: 3102 3102
Size: 5881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2723 4006 4871 5169 2723
Size: 1283 865 298 3435
Fragments arranged by size:
3435 1283 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2635 4544 882
Size: 617 1136 1909 2219
Fragments arranged by size:
2219 1909 1136 617
BsaAI yAC'GTr
Cuts at: 493 1204 3672 493
Size: 711 2468 2702
Fragments arranged by size:
2702 2468 711
BsaBI GATnn'nnATC
Cuts at: 5875 5875
Size: 5881
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2858 3688 4070 4705
Size: 53 83 186 936 1325 830 382 635
Cuts at: 4705 275
Size: 1451
Fragments arranged by size:
1451 1325 936 830 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2379 4314 4712 4986 5133 5544 2379
Size: 1935 398 274 147 411 2716
Fragments arranged by size:
2716 1935 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2261 2291 2604 2634 3128
Size: 30 165 30 858 30 313 30 494
Cuts at: 3128 3158 1178
Size: 30 3901
Fragments arranged by size:
3901 858 494 313 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 2269 3002 1186
Size: 894 189 733 4065
Fragments arranged by size:
4065 894 733 189
BseYI C'CCAG_C
Cuts at: 2226 2272 2542 2647 5084 5580 2226
Size: 46 270 105 2437 496 2527
Fragments arranged by size:
2527 2437 496 270 105 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 1163
Size: 573 516 4792
Fragments arranged by size:
4792 573 516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3331 4092 5120 1540
Size: 275 186 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2523 3048 3942 4027 5098 2523
Size: 525 894 85 1071 3306
Fragments arranged by size:
3306 1071 894 525 85
BsmI GAATG_Cn'
Cuts at: 3392 3485 3392
Size: 93 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2166 2523 2538 2712 2778 2983
Size: 399 248 128 357 15 174 66 205
Cuts at: 2983 3023 3048 3160 3200 3942 4027 5098 1391
Size: 40 25 112 40 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 357 248 205 174
128 112 85 66 40 40 25 15
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 3770 5500 3770
Size: 1730 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 2335 3236 844
Size: 1277 214 901 3489
Fragments arranged by size:
3489 1277 901 214
BsrBI CCG'CTC
Cuts at: 1394 1823 2018 3768 1394
Size: 429 195 1750 3507
Fragments arranged by size:
3507 1750 429 195
BsrDI GCAATG_nn'
Cuts at: 65 1248 4376 4550 65
Size: 1183 3128 174 1396
Fragments arranged by size:
3128 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2451 2492 4525 4712 1700
Size: 110 641 41 2033 187 2869
Fragments arranged by size:
2869 2033 641 187 110 41
BsrGI T'GTAC_A
Cuts at: 96 3057 96
Size: 2961 2920
BssHII G'CGCG_C
Cuts at: 1925 2563 2565 1925
Size: 638 2 5241
Fragments arranged by size:
5241 638 2
BssSI C'ACGA_G
Cuts at: 3161 3941 4953 3161
Size: 780 1012 4089
Fragments arranged by size:
4089 1012 780
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2974 3027 794
Size: 2180 53 3648
Fragments arranged by size:
3648 2180 53
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstXI CCAn_nnnn'nTGG
Cuts at: 2837 2837
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 2028 3138 3211 3578 3963 3980 5432 5518
Size: 56 1110 73 367 385 17 1452 86
Cuts at: 5518 5530 5876 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1110 385 367 346 86 73
56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3087 513
Size: 552 939 1083 3307
Fragments arranged by size:
3307 1083 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 3397 4191 4211 3397
Size: 794 20 5067
Fragments arranged by size:
5067 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2677 2712 529
Size: 35 2113 35 3698
Fragments arranged by size:
3698 2113 35 35
DraI TTT'AAA
Cuts at: 3185 3185
Size: 5881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2640 3328
Size: 54 1425 50 275 18 177 633 688
Cuts at: 3328 4217 5846 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 688 633 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3328 1537
Size: 275 1516 4090
Fragments arranged by size:
4090 1516 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2360 3811 1353
Size: 1007 1451 3423
Fragments arranged by size:
3423 1451 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2348 2438 4468 4842 4988 387
Size: 1064 138 759 90 2030 374 146 1280
Fragments arranged by size:
2030 1280 1064 759 374 146 138 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2077 2266 2450 2459 2631 2867 2907
Size: 393 445 189 184 9 172 236 40
Cuts at: 2907 3005 3152 3944 4541 5328 5596 1239
Size: 98 147 792 597 787 268 1524
Fragments arranged by size:
1524 792 787 597 445 393 268 236
189 184 172 147 98 40 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2987 2987
Size: 5881
EcoO109I rG'GnC_Cy
Cuts at: 2479 2758 2980 2479
Size: 279 222 5380
Fragments arranged by size:
5380 279 222
EcoRI G'AATT_C
Cuts at: 3190 3568 3190
Size: 378 5503
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 5028 1678
Size: 3350 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4522 4554 4596 4628 1373
Size: 32 135 32 2950 32 42 32 2626
Fragments arranged by size:
2950 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2042 748
Size: 1294 4587
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 3206 3206
Size: 5881
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4995 5179 1305
Size: 3690 184 2007
Fragments arranged by size:
3690 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4059
Fragments arranged by size:
4059 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2462 2795 2835 3839 4198 4357
Size: 648 1035 261 333 40 1004 359 159
Cuts at: 4357 518
Size: 2042
Fragments arranged by size:
2042 1035 1004 648 359 333 261 159
40
MspA1I CmG'CkG
Cuts at: 1606 2119 2305 2866 3297 3974 5122 5367 1606
Size: 513 186 561 431 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 561 513 431 245 186
NaeI GCC'GGC
Cuts at: 2453 2494 2453
Size: 41 5840
NcoI C'CATG_G
Cuts at: 513 1065 2004 3087 513
Size: 552 939 1083 3307
Fragments arranged by size:
3307 1083 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NgoMIV G'CCGG_C
Cuts at: 2451 2492 2451
Size: 41 5840
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 2200 3239 4784 1132
Size: 97 971 1039 1545 2229
Fragments arranged by size:
2229 1545 1039 971 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5881
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5881
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2522 2554 2745 2777 3155 3187 3950 3982 5463
Size: 32 191 32 378 32 763 32 1481
Cuts at: 5463 5495 2522
Size: 32 2908
Fragments arranged by size:
2908 1481 763 378 191 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2479 2758 2479
Size: 279 5602
PshAI GACnn'nnGTC
Cuts at: 1613 2627 1613
Size: 1014 4867
PsiI TTA'TAA
Cuts at: 3451 3451
Size: 5881
PspOMI G'GGCC_C
Cuts at: 2708 2774 2979 2708
Size: 66 205 5610
Fragments arranged by size:
5610 205 66
PstI C_TGCA'G
Cuts at: 830 2110 2118 2304 3233 830
Size: 1280 8 186 929 3478
Fragments arranged by size:
3478 1280 929 186 8
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 2119 2305 2119
Size: 186 5695
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SanDI GG'GwC_CC
Cuts at: 2479 2479
Size: 5881
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 2106 2114 2300 3229 4364 5045 5236
Size: 219 1061 8 186 929 1135 681 191
Cuts at: 5236 826
Size: 1471
Fragments arranged by size:
1471 1135 1061 929 681 219 191 186
8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 3208 1872
Size: 1336 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2738 3985 4886 5148 820
Size: 197 926 795 1247 901 262 1553
Fragments arranged by size:
1553 1247 926 901 795 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 2004 2057 3087 3320 513
Size: 552 939 53 1030 233 3074
Fragments arranged by size:
3074 1030 939 552 233 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2286 2534 2585 2656 3908 3925 4078
Size: 1255 706 248 51 71 1252 17 153
Cuts at: 4078 4263 4708 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1252 706 445 248 185 153
71 51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3057 4127 5839
Size: 274 80 33 51 1443 1080 1070 1712
Cuts at: 5839 96
Size: 138
Fragments arranged by size:
1712 1443 1080 1070 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2261 2293 2604 2636 5841 5873 2261
Size: 32 311 32 3205 32 2269
Fragments arranged by size:
3205 2269 311 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2477 2901 1316
Size: 1161 424 4296
Fragments arranged by size:
4296 1161 424
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 3206 1870
Size: 1336 4545
XmnI GAAnn'nnTTC
Cuts at: 2324 4010 2324
Size: 1686 4195
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI