Restriction map for
pFN21AE4350
(Circular) (Six-base) MAPSORT of: pFN21AE4350.seq Check: 9411 from: 1 to: 5800
pFN21AE4350 5800 bp
With 182 enzymes: SgfI *
March 28, 2012 13:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2276 2320 2395 2725 3863 5247 5515 1632
Size: 644 44 75 330 1138 1384 268 1917
Fragments arranged by size:
1917 1384 1138 644 330 268 75 44
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 2311 1166
Size: 1145 4655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2921 1184
Size: 1737 4063
AlwNI CAG_nnn'CTG
Cuts at: 2530 2966 3543 5115 2530
Size: 436 577 1572 3215
Fragments arranged by size:
3215 1572 577 436
ApaI G_GGCC'C
Cuts at: 2016 2016
Size: 5800
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 3109 3335 3487 5596 1493
Size: 1616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2141 2451 2509 3125 1870
Size: 73 89 109 310 58 616 4545
Fragments arranged by size:
4545 616 310 109 89 73 58
BamHI G'GATC_C
Cuts at: 2266 2277 3130 3497 2266
Size: 11 853 367 4569
Fragments arranged by size:
4569 853 367 11
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 2330 3121 4576 618
Size: 325 217 627 518 25 791 1455 1842
Fragments arranged by size:
1842 1455 791 627 518 325 217 25
BanII G_rGCy'C
Cuts at: 1391 2016 2025 2375 2663 2720 2973 2991 3009
Size: 625 9 350 288 57 253 18 18
Cuts at: 3009 3033 3119 1391
Size: 24 86 4072
Fragments arranged by size:
4072 625 350 288 253 86 57 24
18 18 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2943 928
Size: 971 1044 3785
Fragments arranged by size:
3785 1044 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2753 4411 136
Size: 107 122 71 631 605 1081 1658 1525
Fragments arranged by size:
1658 1525 1081 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2391 5795 2391
Size: 3404 2396
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2292 2308 2516 3861 5017 1790
Size: 226 276 16 208 1345 1156 2573
Fragments arranged by size:
2573 1345 1156 276 226 208 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2723 3531 4489
Size: 416 53 83 399 24 1306 808 958
Cuts at: 4489 442
Size: 1753
Fragments arranged by size:
1753 1306 958 808 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3028 3041 4460 1239
Size: 1789 13 1419 2579
Fragments arranged by size:
2579 1789 1419 13
Bpu10I CC'TnA_GC
Cuts at: 2967 2979 2985 2967
Size: 12 6 5782
Fragments arranged by size:
5782 12 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3023 3925 4790 5088 3023
Size: 902 865 298 3735
Fragments arranged by size:
3735 902 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2312 2470 3071 4463 882
Size: 617 813 158 601 1392 2219
Fragments arranged by size:
2219 1392 813 617 601 158
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 2237 5794 2237
Size: 3557 2243
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 3607 3989 4624
Size: 53 83 186 936 579 1495 382 635
Cuts at: 4624 275
Size: 1451
Fragments arranged by size:
1495 1451 936 635 579 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2100 2823 4233 4631 4905 5052 5463 2100
Size: 723 1410 398 274 147 411 2437
Fragments arranged by size:
2437 1410 723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5575
Fragments arranged by size:
5575 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2651 2960 1186
Size: 1465 309 4026
Fragments arranged by size:
4026 1465 309
BseYI C'CCAG_C
Cuts at: 2698 2789 2972 2996 3050 3056 5003 5499 2698
Size: 91 183 24 54 6 1947 496 2999
Fragments arranged by size:
2999 1947 496 183 91 54 24 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2576 1163
Size: 573 840 4387
Fragments arranged by size:
4387 840 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2887 3250 4011 5039 1540
Size: 275 186 886 363 761 1028 2301
Fragments arranged by size:
2301 1028 886 761 363 275 186
BsiHKAI G_wGCw'C
Cuts at: 2375 3861 3946 5017 2375
Size: 1486 85 1071 3158
Fragments arranged by size:
3158 1486 1071 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2025 2292 2308 2375 2516 2663
Size: 399 226 9 267 16 67 141 147
Cuts at: 2663 2720 2973 2991 3009 3033 3119 3861 3946
Size: 57 253 18 18 24 86 742 85
Cuts at: 3946 5017 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 267 253 226 147
141 86 85 67 57 24 18 18
16 9
BspEI T'CCGG_A
Cuts at: 2823 5463 2823
Size: 2640 3160
BspHI T'CATG_A
Cuts at: 2367 3689 5419 2367
Size: 1322 1730 2748
Fragments arranged by size:
2748 1730 1322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3155 844
Size: 2311 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2283 2302 3687 1394
Size: 429 460 19 1385 3507
Fragments arranged by size:
3507 1385 460 429 19
BsrDI GCAATG_nn'
Cuts at: 65 1248 4295 4469 65
Size: 1183 3047 174 1396
Fragments arranged by size:
3047 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 2797 2797
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2028 2266 2277 2391 2962 3130 3497 3882
Size: 56 238 11 114 571 168 367 385
Cuts at: 3882 3899 5351 5437 5449 5795 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 571 385 367 346 238 168
114 86 56 17 12 11
Bsu36I CC'TnA_GG
Cuts at: 2531 2531
Size: 5800
BtgI C'CryG_G
Cuts at: 513 1065 2004 2664 513
Size: 552 939 660 3649
Fragments arranged by size:
3649 939 660 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 3316 4110 4130 3316
Size: 794 20 4986
Fragments arranged by size:
4986 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2462 4807 809
Size: 507 1146 2345 1802
Fragments arranged by size:
2345 1802 1146 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3064 3247 4136
Size: 54 1425 50 275 18 1234 183 889
Cuts at: 4136 5765 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1234 889 275 183 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3730 1353
Size: 2377 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 4387 4761 4907 387
Size: 1064 138 585 2213 374 146 1280
Fragments arranged by size:
2213 1280 1064 585 374 146 138
EcoICRI GAG'CTC
Cuts at: 2373 2373
Size: 5800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2320 2395 2725 3028 3041 3863
Size: 393 644 44 75 330 303 13 822
Cuts at: 3863 4460 5247 5515 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 822 787 644 597 393 330 303
268 75 44 13
EcoO109I rG'GnC_Cy
Cuts at: 2506 2814 2506
Size: 308 5492
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 2872 2425
Size: 447 5353
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 2748 4947 1678
Size: 1070 2199 2531
Fragments arranged by size:
2531 2199 1070
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2362 2394 4441
Size: 32 135 32 476 32 282 32 2047
Cuts at: 4441 4473 4515 4547 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2047 476 282 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2793 4914 5098 1305
Size: 1488 2121 184 2007
Fragments arranged by size:
2121 2007 1488 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2311 3758 4117 4276 518
Size: 648 1145 1447 359 159 2042
Fragments arranged by size:
2042 1447 1145 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2698 3216 3893 5041 5286 1606
Size: 1092 518 677 1148 245 2120
Fragments arranged by size:
2120 1148 1092 677 518 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2928 1348
Size: 1580 4220
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5800
PfoI T'CCnGG_A
Cuts at: 2273 2343 2273
Size: 70 5730
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2506 2506
Size: 5800
PshAI GACnn'nnGTC
Cuts at: 1613 2805 1613
Size: 1192 4608
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PspOMI G'GGCC_C
Cuts at: 2012 2012
Size: 5800
PstI C_TGCA'G
Cuts at: 830 2646 3152 830
Size: 1816 506 3478
Fragments arranged by size:
3478 1816 506
PvuI CG_AT'CG
Cuts at: 2001 2887 2001
Size: 886 4914
SacI G_AGCT'C
Cuts at: 2375 2375
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 2642 3148 4283 4964 5155 826
Size: 219 1597 506 1135 681 191 1471
Fragments arranged by size:
1597 1471 1135 681 506 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 2034 2453 2511 3127 1872
Size: 162 419 58 616 4545
Fragments arranged by size:
4545 616 419 162 58
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2141 3002 3904 4805 5067 820
Size: 197 926 198 861 902 901 262 1553
Fragments arranged by size:
1553 926 902 901 861 262 198 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2405 3071 1517
Size: 888 666 4246
Fragments arranged by size:
4246 888 666
StyI C'CwwG_G
Cuts at: 513 1065 2004 2400 2418 2424 3239 513
Size: 552 939 396 18 6 815 3074
Fragments arranged by size:
3074 939 815 552 396 18 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2183 2318 3102 3827 3844 3997 4182
Size: 1255 603 135 784 725 17 153 185
Cuts at: 4182 4627 325
Size: 445 1498
Fragments arranged by size:
1498 1255 784 725 603 445 185 153
135 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2133 2352 4046
Size: 274 80 33 51 1443 156 219 1694
Cuts at: 4046 5758 96
Size: 1712 138
Fragments arranged by size:
1712 1694 1443 274 219 156 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5760 5792 5760
Size: 32 5768
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3016 3016
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 2141 1943
Size: 198 5602
XmaI C'CCGG_G
Cuts at: 1870 2032 2451 2509 3125 1870
Size: 162 419 58 616 4545
Fragments arranged by size:
4545 616 419 162 58
XmnI GAAnn'nnTTC
Cuts at: 2855 3929 2855
Size: 1074 4726
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI