Restriction map for
pFN21AE4364
(Circular) (Six-base) MAPSORT of: pFN21AE4364.seq Check: 8881 from: 1 to: 5848
pFN21AE4364 5848 bp
With 182 enzymes: SgfI *
March 28, 2012 13:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2858 2858
Size: 5848
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2163 3191 1937
Size: 226 1028 4594
Fragments arranged by size:
4594 1028 226
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2552 3911 5295 5563 1632
Size: 920 1359 1384 268 1917
Fragments arranged by size:
1917 1384 1359 920 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 2424 3009 4747 5724 1203
Size: 1221 585 1738 977 1327
Fragments arranged by size:
1738 1327 1221 977 585
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2891 1184
Size: 1707 4141
AlwNI CAG_nnn'CTG
Cuts at: 2833 3591 5163 2833
Size: 758 1572 3518
Fragments arranged by size:
3518 1572 758
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 3157 3383 3535 5644 1493
Size: 1664 226 152 2109 1697
Fragments arranged by size:
2109 1697 1664 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 3173 1870
Size: 73 1230 4545
Fragments arranged by size:
4545 1230 73
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3169 4624 618
Size: 325 217 627 1382 1455 1842
Fragments arranged by size:
1842 1455 1382 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2999 3035 3167 1391
Size: 1608 36 132 4072
Fragments arranged by size:
4072 1608 132 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BbvCI CC'TCA_GC
Cuts at: 2072 2072
Size: 5848
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4039 4073 1903
Size: 34 50 34 2018 34 3678
Fragments arranged by size:
3678 2018 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4950 3740
Size: 1210 4638
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2298 2502 4459
Size: 107 122 71 631 605 626 204 1957
Cuts at: 4459 136
Size: 1525
Fragments arranged by size:
1957 1525 631 626 605 204 122 107
71
BglII A'GATC_T
Cuts at: 2790 5843 2790
Size: 3053 2795
BlpI GC'TnA_GC
Cuts at: 2384 3265 2384
Size: 881 4967
Bme1580I G_kGCm'C
Cuts at: 1790 2870 3909 5065 1790
Size: 1080 1039 1156 2573
Fragments arranged by size:
2573 1156 1080 1039
BmgBI CAC'GTC
Cuts at: 1318 3010 1318
Size: 1692 4156
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2880 3579 4537
Size: 416 53 83 399 24 1463 699 958
Cuts at: 4537 442
Size: 1753
Fragments arranged by size:
1753 1463 958 699 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4508 1239
Size: 3269 2579
Bpu10I CC'TnA_GC
Cuts at: 2072 2909 2072
Size: 837 5011
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 3973 4838 5136 2333
Size: 1640 865 298 3045
Fragments arranged by size:
3045 1640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4511 882
Size: 617 3012 2219
Fragments arranged by size:
3012 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2380 2842 3639 493
Size: 711 1176 462 797 2702
Fragments arranged by size:
2702 1176 797 711 462
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2643 3655 4037 4672
Size: 53 83 186 936 1110 1012 382 635
Cuts at: 4672 275
Size: 1451
Fragments arranged by size:
1451 1110 1012 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3086 3116 1178
Size: 30 165 30 1683 30 3910
Fragments arranged by size:
3910 1683 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2072 2255 2711 2801 2945 1186
Size: 883 3 183 456 90 144 4089
Fragments arranged by size:
4089 883 456 183 144 90 3
BseYI C'CCAG_C
Cuts at: 3028 5051 5547 3028
Size: 2023 496 3329
Fragments arranged by size:
3329 2023 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3074 1163
Size: 573 1338 3937
Fragments arranged by size:
3937 1338 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3298 4059 5087 1540
Size: 275 186 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2385 2847 3909 3994 5065 2385
Size: 462 1062 85 1071 3168
Fragments arranged by size:
3168 1071 1062 462 85
BsmI GAATG_Cn'
Cuts at: 3359 3452 3359
Size: 93 5755
BsmBI CGTCTCn'nnnn_
Cuts at: 2352 2352
Size: 5848
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 2847 2870 2999 3035 3167 3909
Size: 399 595 462 23 129 36 132 742
Cuts at: 3909 3994 5065 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 595 462 399 132 129
85 36 23
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 2211 3737 5467 2211
Size: 1526 1730 2592
Fragments arranged by size:
2592 1730 1526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2858 3203 844
Size: 2014 345 3489
Fragments arranged by size:
3489 2014 345
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 1394
Size: 429 1912 3507
Fragments arranged by size:
3507 1912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4343 4517 65
Size: 1183 3095 174 1396
Fragments arranged by size:
3095 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4492 4679 1700
Size: 110 2682 187 2869
Fragments arranged by size:
2869 2682 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5848
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5848
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 2198 1184
Size: 1014 4834
BstYI r'GATC_y
Cuts at: 1972 2790 2934 3178 3545 3930 3947 5399 5485
Size: 818 144 244 367 385 17 1452 86
Cuts at: 5485 5497 5843 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 818 385 367 346 244 144
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2052 2617 2052
Size: 565 5283
BtgI C'CryG_G
Cuts at: 513 1065 2217 2293 2446 2647 2721 2991 513
Size: 552 1152 76 153 201 74 270 3370
Fragments arranged by size:
3370 1152 552 270 201 153 76 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 2437 3364 4158 4178 2437
Size: 927 794 20 4107
Fragments arranged by size:
4107 927 794 20
ClaI AT'CG_AT
Cuts at: 1554 2568 1554
Size: 1014 4834
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2404 2806
Size: 54 1425 50 275 18 264 310 402
Cuts at: 2806 3295 4184 5813 8
Size: 489 889 1629 43
Fragments arranged by size:
1629 1425 889 489 402 310 275 264
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2528 2954 3778 1353
Size: 1175 426 824 3423
Fragments arranged by size:
3423 1175 824 426
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2744 4435 4809 4955 387
Size: 1064 138 1155 1691 374 146 1280
Fragments arranged by size:
1691 1280 1155 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2552 3911 4508 5295 5563 1239
Size: 393 920 1359 597 787 268 1524
Fragments arranged by size:
1524 1359 920 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3157 3535 3157
Size: 378 5470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 5848
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2458 4995 1678
Size: 780 2537 2531
Fragments arranged by size:
2537 2531 780
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 2228 2260 4489
Size: 32 135 32 560 32 64 32 2229
Cuts at: 4489 4521 4563 4595 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2229 560 135 64 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3192 3438 669
Size: 988 281 1254 246 3079
Fragments arranged by size:
3079 1254 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2883 3039 3084 4962 5146 1305
Size: 1578 156 45 1878 184 2007
Fragments arranged by size:
2007 1878 1578 184 156 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 2808 10
Size: 54 1425 343 264 712 3050
Fragments arranged by size:
3050 1425 712 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2720 3806 4165 4324 518
Size: 648 1050 504 1086 359 159 2042
Fragments arranged by size:
2042 1086 1050 648 504 359 159
MspA1I CmG'CkG
Cuts at: 1606 2605 2987 3058 3264 3941 5089 5334 1606
Size: 999 382 71 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 999 677 382 245 206 71
NcoI C'CATG_G
Cuts at: 513 1065 2721 513
Size: 552 1656 3640
Fragments arranged by size:
3640 1656 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2894 3206 4751 1132
Size: 97 1665 312 1545 2229
Fragments arranged by size:
2229 1665 1545 312 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5848
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2833 1108
Size: 1725 4123
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PstI C_TGCA'G
Cuts at: 830 2778 2958 3200 830
Size: 1948 180 242 3478
Fragments arranged by size:
3478 1948 242 180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2987 3058 2987
Size: 71 5777
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SapI GCTCTTCn'nnn_
Cuts at: 2528 2528
Size: 5848
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 2522 4096 5808 2522
Size: 1574 1712 2562
Fragments arranged by size:
2562 1712 1574
SfcI C'TryA_G
Cuts at: 826 1045 2160 2774 2954 3196 4331 5012 5203
Size: 219 1115 614 180 242 1135 681 191
Cuts at: 5203 826
Size: 1471
Fragments arranged by size:
1471 1135 1115 681 614 242 219 191
180
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 3175 1872
Size: 1303 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 3952 4853 5115 820
Size: 197 926 405 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 405 262 197
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2097 2721 3287 513
Size: 552 1032 624 566 3074
Fragments arranged by size:
3074 1032 624 566 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2036 2677 3875 3892 4045 4230 4675
Size: 1255 456 641 1198 17 153 185 445
Cuts at: 4675 325
Size: 1498
Fragments arranged by size:
1498 1255 1198 641 456 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 4094 5806
Size: 274 80 33 51 1443 543 1574 1712
Cuts at: 5806 96
Size: 138
Fragments arranged by size:
1712 1574 1443 543 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5808 5840 5808
Size: 32 5816
Tth111I GACn'n_nGTC
Cuts at: 1316 2168 1316
Size: 852 4996
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3173 1870
Size: 1303 4545
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI