Restriction map for
pFN21AE4304
(Circular) (Six-base) MAPSORT of: pFN21AE4304.seq Check: 1221 from: 1 to: 5608
pFN21AE4304 5608 bp
With 182 enzymes: SgfI *
March 28, 2012 14:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 3671 5055 5323 1632
Size: 767 1272 1384 268 1917
Fragments arranged by size:
1917 1384 1272 767 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2219 1184
Size: 1035 4573
AlwNI CAG_nnn'CTG
Cuts at: 2250 2497 3351 4923 2250
Size: 247 854 1572 2935
Fragments arranged by size:
2935 1572 854 247
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2369 2710 2775 2917 3143 3295 5404 1493
Size: 876 341 65 142 226 152 2109 1697
Fragments arranged by size:
2109 1697 876 341 226 152 142 65
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2933 1870
Size: 73 990 4545
Fragments arranged by size:
4545 990 73
BamHI G'GATC_C
Cuts at: 2337 2938 3305 2337
Size: 601 367 4640
Fragments arranged by size:
4640 601 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2929 4384 618
Size: 325 217 627 1142 1455 1842
Fragments arranged by size:
1842 1455 1142 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2835 2927 1391
Size: 1444 92 4072
Fragments arranged by size:
4072 1444 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2599 2633 3799 3833 1903
Size: 34 662 34 1166 34 3678
Fragments arranged by size:
3678 1166 662 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4219 136
Size: 107 122 71 631 605 2547 1525
Fragments arranged by size:
2547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5603 5603
Size: 5608
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 2154 3669 4825 1790
Size: 364 1515 1156 2573
Fragments arranged by size:
2573 1515 1156 364
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2507 3339 4297
Size: 416 53 83 399 24 1090 832 958
Cuts at: 4297 442
Size: 1753
Fragments arranged by size:
1753 1090 958 832 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2610 4268 1239
Size: 1371 1658 2579
Fragments arranged by size:
2579 1658 1371
Bpu10I CC'TnA_GC
Cuts at: 2207 2207
Size: 5608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3733 4598 4896 3733
Size: 865 298 4445
Fragments arranged by size:
4445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3399 493
Size: 711 2195 2702
Fragments arranged by size:
2702 2195 711
BsaBI GATnn'nnATC
Cuts at: 2342 2517 5602 2342
Size: 175 3085 2348
Fragments arranged by size:
3085 2348 175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3415 3797 4432 275
Size: 53 83 186 936 1882 382 635 1451
Fragments arranged by size:
1882 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4041 4439 4713 4860 5271 4041
Size: 398 274 147 411 4378
Fragments arranged by size:
4378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5383
Fragments arranged by size:
5383 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2843 1186
Size: 1657 3951
BseYI C'CCAG_C
Cuts at: 2128 2160 2479 2636 4811 5307 2128
Size: 32 319 157 2175 496 2429
Fragments arranged by size:
2429 2175 496 319 157 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2397 1163
Size: 573 661 4374
Fragments arranged by size:
4374 661 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 3669 3754 4825 2208
Size: 1461 85 1071 2991
Fragments arranged by size:
2991 1461 1071 85
BsmI GAATG_Cn'
Cuts at: 2107 3119 3212 2107
Size: 1012 93 4503
Fragments arranged by size:
4503 1012 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2208 2835 2927 3669 3754 4825
Size: 399 364 54 627 92 742 85 1071
Cuts at: 4825 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 627 399 364 92 85
54
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2963 844
Size: 2119 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2087 3495 1394
Size: 429 264 1408 3507
Fragments arranged by size:
3507 1408 429 264
BsrDI GCAATG_nn'
Cuts at: 65 1248 2907 4103 4277 65
Size: 1183 1659 1196 174 1396
Fragments arranged by size:
1659 1396 1196 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4252 4439 1700
Size: 110 2442 187 2869
Fragments arranged by size:
2869 2442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 2147 3668 4680 2147
Size: 1521 1012 3075
Fragments arranged by size:
3075 1521 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5608
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 2404 1184
Size: 1220 4388
BstYI r'GATC_y
Cuts at: 1972 2322 2337 2628 2938 3305 3690 3707 5159
Size: 350 15 291 310 367 385 17 1452
Cuts at: 5159 5245 5257 5603 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 350 346 310 291
86 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 2746 2746
Size: 5608
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5056
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 2436 3124 3918 3938 2436
Size: 688 794 20 4106
Fragments arranged by size:
4106 794 688 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2541 2568 2730
Size: 54 1425 50 275 18 711 27 162
Cuts at: 2730 3055 3944 5573 8
Size: 325 889 1629 43
Fragments arranged by size:
1629 1425 889 711 325 275 162 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2802 3538 1353
Size: 1449 736 3423
Fragments arranged by size:
3423 1449 736
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 2610 3671 4268 5055 5323 1239
Size: 393 767 211 1061 597 787 268 1524
Fragments arranged by size:
1524 1061 787 767 597 393 268 211
EcoNI CCTnn'n_nnAGG
Cuts at: 2449 2745 2449
Size: 296 5312
EcoO109I rG'GnC_Cy
Cuts at: 2064 2064
Size: 5608
EcoRI G'AATT_C
Cuts at: 2775 2917 3295 2775
Size: 142 378 5088
Fragments arranged by size:
5088 378 142
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 2667 4755 1678
Size: 989 2088 2531
Fragments arranged by size:
2531 2088 989
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2076 2108 2583 2613 2615
Size: 32 135 32 504 32 475 30 2
Cuts at: 2615 2645 4249 4281 4323 4355 1373
Size: 30 1604 32 42 32 2626
Fragments arranged by size:
2626 1604 504 475 135 42 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4722 4906 1305
Size: 3417 184 2007
Fragments arranged by size:
3417 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2543 2570 2732 10
Size: 54 1425 343 711 27 162 2886
Fragments arranged by size:
2886 1425 711 343 162 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 3566 3925 4084 518
Size: 648 2400 359 159 2042
Fragments arranged by size:
2400 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3024 3701 4849 5094 1606
Size: 1418 677 1148 245 2120
Fragments arranged by size:
2120 1418 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5056
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2966 4511 1132
Size: 97 1737 1545 2229
Fragments arranged by size:
2229 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2138 1348
Size: 790 4818
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2250 1108
Size: 1142 4466
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2064 2064
Size: 5608
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PstI C_TGCA'G
Cuts at: 830 2427 2443 2960 830
Size: 1597 16 517 3478
Fragments arranged by size:
3478 1597 517 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2423 2439 2956 4091 4772 4963 826
Size: 219 1378 16 517 1135 681 191 1471
Fragments arranged by size:
1471 1378 1135 681 517 219 191 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3712 4613 4875 820
Size: 197 926 1769 901 262 1553
Fragments arranged by size:
1769 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2400 2450 3047 513
Size: 552 1335 50 597 3074
Fragments arranged by size:
3074 1335 597 552 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 3635 3652 3805 3990 4435 325
Size: 1255 504 1551 17 153 185 445 1498
Fragments arranged by size:
1551 1498 1255 504 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3854 5566 96
Size: 274 80 33 51 1443 1877 1712 138
Fragments arranged by size:
1877 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 2030 1316
Size: 714 4894
XbaI T'CTAG_A
Cuts at: 2561 2944 2561
Size: 383 5225
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 3737 3737
Size: 5608
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI