(Circular) (Six-base) MAPSORT of: pFN21AE4331.seq Check: 9927 from: 1 to: 5719 pFN21AE4331 5719 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3529 278 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3062 1937 Size: 1125 4594 Acc65I G'GTAC_C Cuts at: 3040 3040 Size: 5719 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2038 2156 2576 2993 3035 3782 5166 5434 Size: 406 118 420 417 42 747 1384 268 Cuts at: 5434 1632 Size: 1917 Fragments arranged by size: 1917 1384 747 420 417 406 268 118 42 AfeI AGC'GCT Cuts at: 2035 2035 Size: 5719 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 4618 5595 1203 Size: 3415 977 1327 Fragments arranged by size: 3415 1327 977 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 4448 4448 Size: 5719 AleI CACnn'nnGTG Cuts at: 1166 2363 2711 1166 Size: 1197 348 4174 Fragments arranged by size: 4174 1197 348 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5719 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2327 2359 2327 Size: 32 5687 AlwNI CAG_nnn'CTG Cuts at: 2741 3462 5034 2741 Size: 721 1572 3426 Fragments arranged by size: 3426 1572 721 ApaLI G'TGCA_C Cuts at: 2638 3776 4932 2638 Size: 1138 1156 3425 Fragments arranged by size: 3425 1156 1138 ApoI r'AATT_y Cuts at: 1493 3028 3254 3406 5515 1493 Size: 1535 226 152 2109 1697 Fragments arranged by size: 2109 1697 1535 226 152 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 2331 2574 2966 3044 1870 Size: 73 388 243 392 78 4545 Fragments arranged by size: 4545 392 388 243 78 73 BamHI G'GATC_C Cuts at: 2566 2691 3049 3416 2566 Size: 125 358 367 4869 Fragments arranged by size: 4869 367 358 125 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2768 3040 4495 618 Size: 325 217 627 981 272 1455 1842 Fragments arranged by size: 1842 1455 981 627 325 272 217 BanII G_rGCy'C Cuts at: 1391 3038 1391 Size: 1647 4072 BbeI G_GCGC'C Cuts at: 2772 2772 Size: 5719 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2618 2773 3014 928 Size: 971 719 155 241 3633 Fragments arranged by size: 3633 971 719 241 155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3910 3944 1903 Size: 34 50 34 1889 34 3678 Fragments arranged by size: 3678 1889 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3611 4821 3611 Size: 1210 4509 BclI T'GATC_A Cuts at: 1548 1548 Size: 5719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4330 136 Size: 107 122 71 631 605 2658 1525 Fragments arranged by size: 2658 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2203 5714 2203 Size: 3511 2208 BlpI GC'TnA_GC Cuts at: 3136 3136 Size: 5719 Bme1580I G_kGCm'C Cuts at: 1790 2392 2642 3780 4936 1790 Size: 602 250 1138 1156 2573 Fragments arranged by size: 2573 1156 1138 602 250 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5719 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2694 3450 4408 Size: 416 53 83 399 24 1277 756 958 Cuts at: 4408 442 Size: 1753 Fragments arranged by size: 1753 1277 958 756 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2124 2325 4379 1239 Size: 885 201 2054 2579 Fragments arranged by size: 2579 2054 885 201 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3844 4709 5007 3844 Size: 865 298 4556 Fragments arranged by size: 4556 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2213 4382 882 Size: 617 714 2169 2219 Fragments arranged by size: 2219 2169 714 617 BsaAI yAC'GTr Cuts at: 493 1204 3510 493 Size: 711 2306 2702 Fragments arranged by size: 2702 2306 711 BsaBI GATnn'nnATC Cuts at: 5713 5713 Size: 5719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2769 3526 3908 4543 Size: 53 83 186 936 1236 757 382 635 Cuts at: 4543 275 Size: 1451 Fragments arranged by size: 1451 1236 936 757 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2431 4152 4550 4824 4971 5382 2431 Size: 1721 398 274 147 411 2768 Fragments arranged by size: 2768 1721 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5494 Fragments arranged by size: 5494 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2920 2954 1186 Size: 1734 34 3951 Fragments arranged by size: 3951 1734 34 BseYI C'CCAG_C Cuts at: 2391 2606 4922 5418 2391 Size: 215 2316 496 2692 Fragments arranged by size: 2692 2316 496 215 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5146 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3169 3930 4958 1540 Size: 275 186 1168 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2642 3780 3865 4936 2642 Size: 1138 85 1071 3425 Fragments arranged by size: 3425 1138 1071 85 BsmI GAATG_Cn' Cuts at: 3230 3323 3230 Size: 93 5626 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2392 2642 3038 3780 3865 4936 1391 Size: 399 602 250 396 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 602 399 396 250 85 BspEI T'CCGG_A Cuts at: 5382 5382 Size: 5719 BspHI T'CATG_A Cuts at: 3608 5338 3608 Size: 1730 3989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3074 844 Size: 2230 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3606 1394 Size: 429 1783 3507 Fragments arranged by size: 3507 1783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2421 2805 4214 4388 65 Size: 1183 1173 384 1409 174 1396 Fragments arranged by size: 1409 1396 1183 1173 384 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2702 2765 4363 4550 1700 Size: 110 892 63 1598 187 2869 Fragments arranged by size: 2869 1598 892 187 110 63 BsrGI T'GTAC_A Cuts at: 96 2484 96 Size: 2388 3331 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 3779 4791 3779 Size: 1012 4707 BstAPI GCAn_nnn'nTGC Cuts at: 794 2741 794 Size: 1947 3772 BstBI TT'CG_AA Cuts at: 4523 4523 Size: 5719 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5719 BstXI CCAn_nnnn'nTGG Cuts at: 2365 2365 Size: 5719 BstYI r'GATC_y Cuts at: 1972 2203 2566 2691 3049 3416 3801 3818 5270 Size: 231 363 125 358 367 385 17 1452 Cuts at: 5270 5356 5368 5714 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 363 358 346 231 125 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2754 513 Size: 552 1689 3478 Fragments arranged by size: 3478 1689 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4980 Fragments arranged by size: 4980 683 56 BtsI GCAGTG_nn' Cuts at: 2395 3235 4029 4049 2395 Size: 840 794 20 4065 Fragments arranged by size: 4065 840 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 3023 3023 Size: 5719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2367 2599 Size: 54 1425 50 275 18 366 171 232 Cuts at: 2599 3166 4055 5684 8 Size: 567 889 1629 43 Fragments arranged by size: 1629 1425 889 567 366 275 232 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3166 1537 Size: 275 1354 4090 Fragments arranged by size: 4090 1354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3649 1353 Size: 2296 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2002 2033 2704 4306 4680 4826 Size: 1064 138 413 31 671 1602 374 146 Cuts at: 4826 387 Size: 1280 Fragments arranged by size: 1602 1280 1064 671 413 374 146 138 31 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2124 2156 2325 2576 2993 3035 Size: 393 406 86 32 169 251 417 42 Cuts at: 3035 3782 4379 5166 5434 1239 Size: 747 597 787 268 1524 Fragments arranged by size: 1524 787 747 597 417 406 393 268 251 169 86 42 32 EcoO109I rG'GnC_Cy Cuts at: 2097 2097 Size: 5719 EcoRI G'AATT_C Cuts at: 3028 3406 3028 Size: 378 5341 EcoRV GAT'ATC Cuts at: 2176 2176 Size: 5719 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2671 2671 Size: 5719 FspI TGC'GCA Cuts at: 4225 4225 Size: 5719 HaeII r_GCGC'y Cuts at: 1678 2037 2772 4866 1678 Size: 359 735 2094 2531 Fragments arranged by size: 2531 2094 735 359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2568 2600 4360 4392 4434 Size: 32 135 32 996 32 1760 32 42 Cuts at: 4434 4466 1373 Size: 32 2626 Fragments arranged by size: 2626 1760 996 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2581 3063 3309 669 Size: 988 281 643 482 246 3079 Fragments arranged by size: 3079 988 643 482 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5719 HpaI GTT'AAC Cuts at: 3309 3309 Size: 5719 KasI G'GCGC_C Cuts at: 2768 2768 Size: 5719 KpnI G_GTAC'C Cuts at: 3044 3044 Size: 5719 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4833 5017 1305 Size: 3528 184 2007 Fragments arranged by size: 3528 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2369 2601 10 Size: 54 1425 343 537 232 3128 Fragments arranged by size: 3128 1425 537 343 232 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 2711 3677 4036 4195 518 Size: 648 1197 348 966 359 159 2042 Fragments arranged by size: 2042 1197 966 648 359 348 159 MspA1I CmG'CkG Cuts at: 1606 2231 2260 2381 2671 2878 3135 3812 4960 Size: 625 29 121 290 207 257 677 1148 Cuts at: 4960 5205 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 625 290 257 245 207 121 29 NaeI GCC'GGC Cuts at: 2704 2767 2704 Size: 63 5656 NarI GG'CG_CC Cuts at: 2769 2769 Size: 5719 NcoI C'CATG_G Cuts at: 513 1065 2754 513 Size: 552 1689 3478 Fragments arranged by size: 3478 1689 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NgoMIV G'CCGG_C Cuts at: 2702 2765 2702 Size: 63 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 3077 4622 1132 Size: 97 1848 1545 2229 Fragments arranged by size: 2229 1848 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5719 PciI A'CATG_T Cuts at: 4618 4618 Size: 5719 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5719 PfoI T'CCnGG_A Cuts at: 2694 2694 Size: 5719 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PspXI vC'TCGA_Gb Cuts at: 2574 2574 Size: 5719 PstI C_TGCA'G Cuts at: 830 3071 830 Size: 2241 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 PvuII CAG'CTG Cuts at: 2260 2671 2260 Size: 411 5308 SalI G'TCGA_C Cuts at: 1936 3061 1936 Size: 1125 4594 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 3967 5679 3967 Size: 1712 4007 SfcI C'TryA_G Cuts at: 826 1045 3067 4202 4883 5074 826 Size: 219 2022 1135 681 191 1471 Fragments arranged by size: 2022 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2770 2770 Size: 5719 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5719 SmaI CCC'GGG Cuts at: 1872 2333 3046 1872 Size: 461 713 4545 Fragments arranged by size: 4545 713 461 SmlI C'TyrA_G Cuts at: 820 1017 1943 2574 3823 4724 4986 820 Size: 197 926 631 1249 901 262 1553 Fragments arranged by size: 1553 1249 926 901 631 262 197 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 3077 1132 Size: 1945 3774 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 2978 1517 Size: 1461 4258 StyI C'CwwG_G Cuts at: 513 1065 2148 2646 2754 3158 513 Size: 552 1083 498 108 404 3074 Fragments arranged by size: 3074 1083 552 498 404 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3746 3763 3916 4101 4546 325 Size: 1255 2166 17 153 185 445 1498 Fragments arranged by size: 2166 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2484 3965 5677 Size: 274 80 33 51 1443 507 1481 1712 Cuts at: 5677 96 Size: 138 Fragments arranged by size: 1712 1481 1443 507 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5679 5711 5679 Size: 32 5687 Tth111I GACn'n_nGTC Cuts at: 1316 2525 1316 Size: 1209 4510 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2752 2752 Size: 5719 XhoI C'TCGA_G Cuts at: 1943 2574 1943 Size: 631 5088 XmaI C'CCGG_G Cuts at: 1870 2331 3044 1870 Size: 461 713 4545 Fragments arranged by size: 4545 713 461 XmnI GAAnn'nnTTC Cuts at: 3848 3848 Size: 5719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3527 276 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI