Restriction map for
pFN21AE4352
(Circular) (Six-base) MAPSORT of: pFN21AE4352.seq Check: 8369 from: 1 to: 5812
pFN21AE4352 5812 bp
With 182 enzymes: SgfI *
March 28, 2012 13:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3155 1937
Size: 1218 4594
Acc65I G'GTAC_C
Cuts at: 3133 3133
Size: 5812
AclI AA'CG_TT
Cuts at: 1563 3939 4312 1563
Size: 2376 373 3063
Fragments arranged by size:
3063 2376 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3875 5259 5527 1632
Size: 2243 1384 268 1917
Fragments arranged by size:
2243 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5615
AflIII A'CryG_T
Cuts at: 1203 4711 5688 1203
Size: 3508 977 1327
Fragments arranged by size:
3508 1327 977
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 4541 4541
Size: 5812
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2220 2889 1184
Size: 1036 669 4107
Fragments arranged by size:
4107 1036 669
AlwNI CAG_nnn'CTG
Cuts at: 3555 5127 3555
Size: 1572 4240
ApaI G_GGCC'C
Cuts at: 2677 2677
Size: 5812
ApaLI G'TGCA_C
Cuts at: 2827 3869 5025 2827
Size: 1042 1156 3614
Fragments arranged by size:
3614 1156 1042
ApoI r'AATT_y
Cuts at: 1493 3121 3347 3499 5608 1493
Size: 1628 226 152 2109 1697
Fragments arranged by size:
2109 1697 1628 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4366 160
Size: 872 3334 1606
Fragments arranged by size:
3334 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 2761 3137 1870
Size: 73 818 376 4545
Fragments arranged by size:
4545 818 376 73
BamHI G'GATC_C
Cuts at: 3142 3509 3142
Size: 367 5445
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2153 2434 2515 2957
Size: 325 217 627 242 124 281 81 442
Cuts at: 2957 3133 4588 618
Size: 176 1455 1842
Fragments arranged by size:
1842 1455 627 442 325 281 242 217
176 124 81
BanII G_rGCy'C
Cuts at: 1391 2478 2677 3131 1391
Size: 1087 199 454 4072
Fragments arranged by size:
4072 1087 454 199
BbeI G_GCGC'C
Cuts at: 2033 2438 2033
Size: 405 5407
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 2421 3034 928
Size: 971 354 168 613 3706
Fragments arranged by size:
3706 971 613 354 168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4003 4037 1903
Size: 34 2066 34 3678
Fragments arranged by size:
3678 2066 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 2838 1548
Size: 1290 4522
BfrBI ATG'CAT
Cuts at: 3094 3094
Size: 5812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4423 136
Size: 107 122 71 631 605 2751 1525
Fragments arranged by size:
2751 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5807 5807
Size: 5812
BlpI GC'TnA_GC
Cuts at: 3229 3229
Size: 5812
Bme1580I G_kGCm'C
Cuts at: 1790 2520 2677 2831 2962 3873 5029 1790
Size: 730 157 154 131 911 1156 2573
Fragments arranged by size:
2573 1156 911 730 157 154 131
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2680 2841 3543
Size: 416 53 83 399 24 1263 161 702
Cuts at: 3543 4501 442
Size: 958 1753
Fragments arranged by size:
1753 1263 958 702 416 399 161 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5812
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 4472 1239
Size: 923 2310 2579
Fragments arranged by size:
2579 2310 923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4802 5100 3937
Size: 865 298 4649
Fragments arranged by size:
4649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2339 4475 882
Size: 617 840 2136 2219
Fragments arranged by size:
2219 2136 840 617
BsaAI yAC'GTr
Cuts at: 493 1204 3603 493
Size: 711 2399 2702
Fragments arranged by size:
2702 2399 711
BsaBI GATnn'nnATC
Cuts at: 2314 5806 2314
Size: 3492 2320
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 2435 2609 3030
Size: 53 83 186 936 497 405 174 421
Cuts at: 3030 3619 4001 4636 275
Size: 589 382 635 1451
Fragments arranged by size:
1451 936 635 589 497 421 405 382
186 174 83 53
BsaWI w'CCGG_w
Cuts at: 4245 4643 4917 5064 5475 4245
Size: 398 274 147 411 4582
Fragments arranged by size:
4582 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5587
Fragments arranged by size:
