Restriction map for
pFN21AE4340
(Circular) (Six-base) MAPSORT of: pFN21AE4340.seq Check: 5979 from: 1 to: 5758
pFN21AE4340 5758 bp
With 182 enzymes: SgfI *
March 28, 2012 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2452 2452
Size: 5758
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 3821 5205 5473 1632
Size: 461 1728 1384 268 1917
Fragments arranged by size:
1917 1728 1384 461 268
AfeI AGC'GCT
Cuts at: 2363 2363
Size: 5758
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 2940 1166
Size: 1774 3984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AlwNI CAG_nnn'CTG
Cuts at: 3501 5073 3501
Size: 1572 4186
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 3067 3293 3445 5554 1493
Size: 1574 226 152 2109 1697
Fragments arranged by size:
2109 1697 1574 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2772 3083 1870
Size: 73 829 311 4545
Fragments arranged by size:
4545 829 311 73
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3079 4534 618
Size: 325 217 627 1292 1455 1842
Fragments arranged by size:
1842 1455 1292 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2379 2454 3077 1391
Size: 988 75 623 4072
Fragments arranged by size:
4072 988 623 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2938 928
Size: 971 1039 3748
Fragments arranged by size:
3748 1039 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2753 2787 2963 2997 3949 3983 1903
Size: 34 816 34 176 34 952 34 3678
Fragments arranged by size:
3678 952 816 176 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2186 4369 136
Size: 107 122 71 631 605 514 2183 1525
Fragments arranged by size:
2183 1525 631 605 514 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 2087 2373 2448 3175 2087
Size: 286 75 727 4670
Fragments arranged by size:
4670 727 286 75
Bme1580I G_kGCm'C
Cuts at: 1790 2385 2633 3819 4975 1790
Size: 595 248 1186 1156 2573
Fragments arranged by size:
2573 1186 1156 595 248
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2612 3489 4447
Size: 416 53 83 399 24 1195 877 958
Cuts at: 4447 442
Size: 1753
Fragments arranged by size:
1753 1195 958 877 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2594 2660 2880 2894 4418 1239
Size: 1355 66 220 14 1524 2579
Fragments arranged by size:
2579 1524 1355 220 66 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2951 3883 4748 5046 2210
Size: 741 932 865 298 2922
Fragments arranged by size:
2922 932 865 741 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4421 882
Size: 617 2922 2219
Fragments arranged by size:
2922 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 2534 5752 2534
Size: 3218 2540
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2348 2378 2963 2993 1178
Size: 30 165 30 945 30 585 30 3943
Fragments arranged by size:
3943 945 585 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5758
BseYI C'CCAG_C
Cuts at: 3015 4961 5457 3015
Size: 1946 496 3316
Fragments arranged by size:
3316 1946 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 2339 2578 2865 1163
Size: 573 399 204 239 287 4056
Fragments arranged by size:
4056 573 399 287 239 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 2159 2379 2454 2895 3819 3904 4975 2088
Size: 71 220 75 441 924 85 1071 2871
Fragments arranged by size:
2871 1071 924 441 220 85 75 71
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
BsmBI CGTCTCn'nnnn_
Cuts at: 2117 2117
Size: 5758
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2159 2379 2385 2454 2633 2895
Size: 399 298 71 220 6 69 179 262
Cuts at: 2895 3077 3819 3904 4975 1391
Size: 182 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 298 262 220 182
179 85 71 69 6
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2452 2837 3113 844
Size: 1608 385 276 3489
Fragments arranged by size:
3489 1608 385 276
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2430 4253 4427 65
Size: 1183 1182 1823 174 1396
Fragments arranged by size:
1823 1396 1183 1182 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2220 4402 4589 1700
Size: 110 410 2182 187 2869
Fragments arranged by size:
2869 2182 410 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 2378 2894 3818 4830 2378
Size: 516 924 1012 3306
Fragments arranged by size:
3306 1012 924 516
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstXI CCAn_nnnn'nTGG
Cuts at: 2470 2470
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753
Size: 1116 367 385 17 1452 86 12 346
Cuts at: 5753 1972
Size: 1977
Fragments arranged by size:
1977 1452 1116 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2751 2751
Size: 5758
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2353 487
Size: 683 56 1127 3892
Fragments arranged by size:
3892 1127 683 56
BtsI GCAGTG_nn'
Cuts at: 2123 3274 4068 4088 2123
Size: 1151 794 20 3793
Fragments arranged by size:
3793 1151 794 20
ClaI AT'CG_AT
Cuts at: 1554 2214 1554
Size: 660 5098
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DraIII CAC_nnn'GTG
Cuts at: 2735 2735
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2805 3205
Size: 54 1425 50 275 18 348 627 400
Cuts at: 3205 4094 5723 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 627 400 348 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2840 3688 1353
Size: 1487 848 3423
Fragments arranged by size:
3423 1487 848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2240 4345 4719 4865 387
Size: 1064 138 651 2105 374 146 1280
Fragments arranged by size:
2105 1280 1064 651 374 146 138
EcoICRI GAG'CTC
Cuts at: 2377 2452 2377
Size: 75 5683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2594 2660 2880 2894 3821 4418
Size: 393 461 501 66 220 14 927 597
Cuts at: 4418 5205 5473 1239
Size: 787 268 1524
Fragments arranged by size:
1524 927 787 597 501 461 393 268
220 66 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2561 2561
Size: 5758
EcoO109I rG'GnC_Cy
Cuts at: 2518 2755 2518
Size: 237 5521
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2851 2935 2851
Size: 84 5674
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 2365 3022 4905 1678
Size: 687 657 1883 2531
Fragments arranged by size:
2531 1883 687 657
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4399 4431 4473 4505 1373
Size: 32 135 32 2827 32 42 32 2626
Fragments arranged by size:
2827 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4872 5056 1305
Size: 3567 184 2007
Fragments arranged by size:
3567 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2180 10
Size: 54 1425 343 348 3588
Fragments arranged by size:
3588 1425 348 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2271 2468 2940 3716 4075 4234 518
Size: 648 1105 197 472 776 359 159 2042
Fragments arranged by size:
2042 1105 776 648 472 359 197 159
MspA1I CmG'CkG
Cuts at: 1606 2767 3174 3851 4999 5244 1606
Size: 1161 407 677 1148 245 2120
Fragments arranged by size:
2120 1161 1148 677 407 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5206
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2735 1108
Size: 1627 4131
PfoI T'CCnGG_A
Cuts at: 2016 2496 2637 2016
Size: 480 141 5137
Fragments arranged by size:
5137 480 141
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2518 2518
Size: 5758
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PstI C_TGCA'G
Cuts at: 830 2154 3110 830
Size: 1324 956 3478
Fragments arranged by size:
3478 1324 956
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 2767 2767
Size: 5758
SacI G_AGCT'C
Cuts at: 2379 2454 2379
Size: 75 5683
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SapI GCTCTTCn'nnn_
Cuts at: 2840 2840
Size: 5758
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5758
SfcI C'TryA_G
Cuts at: 826 1045 2150 3106 4241 4922 5113 826
Size: 219 1105 956 1135 681 191 1471
Fragments arranged by size:
1471 1135 1105 956 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 2966 3862 4763 5025 820
Size: 197 926 282 741 896 901 262 1553
Fragments arranged by size:
1553 926 901 896 741 282 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 2567 1517
Size: 1050 4708
StyI C'CwwG_G
Cuts at: 513 1065 3197 513
Size: 552 2132 3074
Fragments arranged by size:
3074 2132 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2409 2895 3785 3802 3955 4140 4585
Size: 1255 829 486 890 17 153 185 445
Cuts at: 4585 325
Size: 1498
Fragments arranged by size:
1498 1255 890 829 486 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 4004 5716
Size: 274 80 33 51 1443 846 1181 1712
Cuts at: 5716 96
Size: 138
Fragments arranged by size:
1712 1443 1181 846 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2348 2380 2963 2995 5718 5750 2348
Size: 32 583 32 2723 32 2356
Fragments arranged by size:
2723 2356 583 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2203 1316
Size: 887 4871
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2936 2936
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 2930 3887 2930
Size: 957 4801
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI