Restriction map for
pFN21AE4346
(Circular) (Six-base) MAPSORT of: pFN21AE4346.seq Check: 5360 from: 1 to: 5788
pFN21AE4346 5788 bp
With 182 enzymes: SgfI *
March 28, 2012 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2560 2842 2560
Size: 282 5506
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2634 3598 278
Size: 53 83 186 936 1098 964 2468
Fragments arranged by size:
2468 1098 964 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2712 3131 1937
Size: 775 419 4594
Fragments arranged by size:
4594 775 419
Acc65I G'GTAC_C
Cuts at: 2910 3109 2910
Size: 199 5589
AclI AA'CG_TT
Cuts at: 1563 2795 3915 4288 1563
Size: 1232 1120 373 3063
Fragments arranged by size:
3063 1232 1120 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3851 5235 5503 1632
Size: 2219 1384 268 1917
Fragments arranged by size:
2219 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 2013 4687 5664 1203
Size: 810 2674 977 1327
Fragments arranged by size:
2674 1327 977 810
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 2408 1166
Size: 1242 4546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2900 1184
Size: 1716 4072
AlwNI CAG_nnn'CTG
Cuts at: 3531 5103 3531
Size: 1572 4216
ApaI G_GGCC'C
Cuts at: 2513 2513
Size: 5788
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 3097 3323 3475 5584 1493
Size: 1604 226 152 2109 1697
Fragments arranged by size:
2109 1697 1604 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2177 2242 3113 1870
Size: 73 234 65 871 4545
Fragments arranged by size:
4545 871 234 73 65
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2144 2910 2978 3109
Size: 325 217 627 351 6 766 68 131
Cuts at: 3109 4564 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 766 627 351 325 217 131
68 6
BanII G_rGCy'C
Cuts at: 1391 2513 2594 3107 1391
Size: 1122 81 513 4072
Fragments arranged by size:
4072 1122 513 81
BbeI G_GCGC'C
Cuts at: 2142 2142
Size: 5788
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4817
BbvCI CC'TCA_GC
Cuts at: 2366 2366
Size: 5788
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2953 2987 3979 4013 1903
Size: 34 1016 34 992 34 3678
Fragments arranged by size:
3678 1016 992 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2041 2204 2808
Size: 107 122 71 631 605 369 163 604
Cuts at: 2808 3029 4399 136
Size: 221 1370 1525
Fragments arranged by size:
1525 1370 631 605 604 369 221 163
122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 2249 2513 2741 3849 5005 1790
Size: 459 264 228 1108 1156 2573
Fragments arranged by size:
2573 1156 1108 459 264 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 4477 442
Size: 416 53 83 399 24 2102 958 1753
Fragments arranged by size:
2102 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2654 2748 3008 4448 1239
Size: 1415 94 260 1440 2579
Fragments arranged by size:
2579 1440 1415 260 94
Bpu10I CC'TnA_GC
Cuts at: 2366 2490 2366
Size: 124 5664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3913 4778 5076 3913
Size: 865 298 4625
Fragments arranged by size:
4625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2522 4451 882
Size: 617 1023 1929 2219
Fragments arranged by size:
2219 1929 1023 617
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 2534 5782 2534
Size: 3248 2540
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2139 2631 3595 3977
Size: 53 83 186 936 606 492 964 382
Cuts at: 3977 4612 275
Size: 635 1451
Fragments arranged by size:
1451 964 936 635 606 492 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2265 4221 4619 4893 5040 5451 2265
Size: 1956 398 274 147 411 2602
Fragments arranged by size:
2602 1956 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2694 2724 1178
Size: 30 165 30 1291 30 4242
Fragments arranged by size:
4242 1291 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2747 2930 1186
Size: 1084 477 183 4044
Fragments arranged by size:
4044 1084 477 183
BseYI C'CCAG_C
Cuts at: 2122 2506 2544 2756 4991 5487 2122
Size: 384 38 212 2235 496 2423
Fragments arranged by size:
2423 2235 496 384 212 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5215
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3849 3934 5005 3849
Size: 85 1071 4632
Fragments arranged by size:
4632 1071 85
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
BsmBI CGTCTCn'nnnn_
Cuts at: 2905 2905
Size: 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2249 2513 2594 2741 3107 3849 3934
Size: 399 459 264 81 147 366 742 85
Cuts at: 3934 5005 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 459 399 366 264 147
85 81
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2560 2842 2931 3143 844
Size: 1716 282 89 212 3489
Fragments arranged by size:
3489 1716 282 212 89
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4283 4457 65
Size: 1183 3035 174 1396
Fragments arranged by size:
3035 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 2141 2718 4432 4619 1700
Size: 110 252 79 577 1714 187 2869
Fragments arranged by size:
2869 1714 577 252 187 110 79
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 2069 2636 3848 4860 2069
Size: 567 1212 1012 2997
Fragments arranged by size:
2997 1212 1012 567
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5788
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2296 2296
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 3118 3485 3870 3887 5339 5425 5437 5783
Size: 1146 367 385 17 1452 86 12 346
Cuts at: 5783 1972
Size: 1977
Fragments arranged by size:
1977 1452 1146 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2200 2200
Size: 5788
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4297
Fragments arranged by size:
4297 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 3304 4098 4118 3304
Size: 794 20 4974
Fragments arranged by size:
4974 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2548 2897 4795 809
Size: 507 1232 349 1898 1802
Fragments arranged by size:
1898 1802 1232 507 349
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2532 3235 4124
Size: 54 1425 50 275 18 702 703 889
Cuts at: 4124 5753 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 703 702 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2948 3718 1353
Size: 1595 770 3423
Fragments arranged by size:
3423 1595 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 4375 4749 4895 387
Size: 1064 138 490 2296 374 146 1280
Fragments arranged by size:
2296 1280 1064 490 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2654 2748 3008 3851 4448 5235 5503
Size: 393 1022 94 260 843 597 787 268
Cuts at: 5503 1239
Size: 1524
Fragments arranged by size:
1524 1022 843 787 597 393 268 260
94
EcoNI CCTnn'n_nnAGG
Cuts at: 2927 2927
Size: 5788
EcoO109I rG'GnC_Cy
Cuts at: 2220 2510 3030 2220
Size: 290 520 4978
Fragments arranged by size:
4978 520 290
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 2142 2199 4935 1678
Size: 464 57 2736 2531
Fragments arranged by size:
2736 2531 464 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2694 2726 4429 4461 4503
Size: 32 135 32 1122 32 1703 32 42
Cuts at: 4503 4535 1373
Size: 32 2626
Fragments arranged by size:
2626 1703 1122 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2320 2350 3132 3378 669
Size: 988 281 382 30 782 246 3079
Fragments arranged by size:
3079 988 782 382 281 246 30
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KasI G'GCGC_C
Cuts at: 2138 2138
Size: 5788
KpnI G_GTAC'C
Cuts at: 2914 3113 2914
Size: 199 5589
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2409 4902 5086 1305
Size: 1104 2493 184 2007
Fragments arranged by size:
2493 2007 1104 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2534 10
Size: 54 1425 343 702 3264
Fragments arranged by size:
3264 1425 702 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 2408 2744 2882 3746 4105 4264
Size: 648 1128 114 336 138 864 359 159
Cuts at: 4264 518
Size: 2042
Fragments arranged by size:
2042 1128 864 648 359 336 159 138
114
MspA1I CmG'CkG
Cuts at: 1606 2122 2425 2506 2848 2917 3204 3881 5029
Size: 516 303 81 342 69 287 677 1148
Cuts at: 5029 5274 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 516 342 303 287 245
81 69
NaeI GCC'GGC
Cuts at: 2064 2143 2064
Size: 79 5709
NarI GG'CG_CC
Cuts at: 2139 2139
Size: 5788
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4297
Fragments arranged by size:
4297 939 552
NdeI CA'TA_TG
Cuts at: 387 2407 387
Size: 2020 3768
NgoMIV G'CCGG_C
Cuts at: 2062 2141 2062
Size: 79 5709
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 2815 3146 4691 1132
Size: 97 1586 331 1545 2229
Fragments arranged by size:
2229 1586 1545 331 97
PasI CC'CwG_GG
Cuts at: 1348 2617 2695 1348
Size: 1269 78 4441
Fragments arranged by size:
4441 1269 78
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2178 3857 3889 5370 5402 2146
Size: 32 1679 32 1481 32 2532
Fragments arranged by size:
2532 1679 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2509 2509
Size: 5788
PstI C_TGCA'G
Cuts at: 830 2853 3140 830
Size: 2023 287 3478
Fragments arranged by size:
3478 2023 287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2425 2506 2848 2917 2425
Size: 81 342 69 5296
Fragments arranged by size:
5296 342 81 69
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 2849 3136 4271 4952 5143 826
Size: 219 1804 287 1135 681 191 1471
Fragments arranged by size:
1804 1471 1135 681 287 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3029 3029
Size: 5788
SfoI GGC'GCC
Cuts at: 2140 2140
Size: 5788
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 2718 1700
Size: 1018 4770
SmaI CCC'GGG
Cuts at: 1872 2179 2244 3115 1872
Size: 307 65 871 4545
Fragments arranged by size:
4545 871 307 65
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3892 4793 5055 820
Size: 197 926 1949 901 262 1553
Fragments arranged by size:
1949 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 2815 3146 1132
Size: 1683 331 3774
Fragments arranged by size:
3774 1683 331
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5788
StyI C'CwwG_G
Cuts at: 513 1065 2004 2928 3025 3227 513
Size: 552 939 924 97 202 3074
Fragments arranged by size:
3074 939 924 552 202 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2447 2687 2846 2966 3815 3832 3985
Size: 1255 867 240 159 120 849 17 153
Cuts at: 3985 4170 4615 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 867 849 445 240 185 159
153 120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2449 4034 5746
Size: 274 80 33 51 1443 472 1585 1712
Cuts at: 5746 96
Size: 138
Fragments arranged by size:
1712 1585 1443 472 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2962 2994 5748 5780 2962
Size: 32 2754 32 2970
Fragments arranged by size:
2970 2754 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2723 2723
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 2177 2242 3113 1870
Size: 307 65 871 4545
Fragments arranged by size:
4545 871 307 65
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2632 3596 276
Size: 53 83 186 936 1098 964 2468
Fragments arranged by size:
2468 1098 964 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SrfI SwaI