5587 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5812
BseYI C'CCAG_C
Cuts at: 2632 2725 5015 5511 2632
Size: 93 2290 496 2933
Fragments arranged by size:
2933 2290 496 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2278 2384 1163
Size: 573 542 106 4591
Fragments arranged by size:
4591 573 542 106
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3262 4023 5051 1540
Size: 275 186 1261 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2478 2831 3873 3958 5029 2478
Size: 353 1042 85 1071 3261
Fragments arranged by size:
3261 1071 1042 353 85
BsmI GAATG_Cn'
Cuts at: 3323 3416 3323
Size: 93 5719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2478 2520 2677 2831 2962 3131 3873
Size: 399 688 42 157 154 131 169 742
Cuts at: 3873 3958 5029 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 688 399 169 157 154
131 85 42
BspEI T'CCGG_A
Cuts at: 5475 5475
Size: 5812
BspHI T'CATG_A
Cuts at: 3701 5431 3701
Size: 1730 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 3167 844
Size: 1673 650 3489
Fragments arranged by size:
3489 1673 650
BsrBI CCG'CTC
Cuts at: 1394 1823 3699 1394
Size: 429 1876 3507
Fragments arranged by size:
3507 1876 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2871 4307 4481 65
Size: 1183 1623 1436 174 1396
Fragments arranged by size:
1623 1436 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 4456 4643 1700
Size: 110 349 2297 187 2869
Fragments arranged by size:
2869 2297 349 187 110
BsrGI T'GTAC_A
Cuts at: 96 2191 96
Size: 2095 3717
BssHII G'CGCG_C
Cuts at: 1925 2185 1925
Size: 260 5552
BssSI C'ACGA_G
Cuts at: 3872 4884 3872
Size: 1012 4800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5812
BstBI TT'CG_AA
Cuts at: 4616 4616
Size: 5812
BstEII G'GTnAC_C
Cuts at: 1184 2389 1184
Size: 1205 4607
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2446 2305
Size: 141 5671
BstYI r'GATC_y
Cuts at: 1972 2991 3142 3509 3894 3911 5363 5449 5461
Size: 1019 151 367 385 17 1452 86 12
Cuts at: 5461 5807 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1019 385 367 346 151 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2462 2792 2462
Size: 330 5482
BtgI C'CryG_G
Cuts at: 513 1065 2004 2116 513
Size: 552 939 112 4209
Fragments arranged by size:
4209 939 552 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5073
Fragments arranged by size:
5073 683 56
BtsI GCAGTG_nn'
Cuts at: 2135 3328 4122 4142 2135
Size: 1193 794 20 3805
Fragments arranged by size:
3805 1193 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5777
DraI TTT'AAA
Cuts at: 3116 3116
Size: 5812
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2975 4819 809
Size: 507 1659 1844 1802
Fragments arranged by size:
1844 1802 1659 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 3259 4148
Size: 54 1425 50 275 18 216 1213 889
Cuts at: 4148 5777 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1213 889 275 216 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3259 1537
Size: 275 1447 4090
Fragments arranged by size:
4090 1447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3742 1353
Size: 2389 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4399 4773 4919 387
Size: 1064 138 2810 374 146 1280
Fragments arranged by size:
2810 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2476 2476
Size: 5812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 3875 4472 5259 5527 1239
Size: 393 530 1713 597 787 268 1524
Fragments arranged by size:
1713 1524 787 597 530 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2788 2788
Size: 5812
EcoRI G'AATT_C
Cuts at: 3121 3499 3121
Size: 378 5434
FspI TGC'GCA
Cuts at: 4318 4318
Size: 5812
HaeII r_GCGC'y
Cuts at: 1678 2033 2045 2438 4959 1678
Size: 355 12 393 2521 2531
Fragments arranged by size:
2531 2521 393 355 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4453 4485 4527 4559 1373
Size: 32 135 32 2881 32 42 32 2626
Fragments arranged by size:
2881 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3156 3402 669
Size: 988 281 1218 246 3079
Fragments arranged by size:
3079 1218 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5812
HpaI GTT'AAC
Cuts at: 3402 3402
Size: 5812
KasI G'GCGC_C
Cuts at: 2029 2434 2029
Size: 405 5407
KpnI G_GTAC'C
Cuts at: 3137 3137
Size: 5812
MfeI C'AATT_G
Cuts at: 3411 3411
Size: 5812
MluI A'CGCG_T
Cuts at: 5688 5688
Size: 5812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2343 4926 5110 1305
Size: 1038 2583 184 2007
Fragments arranged by size:
2583 2007 1038 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3990
Fragments arranged by size:
3990 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2693 2834 3770 4129 4288 518
Size: 648 1527 141 936 359 159 2042
Fragments arranged by size:
2042 1527 936 648 359 159 141
MspA1I CmG'CkG
Cuts at: 1606 2036 2118 2292 2399 3228 3905 5053 5298
Size: 430 82 174 107 829 677 1148 245
Cuts at: 5298 1606
Size: 2120
Fragments arranged by size:
2120 1148 829 677 430 245 174 107
82
NarI GG'CG_CC
Cuts at: 2030 2435 2030
Size: 405 5407
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4321
Fragments arranged by size:
4321 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5812
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5812
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NsiI A_TGCA'T
Cuts at: 3096 3096
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 3170 4715 1132
Size: 97 1941 1545 2229
Fragments arranged by size:
2229 1941 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5812
PciI A'CATG_T
Cuts at: 4711 4711
Size: 5812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3079 1108
Size: 1971 3841
PfoI T'CCnGG_A
Cuts at: 2478 2772 2478
Size: 294 5518
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 5394 5426 3881
Size: 32 1481 32 4267
Fragments arranged by size:
4267 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2788 2788
Size: 5812
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 3382 3382
Size: 5812
PspOMI G'GGCC_C
Cuts at: 2673 2673
Size: 5812
PstI C_TGCA'G
Cuts at: 830 3164 830
Size: 2334 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
PvuII CAG'CTG
Cuts at: 2292 2399 2292
Size: 107 5705
SacI G_AGCT'C
Cuts at: 2478 2478
Size: 5812
SacII CC_GC'GG
Cuts at: 2119 2119
Size: 5812
SalI G'TCGA_C
Cuts at: 1936 3154 1936
Size: 1218 4594
SbfI CC_TGCA'GG
Cuts at: 3164 3164
Size: 5812
ScaI AGT'ACT
Cuts at: 4060 5772 4060
Size: 1712 4100
SfcI C'TryA_G
Cuts at: 826 1045 3160 4295 4976 5167 826
Size: 219 2115 1135 681 191 1471
Fragments arranged by size:
2115 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2031 2436 2031
Size: 405 5407
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 2763 3139 1872
Size: 891 376 4545
Fragments arranged by size:
4545 891 376
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3916 4817 5079 820
Size: 197 926 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3603 493
Size: 3110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 3170 1132
Size: 2038 3774
SspI AAT'ATT
Cuts at: 5 52 3736 5
Size: 47 3684 2081
Fragments arranged by size:
3684 2081 47
StuI AGG'CCT
Cuts at: 1517 2147 1517
Size: 630 5182
StyI C'CwwG_G
Cuts at: 513 1065 2004 3251 513
Size: 552 939 1247 3074
Fragments arranged by size:
3074 1247 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 3839 3856 4009 4194 4639 325
Size: 1255 525 1734 17 153 185 445 1498
Fragments arranged by size:
1734 1498 1255 525 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2191 4058 5770
Size: 274 80 33 51 1443 214 1867 1712
Cuts at: 5770 96
Size: 138
Fragments arranged by size:
1867 1712 1443 274 214 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5772 5804 5772
Size: 32 5780
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5812
XbaI T'CTAG_A
Cuts at: 3148 3148
Size: 5812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2939 2939
Size: 5812
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5812
XmaI C'CCGG_G
Cuts at: 1870 2761 3137 1870
Size: 891 376 4545
Fragments arranged by size:
4545 891 376
XmnI GAAnn'nnTTC
Cuts at: 3941 3941
Size: 5812
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SrfI SwaI