Restriction map for
pFN21AE4365
(Circular) (Six-base) MAPSORT of: pFN21AE4365.seq Check: 5532 from: 1 to: 5854
pFN21AE4365 5854 bp
With 182 enzymes: SgfI *
March 28, 2012 13:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2124 2441 2678 2124
Size: 317 237 5300
Fragments arranged by size:
5300 317 237
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3664 278
Size: 53 83 186 936 2128 2468
Fragments arranged by size:
2468 2128 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3197 1937
Size: 1260 4594
Acc65I G'GTAC_C
Cuts at: 3175 3175
Size: 5854
AclI AA'CG_TT
Cuts at: 1563 3981 4354 1563
Size: 2418 373 3063
Fragments arranged by size:
3063 2418 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2879 3041 3109 3917 5301 5569 1632
Size: 1247 162 68 808 1384 268 1917
Fragments arranged by size:
1917 1384 1247 808 268 162 68
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5657
AflIII A'CryG_T
Cuts at: 1203 4753 5730 1203
Size: 3550 977 1327
Fragments arranged by size:
3550 1327 977
AgeI A'CCGG_T
Cuts at: 4685 4685
Size: 5854
AhdI GACnn_n'nnGTC
Cuts at: 4583 4583
Size: 5854
AleI CACnn'nnGTG
Cuts at: 1166 2435 2687 1166
Size: 1269 252 4333
Fragments arranged by size:
4333 1269 252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2690 1184
Size: 1506 4348
AlwNI CAG_nnn'CTG
Cuts at: 3597 5169 3597
Size: 1572 4282
ApaI G_GGCC'C
Cuts at: 2394 2394
Size: 5854
ApaLI G'TGCA_C
Cuts at: 2819 3911 5067 2819
Size: 1092 1156 3606
Fragments arranged by size:
3606 1156 1092
ApoI r'AATT_y
Cuts at: 1493 3163 3389 3541 5650 1493
Size: 1670 226 152 2109 1697
Fragments arranged by size:
2109 1697 1670 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4408 160
Size: 872 3376 1606
Fragments arranged by size:
3376 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5854
AvaI C'yCGr_G
Cuts at: 1870 1943 2216 2354 2362 2714 3101 3179 1870
Size: 73 273 138 8 352 387 78 4545
Fragments arranged by size:
4545 387 352 273 138 78 73 8
BamHI G'GATC_C
Cuts at: 3184 3551 3184
Size: 367 5487
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2136 2429 2589 2666 3175
Size: 325 217 627 349 293 160 77 509
Cuts at: 3175 4630 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 509 349 325 293 217
160 77
BanII G_rGCy'C
Cuts at: 1391 2256 2394 2759 2880 3173 1391
Size: 865 138 365 121 293 4072
Fragments arranged by size:
4072 865 365 293 138 121
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2971 928
Size: 971 1072 3811
Fragments arranged by size:
3811 1072 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2548 2582 4045 4079 1903
Size: 34 611 34 1463 34 3678
Fragments arranged by size:
3678 1463 611 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3746 4956 3746
Size: 1210 4644
BclI T'GATC_A
Cuts at: 1548 2400 1548
Size: 852 5002
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2145 2149 2484
Size: 107 122 71 631 605 473 4 335
Cuts at: 2484 2598 4465 136
Size: 114 1867 1525
Fragments arranged by size:
1867 1525 631 605 473 335 122 114
107 71 4
BglII A'GATC_T
Cuts at: 5849 5849
Size: 5854
BlpI GC'TnA_GC
Cuts at: 2753 3271 2753
Size: 518 5336
Bme1580I G_kGCm'C
Cuts at: 1790 2353 2394 2480 2594 2669 2823 2966 3915
Size: 563 41 86 114 75 154 143 949
Cuts at: 3915 5071 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 949 563 154 143 114 86
75 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5854
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3585 4543 442
Size: 416 53 83 399 24 2168 958 1753
Fragments arranged by size:
2168 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4514 1239
Size: 3275 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3979 4844 5142 3979
Size: 865 298 4691
Fragments arranged by size:
4691 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2732 2990 4517 882
Size: 617 1233 258 1527 2219
Fragments arranged by size:
2219 1527 1233 617 258
BsaAI yAC'GTr
Cuts at: 493 1204 3645 493
Size: 711 2441 2702
Fragments arranged by size:
2702 2441 711
BsaBI GATnn'nnATC
Cuts at: 5848 5848
Size: 5854
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2553 3661 4043 4678
Size: 53 83 186 936 1020 1108 382 635
Cuts at: 4678 275
Size: 1451
Fragments arranged by size:
1451 1108 1020 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4287 4685 4959 5106 5517 4287
Size: 398 274 147 411 4624
Fragments arranged by size:
4624 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5629
Fragments arranged by size:
5629 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2272 2888 3161 1186
Size: 1086 616 273 3879
Fragments arranged by size:
3879 1086 616 273
BseYI C'CCAG_C
Cuts at: 2241 2593 2760 2959 5057 5553 2241
Size: 352 167 199 2098 496 2542
Fragments arranged by size:
2542 2098 496 352 199 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2509 2542 2855 1163
Size: 573 773 33 313 4162
Fragments arranged by size:
4162 773 573 313 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 3304 4065 5093 1540
Size: 275 186 15 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2256 2823 2850 2880 2943 3915 4000 5071 2256
Size: 567 27 30 63 972 85 1071 3039
Fragments arranged by size:
3039 1071 972 567 85 63 30 27
BsmI GAATG_Cn'
Cuts at: 2084 2345 3365 3458 2084
Size: 261 1020 93 4480
Fragments arranged by size:
4480 1020 261 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 2353 2394 2480 2594 2669 2759
Size: 399 466 97 41 86 114 75 90
Cuts at: 2759 2823 2850 2880 2943 2966 3173 3915 4000
Size: 64 27 30 63 23 207 742 85
Cuts at: 4000 5071 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 466 399 207 114 97
90 86 85 75 64 63 41 30
27 23
BspEI T'CCGG_A
Cuts at: 5517 5517
Size: 5854
BspHI T'CATG_A
Cuts at: 3743 5473 3743
Size: 1730 4124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 2318 2441 2624 2678 2755 3021 3057
Size: 1280 194 123 183 54 77 266 36
Cuts at: 3057 3209 844
Size: 152 3489
Fragments arranged by size:
3489 1280 266 194 183 152 123 77
54 36
BsrBI CCG'CTC
Cuts at: 1394 1823 3741 1394
Size: 429 1918 3507
Fragments arranged by size:
3507 1918 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4349 4523 65
Size: 1183 3101 174 1396
Fragments arranged by size:
3101 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2586 2597 2830 3137 4498 4685 1700
Size: 110 776 11 233 307 1361 187 2869
Fragments arranged by size:
2869 1361 776 307 233 187 110 11
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5854
BssHII G'CGCG_C
Cuts at: 1925 2746 2748 1925
Size: 821 2 5031
Fragments arranged by size:
5031 821 2
BssSI C'ACGA_G
Cuts at: 2822 3914 4926 2822
Size: 1092 1012 3750
Fragments arranged by size:
3750 1092 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2502 2652 2827 794
Size: 1708 150 175 3821
Fragments arranged by size:
3821 1708 175 150
BstBI TT'CG_AA
Cuts at: 4658 4658
Size: 5854
BstEII G'GTnAC_C
Cuts at: 1184 2629 2767 1184
Size: 1445 138 4271
Fragments arranged by size:
4271 1445 138
BstXI CCAn_nnnn'nTGG
Cuts at: 2953 2953
Size: 5854
BstYI r'GATC_y
Cuts at: 1972 3184 3551 3936 3953 5405 5491 5503 5849
Size: 1212 367 385 17 1452 86 12 346
Cuts at: 5849 1972
Size: 1977
Fragments arranged by size:
1977 1452 1212 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5302
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5115
Fragments arranged by size:
5115 683 56
BtsI GCAGTG_nn'
Cuts at: 2228 3370 4164 4184 2228
Size: 1142 794 20 3898
Fragments arranged by size:
3898 1142 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5854
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5819
DraI TTT'AAA
Cuts at: 3158 3158
Size: 5854
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3004 3083 4861 809
Size: 507 1688 79 1778 1802
Fragments arranged by size:
1802 1778 1688 507 79
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2056 2514 3081
Size: 54 1425 50 275 18 226 458 567
Cuts at: 3081 3301 4190 5819 8
Size: 220 889 1629 43
Fragments arranged by size:
1629 1425 889 567 458 275 226 220
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3301 1537
Size: 275 1489 4090
Fragments arranged by size:
4090 1489 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3784 1353
Size: 2431 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4441 4815 4961 387
Size: 1064 138 2852 374 146 1280
Fragments arranged by size:
2852 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2254 2878 2254
Size: 624 5230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2879 3041 3109 3917 4514 5301 5569
Size: 393 1247 162 68 808 597 787 268
Cuts at: 5569 1239
Size: 1524
Fragments arranged by size:
1524 1247 808 787 597 393 268 162
68
EcoNI CCTnn'n_nnAGG
Cuts at: 2903 2903
Size: 5854
EcoO109I rG'GnC_Cy
Cuts at: 2156 2307 2391 3076 2156
Size: 151 84 685 4934
Fragments arranged by size:
4934 685 151 84
EcoRI G'AATT_C
Cuts at: 3163 3541 3163
Size: 378 5476
FspI TGC'GCA
Cuts at: 4360 4360
Size: 5854
HaeII r_GCGC'y
Cuts at: 1678 5001 1678
Size: 3323 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4495 4527 4569 4601 1373
Size: 32 135 32 2923 32 42 32 2626
Fragments arranged by size:
2923 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3088 3198 3444 669
Size: 988 281 1150 110 246 3079
Fragments arranged by size:
3079 1150 988 281 246 110
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5854
HpaI GTT'AAC
Cuts at: 3444 3444
Size: 5854
KpnI G_GTAC'C
Cuts at: 3179 3179
Size: 5854
MfeI C'AATT_G
Cuts at: 3453 3453
Size: 5854
MluI A'CGCG_T
Cuts at: 5730 5730
Size: 5854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3033 4968 5152 1305
Size: 1728 1935 184 2007
Fragments arranged by size:
2007 1935 1728 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2516 10
Size: 54 1425 343 684 3348
Fragments arranged by size:
3348 1425 684 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 2687 3812 4171 4330 518
Size: 648 1269 252 1125 359 159 2042
Fragments arranged by size:
2042 1269 1125 648 359 252 159
MspA1I CmG'CkG
Cuts at: 1606 2523 2583 3069 3270 3947 5095 5340 1606
Size: 917 60 486 201 677 1148 245 2120
Fragments arranged by size:
2120 1148 917 677 486 245 201 60
NaeI GCC'GGC
Cuts at: 2599 3139 2599
Size: 540 5314
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5302
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5854
NgoMIV G'CCGG_C
Cuts at: 2597 3137 2597
Size: 540 5314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5854
NotI GC'GGCC_GC
Cuts at: 3301 3301
Size: 5854
NspI r_CATG'y
Cuts at: 1132 1229 3031 3212 4757 1132
Size: 97 1802 181 1545 2229
Fragments arranged by size:
2229 1802 1545 181 97
PasI CC'CwG_GG
Cuts at: 1348 2813 1348
Size: 1465 4389
PciI A'CATG_T
Cuts at: 4753 4753
Size: 5854
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5854
PmeI GTTT'AAAC
Cuts at: 3158 3158
Size: 5854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3923 3955 5436 5468 3923
Size: 32 1481 32 4309
Fragments arranged by size:
4309 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2156 2307 3076 2156
Size: 151 769 4934
Fragments arranged by size:
4934 769 151
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5854
PsiI TTA'TAA
Cuts at: 3424 3424
Size: 5854
PspOMI G'GGCC_C
Cuts at: 2390 2390
Size: 5854
PstI C_TGCA'G
Cuts at: 830 2621 2679 3206 830
Size: 1791 58 527 3478
Fragments arranged by size:
3478 1791 527 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5854
PvuII CAG'CTG
Cuts at: 2523 2523
Size: 5854
SacI G_AGCT'C
Cuts at: 2256 2880 2256
Size: 624 5230
SalI G'TCGA_C
Cuts at: 1936 3196 1936
Size: 1260 4594
SanDI GG'GwC_CC
Cuts at: 2156 2156
Size: 5854
SbfI CC_TGCA'GG
Cuts at: 3206 3206
Size: 5854
ScaI AGT'ACT
Cuts at: 4102 5814 4102
Size: 1712 4142
SfcI C'TryA_G
Cuts at: 826 1045 2617 2675 3202 4337 5018 5209 826
Size: 219 1572 58 527 1135 681 191 1471
Fragments arranged by size:
1572 1471 1135 681 527 219 191 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5854
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5854
SmaI CCC'GGG
Cuts at: 1872 2218 2356 3181 1872
Size: 346 138 825 4545
Fragments arranged by size:
4545 825 346 138
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3958 4859 5121 820
Size: 197 926 2015 901 262 1553
Fragments arranged by size:
2015 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3645 493
Size: 3152 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5854
SphI G_CATG'C
Cuts at: 1132 3212 1132
Size: 2080 3774
SspI AAT'ATT
Cuts at: 5 52 3778 5
Size: 47 3726 2081
Fragments arranged by size:
3726 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5854
StyI C'CwwG_G
Cuts at: 513 1065 3293 513
Size: 552 2228 3074
Fragments arranged by size:
3074 2228 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 2699 2992 3881 3898 4051 4236
Size: 1255 450 669 293 889 17 153 185
Cuts at: 4236 4681 325
Size: 445 1498
Fragments arranged by size:
1498 1255 889 669 450 445 293 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3060 4100 5812
Size: 274 80 33 51 1443 1083 1040 1712
Cuts at: 5812 96
Size: 138
Fragments arranged by size:
1712 1443 1083 1040 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5814 5846 5814
Size: 32 5822
Tth111I GACn'n_nGTC
Cuts at: 1316 2552 1316
Size: 1236 4618
XbaI T'CTAG_A
Cuts at: 3190 3190
Size: 5854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2066 2066
Size: 5854
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5854
XmaI C'CCGG_G
Cuts at: 1870 2216 2354 3179 1870
Size: 346 138 825 4545
Fragments arranged by size:
4545 825 346 138
XmnI GAAnn'nnTTC
Cuts at: 3983 3983
Size: 5854
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3662 276
Size: 53 83 186 936 2128 2468
Fragments arranged by size:
2468 2128 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI