Restriction map for
pFN21AE4367
(Circular) (Six-base) MAPSORT of: pFN21AE4367.seq Check: 8310 from: 1 to: 5863
pFN21AE4367 5863 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3206 1937
Size: 1269 4594
Acc65I G'GTAC_C
Cuts at: 3184 3184
Size: 5863
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2994 3926 5310 5578 1632
Size: 1362 932 1384 268 1917
Fragments arranged by size:
1917 1384 1362 932 268
AfeI AGC'GCT
Cuts at: 2044 2044
Size: 5863
AflII C'TTAA_G
Cuts at: 820 1017 2117 820
Size: 197 1100 4566
Fragments arranged by size:
4566 1100 197
AflIII A'CryG_T
Cuts at: 1203 2474 2761 4762 5739 1203
Size: 1271 287 2001 977 1327
Fragments arranged by size:
2001 1327 1271 977 287
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 2024 4592 2024
Size: 2568 3295
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5863
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5863
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2473 2505 2473
Size: 32 5831
AlwNI CAG_nnn'CTG
Cuts at: 2902 2953 2972 3146 3606 5178 2902
Size: 51 19 174 460 1572 3587
Fragments arranged by size:
3587 1572 460 174 51 19
ApaLI G'TGCA_C
Cuts at: 2299 2765 3920 5076 2299
Size: 466 1155 1156 3086
Fragments arranged by size:
3086 1156 1155 466
ApoI r'AATT_y
Cuts at: 1493 2708 3172 3398 3550 5659 1493
Size: 1215 464 226 152 2109 1697
Fragments arranged by size:
2109 1697 1215 464 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2046 3188 1870
Size: 73 95 8 1142 4545
Fragments arranged by size:
4545 1142 95 73 8
BamHI G'GATC_C
Cuts at: 3193 3560 3193
Size: 367 5496
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3184 4639 618
Size: 325 217 627 1397 1455 1842
Fragments arranged by size:
1842 1455 1397 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3110 3182 1391
Size: 1719 72 4072
Fragments arranged by size:
4072 1719 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2122 928
Size: 971 223 4669
Fragments arranged by size:
4669 971 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4054 4088 1903
Size: 34 2117 34 3678
Fragments arranged by size:
3678 2117 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3755 4965 3755
Size: 1210 4653
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4474 136
Size: 107 122 71 631 605 2802 1525
Fragments arranged by size:
2802 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5858 5858
Size: 5863
BlpI GC'TnA_GC
Cuts at: 3280 3280
Size: 5863
Bme1580I G_kGCm'C
Cuts at: 1790 2303 2769 3924 5080 1790
Size: 513 466 1155 1156 2573
Fragments arranged by size:
2573 1156 1155 513 466
BmgBI CAC'GTC
Cuts at: 1318 2475 1318
Size: 1157 4706
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2580 3594 4552
Size: 416 53 83 399 24 1163 1014 958
Cuts at: 4552 442
Size: 1753
Fragments arranged by size:
1753 1163 1014 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3160 3160
Size: 5863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2304 4523 1239
Size: 1065 2219 2579
Fragments arranged by size:
2579 2219 1065
Bpu10I CC'TnA_GC
Cuts at: 2737 2737
Size: 5863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2222 3988 4853 5151 2222
Size: 1766 865 298 2934
Fragments arranged by size:
2934 1766 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2831 2878 4526 882
Size: 617 1332 47 1648 2219
Fragments arranged by size:
2219 1648 1332 617 47
BsaAI yAC'GTr
Cuts at: 493 1204 3654 493
Size: 711 2450 2702
Fragments arranged by size:
2702 2450 711
BsaBI GATnn'nnATC
Cuts at: 5857 5857
Size: 5863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3670 4052 4687 275
Size: 53 83 186 936 2137 382 635 1451
Fragments arranged by size:
2137 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4296 4694 4968 5115 5526 4296
Size: 398 274 147 411 4633
Fragments arranged by size:
4633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2082 2112 2234 2264 3005
Size: 30 165 30 679 30 122 30 741
Cuts at: 3005 3035 1178
Size: 30 4006
Fragments arranged by size:
4006 741 679 165 122 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 2261 2606 2763 2954 3001 3004 1186
Size: 1072 3 345 157 191 47 3 4045
Fragments arranged by size:
4045 1072 345 191 157 47 3 3
BseYI C'CCAG_C
Cuts at: 2199 2581 5066 5562 2199
Size: 382 2485 496 2500
Fragments arranged by size:
2500 2485 496 382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 3313 4074 5102 1540
Size: 275 186 33 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2303 2769 3924 4009 5080 2303
Size: 466 1155 85 1071 3086
Fragments arranged by size:
3086 1155 1071 466 85
BsmI GAATG_Cn'
Cuts at: 3374 3467 3374
Size: 93 5770
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2303 2769 3110 3182 3924 4009 5080
Size: 399 513 466 341 72 742 85 1071
Cuts at: 5080 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 513 466 399 341 85
72
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 3752 5482 3752
Size: 1730 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3218 844
Size: 2374 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3750 1394
Size: 429 1927 3507
Fragments arranged by size:
3507 1927 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2322 4358 4532 65
Size: 1183 1074 2036 174 1396
Fragments arranged by size:
2036 1396 1183 1074 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2152 4507 4694 1700
Size: 110 342 2355 187 2869
Fragments arranged by size:
2869 2355 342 187 110
BsrGI T'GTAC_A
Cuts at: 96 2357 96
Size: 2261 3602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 2802 3923 4935 2802
Size: 1121 1012 3730
Fragments arranged by size:
3730 1121 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3146 794
Size: 2352 3511
BstBI TT'CG_AA
Cuts at: 4667 4667
Size: 5863
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5863
BstXI CCAn_nnnn'nTGG
Cuts at: 2500 2611 2500
Size: 111 5752
BstYI r'GATC_y
Cuts at: 1972 2112 2261 3193 3560 3945 3962 5414 5500
Size: 140 149 932 367 385 17 1452 86
Cuts at: 5500 5512 5858 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 932 385 367 346 149 140
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2093 513
Size: 552 939 89 4283
Fragments arranged by size:
4283 939 552 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5124
Fragments arranged by size:
5124 683 56
BtsI GCAGTG_nn'
Cuts at: 3379 4173 4193 3379
Size: 794 20 5049
Fragments arranged by size:
5049 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 3167 3167
Size: 5863
DraIII CAC_nnn'GTG
Cuts at: 2418 2418
Size: 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2929 4870 809
Size: 507 1613 1941 1802
Fragments arranged by size:
1941 1802 1613 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3310 4199 5828
Size: 54 1425 50 275 18 1480 889 1629
Cuts at: 5828 8
Size: 43
Fragments arranged by size:
1629 1480 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3310 1537
Size: 275 1498 4090
Fragments arranged by size:
4090 1498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3793 1353
Size: 2440 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2831 4450 4824 4970 387
Size: 1064 138 1242 1619 374 146 1280
Fragments arranged by size:
1619 1280 1242 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2304 2994 3926 4523 5310 5578 1239
Size: 393 672 690 932 597 787 268 1524
Fragments arranged by size:
1524 932 787 690 672 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2240 2240
Size: 5863
EcoO109I rG'GnC_Cy
Cuts at: 2753 2753
Size: 5863
EcoRI G'AATT_C
Cuts at: 3172 3550 3172
Size: 378 5485
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 2046 2059 5010 1678
Size: 368 13 2951 2531
Fragments arranged by size:
2951 2531 368 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2010 2042 2082 2114 2438
Size: 32 135 32 438 32 40 32 324
Cuts at: 2438 2470 2487 2519 3005 3037 4504 4536 4578
Size: 32 17 32 486 32 1467 32 42
Cuts at: 4578 4610 1373
Size: 32 2626
Fragments arranged by size:
2626 1467 486 438 324 135 42 40
32 32 32 32 32 32 32 32
32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 3207 3453 669
Size: 988 281 226 1043 246 3079
Fragments arranged by size:
3079 1043 988 281 246 226
HindIII A'AGCT_T
Cuts at: 748 2437 748
Size: 1689 4174
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KpnI G_GTAC'C
Cuts at: 3188 3188
Size: 5863
MfeI C'AATT_G
Cuts at: 3462 3462
Size: 5863
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2355 2681 4977 5161 1305
Size: 1050 326 2296 184 2007
Fragments arranged by size:
2296 2007 1050 326 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4041
Fragments arranged by size:
4041 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2651 3821 4180 4339 518
Size: 648 1485 1170 359 159 2042
Fragments arranged by size:
2042 1485 1170 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2558 2588 3026 3279 3956 5104 5349 1606
Size: 952 30 438 253 677 1148 245 2120
Fragments arranged by size:
2120 1148 952 677 438 253 245 30
NcoI C'CATG_G
Cuts at: 513 1065 2004 2093 513
Size: 552 939 89 4283
Fragments arranged by size:
4283 939 552 89
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5863
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 3077 3221 4766 1132
Size: 97 1848 144 1545 2229
Fragments arranged by size:
2229 1848 1545 144 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5863
PciI A'CATG_T
Cuts at: 4762 4762
Size: 5863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2094 3103 1108
Size: 986 1009 3868
Fragments arranged by size:
3868 1009 986
PfoI T'CCnGG_A
Cuts at: 2062 2100 2442 2062
Size: 38 342 5483
Fragments arranged by size:
5483 342 38
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 5445 5477 3932
Size: 32 1481 32 4318
Fragments arranged by size:
4318 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2753 2753
Size: 5863
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5863
PsiI TTA'TAA
Cuts at: 3433 3433
Size: 5863
PspXI vC'TCGA_Gb
Cuts at: 2038 2046 2038
Size: 8 5855
PstI C_TGCA'G
Cuts at: 830 3215 830
Size: 2385 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
PvuII CAG'CTG
Cuts at: 2558 2558
Size: 5863
RsrII CG'GwC_CG
Cuts at: 2155 2155
Size: 5863
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 4111 5823 4111
Size: 1712 4151
SfcI C'TryA_G
Cuts at: 826 1045 2925 3211 4346 5027 5218 826
Size: 219 1880 286 1135 681 191 1471
Fragments arranged by size:
1880 1471 1135 681 286 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 3190 1872
Size: 1318 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2038 2046 2117 2237 3967 4868
Size: 197 926 95 8 71 120 1730 901
Cuts at: 4868 5130 820
Size: 262 1553
Fragments arranged by size:
1730 1553 926 901 262 197 120 95
71 8
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 3221 1132
Size: 2089 3774
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5863
StyI C'CwwG_G
Cuts at: 513 1065 2004 2093 2890 3302 513
Size: 552 939 89 797 412 3074
Fragments arranged by size:
3074 939 797 552 412 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2882 3890 3907 4060 4245 4690 325
Size: 1255 1302 1008 17 153 185 445 1498
Fragments arranged by size:
1498 1302 1255 1008 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2357 4109 5821
Size: 274 80 33 51 1443 380 1752 1712
Cuts at: 5821 96
Size: 138
Fragments arranged by size:
1752 1712 1443 380 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2234 2266 5823 5855 2234
Size: 32 3557 32 2242
Fragments arranged by size:
3557 2242 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2077 1316
Size: 761 5102
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XhoI C'TCGA_G
Cuts at: 1943 2038 2046 1943
Size: 95 8 5760
Fragments arranged by size:
5760 95 8
XmaI C'CCGG_G
Cuts at: 1870 3188 1870
Size: 1318 4545
XmnI GAAnn'nnTTC
Cuts at: 2708 3087 3992 2708
Size: 379 905 4579
Fragments arranged by size:
4579 905 379
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PsrI SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI