Restriction map for
pFN21AE4366
(Circular) (Six-base) MAPSORT of: pFN21AE4366.seq Check: 2423 from: 1 to: 5854
pFN21AE4366 5854 bp
With 182 enzymes: SgfI *
March 28, 2012 13:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3664 278
Size: 53 83 186 936 2128 2468
Fragments arranged by size:
2468 2128 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3197 1937
Size: 1260 4594
Acc65I G'GTAC_C
Cuts at: 3175 3175
Size: 5854
AclI AA'CG_TT
Cuts at: 1563 3981 4354 1563
Size: 2418 373 3063
Fragments arranged by size:
3063 2418 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 3047 3917 5301 5569 1632
Size: 662 753 870 1384 268 1917
Fragments arranged by size:
1917 1384 870 753 662 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5657
AflIII A'CryG_T
Cuts at: 1203 2733 4753 5730 1203
Size: 1530 2020 977 1327
Fragments arranged by size:
2020 1530 1327 977
AgeI A'CCGG_T
Cuts at: 4685 4685
Size: 5854
AhdI GACnn_n'nnGTC
Cuts at: 4583 4583
Size: 5854
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5854
AlwNI CAG_nnn'CTG
Cuts at: 2521 3025 3106 3597 5169 2521
Size: 504 81 491 1572 3206
Fragments arranged by size:
3206 1572 504 491 81
ApaI G_GGCC'C
Cuts at: 3064 3064
Size: 5854
ApaLI G'TGCA_C
Cuts at: 3911 5067 3911
Size: 1156 4698
ApoI r'AATT_y
Cuts at: 1493 2483 2850 3163 3389 3541 5650 1493
Size: 990 367 313 226 152 2109 1697
Fragments arranged by size:
2109 1697 990 367 313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4408 160
Size: 872 3376 1606
Fragments arranged by size:
3376 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5854
AvaI C'yCGr_G
Cuts at: 1870 1943 3179 1870
Size: 73 1236 4545
Fragments arranged by size:
4545 1236 73
BamHI G'GATC_C
Cuts at: 3184 3551 3184
Size: 367 5487
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3047 3175 4630 618
Size: 325 217 627 1260 128 1455 1842
Fragments arranged by size:
1842 1455 1260 627 325 217 128
BanII G_rGCy'C
Cuts at: 1391 2752 2833 3064 3089 3173 1391
Size: 1361 81 231 25 84 4072
Fragments arranged by size:
4072 1361 231 84 81 25
BbeI G_GCGC'C
Cuts at: 3051 3051
Size: 5854
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 928
Size: 971 383 4500
Fragments arranged by size:
4500 971 383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4045 4079 1903
Size: 34 2108 34 3678
Fragments arranged by size:
3678 2108 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2978 3746 4956 2978
Size: 768 1210 3876
Fragments arranged by size:
3876 1210 768
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5854
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4465 136
Size: 107 122 71 631 605 2793 1525
Fragments arranged by size:
2793 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2898 5849 2898
Size: 2951 2903
BlpI GC'TnA_GC
Cuts at: 2525 3271 2525
Size: 746 5108
Bme1580I G_kGCm'C
Cuts at: 1790 3064 3915 5071 1790
Size: 1274 851 1156 2573
Fragments arranged by size:
2573 1274 1156 851
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5854
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2095 3585 4543
Size: 416 53 83 399 24 678 1490 958
Cuts at: 4543 442
Size: 1753
Fragments arranged by size:
1753 1490 958 678 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5854
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2817 2817
Size: 5854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 4514 1239
Size: 797 2478 2579
Fragments arranged by size:
2579 2478 797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 3979 4844 5142 2237
Size: 1742 865 298 2949
Fragments arranged by size:
2949 1742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2316 4517 882
Size: 617 817 2201 2219
Fragments arranged by size:
2219 2201 817 617
BsaAI yAC'GTr
Cuts at: 493 1204 3645 493
Size: 711 2441 2702
Fragments arranged by size:
2702 2441 711
BsaBI GATnn'nnATC
Cuts at: 2774 5848 2774
Size: 3074 2780
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 3048 3661 4043
Size: 53 83 186 936 485 1030 613 382
Cuts at: 4043 4678 275
Size: 635 1451
Fragments arranged by size:
1451 1030 936 635 613 485 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4287 4685 4959 5106 5517 4287
Size: 398 274 147 411 4624
Fragments arranged by size:
4624 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2910 2940 1178
Size: 30 165 30 1507 30 4092
Fragments arranged by size:
4092 1507 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2806 1186
Size: 1620 4234
BseYI C'CCAG_C
Cuts at: 2499 2743 5057 5553 2499
Size: 244 2314 496 2800
Fragments arranged by size:
2800 2314 496 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3038 1163
Size: 573 1302 3979
Fragments arranged by size:
3979 1302 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3304 4065 5093 1540
Size: 275 186 1303 761 1028 2301
Fragments arranged by size:
2301 1303 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3915 4000 5071 3915
Size: 85 1071 4698
Fragments arranged by size:
4698 1071 85
BsmI GAATG_Cn'
Cuts at: 3365 3458 3365
Size: 93 5761
BsmBI CGTCTCn'nnnn_
Cuts at: 2025 2025
Size: 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2752 2833 3064 3089 3173 3915 4000
Size: 399 962 81 231 25 84 742 85
Cuts at: 4000 5071 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 962 742 399 231 85 84
81 25
BspEI T'CCGG_A
Cuts at: 5517 5517
Size: 5854
BspHI T'CATG_A
Cuts at: 2613 3743 5473 2613
Size: 1130 1730 2994
Fragments arranged by size:
2994 1730 1130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2938 3209 844
Size: 2094 271 3489
Fragments arranged by size:
3489 2094 271
BsrBI CCG'CTC
Cuts at: 1394 1823 3741 1394
Size: 429 1918 3507
Fragments arranged by size:
3507 1918 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2574 4349 4523 65
Size: 1183 1326 1775 174 1396
Fragments arranged by size:
1775 1396 1326 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2859 4498 4685 1700
Size: 110 1049 1639 187 2869
Fragments arranged by size:
2869 1639 1049 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5854
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5854
BssSI C'ACGA_G
Cuts at: 3914 4926 3914
Size: 1012 4842
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2521 2540 3146 794
Size: 1727 19 606 3502
Fragments arranged by size:
3502 1727 606 19
BstBI TT'CG_AA
Cuts at: 4658 4658
Size: 5854
BstEII G'GTnAC_C
Cuts at: 1184 2551 1184
Size: 1367 4487
BstXI CCAn_nnnn'nTGG
Cuts at: 2764 2764
Size: 5854
BstYI r'GATC_y
Cuts at: 1972 2898 3103 3184 3551 3936 3953 5405 5491
Size: 926 205 81 367 385 17 1452 86
Cuts at: 5491 5503 5849 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 926 385 367 346 205 86
81 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2392 2914 3051 513
Size: 552 939 388 522 137 3316
Fragments arranged by size:
3316 939 552 522 388 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2130 487
Size: 683 56 904 4211
Fragments arranged by size:
4211 904 683 56
BtsI GCAGTG_nn'
Cuts at: 3370 4164 4184 3370
Size: 794 20 5040
Fragments arranged by size:
5040 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5854
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5819
DraI TTT'AAA
Cuts at: 3158 3158
Size: 5854
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2284 4861 809
Size: 507 968 2577 1802
Fragments arranged by size:
2577 1802 968 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2941 3097 3301
Size: 54 1425 50 275 18 1111 156 204
Cuts at: 3301 4190 5819 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1111 889 275 204 156 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3301 1537
Size: 275 1489 4090
Fragments arranged by size:
4090 1489 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3784 1353
Size: 2431 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4441 4815 4961 387
Size: 1064 138 2852 374 146 1280
Fragments arranged by size:
2852 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2294 3047 3917 4514 5301 5569
Size: 393 404 258 753 870 597 787 268
Cuts at: 5569 1239
Size: 1524
Fragments arranged by size:
1524 870 787 753 597 404 393 268
258
EcoNI CCTnn'n_nnAGG
Cuts at: 2465 2788 2465
Size: 323 5531
EcoO109I rG'GnC_Cy
Cuts at: 2880 2950 3061 2880
Size: 70 111 5673
Fragments arranged by size:
5673 111 70
EcoRI G'AATT_C
Cuts at: 2850 3163 3541 2850
Size: 313 378 5163
Fragments arranged by size:
5163 378 313
EcoRV GAT'ATC
Cuts at: 2586 2586
Size: 5854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 5854
FspI TGC'GCA
Cuts at: 2387 4360 2387
Size: 1973 3881
HaeII r_GCGC'y
Cuts at: 1678 3051 5001 1678
Size: 1373 1950 2531
Fragments arranged by size:
2531 1950 1373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2820 2852 2910 2942 4495
Size: 32 135 32 1248 32 58 32 1553
Cuts at: 4495 4527 4569 4601 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1553 1248 135 58 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3198 3444 669
Size: 988 281 1260 246 3079
Fragments arranged by size:
3079 1260 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5854
HpaI GTT'AAC
Cuts at: 3444 3444
Size: 5854
KasI G'GCGC_C
Cuts at: 3047 3047
Size: 5854
KpnI G_GTAC'C
Cuts at: 3179 3179
Size: 5854
MfeI C'AATT_G
Cuts at: 3453 3453
Size: 5854
MluI A'CGCG_T
Cuts at: 5730 5730
Size: 5854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2272 2361 2806 4968 5152 1305
Size: 967 89 445 2162 184 2007
Fragments arranged by size:
2162 2007 967 445 184 89
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4032
Fragments arranged by size:
4032 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3812 4171 4330 518
Size: 648 843 1803 359 159 2042
Fragments arranged by size:
2042 1803 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2094 2916 3040 3270 3947 5095 5340 1606
Size: 488 822 124 230 677 1148 245 2120
Fragments arranged by size:
2120 1148 822 677 488 245 230 124
NarI GG'CG_CC
Cuts at: 3048 3048
Size: 5854
NcoI C'CATG_G
Cuts at: 513 1065 2004 2392 3051 513
Size: 552 939 388 659 3316
Fragments arranged by size:
3316 939 659 552 388
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5854
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5854
NotI GC'GGCC_GC
Cuts at: 3301 3301
Size: 5854
NspI r_CATG'y
Cuts at: 1132 1229 2242 2737 3212 4757 1132
Size: 97 1013 495 475 1545 2229
Fragments arranged by size:
2229 1545 1013 495 475 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5854
PciI A'CATG_T
Cuts at: 2733 4753 2733
Size: 2020 3834
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2596 3057 1108
Size: 1488 461 3905
Fragments arranged by size:
3905 1488 461
PmeI GTTT'AAAC
Cuts at: 3158 3158
Size: 5854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3923 3955 5436 5468 3923
Size: 32 1481 32 4309
Fragments arranged by size:
4309 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2880 2950 2880
Size: 70 5784
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5854
PsiI TTA'TAA
Cuts at: 2489 3424 2489
Size: 935 4919
PspOMI G'GGCC_C
Cuts at: 3060 3060
Size: 5854
PstI C_TGCA'G
Cuts at: 830 3206 830
Size: 2376 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5854
PvuII CAG'CTG
Cuts at: 2094 3040 2094
Size: 946 4908
SacII CC_GC'GG
Cuts at: 2917 2917
Size: 5854
SalI G'TCGA_C
Cuts at: 1936 3196 1936
Size: 1260 4594
SanDI GG'GwC_CC
Cuts at: 2880 2880
Size: 5854
SbfI CC_TGCA'GG
Cuts at: 3206 3206
Size: 5854
ScaI AGT'ACT
Cuts at: 4102 5814 4102
Size: 1712 4142
SfcI C'TryA_G
Cuts at: 826 1045 2170 3202 4337 5018 5209 826
Size: 219 1125 1032 1135 681 191 1471
Fragments arranged by size:
1471 1135 1125 1032 681 219 191
SfoI GGC'GCC
Cuts at: 3049 3049
Size: 5854
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5854
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5854
SmaI CCC'GGG
Cuts at: 1872 3181 1872
Size: 1309 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 3958 4859 5121 820
Size: 197 926 309 1706 901 262 1553
Fragments arranged by size:
1706 1553 926 901 309 262 197
SnaBI TAC'GTA
Cuts at: 493 3645 493
Size: 3152 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5854
SphI G_CATG'C
Cuts at: 1132 2242 3212 1132
Size: 1110 970 3774
Fragments arranged by size:
3774 1110 970
SspI AAT'ATT
Cuts at: 5 52 3778 5
Size: 47 3726 2081
Fragments arranged by size:
3726 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5854
StyI C'CwwG_G
Cuts at: 513 1065 2004 2392 2868 2964 3051 3293 513
Size: 552 939 388 476 96 87 242 3074
Fragments arranged by size:
3074 939 552 476 388 242 96 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2357 2405 3881 3898 4051 4236
Size: 1255 417 360 48 1476 17 153 185
Cuts at: 4236 4681 325
Size: 445 1498
Fragments arranged by size:
1498 1476 1255 445 417 360 185 153
48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4100 5812 96
Size: 274 80 33 51 1443 2123 1712 138
Fragments arranged by size:
2123 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5814 5846 5814
Size: 32 5822
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5854
XbaI T'CTAG_A
Cuts at: 3190 3190
Size: 5854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2399 2399
Size: 5854
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5854
XmaI C'CCGG_G
Cuts at: 1870 3179 1870
Size: 1309 4545
XmnI GAAnn'nnTTC
Cuts at: 3983 3983
Size: 5854
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3662 276
Size: 53 83 186 936 2128 2468
Fragments arranged by size:
2468 2128 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI