Restriction map for
pFN21AE4296
(Circular) (Six-base) MAPSORT of: pFN21AE4296.seq Check: 6605 from: 1 to: 14125
pFN21AE4296 14125 bp
With 182 enzymes: SgfI *
August 14, 2013 14:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5439 10244 10332 10778 11242 11429 5439
Size: 4805 88 446 464 187 8135
Fragments arranged by size:
8135 4805 464 446 187 88
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4986 11935 278
Size: 53 83 186 936 3450 6949 2468
Fragments arranged by size:
6949 3450 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6191 6945 8022 11468 1937
Size: 4254 754 1077 3446 4594
Fragments arranged by size:
4594 4254 3446 1077 754
Acc65I G'GTAC_C
Cuts at: 3752 11446 3752
Size: 7694 6431
AclI AA'CG_TT
Cuts at: 1563 12252 12625 1563
Size: 10689 373 3063
Fragments arranged by size:
10689 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2795 3133 3426 3708 3832 4174 4463 4612
Size: 1163 338 293 282 124 342 289 149
Cuts at: 4612 5352 5456 5750 7083 7298 7904 7928 8111
Size: 740 104 294 1333 215 606 24 183
Cuts at: 8111 8403 10179 10797 12188 13572 13840 1632
Size: 292 1776 618 1391 1384 268 1917
Fragments arranged by size:
1917 1776 1391 1384 1333 1163 740 618
606 342 338 294 293 292 289 282
268 215 183 149 124 104 24
AflII C'TTAA_G
Cuts at: 820 1017 8443 820
Size: 197 7426 6502
Fragments arranged by size:
7426 6502 197
AflIII A'CryG_T
Cuts at: 1203 4771 6317 8259 9224 9226 11221 13024 14001
Size: 3568 1546 1942 965 2 1995 1803 977
Cuts at: 14001 1203
Size: 1327
Fragments arranged by size:
3568 1995 1942 1803 1546 1327 977 965
2
AgeI A'CCGG_T
Cuts at: 12956 12956
Size: 14125
AhdI GACnn_n'nnGTC
Cuts at: 2416 10843 12854 2416
Size: 8427 2011 3687
Fragments arranged by size:
8427 3687 2011
AleI CACnn'nnGTG
Cuts at: 1166 9227 9241 11042 1166
Size: 8061 14 1801 4249
Fragments arranged by size:
8061 4249 1801 14
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4442 8565 10340 1184
Size: 3258 4123 1775 4969
Fragments arranged by size:
4969 4123 3258 1775
AlwNI CAG_nnn'CTG
Cuts at: 2179 2810 5651 7391 7516 8339 8650 8683 10041
Size: 631 2841 1740 125 823 311 33 1358
Cuts at: 10041 10222 11065 11868 13440 2179
Size: 181 843 803 1572 2864
Fragments arranged by size:
2864 2841 1740 1572 1358 843 823 803
631 311 181 125 33
ApaI G_GGCC'C
Cuts at: 5398 8671 11140 5398
Size: 3273 2469 8383
Fragments arranged by size:
8383 3273 2469
ApaLI G'TGCA_C
Cuts at: 7990 8930 10187 10724 12182 13338 7990
Size: 940 1257 537 1458 1156 8777
Fragments arranged by size:
8777 1458 1257 1156 940 537
ApoI r'AATT_y
Cuts at: 1493 2347 2357 3935 6546 7023 7198 8065 8463
Size: 854 10 1578 2611 477 175 867 398
Cuts at: 8463 8750 10547 11434 11660 11812 13921 1493
Size: 287 1797 887 226 152 2109 1697
Fragments arranged by size:
2611 2109 1797 1697 1578 887 867 854
477 398 287 226 175 152 10
AseI AT'TA_AT
Cuts at: 160 1032 7131 12679 160
Size: 872 6099 5548 1606
Fragments arranged by size:
6099 5548 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14125
AvaI C'yCGr_G
Cuts at: 1870 1943 2501 3164 6160 8573 8664 9806 11450
Size: 73 558 663 2996 2413 91 1142 1644
Cuts at: 11450 1870
Size: 4545
Fragments arranged by size:
4545 2996 2413 1644 1142 663 558 91
73
AvrII C'CTAG_G
Cuts at: 5878 8449 5878
Size: 2571 11554
BamHI G'GATC_C
Cuts at: 7935 9730 10044 10932 11455 11822 7935
Size: 1795 314 888 523 367 10238
Fragments arranged by size:
10238 1795 888 523 367 314
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2531 2962 3752 4373 5401
Size: 325 217 627 744 431 790 621 1028
Cuts at: 5401 8531 9764 10225 11446 12901 618
Size: 3130 1233 461 1221 1455 1842
Fragments arranged by size:
3130 1842 1455 1233 1221 1028 790 744
627 621 461 431 325 217
BanII G_rGCy'C
Cuts at: 1391 2508 3127 4681 5398 7095 7465 7693 8142
Size: 1117 619 1554 717 1697 370 228 449
Cuts at: 8142 8671 9461 10345 11140 11319 11444 1391
Size: 529 790 884 795 179 125 4072
Fragments arranged by size:
4072 1697 1554 1117 884 795 790 717
619 529 449 370 228 179 125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4446 4761 5081 5954 6209 6380 6804
Size: 971 2547 315 320 873 255 171 424
Cuts at: 6804 7263 8557 9477 10206 928
Size: 459 1294 920 729 4847
Fragments arranged by size:
4847 2547 1294 971 920 873 729 459
424 320 315 255 171
BbvCI CC'TCA_GC
Cuts at: 2145 2154 2187 2241 4145 4337 2145
Size: 9 33 54 1904 192 11933
Fragments arranged by size:
11933 1904 192 54 33 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8012 8046 8122 8156 8564 8598 9920
Size: 34 6075 34 76 34 408 34 1322
Cuts at: 9920 9954 12316 12350 1903
Size: 34 2362 34 3678
Fragments arranged by size:
6075 3678 2362 1322 408 76 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5876 12017 13227 5876
Size: 6141 1210 6774
Fragments arranged by size:
6774 6141 1210
BclI T'GATC_A
Cuts at: 1548 3970 4545 7473 7779 10086 1548
Size: 2422 575 2928 306 2307 5587
Fragments arranged by size:
5587 2928 2422 2307 575 306
BfrBI ATG'CAT
Cuts at: 4897 4897
Size: 14125
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3003 3842 6617
Size: 107 122 71 631 605 1331 839 2775
Cuts at: 6617 9509 10748 12736 136
Size: 2892 1239 1988 1525
Fragments arranged by size:
2892 2775 1988 1525 1331 1239 839 631
605 122 107 71
BglII A'GATC_T
Cuts at: 7774 14120 7774
Size: 6346 7779
BlpI GC'TnA_GC
Cuts at: 2571 5803 9638 10060 11542 2571
Size: 3232 3835 422 1482 5154
Fragments arranged by size:
5154 3835 3232 1482 422
Bme1580I G_kGCm'C
Cuts at: 1790 2536 2967 4376 5398 5406 5458 7994 8259
Size: 746 431 1409 1022 8 52 2536 265
Cuts at: 8259 8488 8671 8934 9234 9769 10191 10633 10728
Size: 229 183 263 300 535 422 442 95
Cuts at: 10728 11140 12186 13342 1790
Size: 412 1046 1156 2573
Fragments arranged by size:
2573 2536 1409 1156 1046 1022 746 535
442 431 422 412 300 265 263 229
183 95 52 8
BmgBI CAC'GTC
Cuts at: 1318 5461 5473 9960 11260 1318
Size: 4143 12 4487 1300 4183
Fragments arranged by size:
4487 4183 4143 1300 12
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5102 5687 5971
Size: 416 53 83 399 24 3685 585 284
Cuts at: 5971 6190 7048 8080 8311 8700 10936 11020 11110
Size: 219 858 1032 231 389 2236 84 90
Cuts at: 11110 11856 12814 442
Size: 746 958 1753
Fragments arranged by size:
3685 2236 1753 1032 958 858 746 585
416 399 389 284 231 219 90 84
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 8435 1056
Size: 7379 6746
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3376 7709 7979 10687 3376
Size: 4333 270 2708 6814
Fragments arranged by size:
6814 4333 2708 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2337 3149 3294 3441 3583 6025 6164 7641
Size: 1098 812 145 147 142 2442 139 1477
Cuts at: 7641 7785 8208 8623 8807 8893 8963 8985 9302
Size: 144 423 415 184 86 70 22 317
Cuts at: 9302 9948 9958 10011 10319 10388 10664 11267 12785
Size: 646 10 53 308 69 276 603 1518
Cuts at: 12785 1239
Size: 2579
Fragments arranged by size:
2579 2442 1518 1477 1098 812 646 603
423 415 317 308 276 184 147 145
144 142 139 86 70 69 53 22
10
Bpu10I CC'TnA_GC
Cuts at: 2145 2154 2187 2241 3219 4145 4337 8423 9173
Size: 9 33 54 978 926 192 4086 750
Cuts at: 9173 10310 2145
Size: 1137 5960
Fragments arranged by size:
5960 4086 1137 978 926 750 192 54
33 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2824 3607 8789 10046 11366 12250 13115 13413
Size: 794 783 5182 1257 1320 884 865 298
Cuts at: 13413 2030
Size: 2742
Fragments arranged by size:
5182 2742 1320 1257 884 865 794 783
298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2647 2669 4272 8698 8887 10123 12788
Size: 617 1148 22 1603 4426 189 1236 2665
Cuts at: 12788 882
Size: 2219
Fragments arranged by size:
4426 2665 2219 1603 1236 1148 617 189
22
BsaAI yAC'GTr
Cuts at: 493 1204 4357 8260 9227 10483 11157 11916 493
Size: 711 3153 3903 967 1256 674 759 2702
Fragments arranged by size:
3903 3153 2702 1256 967 759 711 674
BsaBI GATnn'nnATC
Cuts at: 2481 6399 7352 14119 2481
Size: 3918 953 6767 2487
Fragments arranged by size:
6767 3918 2487 953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4983 6114 6989 9933
Size: 53 83 186 936 3450 1131 875 2944
Cuts at: 9933 11932 12314 12949 275
Size: 1999 382 635 1451
Fragments arranged by size:
3450 2944 1999 1451 1131 936 875 635
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3119 4105 6940 7374 8281 12558 12956 13230 13377
Size: 986 2835 434 907 4277 398 274 147
Cuts at: 13377 13788 3119
Size: 411 3456
Fragments arranged by size:
4277 3456 2835 986 907 434 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2533 2563 4149 4179 4894
Size: 30 165 30 1130 30 1586 30 715
Cuts at: 4894 4924 5113 5143 5957 5987 8897 8927 9989
Size: 30 189 30 814 30 2910 30 1062
Cuts at: 9989 10019 1178
Size: 30 5284
Fragments arranged by size:
5284 2910 1586 1130 1062 814 715 189
165 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2133 2258 3239 3590 4308 5381 6028 7643
Size: 947 125 981 351 718 1073 647 1615
Cuts at: 7643 8211 8875 9035 9038 9041 9467 9911 9932
Size: 568 664 160 3 3 426 444 21
Cuts at: 9932 9935 9938 10418 10613 11324 11327 1186
Size: 3 3 480 195 711 3 3984
Fragments arranged by size:
3984 1615 1073 981 947 718 711 664
647 568 480 444 426 351 195 160
125 21 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 5347 6002 9562 9817 10328 13328 13824 5347
Size: 655 3560 255 511 3000 496 5648
Fragments arranged by size:
5648 3560 3000 655 511 496 255
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2422 4017 5640 6155 6255 6344
Size: 573 501 185 1595 1623 515 100 89
Cuts at: 6344 7580 8325 8376 8558 8619 8978 9527 9924
Size: 1236 745 51 182 61 359 549 397
Cuts at: 9924 10331 11051 1163
Size: 407 720 4237
Fragments arranged by size:
4237 1623 1595 1236 745 720 573 549
515 501 407 397 359 185 182 100
89 61 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 11145 11323 11575 12336 13364 1540
Size: 275 186 9144 178 252 761 1028 2301
Fragments arranged by size:
9144 2301 1028 761 275 252 186 178
BsiHKAI G_wGCw'C
Cuts at: 2508 2883 3330 4722 5059 5302 6025 7001 7095
Size: 375 447 1392 337 243 723 976 94
Cuts at: 7095 7743 7965 7994 8934 9531 10191 10307 10728
Size: 648 222 29 940 597 660 116 421
Cuts at: 10728 11319 12186 12271 13342 2508
Size: 591 867 85 1071 3291
Fragments arranged by size:
3291 1392 1071 976 940 867 723 660
648 597 591 447 421 375 337 243
222 116 94 85 29
BsmI GAATG_Cn'
Cuts at: 2450 4419 6250 8398 9290 11636 11729 2450
Size: 1969 1831 2148 892 2346 93 4846
Fragments arranged by size:
4846 2346 2148 1969 1831 892 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4895 8192 9966 4895
Size: 3297 1774 9054
Fragments arranged by size:
9054 3297 1774
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2508 2536 2883 2967 3127 3330 4376
Size: 399 718 28 347 84 160 203 1046
Cuts at: 4376 4681 4722 5059 5302 5398 5406 5458 6025
Size: 305 41 337 243 96 8 52 567
Cuts at: 6025 7001 7095 7465 7693 7743 7965 7994 8142
Size: 976 94 370 228 50 222 29 148
Cuts at: 8142 8259 8488 8671 8934 9234 9461 9531 9769
Size: 117 229 183 263 300 227 70 238
Cuts at: 9769 10191 10307 10345 10633 10728 11140 11319 11444
Size: 422 116 38 288 95 412 179 125
Cuts at: 11444 12186 12271 13342 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1046 976 742 718 567 422
412 399 370 347 337 305 300 288
263 243 238 229 228 227 222 203
183 179 160 148 125 117 116 96
95 94 85 84 70 52 50 41
38 29 28 8
BspEI T'CCGG_A
Cuts at: 4105 7374 8281 13788 4105
Size: 3269 907 5507 4442
Fragments arranged by size:
5507 4442 3269 907
BspHI T'CATG_A
Cuts at: 3472 4500 5029 6880 8400 10977 12014 13744 3472
Size: 1028 529 1851 1520 2577 1037 1730 3853
Fragments arranged by size:
3853 2577 1851 1730 1520 1037 1028 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2971 3458 4332 5439 6328 10244 10332 10386
Size: 2127 487 874 1107 889 3916 88 54
Cuts at: 10386 10778 11242 11429 11480 844
Size: 392 464 187 51 3489
Fragments arranged by size:
3916 3489 2127 1107 889 874 487 464
392 187 88 54 51
BsrBI CCG'CTC
Cuts at: 1394 1823 8529 9811 10867 12012 1394
Size: 429 6706 1282 1056 1145 3507
Fragments arranged by size:
6706 3507 1282 1145 1056 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 9167 9543 9684 12620 12794 65
Size: 1183 7919 376 141 2936 174 1396
Fragments arranged by size:
7919 2936 1396 1183 376 174 141
BsrFI r'CCGG_y
Cuts at: 1700 1810 6616 8590 9151 9554 11379 12769 12956
Size: 110 4806 1974 561 403 1825 1390 187
Cuts at: 12956 1700
Size: 2869
Fragments arranged by size:
4806 2869 1974 1825 1390 561 403 187
110
BsrGI T'GTAC_A
Cuts at: 96 4478 7121 10032 10776 96
Size: 4382 2643 2911 744 3445
Fragments arranged by size:
4382 3445 2911 2643 744
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 14125
BssSI C'ACGA_G
Cuts at: 6214 7793 9437 9779 12185 13197 6214
Size: 1579 1644 342 2406 1012 7142
Fragments arranged by size:
7142 2406 1644 1579 1012 342
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3511 5297 10021 794
Size: 2717 1786 4724 4898
Fragments arranged by size:
4898 4724 2717 1786
BstBI TT'CG_AA
Cuts at: 12929 12929
Size: 14125
BstEII G'GTnAC_C
Cuts at: 1184 9258 11413 1184
Size: 8074 2155 3896
Fragments arranged by size:
8074 3896 2155
BstXI CCAn_nnnn'nTGG
Cuts at: 2704 4745 5984 7619 9243 10924 2704
Size: 2041 1239 1635 1624 1681 5905
Fragments arranged by size:
5905 2041 1681 1635 1624 1239
BstYI r'GATC_y
Cuts at: 1972 3529 5242 5761 5874 7774 7935 8802 8818
Size: 1557 1713 519 113 1900 161 867 16
Cuts at: 8818 9037 9300 9730 10044 10103 10206 10932 11455
Size: 219 263 430 314 59 103 726 523
Cuts at: 11455 11822 12207 12224 13676 13762 13774 14120 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1900 1713 1557 1452 867 726 523
519 430 385 367 346 314 263 219
161 113 103 86 59 17 16 12
Bsu36I CC'TnA_GG
Cuts at: 2951 4233 5644 6122 9890 2951
Size: 1282 1411 478 3768 7186
Fragments arranged by size:
7186 3768 1411 1282 478
BtgI C'CryG_G
Cuts at: 513 1065 2004 3494 3633 3861 4221 4854 5406
Size: 552 939 1490 139 228 360 633 552
Cuts at: 5406 9306 10272 10743 11040 11088 11148 11198 513
Size: 3900 966 471 297 48 60 50 3440
Fragments arranged by size:
3900 3440 1490 966 939 633 552 552
471 360 297 228 139 60 50 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2362 487
Size: 683 56 1136 12250
Fragments arranged by size:
12250 1136 683 56
BtsI GCAGTG_nn'
Cuts at: 2410 2826 3509 3901 3931 4299 4879 5097 5614
Size: 416 683 392 30 368 580 218 517
Cuts at: 5614 5843 6785 7331 7417 8342 8961 9066 10389
Size: 229 942 546 86 925 619 105 1323
Cuts at: 10389 10453 10500 11641 12435 12455 2410
Size: 64 47 1141 794 20 4080
Fragments arranged by size:
4080 1323 1141 942 925 794 683 619
580 546 517 416 392 368 229 218
105 86 64 47 30 20
ClaI AT'CG_AT
Cuts at: 1554 4269 4551 1554
Size: 2715 282 11128
Fragments arranged by size:
11128 2715 282
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 8878 8913 9394 9429 529
Size: 35 8314 35 481 35 5225
Fragments arranged by size:
8314 5225 481 35 35 35
DraI TTT'AAA
Cuts at: 4701 6297 11429 4701
Size: 1596 5132 7397
Fragments arranged by size:
7397 5132 1596
DraIII CAC_nnn'GTG
Cuts at: 5156 8929 9862 10459 5156
Size: 3773 933 597 8822
Fragments arranged by size:
8822 3773 933 597
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 13132 809
Size: 507 11816 1802
Fragments arranged by size:
11816 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3235 3534 4851
Size: 54 1425 50 275 18 1405 299 1317
Cuts at: 4851 5196 5274 5375 5838 6582 7029 8473 9501
Size: 345 78 101 463 744 447 1444 1028
Cuts at: 9501 9552 9841 10077 10926 11034 11151 11572 12461
Size: 51 289 236 849 108 117 421 889
Cuts at: 12461 14090 8
Size: 1629 43
Fragments arranged by size:
1629 1444 1425 1405 1317 1028 889 849
744 463 447 421 345 299 289 275
236 117 108 101 78 54 51 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 11572 1537
Size: 275 9760 4090
Fragments arranged by size:
9760 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3152 3862 5171 5426 5865 7620 7678 7706
Size: 1799 710 1309 255 439 1755 58 28
Cuts at: 7706 7923 8236 8474 9909 12055 1353
Size: 217 313 238 1435 2146 3423
Fragments arranged by size:
3423 2146 1799 1755 1435 1309 710 439
313 255 238 217 58 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2815 3533 4043 4427 6155 10292
Size: 1064 138 1226 718 510 384 1728 4137
Cuts at: 10292 12712 13086 13232 387
Size: 2420 374 146 1280
Fragments arranged by size:
4137 2420 1728 1280 1226 1064 718 510
384 374 146 138
EcoICRI GAG'CTC
Cuts at: 2506 7093 11317 2506
Size: 4587 4224 5314
Fragments arranged by size:
5314 4587 4224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2337 2795 3133 3149 3294 3426 3441
Size: 393 705 458 338 16 145 132 15
Cuts at: 3441 3583 3708 3832 4174 4463 4612 5352 5456
Size: 142 125 124 342 289 149 740 104
Cuts at: 5456 5750 6025 6164 7083 7298 7641 7785 7904
Size: 294 275 139 919 215 343 144 119
Cuts at: 7904 7928 8111 8208 8403 8623 8807 8893 8963
Size: 24 183 97 195 220 184 86 70
Cuts at: 8963 8985 9302 9948 9958 10011 10179 10319 10388
Size: 22 317 646 10 53 168 140 69
Cuts at: 10388 10664 10797 11267 12188 12785 13572 13840 1239
Size: 276 133 470 921 597 787 268 1524
Fragments arranged by size:
1524 921 919 787 740 705 646 597
470 458 393 343 342 338 317 294
289 276 275 268 220 215 195 184
183 168 149 145 144 142 140 139
133 132 125 124 119 104 97 86
70 69 53 24 22 16 15 10
EcoNI CCTnn'n_nnAGG
Cuts at: 3872 5435 9893 3872
Size: 1563 4458 8104
Fragments arranged by size:
8104 4458 1563
EcoO109I rG'GnC_Cy
Cuts at: 3651 3701 4135 4317 4344 5395 5648 6103 8668
Size: 50 434 182 27 1051 253 455 2565
Cuts at: 8668 8773 9483 10071 11136 11137 11328 3651
Size: 105 710 588 1065 1 191 6448
Fragments arranged by size:
6448 2565 1065 1051 710 588 455 434
253 191 182 105 50 27 1
EcoRI G'AATT_C
Cuts at: 6546 7023 11434 11812 6546
Size: 477 4411 378 8859
Fragments arranged by size:
8859 4411 477 378
EcoRV GAT'ATC
Cuts at: 3583 7017 3583
Size: 3434 10691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4229 5518 5971 8225 9349 4229
Size: 1289 453 2254 1124 9005
Fragments arranged by size:
9005 2254 1289 1124 453
FspI TGC'GCA
Cuts at: 12631 12631
Size: 14125
HaeII r_GCGC'y
Cuts at: 1678 3615 6031 10651 13272 1678
Size: 1937 2416 4620 2621 2531
Fragments arranged by size:
4620 2621 2531 2416 1937
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2402 2434 2900 2932 3070
Size: 32 135 32 830 32 466 32 138
Cuts at: 3070 3102 3350 3363 3382 3395 3821 3853 4013
Size: 32 248 13 19 13 426 32 160
Cuts at: 4013 4045 4894 4926 5674 5706 5975 6007 8762
Size: 32 849 32 748 32 269 32 2755
Cuts at: 8762 8794 8803 8835 9984 9989 10016 10021 10198
Size: 32 9 32 1149 5 27 5 177
Cuts at: 10198 10230 10673 10705 10829 10856 10861 10888 10988
Size: 32 443 32 124 27 5 27 100
Cuts at: 10988 11020 12766 12798 12840 12872 1373
Size: 32 1746 32 42 32 2626
Fragments arranged by size:
2755 2626 1746 1149 849 830 748 466
443 426 269 248 177 160 138 135
124 100 42 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 27 27 27 19
13 13 9 5 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 7059 7528 8023 9214 9970 9997
Size: 988 281 5121 469 495 1191 756 27
Cuts at: 9997 11469 11715 669
Size: 1472 246 3079
Fragments arranged by size:
5121 3079 1472 1191 988 756 495 469
281 246 27
HindIII A'AGCT_T
Cuts at: 748 2466 5079 6291 9248 748
Size: 1718 2613 1212 2957 5625
Fragments arranged by size:
5625 2957 2613 1718 1212
HpaI GTT'AAC
Cuts at: 11715 11715
Size: 14125
KpnI G_GTAC'C
Cuts at: 3756 11450 3756
Size: 7694 6431
MfeI C'AATT_G
Cuts at: 5939 11724 5939
Size: 5785 8340
MluI A'CGCG_T
Cuts at: 14001 14001
Size: 14125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5566 6021 6732 7922 8303 10318 10458 11031
Size: 4261 455 711 1190 381 2015 140 573
Cuts at: 11031 11145 13239 13423 1305
Size: 114 2094 184 2007
Fragments arranged by size:
4261 2094 2015 2007 1190 711 573 455
381 184 140 114
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3536 5198 5377 5840 6584
Size: 54 1425 343 1704 1662 179 463 744
Cuts at: 6584 7031 8475 10928 11036 11153 10
Size: 447 1444 2453 108 117 2982
Fragments arranged by size:
2982 2453 1704 1662 1444 1425 744 463
447 343 179 117 108 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 4553 5068 5259 6446 9227 9241
Size: 648 1215 2172 515 191 1187 2781 14
Cuts at: 9241 10772 11042 12083 12442 12601 518
Size: 1531 270 1041 359 159 2042
Fragments arranged by size:
2781 2172 2042 1531 1215 1187 1041 648
515 359 270 191 159 14
MspA1I CmG'CkG
Cuts at: 1606 2179 2251 2690 2807 3215 3540 3713 4149
Size: 573 72 439 117 408 325 173 436
Cuts at: 4149 4163 4484 4543 6361 7276 7391 7545 7848
Size: 14 321 59 1818 915 115 154 303
Cuts at: 7848 8136 8242 8809 8860 9566 10041 10247 11541
Size: 288 106 567 51 706 475 206 1294
Cuts at: 11541 12218 13366 13611 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1818 1294 1148 915 706 677 573
567 475 439 436 408 325 321 303
288 245 206 173 154 117 115 106
72 59 51 14
NaeI GCC'GGC
Cuts at: 9153 9556 9153
Size: 403 13722
NcoI C'CATG_G
Cuts at: 513 1065 2004 3633 3861 5406 10743 11088 11148
Size: 552 939 1629 228 1545 5337 345 60
Cuts at: 11148 513
Size: 3490
Fragments arranged by size:
5337 3490 1629 1545 939 552 345 228
60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 14125
NgoMIV G'CCGG_C
Cuts at: 9151 9554 9151
Size: 403 13722
NheI G'CTAG_C
Cuts at: 1052 8431 1052
Size: 7379 6746
NotI GC'GGCC_GC
Cuts at: 11572 11572
Size: 14125
NsiI A_TGCA'T
Cuts at: 4899 4899
Size: 14125
NspI r_CATG'y
Cuts at: 1132 1229 4775 6321 6611 6952 10777 11225 11483
Size: 97 3546 1546 290 341 3825 448 258
Cuts at: 11483 13028 1132
Size: 1545 2229
Fragments arranged by size:
3825 3546 2229 1546 1545 448 341 290
258 97
PasI CC'CwG_GG
Cuts at: 1348 4761 5383 1348
Size: 3413 622 10090
Fragments arranged by size:
10090 3413 622
PciI A'CATG_T
Cuts at: 4771 6317 11221 13024 4771
Size: 1546 4904 1803 5872
Fragments arranged by size:
5872 4904 1803 1546
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3769 5464 8242 11032 11199 1108
Size: 2661 1695 2778 2790 167 4034
Fragments arranged by size:
4034 2790 2778 2661 1695 167
PfoI T'CCnGG_A
Cuts at: 2670 5463 5479 8115 8940 9318 9974 11094 2670
Size: 2793 16 2636 825 378 656 1120 5701
Fragments arranged by size:
5701 2793 2636 1120 825 656 378 16
PmeI GTTT'AAAC
Cuts at: 11429 11429
Size: 14125
PmlI CAC'GTG
Cuts at: 4357 8260 9227 11157 4357
Size: 3903 967 1930 7325
Fragments arranged by size:
7325 3903 1930 967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4558 4590 4704 4736 6143 6175 12194 12226 13707
Size: 32 114 32 1407 32 6019 32 1481
Cuts at: 13707 13739 4558
Size: 32 4944
Fragments arranged by size:
6019 4944 1481 1407 114 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 3651 4317 4344 5648 9483 10071 3651
Size: 666 27 1304 3835 588 7705
Fragments arranged by size:
7705 3835 1304 666 588 27
PshAI GACnn'nnGTC
Cuts at: 1613 3188 1613
Size: 1575 12550
PsiI TTA'TAA
Cuts at: 4818 6287 11695 4818
Size: 1469 5408 7248
Fragments arranged by size:
7248 5408 1469
PspOMI G'GGCC_C
Cuts at: 5394 8667 11136 5394
Size: 3273 2469 8383
Fragments arranged by size:
8383 3273 2469
PspXI vC'TCGA_Gb
Cuts at: 2501 2501
Size: 14125
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3066 3098 3066
Size: 32 14093
PstI C_TGCA'G
Cuts at: 830 2689 2976 3052 3516 3780 3908 4514 4542
Size: 1859 287 76 464 264 128 606 28
Cuts at: 4542 5850 6627 6777 8587 8859 9073 9111 9798
Size: 1308 777 150 1810 272 214 38 687
Cuts at: 9798 10831 11477 830
Size: 1033 646 3478
Fragments arranged by size:
3478 1859 1810 1308 1033 777 687 646
606 464 287 272 264 214 150 128
76 38 28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 14125
PvuII CAG'CTG
Cuts at: 2690 3540 3713 4149 4163 4484 4543 6361 7276
Size: 850 173 436 14 321 59 1818 915
Cuts at: 7276 7391 8860 9566 10247 2690
Size: 115 1469 706 681 6568
Fragments arranged by size:
6568 1818 1469 915 850 706 681 436
321 173 115 59 14
SacI G_AGCT'C
Cuts at: 2508 7095 11319 2508
Size: 4587 4224 5314
Fragments arranged by size:
5314 4587 4224
SalI G'TCGA_C
Cuts at: 1936 8021 11467 1936
Size: 6085 3446 4594
Fragments arranged by size:
6085 4594 3446
SanDI GG'GwC_CC
Cuts at: 3651 4317 3651
Size: 666 13459
SapI GCTCTTCn'nnn_
Cuts at: 3152 5171 5426 3152
Size: 2019 255 11851
Fragments arranged by size:
11851 2019 255
SbfI CC_TGCA'GG
Cuts at: 11477 11477
Size: 14125
ScaI AGT'ACT
Cuts at: 7156 12373 14085 7156
Size: 5217 1712 7196
Fragments arranged by size:
7196 5217 1712
SexAI A'CCwGG_T
Cuts at: 2583 2613 8327 8682 9620 10221 2583
Size: 30 5714 355 938 601 6487
Fragments arranged by size:
6487 5714 938 601 355 30
SfcI C'TryA_G
Cuts at: 826 1045 2685 2860 2972 3048 3512 3776 3904
Size: 219 1640 175 112 76 464 264 128
Cuts at: 3904 4510 4522 4538 5846 6623 6773 8583 8855
Size: 606 12 16 1308 777 150 1810 272
Cuts at: 8855 9069 9107 9759 9794 10827 11293 11473 12608
Size: 214 38 652 35 1033 466 180 1135
Cuts at: 12608 13289 13480 826
Size: 681 191 1471
Fragments arranged by size:
1810 1640 1471 1308 1135 1033 777 681
652 606 466 464 272 264 219 214
191 180 175 150 128 112 76 38
35 16 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 9509 9509
Size: 14125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14125
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 14125
SmaI CCC'GGG
Cuts at: 1872 8666 11452 1872
Size: 6794 2786 4545
Fragments arranged by size:
6794 4545 2786
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 2501 2839 3586 8443 8768
Size: 197 926 102 456 338 747 4857 325
Cuts at: 8768 10025 11345 12229 13130 13392 820
Size: 1257 1320 884 901 262 1553
Fragments arranged by size:
4857 1553 1320 1257 926 901 884 747
456 338 325 262 197 102
SnaBI TAC'GTA
Cuts at: 493 11916 493
Size: 11423 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 14125
SphI G_CATG'C
Cuts at: 1132 11483 1132
Size: 10351 3774
SrfI GCCC'GGGC
Cuts at: 8666 8666
Size: 14125
SspI AAT'ATT
Cuts at: 5 52 2855 9700 12049 5
Size: 47 2803 6845 2349 2081
Fragments arranged by size:
6845 2803 2349 2081 47
StuI AGG'CCT
Cuts at: 1517 2033 2608 2759 3659 3845 10641 1517
Size: 516 575 151 900 186 6796 5001
Fragments arranged by size:
6796 5001 900 575 516 186 151
StyI C'CwwG_G
Cuts at: 513 1065 2004 2489 2679 3348 3633 3861 4828
Size: 552 939 485 190 669 285 228 967
Cuts at: 4828 5406 5808 5878 6031 6889 8449 9123 9412
Size: 578 402 70 153 858 1560 674 289
Cuts at: 9412 10255 10743 11088 11148 11564 513
Size: 843 488 345 60 416 3074
Fragments arranged by size:
3074 1560 967 939 858 843 674 669
578 552 488 485 416 402 345 289
285 228 190 153 70 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6104 6113 7496 9277 9722 9970 12152
Size: 1255 4524 9 1383 1781 445 248 2182
Cuts at: 12152 12169 12322 12507 12952 325
Size: 17 153 185 445 1498
Fragments arranged by size:
4524 2182 1781 1498 1383 1255 445 445
248 185 153 17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4478 6303 7121
Size: 274 80 33 51 1443 2501 1825 818
Cuts at: 7121 7154 7804 10032 10776 12371 14083 96
Size: 33 650 2228 744 1595 1712 138
Fragments arranged by size:
2501 2228 1825 1712 1595 1443 818 744
650 274 138 80 51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2533 2565 2805 2837 4026 4058 4080 4112 4149
Size: 32 240 32 1189 32 22 32 37
Cuts at: 4149 4181 7571 7603 8897 8929 9037 9069 14085
Size: 32 3390 32 1294 32 108 32 5016
Cuts at: 14085 14117 2533
Size: 32 2541
Fragments arranged by size:
5016 3390 2541 1294 1189 240 108 37
32 32 32 32 32 32 32 32
32 22
Tth111I GACn'n_nGTC
Cuts at: 1316 7581 8329 11102 1316
Size: 6265 748 2773 4339
Fragments arranged by size:
6265 4339 2773 748
XbaI T'CTAG_A
Cuts at: 5627 10948 11461 5627
Size: 5321 513 8291
Fragments arranged by size:
8291 5321 513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 5115 9520 10750 2011
Size: 3104 4405 1230 5386
Fragments arranged by size:
5386 4405 3104 1230
XhoI C'TCGA_G
Cuts at: 1943 2501 1943
Size: 558 13567
XmaI C'CCGG_G
Cuts at: 1870 8664 11450 1870
Size: 6794 2786 4545
Fragments arranged by size:
6794 4545 2786
XmnI GAAnn'nnTTC
Cuts at: 2033 2391 8052 8124 8396 11267 12254 2033
Size: 358 5661 72 272 2871 987 3904
Fragments arranged by size:
5661 3904 2871 987 358 272 72
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4984 11933 276
Size: 53 83 186 936 3450 6949 2468
Fragments arranged by size:
6949 3450 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BsiWI BstZ17I FseI
FspAI KasI NarI NruI PacI RsrII SacII SfoI
SwaI