Restriction map for
pFN21AE4317
(Circular) (Six-base) MAPSORT of: pFN21AE4317.seq Check: 7028 from: 1 to: 5674
pFN21AE4317 5674 bp
With 182 enzymes: SgfI *
March 28, 2012 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2749 2749
Size: 5674
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2372 3484 278
Size: 53 83 186 936 836 1112 2468
Fragments arranged by size:
2468 1112 936 836 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2196 3017 1937
Size: 259 821 4594
Fragments arranged by size:
4594 821 259
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3737 5121 5389 1632
Size: 2105 1384 268 1917
Fragments arranged by size:
2105 1917 1384 268
AfeI AGC'GCT
Cuts at: 2552 2552
Size: 5674
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 2108 2628 4573 5550 1203
Size: 905 520 1945 977 1327
Fragments arranged by size:
1945 1327 977 905 520
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 2270 1166
Size: 1104 4570
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2288 1184
Size: 1104 4570
AlwNI CAG_nnn'CTG
Cuts at: 3417 4989 3417
Size: 1572 4102
ApaI G_GGCC'C
Cuts at: 2018 2018
Size: 5674
ApaLI G'TGCA_C
Cuts at: 2585 3731 4887 2585
Size: 1146 1156 3372
Fragments arranged by size:
3372 1156 1146
ApoI r'AATT_y
Cuts at: 1493 2983 3209 3361 5470 1493
Size: 1490 226 152 2109 1697
Fragments arranged by size:
2109 1697 1490 226 152
AscI GG'CGCG_CC
Cuts at: 2351 2351
Size: 5674
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2182 2333 2999 1870
Size: 73 67 172 151 666 4545
Fragments arranged by size:
4545 666 172 151 73 67
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2498 2995 4450 618
Size: 325 217 627 711 497 1455 1842
Fragments arranged by size:
1842 1455 711 627 497 325 217
BanII G_rGCy'C
Cuts at: 1391 2018 2183 2993 1391
Size: 627 165 810 4072
Fragments arranged by size:
4072 810 627 165
BbeI G_GCGC'C
Cuts at: 2502 2502
Size: 5674
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4703
BbvCI CC'TCA_GC
Cuts at: 2531 2531
Size: 5674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2176 2210 3865 3899 1903
Size: 34 239 34 1655 34 3678
Fragments arranged by size:
3678 1655 239 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 2892 1548
Size: 1344 4330
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2145 2154 2724
Size: 107 122 71 631 605 473 9 570
Cuts at: 2724 2879 4285 136
Size: 155 1406 1525
Fragments arranged by size:
1525 1406 631 605 570 473 155 122
107 71 9
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2589 3735 4891 1790
Size: 228 571 1146 1156 2573
Fragments arranged by size:
2573 1156 1146 571 228
BmgBI CAC'GTC
Cuts at: 1318 2631 2653 1318
Size: 1313 22 4339
Fragments arranged by size:
4339 1313 22
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2954 3405 4363
Size: 416 53 83 399 24 1537 451 958
Cuts at: 4363 442
Size: 1753
Fragments arranged by size:
1753 1537 958 451 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4334 1239
Size: 3095 2579
Bpu10I CC'TnA_GC
Cuts at: 2426 2531 2426
Size: 105 5569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2216 3799 4664 4962 2216
Size: 1583 865 298 2928
Fragments arranged by size:
2928 1583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4337 882
Size: 617 2838 2219
Fragments arranged by size:
2838 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2109 3465 493
Size: 711 905 1356 2702
Fragments arranged by size:
2702 1356 905 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2369 2499 2523 3481
Size: 53 83 186 936 836 130 24 958
Cuts at: 3481 3863 4498 275
Size: 382 635 1451
Fragments arranged by size:
1451 958 936 836 635 382 186 130
83 53 24
BsaWI w'CCGG_w
Cuts at: 2901 4107 4505 4779 4926 5337 2901
Size: 1206 398 274 147 411 3238
Fragments arranged by size:
3238 1206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5449
Fragments arranged by size:
5449 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5674
BseYI C'CCAG_C
Cuts at: 2176 4877 5373 2176
Size: 2701 496 2477
Fragments arranged by size:
2701 2477 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2089 2155 2546 1163
Size: 573 353 66 391 4291
Fragments arranged by size:
4291 573 391 353 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2091 2158 2196 2279 2583 3124
Size: 275 186 90 67 38 83 304 541
Cuts at: 3124 3885 4913 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 541 304 275 186 90
83 67 38
BsiHKAI G_wGCw'C
Cuts at: 2289 2589 3735 3820 4891 2289
Size: 300 1146 85 1071 3072
Fragments arranged by size:
3072 1146 1071 300 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
BsmBI CGTCTCn'nnnn_
Cuts at: 2637 2637
Size: 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2183 2289 2589 2993 3735 3820
Size: 399 228 165 106 300 404 742 85
Cuts at: 3820 4891 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 404 399 300 228 165
106 85
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2484 2749 3029 844
Size: 1640 265 280 3489
Fragments arranged by size:
3489 1640 280 265
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 2495 2645 2828 2873 4318 4505
Size: 110 268 417 150 183 45 1445 187
Cuts at: 4505 1700
Size: 2869
Fragments arranged by size:
2869 1445 417 268 187 183 150 110
45
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 2172 2237 2351 1925
Size: 247 65 114 5248
Fragments arranged by size:
5248 247 114 65
BssSI C'ACGA_G
Cuts at: 2294 3734 4746 2294
Size: 1440 1012 3222
Fragments arranged by size:
3222 1440 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 2074 1184
Size: 890 4784
BstXI CCAn_nnnn'nTGG
Cuts at: 2575 2575
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 3004 3371 3756 3773 5225 5311 5323 5669
Size: 1032 367 385 17 1452 86 12 346
Cuts at: 5669 1972
Size: 1977
Fragments arranged by size:
1977 1452 1032 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2719 513
Size: 552 939 715 3468
Fragments arranged by size:
3468 939 715 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2454 2679 2821 487
Size: 683 56 1228 225 142 3340
Fragments arranged by size:
3340 1228 683 225 142 56
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2088 2155
Size: 54 1425 50 275 18 250 8 67
Cuts at: 2155 2276 2580 2880 3121 4010 5639 8
Size: 121 304 300 241 889 1629 43
Fragments arranged by size:
1629 1425 889 304 300 275 250 241
121 67 54 50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 2088 2155 2276 2580 3121 1537
Size: 275 276 67 121 304 541 4090
Fragments arranged by size:
4090 541 304 276 275 121 67
EarI CTCTTCn'nnn_
Cuts at: 1353 2546 3604 1353
Size: 1193 1058 3423
Fragments arranged by size:
3423 1193 1058
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3737 4334 5121 5389 1239
Size: 393 2105 597 787 268 1524
Fragments arranged by size:
2105 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2014 2015 2014
Size: 1 5673
EcoRI G'AATT_C
Cuts at: 2983 3361 2983
Size: 378 5296
EcoRV GAT'ATC
Cuts at: 2659 2659
Size: 5674
FspI TGC'GCA
Cuts at: 2453 4180 2453
Size: 1727 3947
FspAI rTGC'GCAy
Cuts at: 2453 2453
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 2250 2502 2554 2821 4821 1678
Size: 572 252 52 267 2000 2531
Fragments arranged by size:
2531 2000 572 267 252 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4315 4347 4389 4421 1373
Size: 32 135 32 2743 32 42 32 2626
Fragments arranged by size:
2743 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2197 3018 3264 669
Size: 988 281 259 821 246 3079
Fragments arranged by size:
3079 988 821 281 259 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5674
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KasI G'GCGC_C
Cuts at: 2498 2498
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4788 4972 1305
Size: 3483 184 2007
Fragments arranged by size:
3483 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3852
Fragments arranged by size:
3852 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 2849 3632 3991 4150 518
Size: 648 1104 579 783 359 159 2042
Fragments arranged by size:
2042 1104 783 648 579 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2050 2160 2721 3090 3767 4915 5160
Size: 432 12 110 561 369 677 1148 245
Cuts at: 5160 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 561 432 369 245 110
12
NaeI GCC'GGC
Cuts at: 2497 2830 2875 2497
Size: 333 45 5296
Fragments arranged by size:
5296 333 45
NarI GG'CG_CC
Cuts at: 2499 2499
Size: 5674
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4183
Fragments arranged by size:
4183 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NgoMIV G'CCGG_C
Cuts at: 2495 2828 2873 2495
Size: 333 45 5296
Fragments arranged by size:
5296 333 45
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 2155 3121 2155
Size: 966 4708
NspI r_CATG'y
Cuts at: 1132 1229 3032 4577 1132
Size: 97 1803 1545 2229
Fragments arranged by size:
2229 1803 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PmlI CAC'GTG
Cuts at: 2109 2109
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PspOMI G'GGCC_C
Cuts at: 2014 2014
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2760 3026 830
Size: 1930 266 3478
Fragments arranged by size:
3478 1930 266
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
SacII CC_GC'GG
Cuts at: 2722 2722
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 2195 3016 1936
Size: 259 821 4594
Fragments arranged by size:
4594 821 259
SapI GCTCTTCn'nnn_
Cuts at: 2546 2546
Size: 5674
SbfI CC_TGCA'GG
Cuts at: 2760 3026 2760
Size: 266 5408
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SfcI C'TryA_G
Cuts at: 826 1045 2756 3022 4157 4838 5029 826
Size: 219 1711 266 1135 681 191 1471
Fragments arranged by size:
1711 1471 1135 681 266 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2154 2724 2154
Size: 570 5104
SfoI GGC'GCC
Cuts at: 2500 2500
Size: 5674
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 2495 2645 1700
Size: 795 150 4729
Fragments arranged by size:
4729 795 150
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2231 3778 4679 4941 820
Size: 197 926 288 1547 901 262 1553
Fragments arranged by size:
1553 1547 926 901 288 262 197
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2004 3113 513
Size: 552 939 1109 3074
Fragments arranged by size:
3074 1109 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2471 3701 3718 3871 4056 4501 325
Size: 1255 891 1230 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1230 891 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2919 3920 5632
Size: 274 80 33 51 1443 942 1001 1712
Cuts at: 5632 96
Size: 138
Fragments arranged by size:
1712 1443 1001 942 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2370 3482 276
Size: 53 83 186 936 836 1112 2468
Fragments arranged by size:
2468 1112 936 836 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FalI FseI NruI NsiI PacI
PfoI PpuMI PspXI PsrI PvuII RsrII SacI SanDI
SexAI SrfI SwaI XcmI