Restriction map for
pFN21AE4371
(Circular) (Six-base) MAPSORT of: pFN21AE4371.seq Check: 1626 from: 1 to: 5875
pFN21AE4371 5875 bp
With 182 enzymes: SgfI *
March 28, 2012 13:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2307 2307
Size: 5875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3218 1937
Size: 1281 4594
Acc65I G'GTAC_C
Cuts at: 3196 3196
Size: 5875
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2136 2582 3938 5322 5590 1632
Size: 504 446 1356 1384 268 1917
Fragments arranged by size:
1917 1384 1356 504 446 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2186 2552 1184
Size: 1002 366 4507
Fragments arranged by size:
4507 1002 366
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2495 2527 2495
Size: 32 5843
AlwNI CAG_nnn'CTG
Cuts at: 3104 3173 3618 5190 3104
Size: 69 445 1572 3789
Fragments arranged by size:
3789 1572 445 69
ApaI G_GGCC'C
Cuts at: 2018 2203 2409 2018
Size: 185 206 5484
Fragments arranged by size:
5484 206 185
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 3184 3410 3562 5671 1493
Size: 1691 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 2018 3200 1870
Size: 73 75 1182 4545
Fragments arranged by size:
4545 1182 75 73
BamHI G'GATC_C
Cuts at: 2854 3205 3572 2854
Size: 351 367 5157
Fragments arranged by size:
5157 367 351
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2116 2266 2869 3196 4651
Size: 325 217 627 329 150 603 327 1455
Cuts at: 4651 618
Size: 1842
Fragments arranged by size:
1842 1455 627 603 329 327 325 217
150
BanII G_rGCy'C
Cuts at: 1391 2018 2189 2203 2409 2627 2813 3194 1391
Size: 627 171 14 206 218 186 381 4072
Fragments arranged by size:
4072 627 381 218 206 186 171 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 928
Size: 971 325 4579
Fragments arranged by size:
4579 971 325
BbvCI CC'TCA_GC
Cuts at: 3166 3166
Size: 5875
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4066 4100 1903
Size: 34 50 34 2045 34 3678
Fragments arranged by size:
3678 2045 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 2364 4486
Size: 107 122 71 631 605 388 304 2122
Cuts at: 4486 136
Size: 1525
Fragments arranged by size:
2122 1525 631 605 388 304 122 107
71
BglII A'GATC_T
Cuts at: 5870 5870
Size: 5875
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2121 2203 2271 2409 2703 3069 3936
Size: 228 103 82 68 138 294 366 867
Cuts at: 3936 5092 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 867 366 294 228 138 103
82 68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3606 4564 442
Size: 416 53 83 399 24 2189 958 1753
Fragments arranged by size:
2189 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 3181 2110
Size: 1071 4804
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2618 2702 3023 4535 1239
Size: 1379 84 321 1512 2579
Fragments arranged by size:
2579 1512 1379 321 84
Bpu10I CC'TnA_GC
Cuts at: 2639 3166 2639
Size: 527 5348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4000 4865 5163 4000
Size: 865 298 4712
Fragments arranged by size:
4712 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2985 4538 882
Size: 617 1486 1553 2219
Fragments arranged by size:
2219 1553 1486 617
BsaAI yAC'GTr
Cuts at: 493 1204 3666 493
Size: 711 2462 2702
Fragments arranged by size:
2702 2462 711
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5650
Fragments arranged by size:
5650 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 2768 3094 1186
Size: 1105 477 326 3967
Fragments arranged by size:
3967 1105 477 326
BseYI C'CCAG_C
Cuts at: 2202 2370 3015 5078 5574 2202
Size: 168 645 2063 496 2503
Fragments arranged by size:
2503 2063 645 496 168
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2009 1163
Size: 573 273 5029
Fragments arranged by size:
5029 573 273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2368 3325 4086 5114 1540
Size: 275 186 367 957 761 1028 2301
Fragments arranged by size:
2301 1028 957 761 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 2813 3936 4021 5092 2813
Size: 1123 85 1071 3596
Fragments arranged by size:
3596 1123 1071 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2121 2189 2203 2271 2409 2627
Size: 399 228 103 68 14 68 138 218
Cuts at: 2627 2703 2813 3069 3194 3936 4021 5092 1391
Size: 76 110 256 125 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 256 228 218 138
125 110 103 85 76 68 68 14
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 3764 5494 3764
Size: 1730 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2307 2685 3230 844
Size: 1463 378 545 3489
Fragments arranged by size:
3489 1463 545 378
BsrBI CCG'CTC
Cuts at: 1394 1823 2589 2748 3762 1394
Size: 429 766 159 1014 3507
Fragments arranged by size:
3507 1014 766 429 159
BsrDI GCAATG_nn'
Cuts at: 65 1248 4370 4544 65
Size: 1183 3122 174 1396
Fragments arranged by size:
3122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2358 2478 2556 2823 4519 4706 1700
Size: 110 548 120 78 267 1696 187 2869
Fragments arranged by size:
2869 1696 548 267 187 120 110 78
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 3935 4947 3935
Size: 1012 4863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5875
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5875
BstXI CCAn_nnnn'nTGG
Cuts at: 2710 2710
Size: 5875
BstYI r'GATC_y
Cuts at: 1972 2547 2565 2854 3205 3572 3957 3974 5426
Size: 575 18 289 351 367 385 17 1452
Cuts at: 5426 5512 5524 5870 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 575 385 367 351 346 289
86 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 2010 2112 2925 3024 2010
Size: 102 813 99 4861
Fragments arranged by size:
4861 813 102 99
BtgI C'CryG_G
Cuts at: 513 1065 2158 2401 513
Size: 552 1093 243 3987
Fragments arranged by size:
3987 1093 552 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 2137 3109 3391 4185 4205 2137
Size: 972 282 794 20 3807
Fragments arranged by size:
3807 972 794 282 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DraIII CAC_nnn'GTG
Cuts at: 2698 2698
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2365 2830
Size: 54 1425 50 275 18 201 334 465
Cuts at: 2830 3106 3322 4211 5840 8
Size: 276 216 889 1629 43
Fragments arranged by size:
1629 1425 889 465 334 276 275 216
201 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2365 3322 1537
Size: 275 553 957 4090
Fragments arranged by size:
4090 957 553 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3805 1353
Size: 2452 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2345 4462 4836 4982 387
Size: 1064 138 756 2117 374 146 1280
Fragments arranged by size:
2117 1280 1064 756 374 146 138
EcoICRI GAG'CTC
Cuts at: 2811 2811
Size: 5875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2582 2618 2702 3023 3938 4535
Size: 393 504 446 36 84 321 915 597
Cuts at: 4535 5322 5590 1239
Size: 787 268 1524
Fragments arranged by size:
1524 915 787 597 504 446 393 321
268 84 36
EcoNI CCTnn'n_nnAGG
Cuts at: 3041 3041
Size: 5875
EcoO109I rG'GnC_Cy
Cuts at: 2014 2015 2199 2374 2406 2494 2602 2962 3046
Size: 1 184 175 32 88 108 360 84
Cuts at: 3046 3162 2014
Size: 116 4727
Fragments arranged by size:
4727 360 184 175 116 108 88 84
32 1
EcoRI G'AATT_C
Cuts at: 3184 3562 3184
Size: 378 5497
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 2066 5022 1678
Size: 388 2956 2531
Fragments arranged by size:
2956 2531 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3128 3160 4516 4548 4590
Size: 32 135 32 1556 32 1356 32 42
Cuts at: 4590 4622 1373
Size: 32 2626
Fragments arranged by size:
2626 1556 1356 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 3200 3200
Size: 5875
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4989 5173 1305
Size: 3684 184 2007
Fragments arranged by size:
3684 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2033 3108 10
Size: 54 1425 343 201 1075 2777
Fragments arranged by size:
2777 1425 1075 343 201 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3833 4192 4351 518
Size: 648 2667 359 159 2042
Fragments arranged by size:
2667 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2160 2263 2370 2403 2917 3019 3291 3968
Size: 554 103 107 33 514 102 272 677
Cuts at: 3968 5116 5361 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 554 514 272 245 107
103 102 33
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5323
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 2248 2800 3233 4778 1132
Size: 97 1019 552 433 1545 2229
Fragments arranged by size:
2229 1545 1019 552 433 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5875
PfoI T'CCnGG_A
Cuts at: 2499 2595 2857 2969 2499
Size: 96 262 112 5405
Fragments arranged by size:
5405 262 112 96
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2374 3046 3162 2374
Size: 672 116 5087
Fragments arranged by size:
5087 672 116
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PspOMI G'GGCC_C
Cuts at: 2014 2199 2405 2014
Size: 185 206 5484
Fragments arranged by size:
5484 206 185
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2417 2449 2417
Size: 32 5843
PstI C_TGCA'G
Cuts at: 830 2060 2610 2667 2820 3116 3227 830
Size: 1230 550 57 153 296 111 3478
Fragments arranged by size:
3478 1230 550 296 153 111 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 2263 2917 2263
Size: 654 5221
SacI G_AGCT'C
Cuts at: 2813 2813
Size: 5875
SacII CC_GC'GG
Cuts at: 2161 2404 2161
Size: 243 5632
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SbfI CC_TGCA'GG
Cuts at: 2060 2820 3227 2060
Size: 760 407 4708
Fragments arranged by size:
4708 760 407
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SfcI C'TryA_G
Cuts at: 826 1045 2056 2606 2663 2816 3112 3223 4358
Size: 219 1011 550 57 153 296 111 1135
Cuts at: 4358 5039 5230 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1011 681 550 296 219 191
153 111 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 3202 1872
Size: 1330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3979 4880 5142 820
Size: 197 926 2036 901 262 1553
Fragments arranged by size:
2036 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 2800 3233 1132
Size: 1668 433 3774
Fragments arranged by size:
3774 1668 433
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 2054 2471 1517
Size: 537 417 4921
Fragments arranged by size:
4921 537 417
StyI C'CwwG_G
Cuts at: 513 1065 2377 2703 2904 3147 3314 513
Size: 552 1312 326 201 243 167 3074
Fragments arranged by size:
3074 1312 552 326 243 201 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2581 2887 3902 3919 4072 4257 4702
Size: 1255 1001 306 1015 17 153 185 445
Cuts at: 4702 325
Size: 1498
Fragments arranged by size:
1498 1255 1015 1001 445 306 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4121 5833 96
Size: 274 80 33 51 1443 2144 1712 138
Fragments arranged by size:
2144 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5835 5867 5835
Size: 32 5843
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2855 2855
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 3200 1870
Size: 1330 4545
XmnI GAAnn'nnTTC
Cuts at: 4004 4004
Size: 5875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI RsrII SanDI
SapI SexAI SfiI SfoI SrfI SwaI