Restriction map for
pFN21AE4372
(Circular) (Six-base) MAPSORT of: pFN21AE4372.seq Check: 1408 from: 1 to: 5875
pFN21AE4372 5875 bp
With 182 enzymes: SgfI *
March 28, 2012 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2503 2503
Size: 5875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3218 1937
Size: 1281 4594
Acc65I G'GTAC_C
Cuts at: 3196 3196
Size: 5875
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3938 5322 5590 1632
Size: 2306 1384 268 1917
Fragments arranged by size:
2306 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 2466 4774 5751 1203
Size: 1263 2308 977 1327
Fragments arranged by size:
2308 1327 1263 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 2165 2283 1166
Size: 999 118 4758
Fragments arranged by size:
4758 999 118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2487 1184
Size: 1303 4572
AlwNI CAG_nnn'CTG
Cuts at: 3618 5190 3618
Size: 1572 4303
ApaI G_GGCC'C
Cuts at: 2841 2841
Size: 5875
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 2403 2922 3184 3410 3562 5671 1493
Size: 910 519 262 226 152 2109 1697
Fragments arranged by size:
2109 1697 910 519 262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 2382 3200 1870
Size: 73 439 818 4545
Fragments arranged by size:
4545 818 439 73
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2515 3140 3196 4651 618
Size: 325 217 627 728 625 56 1455 1842
Fragments arranged by size:
1842 1455 728 627 625 325 217 56
BanII G_rGCy'C
Cuts at: 1391 2190 2365 2841 3194 1391
Size: 799 175 476 353 4072
Fragments arranged by size:
4072 799 476 353 175
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4904
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2482 2516 2579 2613 4066 4100 1903
Size: 34 545 34 63 34 1453 34 3678
Fragments arranged by size:
3678 1453 545 63 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2360 4486 136
Size: 107 122 71 631 605 688 2126 1525
Fragments arranged by size:
2126 1525 688 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2825 5870 2825
Size: 3045 2830
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 2520 2841 3936 5092 1790
Size: 730 321 1095 1156 2573
Fragments arranged by size:
2573 1156 1095 730 321
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3049 3606 4564
Size: 416 53 83 399 24 1632 557 958
Cuts at: 4564 442
Size: 1753
Fragments arranged by size:
1753 1632 958 557 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2423 2423
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2916 3097 4535 1239
Size: 1097 580 181 1438 2579
Fragments arranged by size:
2579 1438 1097 580 181
Bpu10I CC'TnA_GC
Cuts at: 3075 3075
Size: 5875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2948 4000 4865 5163 2948
Size: 1052 865 298 3660
Fragments arranged by size:
3660 1052 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4538 882
Size: 617 3039 2219
Fragments arranged by size:
3039 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3666 493
Size: 711 2462 2702
Fragments arranged by size:
2702 2462 711
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2499 2529 3095 3125 1178
Size: 30 165 30 1096 30 566 30 3928
Fragments arranged by size:
3928 1096 566 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5875
BseYI C'CCAG_C
Cuts at: 2528 3094 5078 5574 2528
Size: 566 1984 496 2829
Fragments arranged by size:
2829 1984 566 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5302
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3069 3325 4086 5114 1540
Size: 275 186 1068 256 761 1028 2301
Fragments arranged by size:
2301 1068 1028 761 275 256 186
BsiHKAI G_wGCw'C
Cuts at: 2172 3936 4021 5092 2172
Size: 1764 85 1071 2955
Fragments arranged by size:
2955 1764 1071 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
BsmBI CGTCTCn'nnnn_
Cuts at: 2032 2032
Size: 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2190 2365 2520 2841 3194 3936
Size: 399 382 18 175 155 321 353 742
Cuts at: 3936 4021 5092 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 382 353 321 175
155 85 18
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 3764 5494 3764
Size: 1730 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2503 3230 844
Size: 1659 727 3489
Fragments arranged by size:
3489 1659 727
BsrBI CCG'CTC
Cuts at: 1394 1823 2711 3762 1394
Size: 429 888 1051 3507
Fragments arranged by size:
3507 1051 888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2500 4370 4544 65
Size: 1183 1252 1870 174 1396
Fragments arranged by size:
1870 1396 1252 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 2136 2321 4519 4706 1700
Size: 110 249 77 185 2198 187 2869
Fragments arranged by size:
2869 2198 249 187 185 110 77
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 2913 3935 4947 2913
Size: 1022 1012 3841
Fragments arranged by size:
3841 1022 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2102 794
Size: 1308 4567
BstBI TT'CG_AA
Cuts at: 2619 4679 2619
Size: 2060 3815
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5875
BstXI CCAn_nnnn'nTGG
Cuts at: 2848 2848
Size: 5875
BstYI r'GATC_y
Cuts at: 1972 2825 3205 3572 3957 3974 5426 5512 5524
Size: 853 380 367 385 17 1452 86 12
Cuts at: 5524 5870 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 853 385 380 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2872 3063 513
Size: 552 1807 191 3325
Fragments arranged by size:
3325 1807 552 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2604 487
Size: 683 56 1378 3758
Fragments arranged by size:
3758 1378 683 56
BtsI GCAGTG_nn'
Cuts at: 3391 4185 4205 3391
Size: 794 20 5061
Fragments arranged by size:
5061 794 20
ClaI AT'CG_AT
Cuts at: 1554 2459 1554
Size: 905 4970
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2897 4882 809
Size: 507 1581 1985 1802
Fragments arranged by size:
1985 1802 1581 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3066 3322 4211
Size: 54 1425 50 275 18 1236 256 889
Cuts at: 4211 5840 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1236 889 275 256 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3066 3322 1537
Size: 275 1254 256 4090
Fragments arranged by size:
4090 1254 275 256
EarI CTCTTCn'nnn_
Cuts at: 1353 3084 3805 1353
Size: 1731 721 3423
Fragments arranged by size:
3423 1731 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4462 4836 4982 387
Size: 1064 138 2873 374 146 1280
Fragments arranged by size:
2873 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2916 3097 3938 4535 5322 5590
Size: 393 704 580 181 841 597 787 268
Cuts at: 5590 1239
Size: 1524
Fragments arranged by size:
1524 841 787 704 597 580 393 268
181
EcoO109I rG'GnC_Cy
Cuts at: 2150 2386 2837 2150
Size: 236 451 5188
Fragments arranged by size:
5188 451 236
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
EcoRV GAT'ATC
Cuts at: 2779 2779
Size: 5875
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 2049 2529 5022 1678
Size: 371 480 2493 2531
Fragments arranged by size:
2531 2493 480 371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4516 4548 4590 4622 1373
Size: 32 135 32 2944 32 42 32 2626
Fragments arranged by size:
2944 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2298 2622 748
Size: 1550 324 4001
Fragments arranged by size:
4001 1550 324
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 3200 3200
Size: 5875
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4989 5173 1305
Size: 3684 184 2007
Fragments arranged by size:
3684 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4053
Fragments arranged by size:
4053 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2283 2469 2846 3833 4192 4351
Size: 648 999 118 186 377 987 359 159
Cuts at: 4351 518
Size: 2042
Fragments arranged by size:
2042 999 987 648 377 359 186 159
118
MspA1I CmG'CkG
Cuts at: 1606 2245 3291 3968 5116 5361 1606
Size: 639 1046 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 677 639 245
NaeI GCC'GGC
Cuts at: 2061 2323 2061
Size: 262 5613
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5323
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NgoMIV G'CCGG_C
Cuts at: 2059 2321 2059
Size: 262 5613
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 2470 3233 4778 1132
Size: 97 1241 763 1545 2229
Fragments arranged by size:
2229 1545 1241 763 97
PasI CC'CwG_GG
Cuts at: 1348 2521 1348
Size: 1173 4702
PciI A'CATG_T
Cuts at: 2466 4774 2466
Size: 2308 3567
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3150 1108
Size: 2042 3833
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PspOMI G'GGCC_C
Cuts at: 2837 2837
Size: 5875
PstI C_TGCA'G
Cuts at: 830 2859 3227 830
Size: 2029 368 3478
Fragments arranged by size:
3478 2029 368
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 2245 2245
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SapI GCTCTTCn'nnn_
Cuts at: 3084 3084
Size: 5875
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SfcI C'TryA_G
Cuts at: 826 1045 2197 2855 3223 4358 5039 5230 826
Size: 219 1152 658 368 1135 681 191 1471
Fragments arranged by size:
1471 1152 1135 681 658 368 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 2384 3202 1872
Size: 512 818 4545
Fragments arranged by size:
4545 818 512
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2963 3979 4880 5142 820
Size: 197 926 1020 1016 901 262 1553
Fragments arranged by size:
1553 1020 1016 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5875
StyI C'CwwG_G
Cuts at: 513 1065 3314 513
Size: 552 2249 3074
Fragments arranged by size:
3074 2249 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3902 3919 4072 4257 4702 325
Size: 1255 2322 17 153 185 445 1498
Fragments arranged by size:
2322 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4121 5833 96
Size: 274 80 33 51 1443 2144 1712 138
Fragments arranged by size:
2144 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2499 2531 2768 2800 3055 3087 3095 3127 5835
Size: 32 237 32 255 32 8 32 2708
Cuts at: 5835 5867 2499
Size: 32 2507
Fragments arranged by size:
2708 2507 255 237 32 32 32 32
32 8
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 2382 3200 1870
Size: 512 818 4545
Fragments arranged by size:
4545 818 512
XmnI GAAnn'nnTTC
Cuts at: 2922 4004 2922
Size: 1082 4793
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI