Restriction map for
pFN21AE4358
(Circular) (Six-base) MAPSORT of: pFN21AE4358.seq Check: 3180 from: 1 to: 5830
pFN21AE4358 5830 bp
With 182 enzymes: SgfI *
March 28, 2012 13:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2169 3062 3893 5277 5545 1632
Size: 537 893 831 1384 268 1917
Fragments arranged by size:
1917 1384 893 831 537 268
AfeI AGC'GCT
Cuts at: 2975 2975
Size: 5830
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 2772 4661 2772
Size: 1889 3941
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 2891 1166
Size: 1725 4105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2566 1184
Size: 1382 4448
AlwNI CAG_nnn'CTG
Cuts at: 2408 3573 5145 2408
Size: 1165 1572 3093
Fragments arranged by size:
3093 1572 1165
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 2964 2990 3083 3139 3365 3517 5626 1493
Size: 1471 26 93 56 226 152 2109 1697
Fragments arranged by size:
2109 1697 1471 226 152 93 56 26
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 3155 1870
Size: 73 1212 4545
Fragments arranged by size:
4545 1212 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3151 4606 618
Size: 325 217 627 1364 1455 1842
Fragments arranged by size:
1842 1455 1364 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2050 3149 1391
Size: 659 1099 4072
Fragments arranged by size:
4072 1099 659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 928
Size: 971 102 4757
Fragments arranged by size:
4757 971 102
BbvCI CC'TCA_GC
Cuts at: 2555 2868 2555
Size: 313 5517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4021 4055 1903
Size: 34 2084 34 3678
Fragments arranged by size:
3678 2084 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2904 3102 4441
Size: 107 122 71 631 605 1232 198 1339
Cuts at: 4441 136
Size: 1525
Fragments arranged by size:
1525 1339 1232 631 605 198 122 107
71
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 2175 2847 3891 5047 1790
Size: 385 672 1044 1156 2573
Fragments arranged by size:
2573 1156 1044 672 385
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2102 2162 3561
Size: 416 53 83 399 24 685 60 1399
Cuts at: 3561 4519 442
Size: 958 1753
Fragments arranged by size:
1753 1399 958 685 416 399 83 60
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2961 4490 1239
Size: 1722 1529 2579
Fragments arranged by size:
2579 1722 1529
Bpu10I CC'TnA_GC
Cuts at: 2555 2787 2868 2970 2555
Size: 232 81 102 5415
Fragments arranged by size:
5415 232 102 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2851 3955 4820 5118 2851
Size: 1104 865 298 3563
Fragments arranged by size:
3563 1104 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4493 882
Size: 617 2994 2219
Fragments arranged by size:
2994 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 2933 5824 2933
Size: 2891 2939
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2838 3637 4019 4654
Size: 53 83 186 936 1305 799 382 635
Cuts at: 4654 275
Size: 1451
Fragments arranged by size:
1451 1305 936 799 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2772 4263 4661 4935 5082 5493 2772
Size: 1491 398 274 147 411 3109
Fragments arranged by size:
3109 1491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5605
Fragments arranged by size:
5605 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 2204 1186
Size: 1015 3 4812
Fragments arranged by size:
4812 1015 3
BseYI C'CCAG_C
Cuts at: 5033 5529 5033
Size: 496 5334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2562 1163
Size: 573 826 4431
Fragments arranged by size:
4431 826 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 2550 3891 3976 5047 2304
Size: 246 1341 85 1071 3087
Fragments arranged by size:
3087 1341 1071 246 85
BsmI GAATG_Cn'
Cuts at: 2394 3341 3434 2394
Size: 947 93 4790
Fragments arranged by size:
4790 947 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2175 2304 2550 2847 3149 3891
Size: 399 260 125 129 246 297 302 742
Cuts at: 3891 3976 5047 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 302 297 260 246
129 125 85
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3185 844
Size: 2341 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2772 2793 4474 4661 1700
Size: 110 962 21 1681 187 2869
Fragments arranged by size:
2869 1681 962 187 110 21
BsrGI T'GTAC_A
Cuts at: 96 2256 2931 96
Size: 2160 675 2995
Fragments arranged by size:
2995 2160 675
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 3890 4902 3890
Size: 1012 4818
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5830
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstXI CCAn_nnnn'nTGG
Cuts at: 2432 2758 2893 3062 2432
Size: 326 135 169 5200
Fragments arranged by size:
5200 326 169 135
BstYI r'GATC_y
Cuts at: 1972 2021 3160 3527 3912 3929 5381 5467 5479
Size: 49 1139 367 385 17 1452 86 12
Cuts at: 5479 5825 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1139 385 367 346 86 49
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2232 2705 513
Size: 552 1167 473 3638
Fragments arranged by size:
3638 1167 552 473
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 2550 2771 3346 4140 4160 2550
Size: 221 575 794 20 4220
Fragments arranged by size:
4220 794 575 221 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3277 4166 5795
Size: 54 1425 50 275 18 1447 889 1629
Cuts at: 5795 8
Size: 43
Fragments arranged by size:
1629 1447 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2103 2148 2178 2241 2649 3760 1353
Size: 750 45 30 63 408 1111 3423
Fragments arranged by size:
3423 1111 750 408 63 45 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2169 2961 3062 3893 4490 5277 5545
Size: 393 537 792 101 831 597 787 268
Cuts at: 5545 1239
Size: 1524
Fragments arranged by size:
1524 831 792 787 597 537 393 268
101
EcoO109I rG'GnC_Cy
Cuts at: 2096 2096
Size: 5830
EcoRI G'AATT_C
Cuts at: 2964 3083 3139 3517 2964
Size: 119 56 378 5277
Fragments arranged by size:
5277 378 119 56
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 5830
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 2977 4977 1678
Size: 1299 2000 2531
Fragments arranged by size:
2531 2000 1299
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373
Size: 32 135 32 2899 32 42 32 2626
Fragments arranged by size:
2899 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2258 3096 4944 5128 1305
Size: 953 838 1848 184 2007
Fragments arranged by size:
2007 1848 953 838 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4008
Fragments arranged by size:
4008 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2756 2891 2924 3060 3788 4147 4306
Size: 648 1590 135 33 136 728 359 159
Cuts at: 4306 518
Size: 2042
Fragments arranged by size:
2042 1590 728 648 359 159 136 135
33
MspA1I CmG'CkG
Cuts at: 1606 3246 3923 5071 5316 1606
Size: 1640 677 1148 245 2120
Fragments arranged by size:
2120 1640 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2232 513
Size: 552 1167 4111
Fragments arranged by size:
4111 1167 552
NdeI CA'TA_TG
Cuts at: 387 2293 2999 387
Size: 1906 706 3218
Fragments arranged by size:
3218 1906 706
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2166 3188 4733 1132
Size: 97 937 1022 1545 2229
Fragments arranged by size:
2229 1545 1022 937 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2233 3031 1108
Size: 1125 798 3907
Fragments arranged by size:
3907 1125 798
PfoI T'CCnGG_A
Cuts at: 2983 2983
Size: 5830
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2096 2096
Size: 5830
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PstI C_TGCA'G
Cuts at: 830 3182 830
Size: 2352 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SanDI GG'GwC_CC
Cuts at: 2096 2096
Size: 5830
SapI GCTCTTCn'nnn_
Cuts at: 2148 2649 2148
Size: 501 5329
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2504 3178 4313 4994 5185 826
Size: 219 1459 674 1135 681 191 1471
Fragments arranged by size:
1471 1459 1135 681 674 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2830 3934 4835 5097 820
Size: 197 926 887 1104 901 262 1553
Fragments arranged by size:
1553 1104 926 901 887 262 197
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5830
StyI C'CwwG_G
Cuts at: 513 1065 2232 3269 513
Size: 552 1167 1037 3074
Fragments arranged by size:
3074 1167 1037 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2155 2528 3857 3874 4027 4212 4657
Size: 1255 575 373 1329 17 153 185 445
Cuts at: 4657 325
Size: 1498
Fragments arranged by size:
1498 1329 1255 575 445 373 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2256 2487 2931
Size: 274 80 33 51 1443 279 231 444
Cuts at: 2931 3110 4076 5788 96
Size: 179 966 1712 138
Fragments arranged by size:
1712 1443 966 444 279 274 231 179
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2013 2045 5790 5822 2013
Size: 32 3745 32 2021
Fragments arranged by size:
3745 2021 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2239 2239
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SexAI SfiI SfoI SrfI SwaI