Restriction map for
pFN21AE4302
(Circular) (Six-base) MAPSORT of: pFN21AE4302.seq Check: 2822 from: 1 to: 5599
pFN21AE4302 5599 bp
With 182 enzymes: SgfI *
March 28, 2012 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2036 2104 2036
Size: 68 5531
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3409 278
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2942 1937
Size: 1005 4594
Acc65I G'GTAC_C
Cuts at: 2920 2920
Size: 5599
AclI AA'CG_TT
Cuts at: 1563 3726 4099 1563
Size: 2163 373 3063
Fragments arranged by size:
3063 2163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3662 5046 5314 1632
Size: 2030 1384 268 1917
Fragments arranged by size:
2030 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5402
AflIII A'CryG_T
Cuts at: 1203 2538 4498 5475 1203
Size: 1335 1960 977 1327
Fragments arranged by size:
1960 1335 1327 977
AgeI A'CCGG_T
Cuts at: 4430 4430
Size: 5599
AhdI GACnn_n'nnGTC
Cuts at: 4328 4328
Size: 5599
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2157 2738 1184
Size: 973 581 4045
Fragments arranged by size:
4045 973 581
AlwNI CAG_nnn'CTG
Cuts at: 3342 4914 3342
Size: 1572 4027
ApaLI G'TGCA_C
Cuts at: 3656 4812 3656
Size: 1156 4443
ApoI r'AATT_y
Cuts at: 1493 2462 2908 3134 3286 5395 1493
Size: 969 446 226 152 2109 1697
Fragments arranged by size:
2109 1697 969 446 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4153 160
Size: 872 3121 1606
Fragments arranged by size:
3121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
AvaI C'yCGr_G
Cuts at: 1870 1943 2924 1870
Size: 73 981 4545
Fragments arranged by size:
4545 981 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2566 2599 2566
Size: 33 5566
BamHI G'GATC_C
Cuts at: 2614 2929 3296 2614
Size: 315 367 4917
Fragments arranged by size:
4917 367 315
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 2920 4375 618
Size: 325 217 627 579 554 1455 1842
Fragments arranged by size:
1842 1455 627 579 554 325 217
BanII G_rGCy'C
Cuts at: 1391 2918 1391
Size: 1527 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3790 3824 1903
Size: 34 1853 34 3678
Fragments arranged by size:
3678 1853 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3491 4701 3491
Size: 1210 4389
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2375 4210 136
Size: 107 122 71 631 605 703 1835 1525
Fragments arranged by size:
1835 1525 703 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2295 2493 5594 2295
Size: 198 3101 2300
Fragments arranged by size:
3101 2300 198
BlpI GC'TnA_GC
Cuts at: 3016 3016
Size: 5599
Bme1580I G_kGCm'C
Cuts at: 1790 3660 4816 1790
Size: 1870 1156 2573
Fragments arranged by size:
2573 1870 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5599
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2320 3330 4288
Size: 416 53 83 399 24 903 1010 958
Cuts at: 4288 442
Size: 1753
Fragments arranged by size:
1753 1010 958 903 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2469 4259 1239
Size: 1230 1790 2579
Fragments arranged by size:
2579 1790 1230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3724 4589 4887 3724
Size: 865 298 4436
Fragments arranged by size:
4436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4262 882
Size: 617 2763 2219
Fragments arranged by size:
2763 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3390 493
Size: 711 2186 2702
Fragments arranged by size:
2702 2186 711
BsaBI GATnn'nnATC
Cuts at: 5593 5593
Size: 5599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3406 3788 4423 275
Size: 53 83 186 936 1873 382 635 1451
Fragments arranged by size:
1873 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2611 4032 4430 4704 4851 5262 2611
Size: 1421 398 274 147 411 2948
Fragments arranged by size:
2948 1421 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5374
Fragments arranged by size:
5374 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5599
BseYI C'CCAG_C
Cuts at: 4802 5298 4802
Size: 496 5103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 2567 1163
Size: 573 402 429 4195
Fragments arranged by size:
4195 573 429 402
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3049 3810 4838 1540
Size: 275 186 1048 761 1028 2301
Fragments arranged by size:
2301 1048 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3660 3745 4816 3660
Size: 85 1071 4443
Fragments arranged by size:
4443 1071 85
BsmI GAATG_Cn'
Cuts at: 2610 3110 3203 2610
Size: 500 93 5006
Fragments arranged by size:
5006 500 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2918 3660 3745 4816 1391
Size: 399 1128 742 85 1071 2174
Fragments arranged by size:
2174 1128 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2611 5262 2611
Size: 2651 2948
BspHI T'CATG_A
Cuts at: 3488 5218 3488
Size: 1730 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2036 2104 2954 844
Size: 1192 68 850 3489
Fragments arranged by size:
3489 1192 850 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2095 3486 1394
Size: 429 272 1391 3507
Fragments arranged by size:
3507 1391 429 272
BsrDI GCAATG_nn'
Cuts at: 65 1248 4094 4268 65
Size: 1183 2846 174 1396
Fragments arranged by size:
2846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4243 4430 1700
Size: 110 2433 187 2869
Fragments arranged by size:
2869 2433 187 110
BsrGI T'GTAC_A
Cuts at: 96 2754 96
Size: 2658 2941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5599
BssSI C'ACGA_G
Cuts at: 2790 3659 4671 2790
Size: 869 1012 3718
Fragments arranged by size:
3718 1012 869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5599
BstBI TT'CG_AA
Cuts at: 4403 4403
Size: 5599
BstEII G'GTnAC_C
Cuts at: 1184 2222 1184
Size: 1038 4561
BstYI r'GATC_y
Cuts at: 1972 2295 2493 2614 2929 3296 3681 3698 5150
Size: 323 198 121 315 367 385 17 1452
Cuts at: 5150 5236 5248 5594 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 323 315 198
121 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5047
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4860
Fragments arranged by size:
4860 683 56
BtsI GCAGTG_nn'
Cuts at: 2139 2592 2664 3115 3909 3929 2139
Size: 453 72 451 794 20 3809
Fragments arranged by size:
3809 794 453 451 72 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5564
DraI TTT'AAA
Cuts at: 2903 2903
Size: 5599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2177 4606 809
Size: 507 861 2429 1802
Fragments arranged by size:
2429 1802 861 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2229 3046
Size: 54 1425 50 275 18 225 174 817
Cuts at: 3046 3935 5564 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 817 275 225 174 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3046 1537
Size: 275 1234 4090
Fragments arranged by size:
4090 1234 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3529 1353
Size: 2176 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2794 4186 4560 4706 387
Size: 1064 138 1205 1392 374 146 1280
Fragments arranged by size:
1392 1280 1205 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2469 3662 4259 5046 5314 1239
Size: 393 837 1193 597 787 268 1524
Fragments arranged by size:
1524 1193 837 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2518 2518
Size: 5599
EcoRI G'AATT_C
Cuts at: 2462 2908 3286 2462
Size: 446 378 4775
Fragments arranged by size:
4775 446 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 5599
FspI TGC'GCA
Cuts at: 4105 4105
Size: 5599
HaeII r_GCGC'y
Cuts at: 1678 2087 2338 4746 1678
Size: 409 251 2408 2531
Fragments arranged by size:
2531 2408 409 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2674 2706 4240 4272 4314
Size: 32 135 32 1102 32 1534 32 42
Cuts at: 4314 4346 1373
Size: 32 2626
Fragments arranged by size:
2626 1534 1102 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2943 3189 669
Size: 988 281 744 261 246 3079
Fragments arranged by size:
3079 988 744 281 261 246
HindIII A'AGCT_T
Cuts at: 748 2808 748
Size: 2060 3539
HpaI GTT'AAC
Cuts at: 3189 3189
Size: 5599
KpnI G_GTAC'C
Cuts at: 2924 2924
Size: 5599
MfeI C'AATT_G
Cuts at: 3198 3198
Size: 5599
MluI A'CGCG_T
Cuts at: 5475 5475
Size: 5599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4713 4897 1305
Size: 3408 184 2007
Fragments arranged by size:
3408 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2231 10
Size: 54 1425 343 399 3378
Fragments arranged by size:
3378 1425 399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2386 2720 3557 3916 4075 518
Size: 648 1220 334 837 359 159 2042
Fragments arranged by size:
2042 1220 837 648 359 334 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2432 2643 3015 3692 4840 5085 1606
Size: 412 414 211 372 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 414 412 372 245 211
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5047
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5599
NotI GC'GGCC_GC
Cuts at: 3046 3046
Size: 5599
NspI r_CATG'y
Cuts at: 1132 1229 2126 2957 4502 1132
Size: 97 897 831 1545 2229
Fragments arranged by size:
2229 1545 897 831 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5599
PciI A'CATG_T
Cuts at: 4498 4498
Size: 5599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5599
PmeI GTTT'AAAC
Cuts at: 2903 2903
Size: 5599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 5181 5213 3668
Size: 32 1481 32 4054
Fragments arranged by size:
4054 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5599
PsiI TTA'TAA
Cuts at: 3169 3169
Size: 5599
PstI C_TGCA'G
Cuts at: 830 2115 2146 2951 830
Size: 1285 31 805 3478
Fragments arranged by size:
3478 1285 805 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5599
PvuII CAG'CTG
Cuts at: 2432 2643 2432
Size: 211 5388
SalI G'TCGA_C
Cuts at: 1936 2941 1936
Size: 1005 4594
SbfI CC_TGCA'GG
Cuts at: 2951 2951
Size: 5599
ScaI AGT'ACT
Cuts at: 3847 5559 3847
Size: 1712 3887
SfcI C'TryA_G
Cuts at: 826 1045 2111 2142 2947 4082 4763 4954 826
Size: 219 1066 31 805 1135 681 191 1471
Fragments arranged by size:
1471 1135 1066 805 681 219 191 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5599
SmaI CCC'GGG
Cuts at: 1872 2926 1872
Size: 1054 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3703 4604 4866 820
Size: 197 926 1760 901 262 1553
Fragments arranged by size:
1760 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3390 493
Size: 2897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5599
SphI G_CATG'C
Cuts at: 1132 2126 2957 1132
Size: 994 831 3774
Fragments arranged by size:
3774 994 831
SspI AAT'ATT
Cuts at: 5 52 3523 5
Size: 47 3471 2081
Fragments arranged by size:
3471 2081 47
StuI AGG'CCT
Cuts at: 1517 2472 1517
Size: 955 4644
StyI C'CwwG_G
Cuts at: 513 1065 2031 2190 2527 3038 513
Size: 552 966 159 337 511 3074
Fragments arranged by size:
3074 966 552 511 337 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 2294 2840 3626 3643 3796 3981
Size: 1255 690 24 546 786 17 153 185
Cuts at: 3981 4426 325
Size: 445 1498
Fragments arranged by size:
1498 1255 786 690 546 445 185 153
24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2754 2892 3845
Size: 274 80 33 51 1443 777 138 953
Cuts at: 3845 5557 96
Size: 1712 138
Fragments arranged by size:
1712 1443 953 777 274 138 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5559 5591 5559
Size: 32 5567
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5599
XbaI T'CTAG_A
Cuts at: 2935 2935
Size: 5599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2038
Size: 5599
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5599
XmaI C'CCGG_G
Cuts at: 1870 2924 1870
Size: 1054 4545
XmnI GAAnn'nnTTC
Cuts at: 3728 3728
Size: 5599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3407 276
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI