(Circular) (Six-base) MAPSORT of: pFN21AE4360.seq Check: 2107 from: 1 to: 5842 pFN21AE4360 5842 bp With 182 enzymes: SgfI * March 28, 2012 14:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2882 3652 278 Size: 53 83 186 936 1346 770 2468 Fragments arranged by size: 2468 1346 936 770 186 83 53 AccI GT'mk_AC Cuts at: 1937 3185 1937 Size: 1248 4594 Acc65I G'GTAC_C Cuts at: 3163 3163 Size: 5842 AclI AA'CG_TT Cuts at: 1563 3969 4342 1563 Size: 2406 373 3063 Fragments arranged by size: 3063 2406 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 3905 5289 5557 1632 Size: 578 1695 1384 268 1917 Fragments arranged by size: 1917 1695 1384 578 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5645 AflIII A'CryG_T Cuts at: 1203 4741 5718 1203 Size: 3538 977 1327 Fragments arranged by size: 3538 1327 977 AgeI A'CCGG_T Cuts at: 4673 4673 Size: 5842 AhdI GACnn_n'nnGTC Cuts at: 4571 4571 Size: 5842 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2334 2603 1184 Size: 1150 269 4423 Fragments arranged by size: 4423 1150 269 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2685 2717 2685 Size: 32 5810 AlwNI CAG_nnn'CTG Cuts at: 2188 2764 2930 3585 5157 2188 Size: 576 166 655 1572 2873 Fragments arranged by size: 2873 1572 655 576 166 ApaLI G'TGCA_C Cuts at: 3899 5055 3899 Size: 1156 4686 ApoI r'AATT_y Cuts at: 1493 3005 3151 3377 3529 5638 1493 Size: 1512 146 226 152 2109 1697 Fragments arranged by size: 2109 1697 1512 226 152 146 AseI AT'TA_AT Cuts at: 160 1032 4396 160 Size: 872 3364 1606 Fragments arranged by size: 3364 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 AvaI C'yCGr_G Cuts at: 1870 1943 2370 3167 1870 Size: 73 427 797 4545 Fragments arranged by size: 4545 797 427 73 BamHI G'GATC_C Cuts at: 3172 3539 3172 Size: 367 5475 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2489 2528 3163 4618 618 Size: 325 217 627 702 39 635 1455 1842 Fragments arranged by size: 1842 1455 702 635 627 325 217 39 BanII G_rGCy'C Cuts at: 1391 2181 2652 2914 3161 1391 Size: 790 471 262 247 4072 Fragments arranged by size: 4072 790 471 262 247 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2198 928 Size: 971 299 4572 Fragments arranged by size: 4572 971 299 BbvCI CC'TCA_GC Cuts at: 2028 2028 Size: 5842 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3011 3045 4033 4067 1903 Size: 34 1074 34 988 34 3678 Fragments arranged by size: 3678 1074 988 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3734 4944 3734 Size: 1210 4632 BclI T'GATC_A Cuts at: 1548 2341 1548 Size: 793 5049 BfrBI ATG'CAT Cuts at: 2462 2462 Size: 5842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2779 4453 136 Size: 107 122 71 631 605 1107 1674 1525 Fragments arranged by size: 1674 1525 1107 631 605 122 107 71 BglII A'GATC_T Cuts at: 2511 5837 2511 Size: 3326 2516 BlpI GC'TnA_GC Cuts at: 2814 3259 2814 Size: 445 5397 Bme1580I G_kGCm'C Cuts at: 1790 2202 2494 3085 3903 5059 1790 Size: 412 292 591 818 1156 2573 Fragments arranged by size: 2573 1156 818 591 412 292 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5842 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2260 2319 2331 Size: 416 53 83 399 24 843 59 12 Cuts at: 2331 2488 2950 3573 4531 442 Size: 157 462 623 958 1753 Fragments arranged by size: 1753 958 843 623 462 416 399 157 83 59 53 24 12 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4502 1239 Size: 3263 2579 Bpu10I CC'TnA_GC Cuts at: 2028 2799 3033 3057 2028 Size: 771 234 24 4813 Fragments arranged by size: 4813 771 234 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3967 4832 5130 3967 Size: 865 298 4679 Fragments arranged by size: 4679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4505 882 Size: 617 3006 2219 Fragments arranged by size: 3006 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3633 493 Size: 711 2429 2702 Fragments arranged by size: 2702 2429 711 BsaBI GATnn'nnATC Cuts at: 5836 5836 Size: 5842 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 2058 2879 3649 Size: 53 83 186 936 512 13 821 770 Cuts at: 3649 4031 4666 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 821 770 635 512 382 186 83 53 13 BsaWI w'CCGG_w Cuts at: 3000 4275 4673 4947 5094 5505 3000 Size: 1275 398 274 147 411 3337 Fragments arranged by size: 3337 1275 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5617 Fragments arranged by size: 5617 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2388 2495 2624 1186 Size: 1202 107 129 4404 Fragments arranged by size: 4404 1202 129 107 BseYI C'CCAG_C Cuts at: 2181 2302 2485 2601 2930 5045 5541 2181 Size: 121 183 116 329 2115 496 2482 Fragments arranged by size: 2482 2115 496 329 183 121 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2528 2942 1163 Size: 573 792 414 4063 Fragments arranged by size: 4063 792 573 414 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3292 4053 5081 1540 Size: 275 186 1291 761 1028 2301 Fragments arranged by size: 2301 1291 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2718 3903 3988 5059 2718 Size: 1185 85 1071 3501 Fragments arranged by size: 3501 1185 1071 85 BsmI GAATG_Cn' Cuts at: 3353 3446 3353 Size: 93 5749 BsmBI CGTCTCn'nnnn_ Cuts at: 2870 2870 Size: 5842 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2202 2494 2652 2718 2914 3085 Size: 399 391 21 292 158 66 196 171 Cuts at: 3085 3161 3903 3988 5059 1391 Size: 76 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 391 292 196 171 158 85 76 66 21 BspEI T'CCGG_A Cuts at: 3000 5505 3000 Size: 2505 3337 BspHI T'CATG_A Cuts at: 2838 3731 5461 2838 Size: 893 1730 3219 Fragments arranged by size: 3219 1730 893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3197 844 Size: 2353 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2980 3729 1394 Size: 429 1157 749 3507 Fragments arranged by size: 3507 1157 749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4337 4511 65 Size: 1183 3089 174 1396 Fragments arranged by size: 3089 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4486 4673 1700 Size: 110 2676 187 2869 Fragments arranged by size: 2869 2676 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5842 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5842 BssSI C'ACGA_G Cuts at: 3902 4914 3902 Size: 1012 4830 BstAPI GCAn_nnn'nTGC Cuts at: 794 2366 794 Size: 1572 4270 BstBI TT'CG_AA Cuts at: 4646 4646 Size: 5842 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5842 BstYI r'GATC_y Cuts at: 1972 2442 2511 3172 3539 3924 3941 5393 5479 Size: 470 69 661 367 385 17 1452 86 Cuts at: 5479 5491 5837 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 661 470 385 367 346 86 69 17 12 Bsu36I CC'TnA_GG Cuts at: 3090 3090 Size: 5842 BtgI C'CryG_G Cuts at: 513 1065 2895 513 Size: 552 1830 3460 Fragments arranged by size: 3460 1830 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3121 487 Size: 683 56 1895 3208 Fragments arranged by size: 3208 1895 683 56 BtsI GCAGTG_nn' Cuts at: 2020 2560 2762 3358 4152 4172 2020 Size: 540 202 596 794 20 3690 Fragments arranged by size: 3690 794 596 540 202 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5807 DraI TTT'AAA Cuts at: 3146 3146 Size: 5842 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4849 809 Size: 507 3533 1802 Fragments arranged by size: 3533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3289 4178 5807 Size: 54 1425 50 275 18 1459 889 1629 Cuts at: 5807 8 Size: 43 Fragments arranged by size: 1629 1459 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3289 1537 Size: 275 1477 4090 Fragments arranged by size: 4090 1477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3772 1353 Size: 2419 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2069 4429 4803 4949 387 Size: 1064 138 480 2360 374 146 1280 Fragments arranged by size: 2360 1280 1064 480 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 3905 4502 5289 5557 1239 Size: 393 578 1695 597 787 268 1524 Fragments arranged by size: 1695 1524 787 597 578 393 268 EcoO109I rG'GnC_Cy Cuts at: 2333 3046 2333 Size: 713 5129 EcoRI G'AATT_C Cuts at: 3151 3529 3151 Size: 378 5464 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2866 2866 Size: 5842 FspI TGC'GCA Cuts at: 4348 4348 Size: 5842 HaeII r_GCGC'y Cuts at: 1678 4989 1678 Size: 3311 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2600 2632 2816 2848 4483 Size: 32 135 32 1028 32 184 32 1635 Cuts at: 4483 4515 4557 4589 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1635 1028 184 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3186 3432 669 Size: 988 281 1248 246 3079 Fragments arranged by size: 3079 1248 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5842 HpaI GTT'AAC Cuts at: 3432 3432 Size: 5842 KpnI G_GTAC'C Cuts at: 3167 3167 Size: 5842 MfeI C'AATT_G Cuts at: 3441 3441 Size: 5842 MluI A'CGCG_T Cuts at: 5718 5718 Size: 5842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2388 4956 5140 1305 Size: 1083 2568 184 2007 Fragments arranged by size: 2568 2007 1083 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4020 Fragments arranged by size: 4020 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3800 4159 4318 518 Size: 648 2634 359 159 2042 Fragments arranged by size: 2634 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2113 2956 3258 3935 5083 5328 1606 Size: 507 843 302 677 1148 245 2120 Fragments arranged by size: 2120 1148 843 677 507 302 245 NcoI C'CATG_G Cuts at: 513 1065 2895 513 Size: 552 1830 3460 Fragments arranged by size: 3460 1830 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5842 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5842 NotI GC'GGCC_GC Cuts at: 3289 3289 Size: 5842 NsiI A_TGCA'T Cuts at: 2464 2464 Size: 5842 NspI r_CATG'y Cuts at: 1132 1229 2786 3200 4745 1132 Size: 97 1557 414 1545 2229 Fragments arranged by size: 2229 1557 1545 414 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5842 PciI A'CATG_T Cuts at: 4741 4741 Size: 5842 PflMI CCAn_nnn'nTGG Cuts at: 1108 2901 1108 Size: 1793 4049 PfoI T'CCnGG_A Cuts at: 2329 2329 Size: 5842 PmeI GTTT'AAAC Cuts at: 3146 3146 Size: 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2011 2043 3911 3943 5424 5456 2011 Size: 32 1868 32 1481 32 2397 Fragments arranged by size: 2397 1868 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2333 3046 2333 Size: 713 5129 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5842 PsiI TTA'TAA Cuts at: 3412 3412 Size: 5842 PspXI vC'TCGA_Gb Cuts at: 2370 2370 Size: 5842 PstI C_TGCA'G Cuts at: 830 2118 2313 3070 3194 830 Size: 1288 195 757 124 3478 Fragments arranged by size: 3478 1288 757 195 124 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5842 PvuII CAG'CTG Cuts at: 2956 2956 Size: 5842 SalI G'TCGA_C Cuts at: 1936 3184 1936 Size: 1248 4594 SbfI CC_TGCA'GG Cuts at: 3194 3194 Size: 5842 ScaI AGT'ACT Cuts at: 2727 4090 5802 2727 Size: 1363 1712 2767 Fragments arranged by size: 2767 1712 1363 SfcI C'TryA_G Cuts at: 826 1045 2114 2309 3066 3190 4325 5006 5197 Size: 219 1069 195 757 124 1135 681 191 Cuts at: 5197 826 Size: 1471 Fragments arranged by size: 1471 1135 1069 757 681 219 195 191 124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5842 SmaI CCC'GGG Cuts at: 1872 3169 1872 Size: 1297 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2370 3946 4847 5109 820 Size: 197 926 427 1576 901 262 1553 Fragments arranged by size: 1576 1553 926 901 427 262 197 SnaBI TAC'GTA Cuts at: 493 3633 493 Size: 3140 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5842 SphI G_CATG'C Cuts at: 1132 3200 1132 Size: 2068 3774 SspI AAT'ATT Cuts at: 5 52 3766 5 Size: 47 3714 2081 Fragments arranged by size: 3714 2081 47 StuI AGG'CCT Cuts at: 1517 2627 1517 Size: 1110 4732 StyI C'CwwG_G Cuts at: 513 1065 2501 2895 3281 513 Size: 552 1436 394 386 3074 Fragments arranged by size: 3074 1436 552 394 386 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2116 2422 2477 2494 3869 3886 4039 Size: 1255 536 306 55 17 1375 17 153 Cuts at: 4039 4224 4669 325 Size: 185 445 1498 Fragments arranged by size: 1498 1375 1255 536 445 306 185 153 55 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2664 2725 4088 Size: 274 80 33 51 1443 687 61 1363 Cuts at: 4088 5800 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1363 687 274 138 80 61 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5802 5834 5802 Size: 32 5810 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5842 XbaI T'CTAG_A Cuts at: 3178 3178 Size: 5842 XhoI C'TCGA_G Cuts at: 1943 2370 1943 Size: 427 5415 XmaI C'CCGG_G Cuts at: 1870 3167 1870 Size: 1297 4545 XmnI GAAnn'nnTTC Cuts at: 3971 3971 Size: 5842 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2880 3650 276 Size: 53 83 186 936 1346 770 2468 Fragments arranged by size: 2468 1346 936 770 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI