Restriction map for
pFN21AE4332
(Circular) (Six-base) MAPSORT of: pFN21AE4332.seq Check: 436 from: 1 to: 5728
pFN21AE4332 5728 bp
With 182 enzymes: SgfI *
March 28, 2012 13:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2742 2742
Size: 5728
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3538 278
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3071 1937
Size: 1134 4594
Acc65I G'GTAC_C
Cuts at: 3049 3049
Size: 5728
AclI AA'CG_TT
Cuts at: 1563 3855 4228 1563
Size: 2292 373 3063
Fragments arranged by size:
3063 2292 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3791 5175 5443 1632
Size: 2159 1384 268 1917
Fragments arranged by size:
2159 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5531
AflIII A'CryG_T
Cuts at: 1203 2472 4627 5604 1203
Size: 1269 2155 977 1327
Fragments arranged by size:
2155 1327 1269 977
AgeI A'CCGG_T
Cuts at: 2717 4559 2717
Size: 1842 3886
AhdI GACnn_n'nnGTC
Cuts at: 4457 4457
Size: 5728
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2301 1184
Size: 1117 4611
AlwNI CAG_nnn'CTG
Cuts at: 3471 5043 3471
Size: 1572 4156
ApaI G_GGCC'C
Cuts at: 2414 2529 2570 2414
Size: 115 41 5572
Fragments arranged by size:
5572 115 41
ApaLI G'TGCA_C
Cuts at: 3785 4941 3785
Size: 1156 4572
ApoI r'AATT_y
Cuts at: 1493 3037 3263 3415 5524 1493
Size: 1544 226 152 2109 1697
Fragments arranged by size:
2109 1697 1544 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4282 160
Size: 872 3250 1606
Fragments arranged by size:
3250 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 2638 3053 1870
Size: 73 695 415 4545
Fragments arranged by size:
4545 695 415 73
BamHI G'GATC_C
Cuts at: 3058 3425 3058
Size: 367 5361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3049 4504 618
Size: 325 217 627 1262 1455 1842
Fragments arranged by size:
1842 1455 1262 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2414 2465 2529 2570 3027 3047 1391
Size: 1023 51 64 41 457 20 4072
Fragments arranged by size:
4072 1023 457 64 51 41 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3919 3953 1903
Size: 34 1982 34 3678
Fragments arranged by size:
3678 1982 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 4830 3620
Size: 1210 4518
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4339 136
Size: 107 122 71 631 605 2667 1525
Fragments arranged by size:
2667 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5723 5723
Size: 5728
BlpI GC'TnA_GC
Cuts at: 3021 3145 3021
Size: 124 5604
Bme1580I G_kGCm'C
Cuts at: 1790 2414 2529 2570 3789 4945 1790
Size: 624 115 41 1219 1156 2573
Fragments arranged by size:
2573 1219 1156 624 115 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2340 3459 4417
Size: 416 53 83 399 24 923 1119 958
Cuts at: 4417 442
Size: 1753
Fragments arranged by size:
1753 1119 958 923 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4388 1239
Size: 3149 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3853 4718 5016 3853
Size: 865 298 4565
Fragments arranged by size:
4565 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2248 4391 882
Size: 617 749 2143 2219
Fragments arranged by size:
2219 2143 749 617
BsaAI yAC'GTr
Cuts at: 493 1204 3519 493
Size: 711 2315 2702
Fragments arranged by size:
2702 2315 711
BsaBI GATnn'nnATC
Cuts at: 5722 5722
Size: 5728
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3535 3917 4552 275
Size: 53 83 186 936 2002 382 635 1451
Fragments arranged by size:
2002 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2717 4161 4559 4833 4980 5391 2717
Size: 1444 398 274 147 411 3054
Fragments arranged by size:
3054 1444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2079 2109 2421 2451 1178
Size: 30 165 30 676 30 312 30 4455
Fragments arranged by size:
4455 676 312 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2771 2774 1186
Size: 1585 3 4140
Fragments arranged by size:
4140 1585 3
BseYI C'CCAG_C
Cuts at: 2403 4931 5427 2403
Size: 2528 496 2704
Fragments arranged by size:
2704 2528 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 1163
Size: 573 432 4723
Fragments arranged by size:
4723 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 3939 4967 1540
Size: 275 186 1177 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 2465 3789 3874 4945 2232
Size: 233 1324 85 1071 3015
Fragments arranged by size:
3015 1324 1071 233 85
BsmI GAATG_Cn'
Cuts at: 3239 3332 3239
Size: 93 5635
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2414 2465 2529 2570 3027 3047
Size: 399 442 182 51 64 41 457 20
Cuts at: 3047 3789 3874 4945 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 442 399 182 85
64 51 41 20
BspEI T'CCGG_A
Cuts at: 5391 5391
Size: 5728
BspHI T'CATG_A
Cuts at: 3617 5347 3617
Size: 1730 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2742 3083 844
Size: 1898 341 3489
Fragments arranged by size:
3489 1898 341
BsrBI CCG'CTC
Cuts at: 1394 1823 3615 1394
Size: 429 1792 3507
Fragments arranged by size:
3507 1792 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2153 4223 4397 65
Size: 1183 905 2070 174 1396
Fragments arranged by size:
2070 1396 1183 905 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2717 4372 4559 1700
Size: 110 907 1655 187 2869
Fragments arranged by size:
2869 1655 907 187 110
BsrGI T'GTAC_A
Cuts at: 96 3000 96
Size: 2904 2824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5728
BssSI C'ACGA_G
Cuts at: 3788 4800 3788
Size: 1012 4716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5728
BstBI TT'CG_AA
Cuts at: 4532 4532
Size: 5728
BstEII G'GTnAC_C
Cuts at: 1184 2720 1184
Size: 1536 4192
BstXI CCAn_nnnn'nTGG
Cuts at: 2422 2628 2422
Size: 206 5522
BstYI r'GATC_y
Cuts at: 1972 3058 3425 3810 3827 5279 5365 5377 5723
Size: 1086 367 385 17 1452 86 12 346
Cuts at: 5723 1972
Size: 1977
Fragments arranged by size:
1977 1452 1086 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2898 2898
Size: 5728
BtgI C'CryG_G
Cuts at: 513 1065 2415 2578 513
Size: 552 1350 163 3663
Fragments arranged by size:
3663 1350 552 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2786 487
Size: 683 56 1560 3429
Fragments arranged by size:
3429 1560 683 56
BtsI GCAGTG_nn'
Cuts at: 2648 2926 3244 4038 4058 2648
Size: 278 318 794 20 4318
Fragments arranged by size:
4318 794 318 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5693
DraI TTT'AAA
Cuts at: 3032 3032
Size: 5728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2063 3175 4064
Size: 54 1425 50 275 18 233 1112 889
Cuts at: 4064 5693 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1112 889 275 233 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3175 1537
Size: 275 1363 4090
Fragments arranged by size:
4090 1363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2124 2777 2792 3658 1353
Size: 771 653 15 866 3423
Fragments arranged by size:
3423 866 771 653 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4315 4689 4835 387
Size: 1064 138 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2463 2463
Size: 5728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3791 4388 5175 5443 1239
Size: 393 2159 597 787 268 1524
Fragments arranged by size:
2159 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2058 2111 2292 2411 2525 2567 2751 2058
Size: 53 181 119 114 42 184 5035
Fragments arranged by size:
5035 184 181 119 114 53 42
EcoRI G'AATT_C
Cuts at: 3037 3415 3037
Size: 378 5350
EcoRV GAT'ATC
Cuts at: 2992 2992
Size: 5728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 5728
FspI TGC'GCA
Cuts at: 4234 4234
Size: 5728
HaeII r_GCGC'y
Cuts at: 1678 4875 1678
Size: 3197 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 4369 4401 4443
Size: 32 135 32 656 32 2109 32 42
Cuts at: 4443 4475 1373
Size: 32 2626
Fragments arranged by size:
2626 2109 656 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2536 3072 3318 669
Size: 988 281 598 536 246 3079
Fragments arranged by size:
3079 988 598 536 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5728
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KpnI G_GTAC'C
Cuts at: 3053 3053
Size: 5728
MfeI C'AATT_G
Cuts at: 3327 3327
Size: 5728
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4842 5026 1305
Size: 3537 184 2007
Fragments arranged by size:
3537 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2065 10
Size: 54 1425 343 233 3673
Fragments arranged by size:
3673 1425 343 233 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2235 2626 2736 3686 4045 4204 518
Size: 648 1069 391 110 950 359 159 2042
Fragments arranged by size:
2042 1069 950 648 391 359 159 110
MspA1I CmG'CkG
Cuts at: 1606 3144 3821 4969 5214 1606
Size: 1538 677 1148 245 2120
Fragments arranged by size:
2120 1538 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5176
NdeI CA'TA_TG
Cuts at: 387 2911 387
Size: 2524 3204
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 3175 3175
Size: 5728
NspI r_CATG'y
Cuts at: 1132 1229 2476 2741 3086 4631 1132
Size: 97 1247 265 345 1545 2229
Fragments arranged by size:
2229 1545 1247 345 265 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5728
PciI A'CATG_T
Cuts at: 2472 4627 2472
Size: 2155 3573
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5728
PfoI T'CCnGG_A
Cuts at: 2456 2456
Size: 5728
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 5310 5342 3797
Size: 32 1481 32 4183
Fragments arranged by size:
4183 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2111 2292 2751 2111
Size: 181 459 5088
Fragments arranged by size:
5088 459 181
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5728
PsiI TTA'TAA
Cuts at: 3298 3298
Size: 5728
PspOMI G'GGCC_C
Cuts at: 2410 2525 2566 2410
Size: 115 41 5572
Fragments arranged by size:
5572 115 41
PspXI vC'TCGA_Gb
Cuts at: 2638 2638
Size: 5728
PstI C_TGCA'G
Cuts at: 830 2055 2453 2524 2854 3080 830
Size: 1225 398 71 330 226 3478
Fragments arranged by size:
3478 1225 398 330 226 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
SacI G_AGCT'C
Cuts at: 2465 2465
Size: 5728
SalI G'TCGA_C
Cuts at: 1936 3070 1936
Size: 1134 4594
SbfI CC_TGCA'GG
Cuts at: 2854 3080 2854
Size: 226 5502
ScaI AGT'ACT
Cuts at: 3976 5688 3976
Size: 1712 4016
SfcI C'TryA_G
Cuts at: 826 1045 2051 2296 2449 2520 2590 2850 3076
Size: 219 1006 245 153 71 70 260 226
Cuts at: 3076 4211 4892 5083 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1006 681 260 245 226 219
191 153 71 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 3055 1872
Size: 1183 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2638 3832 4733 4995 820
Size: 197 926 695 1194 901 262 1553
Fragments arranged by size:
1553 1194 926 901 695 262 197
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 3086 1132
Size: 1954 3774
SspI AAT'ATT
Cuts at: 5 52 3652 5
Size: 47 3600 2081
Fragments arranged by size:
3600 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5728
StyI C'CwwG_G
Cuts at: 513 1065 2247 2529 3167 513
Size: 552 1182 282 638 3074
Fragments arranged by size:
3074 1182 638 552 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2121 2255 2591 2710 3755 3772 3925
Size: 1255 541 134 336 119 1045 17 153
Cuts at: 3925 4110 4555 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1045 541 445 336 185 153
134 119 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3000 3974 5686
Size: 274 80 33 51 1443 1023 974 1712
Cuts at: 5686 96
Size: 138
Fragments arranged by size:
1712 1443 1023 974 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 2995 3027 5688 5720 2421
Size: 32 542 32 2661 32 2429
Fragments arranged by size:
2661 2429 542 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2363 2443 1316
Size: 1047 80 4601
Fragments arranged by size:
4601 1047 80
XbaI T'CTAG_A
Cuts at: 3064 3064
Size: 5728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2353 2011
Size: 342 5386
XhoI C'TCGA_G
Cuts at: 1943 2638 1943
Size: 695 5033
XmaI C'CCGG_G
Cuts at: 1870 3053 1870
Size: 1183 4545
XmnI GAAnn'nnTTC
Cuts at: 2089 2674 3857 2089
Size: 585 1183 3960
Fragments arranged by size:
3960 1183 585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3536 276
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI