Restriction map for
pFN21AE4335
(Circular) (Six-base) MAPSORT of: pFN21AE4335.seq Check: 314 from: 1 to: 5740
pFN21AE4335 5740 bp
With 182 enzymes: SgfI *
March 28, 2012 13:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 1937
Size: 1146 4594
Acc65I G'GTAC_C
Cuts at: 2723 3061 2723
Size: 338 5402
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3803 5187 5455 1632
Size: 2171 1384 268 1917
Fragments arranged by size:
2171 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 4571 4571
Size: 5740
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2280 1184
Size: 1096 4644
AlwNI CAG_nnn'CTG
Cuts at: 3483 5055 3483
Size: 1572 4168
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 2120 2827 3049 3275 3427 5536 1493
Size: 627 707 222 226 152 2109 1697
Fragments arranged by size:
2109 1697 707 627 226 222 152
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 2103 2316 3065 1870
Size: 73 160 213 749 4545
Fragments arranged by size:
4545 749 213 160 73
BamHI G'GATC_C
Cuts at: 3070 3437 3070
Size: 367 5373
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2723 3061 4516 618
Size: 325 217 627 936 338 1455 1842
Fragments arranged by size:
1842 1455 936 627 338 325 217
BanII G_rGCy'C
Cuts at: 1391 2996 3059 1391
Size: 1605 63 4072
Fragments arranged by size:
4072 1605 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2421 928
Size: 971 522 4247
Fragments arranged by size:
4247 971 522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3931 3965 1903
Size: 34 1994 34 3678
Fragments arranged by size:
3678 1994 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3632 4842 3632
Size: 1210 4530
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BfrBI ATG'CAT
Cuts at: 2456 2456
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2393 4351 136
Size: 107 122 71 631 605 721 1958 1525
Fragments arranged by size:
1958 1525 721 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5735 5735
Size: 5740
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 3801 4957 1790
Size: 2011 1156 2573
Fragments arranged by size:
2573 2011 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5740
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3471 4429 442
Size: 416 53 83 399 24 2054 958 1753
Fragments arranged by size:
2054 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2399 4400 1239
Size: 1139 21 2001 2579
Fragments arranged by size:
2579 2001 1139 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2290 2503 4403 882
Size: 617 791 213 1900 2219
Fragments arranged by size:
2219 1900 791 617 213
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 5734 5734
Size: 5740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3547 3929 4564 275
Size: 53 83 186 936 2014 382 635 1451
Fragments arranged by size:
2014 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4173 4571 4845 4992 5403 4173
Size: 398 274 147 411 4510
Fragments arranged by size:
4510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2959 2989 1178
Size: 30 165 30 1556 30 3929
Fragments arranged by size:
3929 1556 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2964 2967 2970 2986 1186
Size: 1778 3 3 16 3940
Fragments arranged by size:
3940 1778 16 3 3
BseYI C'CCAG_C
Cuts at: 4943 5439 4943
Size: 496 5244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3801 3886 4957 3801
Size: 85 1071 4584
Fragments arranged by size:
4584 1071 85
BsmI GAATG_Cn'
Cuts at: 3251 3344 3251
Size: 93 5647
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2996 3059 3801 3886 4957 1391
Size: 399 1206 63 742 85 1071 2174
Fragments arranged by size:
2174 1206 1071 742 399 85 63
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2272 3095 844
Size: 1428 823 3489
Fragments arranged by size:
3489 1428 823
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2905 4235 4409 65
Size: 1183 1657 1330 174 1396
Fragments arranged by size:
1657 1396 1330 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4384 4571 1700
Size: 110 2574 187 2869
Fragments arranged by size:
2869 2574 187 110
BsrGI T'GTAC_A
Cuts at: 96 2503 96
Size: 2407 3333
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 3800 4812 3800
Size: 1012 4728
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstYI r'GATC_y
Cuts at: 1972 2776 3070 3437 3822 3839 5291 5377 5389
Size: 804 294 367 385 17 1452 86 12
Cuts at: 5389 5735 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 804 385 367 346 294 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4249
Fragments arranged by size:
4249 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 3256 4050 4070 3256
Size: 794 20 4926
Fragments arranged by size:
4926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 2040 3044 2040
Size: 1004 4736
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2630 4747 809
Size: 507 1314 2117 1802
Fragments arranged by size:
2117 1802 1314 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3187 4076 5705
Size: 54 1425 50 275 18 1357 889 1629
Cuts at: 5705 8
Size: 43
Fragments arranged by size:
1629 1425 1357 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3670 1353
Size: 2317 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2617 4327 4701 4847 387
Size: 1064 138 1028 1710 374 146 1280
Fragments arranged by size:
1710 1280 1064 1028 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 2399 3803 4400 5187 5455 1239
Size: 393 746 21 1404 597 787 268 1524
Fragments arranged by size:
1524 1404 787 746 597 393 268 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2746 2746
Size: 5740
EcoRI G'AATT_C
Cuts at: 2120 3049 3427 2120
Size: 929 378 4433
Fragments arranged by size:
4433 929 378
EcoRV GAT'ATC
Cuts at: 2754 2754
Size: 5740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 2660 2107
Size: 553 5187
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 3017 4887 1678
Size: 1339 1870 2531
Fragments arranged by size:
2531 1870 1339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2831 2863 4381 4413 4455
Size: 32 135 32 1259 32 1518 32 42
Cuts at: 4455 4487 1373
Size: 32 2626
Fragments arranged by size:
2626 1518 1259 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2878 3084 3330 669
Size: 988 281 940 206 246 3079
Fragments arranged by size:
3079 988 940 281 246 206
HindIII A'AGCT_T
Cuts at: 748 2089 748
Size: 1341 4399
HpaI GTT'AAC
Cuts at: 2878 3330 2878
Size: 452 5288
KpnI G_GTAC'C
Cuts at: 2727 3065 2727
Size: 338 5402
MfeI C'AATT_G
Cuts at: 3339 3339
Size: 5740
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 2624 4854 5038 1305
Size: 831 488 2230 184 2007
Fragments arranged by size:
2230 2007 831 488 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3918
Fragments arranged by size:
3918 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2262 2343 3698 4057 4216 518
Size: 648 1096 81 1355 359 159 2042
Fragments arranged by size:
2042 1355 1096 648 359 159 81
MspA1I CmG'CkG
Cuts at: 1606 3156 3833 4981 5226 1606
Size: 1550 677 1148 245 2120
Fragments arranged by size:
2120 1550 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4249
Fragments arranged by size:
4249 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NsiI A_TGCA'T
Cuts at: 2458 2458
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 3098 4643 1132
Size: 97 1869 1545 2229
Fragments arranged by size:
2229 1869 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5740
PfoI T'CCnGG_A
Cuts at: 2094 2124 2094
Size: 30 5710
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5740
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PstI C_TGCA'G
Cuts at: 830 3092 830
Size: 2262 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 3088 4223 4904 5095 826
Size: 219 2043 1135 681 191 1471
Fragments arranged by size:
2043 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3844 4745 5007 820
Size: 197 926 1901 901 262 1553
Fragments arranged by size:
1901 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 3664 5
Size: 47 3612 2081
Fragments arranged by size:
3612 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2004 3179 513
Size: 552 939 1175 3074
Fragments arranged by size:
3074 1175 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3767 3784 3937 4122 4567 325
Size: 1255 2187 17 153 185 445 1498
Fragments arranged by size:
2187 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2170 2503 3986
Size: 274 80 33 51 1443 193 333 1483
Cuts at: 3986 5698 96
Size: 1712 138
Fragments arranged by size:
1712 1483 1443 333 274 193 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2959 2991 5700 5732 2959
Size: 32 2709 32 2967
Fragments arranged by size:
2967 2709 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 2605 3076 2605
Size: 471 5269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2374 2374
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 3869 3869
Size: 5740
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI