Restriction map for
pFN21AE4327
(Circular) (Six-base) MAPSORT of: pFN21AE4327.seq Check: 7011 from: 1 to: 5701
pFN21AE4327 5701 bp
With 182 enzymes: SgfI *
March 28, 2012 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2288 2288
Size: 5701
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3044 1937
Size: 1107 4594
Acc65I G'GTAC_C
Cuts at: 3022 3022
Size: 5701
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3764 5148 5416 1632
Size: 2132 1384 268 1917
Fragments arranged by size:
2132 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 4600 5577 1203
Size: 3397 977 1327
Fragments arranged by size:
3397 1327 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 4430 4430
Size: 5701
AleI CACnn'nnGTG
Cuts at: 1166 2388 2419 1166
Size: 1222 31 4448
Fragments arranged by size:
4448 1222 31
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2437 1184
Size: 1253 4448
AlwNI CAG_nnn'CTG
Cuts at: 2044 2352 3444 5016 2044
Size: 308 1092 1572 2729
Fragments arranged by size:
2729 1572 1092 308
ApaI G_GGCC'C
Cuts at: 2124 2982 2124
Size: 858 4843
ApaLI G'TGCA_C
Cuts at: 2412 3758 4914 2412
Size: 1346 1156 3199
Fragments arranged by size:
3199 1346 1156
ApoI r'AATT_y
Cuts at: 1493 3010 3236 3388 5497 1493
Size: 1517 226 152 2109 1697
Fragments arranged by size:
2109 1697 1517 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 3026 1870
Size: 73 1083 4545
Fragments arranged by size:
4545 1083 73
BamHI G'GATC_C
Cuts at: 3031 3398 3031
Size: 367 5334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 3022 4477 618
Size: 325 217 627 396 839 1455 1842
Fragments arranged by size:
1842 1455 839 627 396 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 2345 2982 3020 1391
Size: 733 221 637 38 4072
Fragments arranged by size:
4072 733 637 221 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4730
BbvCI CC'TCA_GC
Cuts at: 2125 2125
Size: 5701
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3892 3926 1903
Size: 34 50 34 1871 34 3678
Fragments arranged by size:
3678 1871 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4803 3593
Size: 1210 4491
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5701
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2986 4312 136
Size: 107 122 71 631 605 1314 1326 1525
Fragments arranged by size:
1525 1326 1314 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5696 5696
Size: 5701
BlpI GC'TnA_GC
Cuts at: 3118 3118
Size: 5701
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2186 2416 2982 3762 4918 1790
Size: 334 62 230 566 780 1156 2573
Fragments arranged by size:
2573 1156 780 566 334 230 62
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2125 3432 4390
Size: 416 53 83 399 24 708 1307 958
Cuts at: 4390 442
Size: 1753
Fragments arranged by size:
1753 1307 958 708 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2329 2596 2792 4361 1239
Size: 1090 267 196 1569 2579
Fragments arranged by size:
2579 1569 1090 267 196
Bpu10I CC'TnA_GC
Cuts at: 2125 2125
Size: 5701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3826 4691 4989 3826
Size: 865 298 4538
Fragments arranged by size:
4538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4364 882
Size: 617 2865 2219
Fragments arranged by size:
2865 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3492 493
Size: 711 2288 2702
Fragments arranged by size:
2702 2288 711
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3508 3890 4525 275
Size: 53 83 186 936 1975 382 635 1451
Fragments arranged by size:
1975 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4134 4532 4806 4953 5364 4134
Size: 398 274 147 411 4471
Fragments arranged by size:
4471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2223 2253 2569 2599 1178
Size: 30 165 30 820 30 316 30 4280
Fragments arranged by size:
4280 820 316 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2804 2807 1186
Size: 1162 456 3 4080
Fragments arranged by size:
4080 1162 456 3
BseYI C'CCAG_C
Cuts at: 2332 2664 4904 5400 2332
Size: 332 2240 496 2633
Fragments arranged by size:
2633 2240 496 332
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2082 2197 1163
Size: 573 346 115 4667
Fragments arranged by size:
4667 573 346 115
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3151 3912 4940 1540
Size: 275 186 1150 761 1028 2301
Fragments arranged by size:
2301 1150 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2416 2444 3762 3847 4918 2416
Size: 28 1318 85 1071 3199
Fragments arranged by size:
3199 1318 1071 85 28
BsmI GAATG_Cn'
Cuts at: 3212 3305 3212
Size: 93 5608
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2186 2345 2416 2444 2982 3020
Size: 399 334 62 159 71 28 538 38
Cuts at: 3020 3762 3847 4918 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 538 399 334 159 85
71 62 38 28
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 3590 5320 3590
Size: 1730 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2288 2490 2534 3056 844
Size: 1444 202 44 522 3489
Fragments arranged by size:
3489 1444 522 202 44
BsrBI CCG'CTC
Cuts at: 1394 1823 3588 1394
Size: 429 1765 3507
Fragments arranged by size:
3507 1765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4196 4370 65
Size: 1183 2948 174 1396
Fragments arranged by size:
2948 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2989 4345 4532 1700
Size: 110 1179 1356 187 2869
Fragments arranged by size:
2869 1356 1179 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5701
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5701
BssSI C'ACGA_G
Cuts at: 2443 2539 3761 4773 2443
Size: 96 1222 1012 3371
Fragments arranged by size:
3371 1222 1012 96
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2411 794
Size: 1617 4084
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 2689 1184
Size: 1505 4196
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2374 2011
Size: 363 5338
BstYI r'GATC_y
Cuts at: 1972 3031 3398 3783 3800 5252 5338 5350 5696
Size: 1059 367 385 17 1452 86 12 346
Cuts at: 5696 1972
Size: 1977
Fragments arranged by size:
1977 1452 1059 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2168 2432 2168
Size: 264 5437
BtgI C'CryG_G
Cuts at: 513 1065 2062 2982 513
Size: 552 997 920 3232
Fragments arranged by size:
3232 997 920 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4962
Fragments arranged by size:
4962 683 56
BtsI GCAGTG_nn'
Cuts at: 2042 2412 2444 2478 2560 3217 4011 4031 2042
Size: 370 32 34 82 657 794 20 3712
Fragments arranged by size:
3712 794 657 370 82 34 32 20
ClaI AT'CG_AT
Cuts at: 1554 2307 1554
Size: 753 4948
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5666
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4708 809
Size: 507 3392 1802
Fragments arranged by size:
3392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2557 2763 2885
Size: 54 1425 50 275 18 727 206 122
Cuts at: 2885 3148 4037 5666 8
Size: 263 889 1629 43
Fragments arranged by size:
1629 1425 889 727 275 263 206 122
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3148 1537
Size: 275 1336 4090
Fragments arranged by size:
4090 1336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2366 2640 3631 1353
Size: 1013 274 991 3423
Fragments arranged by size:
3423 1013 991 274
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4288 4662 4808 387
Size: 1064 138 2699 374 146 1280
Fragments arranged by size:
2699 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2329 2596 2792 3764 4361 5148 5416
Size: 393 697 267 196 972 597 787 268
Cuts at: 5416 1239
Size: 1524
Fragments arranged by size:
1524 972 787 697 597 393 268 267
196
EcoO109I rG'GnC_Cy
Cuts at: 2120 2121 2164 2861 2978 2120
Size: 1 43 697 117 4843
Fragments arranged by size:
4843 697 117 43 1
EcoRI G'AATT_C
Cuts at: 3010 3388 3010
Size: 378 5323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2412 2412
Size: 5701
FspI TGC'GCA
Cuts at: 4207 4207
Size: 5701
HaeII r_GCGC'y
Cuts at: 1678 4848 1678
Size: 3170 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2223 2255 2569 2601 4342
Size: 32 135 32 651 32 314 32 1741
Cuts at: 4342 4374 4416 4448 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1741 651 314 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3045 3291 669
Size: 988 281 1107 246 3079
Fragments arranged by size:
3079 1107 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5701
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KpnI G_GTAC'C
Cuts at: 3026 3026
Size: 5701
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4815 4999 1305
Size: 3510 184 2007
Fragments arranged by size:
3510 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2559 2765 2887 10
Size: 54 1425 343 727 206 122 2824
Fragments arranged by size:
2824 1425 727 343 206 122 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2388 2419 3659 4018 4177 518
Size: 648 1222 31 1240 359 159 2042
Fragments arranged by size:
2042 1240 1222 648 359 159 31
MspA1I CmG'CkG
Cuts at: 1606 3117 3794 4942 5187 1606
Size: 1511 677 1148 245 2120
Fragments arranged by size:
2120 1511 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5149
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 3148 3148
Size: 5701
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5701
NspI r_CATG'y
Cuts at: 1132 1229 2487 3059 4604 1132
Size: 97 1258 572 1545 2229
Fragments arranged by size:
2229 1545 1258 572 97
PasI CC'CwG_GG
Cuts at: 1348 2877 1348
Size: 1529 4172
PciI A'CATG_T
Cuts at: 4600 4600
Size: 5701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2352 1108
Size: 1244 4457
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 5283 5315 3770
Size: 32 1481 32 4156
Fragments arranged by size:
4156 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 3271 3271
Size: 5701
PspOMI G'GGCC_C
Cuts at: 2120 2978 2120
Size: 858 4843
PstI C_TGCA'G
Cuts at: 830 2052 3053 830
Size: 1222 1001 3478
Fragments arranged by size:
3478 1222 1001
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
SalI G'TCGA_C
Cuts at: 1936 3043 1936
Size: 1107 4594
SbfI CC_TGCA'GG
Cuts at: 3053 3053
Size: 5701
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SfcI C'TryA_G
Cuts at: 826 1045 2048 3049 4184 4865 5056 826
Size: 219 1003 1001 1135 681 191 1471
Fragments arranged by size:
1471 1135 1003 1001 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2986 2986
Size: 5701
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 3028 1872
Size: 1156 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3805 4706 4968 820
Size: 197 926 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 3059 1132
Size: 1927 3774
SspI AAT'ATT
Cuts at: 5 52 3625 5
Size: 47 3573 2081
Fragments arranged by size:
3573 2081 47
StuI AGG'CCT
Cuts at: 1517 2651 1517
Size: 1134 4567
StyI C'CwwG_G
Cuts at: 513 1065 2151 2261 2592 3140 513
Size: 552 1086 110 331 548 3074
Fragments arranged by size:
3074 1086 552 548 331 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3728 3745 3898 4083 4528 325
Size: 1255 2148 17 153 185 445 1498
Fragments arranged by size:
2148 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3947 5659 96
Size: 274 80 33 51 1443 1970 1712 138
Fragments arranged by size:
1970 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5661 5693 5661
Size: 32 5669
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 2267 3037 2267
Size: 770 4931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2485 2880 2485
Size: 395 5306
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5701
XmaI C'CCGG_G
Cuts at: 1870 3026 1870
Size: 1156 4545
XmnI GAAnn'nnTTC
Cuts at: 3830 3830
Size: 5701
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfoI SrfI SwaI