Restriction map for
pFN21AE4331
(Circular) (Six-base) MAPSORT of: pFN21AE4331.seq Check: 9927 from: 1 to: 5719
pFN21AE4331 5719 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 1937
Size: 1125 4594
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2038 2156 2576 2993 3035 3782 5166 5434
Size: 406 118 420 417 42 747 1384 268
Cuts at: 5434 1632
Size: 1917
Fragments arranged by size:
1917 1384 747 420 417 406 268 118
42
AfeI AGC'GCT
Cuts at: 2035 2035
Size: 5719
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 4618 5595 1203
Size: 3415 977 1327
Fragments arranged by size:
3415 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 2363 2711 1166
Size: 1197 348 4174
Fragments arranged by size:
4174 1197 348
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5719
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2327 2359 2327
Size: 32 5687
AlwNI CAG_nnn'CTG
Cuts at: 2741 3462 5034 2741
Size: 721 1572 3426
Fragments arranged by size:
3426 1572 721
ApaLI G'TGCA_C
Cuts at: 2638 3776 4932 2638
Size: 1138 1156 3425
Fragments arranged by size:
3425 1156 1138
ApoI r'AATT_y
Cuts at: 1493 3028 3254 3406 5515 1493
Size: 1535 226 152 2109 1697
Fragments arranged by size:
2109 1697 1535 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 2574 2966 3044 1870
Size: 73 388 243 392 78 4545
Fragments arranged by size:
4545 392 388 243 78 73
BamHI G'GATC_C
Cuts at: 2566 2691 3049 3416 2566
Size: 125 358 367 4869
Fragments arranged by size:
4869 367 358 125
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2768 3040 4495 618
Size: 325 217 627 981 272 1455 1842
Fragments arranged by size:
1842 1455 981 627 325 272 217
BanII G_rGCy'C
Cuts at: 1391 3038 1391
Size: 1647 4072
BbeI G_GCGC'C
Cuts at: 2772 2772
Size: 5719
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2618 2773 3014 928
Size: 971 719 155 241 3633
Fragments arranged by size:
3633 971 719 241 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3910 3944 1903
Size: 34 50 34 1889 34 3678
Fragments arranged by size:
3678 1889 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2203 5714 2203
Size: 3511 2208
BlpI GC'TnA_GC
Cuts at: 3136 3136
Size: 5719
Bme1580I G_kGCm'C
Cuts at: 1790 2392 2642 3780 4936 1790
Size: 602 250 1138 1156 2573
Fragments arranged by size:
2573 1156 1138 602 250
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2694 3450 4408
Size: 416 53 83 399 24 1277 756 958
Cuts at: 4408 442
Size: 1753
Fragments arranged by size:
1753 1277 958 756 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2124 2325 4379 1239
Size: 885 201 2054 2579
Fragments arranged by size:
2579 2054 885 201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3844 4709 5007 3844
Size: 865 298 4556
Fragments arranged by size:
4556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2213 4382 882
Size: 617 714 2169 2219
Fragments arranged by size:
2219 2169 714 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2769 3526 3908 4543
Size: 53 83 186 936 1236 757 382 635
Cuts at: 4543 275
Size: 1451
Fragments arranged by size:
1451 1236 936 757 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2431 4152 4550 4824 4971 5382 2431
Size: 1721 398 274 147 411 2768
Fragments arranged by size:
2768 1721 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5494
Fragments arranged by size:
5494 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2920 2954 1186
Size: 1734 34 3951
Fragments arranged by size:
3951 1734 34
BseYI C'CCAG_C
Cuts at: 2391 2606 4922 5418 2391
Size: 215 2316 496 2692
Fragments arranged by size:
2692 2316 496 215
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5146
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3930 4958 1540
Size: 275 186 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2642 3780 3865 4936 2642
Size: 1138 85 1071 3425
Fragments arranged by size:
3425 1138 1071 85
BsmI GAATG_Cn'
Cuts at: 3230 3323 3230
Size: 93 5626
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2392 2642 3038 3780 3865 4936 1391
Size: 399 602 250 396 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 602 399 396 250 85
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3074 844
Size: 2230 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2421 2805 4214 4388 65
Size: 1183 1173 384 1409 174 1396
Fragments arranged by size:
1409 1396 1183 1173 384 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2702 2765 4363 4550 1700
Size: 110 892 63 1598 187 2869
Fragments arranged by size:
2869 1598 892 187 110 63
BsrGI T'GTAC_A
Cuts at: 96 2484 96
Size: 2388 3331
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2741 794
Size: 1947 3772
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 2365
Size: 5719
BstYI r'GATC_y
Cuts at: 1972 2203 2566 2691 3049 3416 3801 3818 5270
Size: 231 363 125 358 367 385 17 1452
Cuts at: 5270 5356 5368 5714 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 363 358 346 231
125 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2754 513
Size: 552 1689 3478
Fragments arranged by size:
3478 1689 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 2395 3235 4029 4049 2395
Size: 840 794 20 4065
Fragments arranged by size:
4065 840 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2367 2599
Size: 54 1425 50 275 18 366 171 232
Cuts at: 2599 3166 4055 5684 8
Size: 567 889 1629 43
Fragments arranged by size:
1629 1425 889 567 366 275 232 171
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3649 1353
Size: 2296 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2002 2033 2704 4306 4680 4826
Size: 1064 138 413 31 671 1602 374 146
Cuts at: 4826 387
Size: 1280
Fragments arranged by size:
1602 1280 1064 671 413 374 146 138
31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2124 2156 2325 2576 2993 3035
Size: 393 406 86 32 169 251 417 42
Cuts at: 3035 3782 4379 5166 5434 1239
Size: 747 597 787 268 1524
Fragments arranged by size:
1524 787 747 597 417 406 393 268
251 169 86 42 32
EcoO109I rG'GnC_Cy
Cuts at: 2097 2097
Size: 5719
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
EcoRV GAT'ATC
Cuts at: 2176 2176
Size: 5719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 5719
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 2037 2772 4866 1678
Size: 359 735 2094 2531
Fragments arranged by size:
2531 2094 735 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2568 2600 4360 4392 4434
Size: 32 135 32 996 32 1760 32 42
Cuts at: 4434 4466 1373
Size: 32 2626
Fragments arranged by size:
2626 1760 996 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2581 3063 3309 669
Size: 988 281 643 482 246 3079
Fragments arranged by size:
3079 988 643 482 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KasI G'GCGC_C
Cuts at: 2768 2768
Size: 5719
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4833 5017 1305
Size: 3528 184 2007
Fragments arranged by size:
3528 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 2601 10
Size: 54 1425 343 537 232 3128
Fragments arranged by size:
3128 1425 537 343 232 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 2711 3677 4036 4195 518
Size: 648 1197 348 966 359 159 2042
Fragments arranged by size:
2042 1197 966 648 359 348 159
MspA1I CmG'CkG
Cuts at: 1606 2231 2260 2381 2671 2878 3135 3812 4960
Size: 625 29 121 290 207 257 677 1148
Cuts at: 4960 5205 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 625 290 257 245 207
121 29
NaeI GCC'GGC
Cuts at: 2704 2767 2704
Size: 63 5656
NarI GG'CG_CC
Cuts at: 2769 2769
Size: 5719
NcoI C'CATG_G
Cuts at: 513 1065 2754 513
Size: 552 1689 3478
Fragments arranged by size:
3478 1689 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NgoMIV G'CCGG_C
Cuts at: 2702 2765 2702
Size: 63 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 3077 4622 1132
Size: 97 1848 1545 2229
Fragments arranged by size:
2229 1848 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5719
PciI A'CATG_T
Cuts at: 4618 4618
Size: 5719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5719
PfoI T'CCnGG_A
Cuts at: 2694 2694
Size: 5719
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PspXI vC'TCGA_Gb
Cuts at: 2574 2574
Size: 5719
PstI C_TGCA'G
Cuts at: 830 3071 830
Size: 2241 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2260 2671 2260
Size: 411 5308
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SfcI C'TryA_G
Cuts at: 826 1045 3067 4202 4883 5074 826
Size: 219 2022 1135 681 191 1471
Fragments arranged by size:
2022 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2770 2770
Size: 5719
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 2333 3046 1872
Size: 461 713 4545
Fragments arranged by size:
4545 713 461
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2574 3823 4724 4986 820
Size: 197 926 631 1249 901 262 1553
Fragments arranged by size:
1553 1249 926 901 631 262 197
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 2978 1517
Size: 1461 4258
StyI C'CwwG_G
Cuts at: 513 1065 2148 2646 2754 3158 513
Size: 552 1083 498 108 404 3074
Fragments arranged by size:
3074 1083 552 498 404 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3746 3763 3916 4101 4546 325
Size: 1255 2166 17 153 185 445 1498
Fragments arranged by size:
2166 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 3965 5677
Size: 274 80 33 51 1443 507 1481 1712
Cuts at: 5677 96
Size: 138
Fragments arranged by size:
1712 1481 1443 507 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5679 5711 5679
Size: 32 5687
Tth111I GACn'n_nGTC
Cuts at: 1316 2525 1316
Size: 1209 4510
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2752 2752
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 2574 1943
Size: 631 5088
XmaI C'CCGG_G
Cuts at: 1870 2331 3044 1870
Size: 461 713 4545
Fragments arranged by size:
4545 713 461
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI