Restriction map for
pFN21AE4361
(Circular) (Six-base) MAPSORT of: pFN21AE4361.seq Check: 1974 from: 1 to: 5845
pFN21AE4361 5845 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2383 3117 3655 278
Size: 53 83 186 936 847 734 538 2468
Fragments arranged by size:
2468 936 847 734 538 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3102 3117 3188 1937
Size: 1165 15 71 4594
Fragments arranged by size:
4594 1165 71 15
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 3908 5292 5560 1632
Size: 488 1788 1384 268 1917
Fragments arranged by size:
1917 1788 1384 488 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 4744 5721 1203
Size: 3541 977 1327
Fragments arranged by size:
3541 1327 977
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5845
AlwNI CAG_nnn'CTG
Cuts at: 2477 2892 3588 5160 2477
Size: 415 696 1572 3162
Fragments arranged by size:
3162 1572 696 415
ApaI G_GGCC'C
Cuts at: 2471 2471
Size: 5845
ApaLI G'TGCA_C
Cuts at: 3902 5058 3902
Size: 1156 4689
ApoI r'AATT_y
Cuts at: 1493 2057 3154 3380 3532 5641 1493
Size: 564 1097 226 152 2109 1697
Fragments arranged by size:
2109 1697 1097 564 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 2300 3170 1870
Size: 73 357 870 4545
Fragments arranged by size:
4545 870 357 73
AvrII C'CTAG_G
Cuts at: 2777 2777
Size: 5845
BamHI G'GATC_C
Cuts at: 3175 3542 3175
Size: 367 5478
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2528 3166 4621 618
Size: 325 217 627 741 638 1455 1842
Fragments arranged by size:
1842 1455 741 638 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2471 2776 2949 3044 3164 1391
Size: 1080 305 173 95 120 4072
Fragments arranged by size:
4072 1080 305 173 120 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2409 2817 928
Size: 971 510 408 3956
Fragments arranged by size:
3956 971 510 408
BbvCI CC'TCA_GC
Cuts at: 2539 2539
Size: 5845
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4036 4070 1903
Size: 34 2099 34 3678
Fragments arranged by size:
3678 2099 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2237 4456 136
Size: 107 122 71 631 605 565 2219 1525
Fragments arranged by size:
2219 1525 631 605 565 122 107 71
BglII A'GATC_T
Cuts at: 5840 5840
Size: 5845
BlpI GC'TnA_GC
Cuts at: 2943 3262 2943
Size: 319 5526
Bme1580I G_kGCm'C
Cuts at: 1790 2471 2533 3906 5062 1790
Size: 681 62 1373 1156 2573
Fragments arranged by size:
2573 1373 1156 681 62
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2515 3576 4534
Size: 416 53 83 399 24 1098 1061 958
Cuts at: 4534 442
Size: 1753
Fragments arranged by size:
1753 1098 1061 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2412 2412
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4505 1239
Size: 3266 2579
Bpu10I CC'TnA_GC
Cuts at: 2539 2539
Size: 5845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2300 3970 4835 5133 2054
Size: 246 1670 865 298 2766
Fragments arranged by size:
2766 1670 865 298 246
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2237 2592 2616 2676 2712 2718 4508
Size: 617 738 355 24 60 36 6 1790
Cuts at: 4508 882
Size: 2219
Fragments arranged by size:
2219 1790 738 617 355 60 36 24
6
BsaAI yAC'GTr
Cuts at: 493 1204 3636 493
Size: 711 2432 2702
Fragments arranged by size:
2702 2432 711
BsaBI GATnn'nnATC
Cuts at: 2664 2670 2676 5839 2664
Size: 6 6 3163 2670
Fragments arranged by size:
3163 2670 6 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2380 3114 3652 4034
Size: 53 83 186 936 847 734 538 382
Cuts at: 4034 4669 275
Size: 635 1451
Fragments arranged by size:
1451 936 847 734 635 538 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4278 4676 4950 5097 5508 4278
Size: 398 274 147 411 4615
Fragments arranged by size:
4615 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5620
Fragments arranged by size:
5620 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2353 2527 1186
Size: 868 299 174 4504
Fragments arranged by size:
4504 868 299 174
BseYI C'CCAG_C
Cuts at: 5048 5544 5048
Size: 496 5349
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3295 4056 5084 1540
Size: 275 186 1294 761 1028 2301
Fragments arranged by size:
2301 1294 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2949 3906 3991 5062 2949
Size: 957 85 1071 3732
Fragments arranged by size:
3732 1071 957 85
BsmI GAATG_Cn'
Cuts at: 3356 3449 3356
Size: 93 5752
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2471 2533 2776 2949 3044 3164 3906
Size: 399 681 62 243 173 95 120 742
Cuts at: 3906 3991 5062 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 681 399 243 173 120
95 85 62
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 3734 5464 3734
Size: 1730 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2370 2736 3200 844
Size: 1526 366 464 3489
Fragments arranged by size:
3489 1526 464 366
BsrBI CCG'CTC
Cuts at: 1394 1823 2614 2650 2710 3732 1394
Size: 429 791 36 60 1022 3507
Fragments arranged by size:
3507 1022 791 429 60 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 2087 4340 4514 65
Size: 1183 839 2253 174 1396
Fragments arranged by size:
2253 1396 1183 839 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4489 4676 1700
Size: 110 2679 187 2869
Fragments arranged by size:
2869 2679 187 110
BsrGI T'GTAC_A
Cuts at: 96 2971 96
Size: 2875 2970
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5845
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 3175 3542 3927 3944 5396 5482 5494 5840
Size: 1203 367 385 17 1452 86 12 346
Cuts at: 5840 1972
Size: 1977
Fragments arranged by size:
1977 1452 1203 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3066 3066
Size: 5845
BtgI C'CryG_G
Cuts at: 513 1065 2833 2874 513
Size: 552 1768 41 3484
Fragments arranged by size:
3484 1768 552 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5106
Fragments arranged by size:
5106 683 56
BtsI GCAGTG_nn'
Cuts at: 2530 3361 4155 4175 2530
Size: 831 794 20 4200
Fragments arranged by size:
4200 831 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 809
Size: 507 3536 1802
Fragments arranged by size:
3536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3292 4181 5810
Size: 54 1425 50 275 18 1462 889 1629
Cuts at: 5810 8
Size: 43
Fragments arranged by size:
1629 1462 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3292 1537
Size: 275 1480 4090
Fragments arranged by size:
4090 1480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 2451 3775 1353
Size: 678 420 1324 3423
Fragments arranged by size:
3423 1324 678 420
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2947 2947
Size: 5845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 3908 4505 5292 5560 1239
Size: 393 488 1788 597 787 268 1524
Fragments arranged by size:
1788 1524 787 597 488 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2781 2781
Size: 5845
EcoO109I rG'GnC_Cy
Cuts at: 2296 2467 2735 2838 2955 3090 2296
Size: 171 268 103 117 135 5051
Fragments arranged by size:
5051 268 171 135 117 103
EcoRI G'AATT_C
Cuts at: 3154 3532 3154
Size: 378 5467
FspI TGC'GCA
Cuts at: 4351 4351
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 4992 1678
Size: 3314 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4486 4518 4560 4592 1373
Size: 32 135 32 2914 32 42 32 2626
Fragments arranged by size:
2914 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2986 3189 3435 669
Size: 988 281 1048 203 246 3079
Fragments arranged by size:
3079 1048 988 281 246 203
HindIII A'AGCT_T
Cuts at: 748 2497 748
Size: 1749 4096
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2730 4959 5143 1305
Size: 1425 2229 184 2007
Fragments arranged by size:
2229 2007 1425 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4023
Fragments arranged by size:
4023 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3803 4162 4321 518
Size: 648 843 1794 359 159 2042
Fragments arranged by size:
2042 1794 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2206 3261 3938 5086 5331 1606
Size: 600 1055 677 1148 245 2120
Fragments arranged by size:
2120 1148 1055 677 600 245
NcoI C'CATG_G
Cuts at: 513 1065 2874 513
Size: 552 1809 3484
Fragments arranged by size:
3484 1809 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 3203 4748 1132
Size: 97 1974 1545 2229
Fragments arranged by size:
2229 1974 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2471 1348
Size: 1123 4722
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2477 1108
Size: 1369 4476
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2296 2735 2955 3090 2296
Size: 439 220 135 5051
Fragments arranged by size:
5051 439 220 135
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PspOMI G'GGCC_C
Cuts at: 2467 2467
Size: 5845
PstI C_TGCA'G
Cuts at: 830 2052 3197 830
Size: 1222 1145 3478
Fragments arranged by size:
3478 1222 1145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 2206 2206
Size: 5845
SacI G_AGCT'C
Cuts at: 2949 2949
Size: 5845
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SanDI GG'GwC_CC
Cuts at: 2955 2955
Size: 5845
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SfcI C'TryA_G
Cuts at: 826 1045 2048 3103 3193 4328 5009 5200 826
Size: 219 1003 1055 90 1135 681 191 1471
Fragments arranged by size:
1471 1135 1055 1003 681 219 191 90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 2279 3949 4850 5112 820
Size: 197 926 126 210 1670 901 262 1553
Fragments arranged by size:
1670 1553 926 901 262 210 197 126
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5845
StyI C'CwwG_G
Cuts at: 513 1065 2131 2777 2874 3284 513
Size: 552 1066 646 97 410 3074
Fragments arranged by size:
3074 1066 646 552 410 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2616 2736 2742 2836 2899 3872 3889
Size: 1255 1036 120 6 94 63 973 17
Cuts at: 3889 4042 4227 4672 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1036 973 445 185 153 120
94 63 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2971 4091 5803
Size: 274 80 33 51 1443 994 1120 1712
Cuts at: 5803 96
Size: 138
Fragments arranged by size:
1712 1443 1120 994 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5805 5837 5805
Size: 32 5813
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 2057 3974 2057
Size: 1917 3928
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2381 3115 3653 276
Size: 53 83 186 936 847 734 538 2468
Fragments arranged by size:
2468 936 847 734 538 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI