Restriction map for
pFN21AE4279
(Circular) (Six-base) MAPSORT of: pFN21AE4279.seq Check: 4696 from: 1 to: 8851
pFN21AE4279 8851 bp
With 182 enzymes: SgfI *
March 28, 2012 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4229 4229
Size: 8851
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5235 6661 278
Size: 53 83 186 936 3699 1426 2468
Fragments arranged by size:
3699 2468 1426 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6194 1937
Size: 4257 4594
Acc65I G'GTAC_C
Cuts at: 4047 6172 4047
Size: 2125 6726
AclI AA'CG_TT
Cuts at: 1563 6978 7351 1563
Size: 5415 373 3063
Fragments arranged by size:
5415 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2696 5589 6914 8298 8566 1632
Size: 1064 2893 1325 1384 268 1917
Fragments arranged by size:
2893 1917 1384 1325 1064 268
AfeI AGC'GCT
Cuts at: 2531 2531
Size: 8851
AflII C'TTAA_G
Cuts at: 820 1017 2824 820
Size: 197 1807 6847
Fragments arranged by size:
6847 1807 197
AflIII A'CryG_T
Cuts at: 1203 3847 7750 8727 1203
Size: 2644 3903 977 1327
Fragments arranged by size:
3903 2644 1327 977
AgeI A'CCGG_T
Cuts at: 3040 5704 7682 3040
Size: 2664 1978 4209
Fragments arranged by size:
4209 2664 1978
AhdI GACnn_n'nnGTC
Cuts at: 7580 7580
Size: 8851
AleI CACnn'nnGTG
Cuts at: 1166 4610 4721 5091 1166
Size: 3444 111 370 4926
Fragments arranged by size:
4926 3444 370 111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5268 1184
Size: 4084 4767
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5004 5036 5004
Size: 32 8819
AlwNI CAG_nnn'CTG
Cuts at: 3311 3442 6594 8166 3311
Size: 131 3152 1572 3996
Fragments arranged by size:
3996 3152 1572 131
ApaI G_GGCC'C
Cuts at: 3142 3142
Size: 8851
ApaLI G'TGCA_C
Cuts at: 5165 6908 8064 5165
Size: 1743 1156 5952
Fragments arranged by size:
5952 1743 1156
ApoI r'AATT_y
Cuts at: 1493 2744 2833 3904 3958 4448 4924 5033 5507
Size: 1251 89 1071 54 490 476 109 474
Cuts at: 5507 6160 6386 6538 8647 1493
Size: 653 226 152 2109 1697
Fragments arranged by size:
2109 1697 1251 1071 653 490 476 474
226 152 109 89 54
AscI GG'CGCG_CC
Cuts at: 2085 2085
Size: 8851
AseI AT'TA_AT
Cuts at: 160 1032 7405 160
Size: 872 6373 1606
Fragments arranged by size:
6373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8851
AvaI C'yCGr_G
Cuts at: 1870 1943 5801 6176 1870
Size: 73 3858 375 4545
Fragments arranged by size:
4545 3858 375 73
BamHI G'GATC_C
Cuts at: 6181 6548 6181
Size: 367 8484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4047 4819 4861 6146 6172
Size: 325 217 627 2260 772 42 1285 26
Cuts at: 6172 7627 618
Size: 1455 1842
Fragments arranged by size:
2260 1842 1455 1285 772 627 325 217
42 26
BanII G_rGCy'C
Cuts at: 1391 2240 3142 3649 4903 5262 6170 1391
Size: 849 902 507 1254 359 908 4072
Fragments arranged by size:
4072 1254 908 902 849 507 359
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4142 5146 5577 928
Size: 971 2243 1004 431 4202
Fragments arranged by size:
4202 2243 1004 971 431
BbvCI CC'TCA_GC
Cuts at: 4932 5687 4932
Size: 755 8096
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3631 3665 5680 5714 7042
Size: 34 50 34 1610 34 2015 34 1328
Cuts at: 7042 7076 1903
Size: 34 3678
Fragments arranged by size:
3678 2015 1610 1328 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4742 6743 7953 4742
Size: 2001 1210 5640
Fragments arranged by size:
5640 2001 1210
BclI T'GATC_A
Cuts at: 1548 3562 5725 1548
Size: 2014 2163 4674
Fragments arranged by size:
4674 2163 2014
BfrBI ATG'CAT
Cuts at: 3208 4162 3208
Size: 954 7897
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 2201 3925
Size: 107 122 71 631 605 430 99 1724
Cuts at: 3925 7462 136
Size: 3537 1525
Fragments arranged by size:
3537 1724 1525 631 605 430 122 107
99 71
BglII A'GATC_T
Cuts at: 4693 4972 8846 4693
Size: 279 3874 4698
Fragments arranged by size:
4698 3874 279
BlpI GC'TnA_GC
Cuts at: 6268 6268
Size: 8851
Bme1580I G_kGCm'C
Cuts at: 1790 3142 4824 5169 5355 6912 8068 1790
Size: 1352 1682 345 186 1557 1156 2573
Fragments arranged by size:
2573 1682 1557 1352 1156 345 186
BmgBI CAC'GTC
Cuts at: 1318 2140 2364 1318
Size: 822 224 7805
Fragments arranged by size:
7805 822 224
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3136 5432 6582
Size: 416 53 83 399 24 1719 2296 1150
Cuts at: 6582 7540 442
Size: 958 1753
Fragments arranged by size:
2296 1753 1719 1150 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8851
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5252 5252
Size: 8851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2721 3053 5110 5769 7511 1239
Size: 1482 332 2057 659 1742 2579
Fragments arranged by size:
2579 2057 1742 1482 659 332
Bpu10I CC'TnA_GC
Cuts at: 4598 4932 5687 4598
Size: 334 755 7762
Fragments arranged by size:
7762 755 334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4887 5288 6976 7841 8139 4887
Size: 401 1688 865 298 5599
Fragments arranged by size:
5599 1688 865 401 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5047 7514 882
Size: 617 3548 2467 2219
Fragments arranged by size:
3548 2467 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5182 6642 493
Size: 711 3978 1460 2702
Fragments arranged by size:
3978 2702 1460 711
BsaBI GATnn'nnATC
Cuts at: 4977 8845 4977
Size: 3868 4983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5232 5562 6658 7040
Size: 53 83 186 936 3699 330 1096 382
Cuts at: 7040 7675 275
Size: 635 1451
Fragments arranged by size:
3699 1451 1096 936 635 382 330 186
83 53
BsaWI w'CCGG_w
Cuts at: 3040 5388 5704 7284 7682 7956 8103 8514 3040
Size: 2348 316 1580 398 274 147 411 3377
Fragments arranged by size:
3377 2348 1580 411 398 316 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2619 2649 4031 4061 5807
Size: 30 165 30 1216 30 1382 30 1746
Cuts at: 5807 5837 1178
Size: 30 4192
Fragments arranged by size:
4192 1746 1382 1216 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 3012 4298 5282 5675 1186
Size: 949 877 1286 984 393 4362
Fragments arranged by size:
4362 1286 984 949 877 393
BseYI C'CCAG_C
Cuts at: 2080 2559 8054 8550 2080
Size: 479 5495 496 2381
Fragments arranged by size:
5495 2381 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 5119 5162 1163
Size: 573 411 2972 43 4852
Fragments arranged by size:
4852 2972 573 411 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2201 2205 6085 6301 7062 8090
Size: 275 186 200 4 3880 216 761 1028
Cuts at: 8090 1540
Size: 2301
Fragments arranged by size:
3880 2301 1028 761 275 216 200 186
4
BsiHKAI G_wGCw'C
Cuts at: 2240 3000 3649 3727 4903 5169 5262 5808 6144
Size: 760 649 78 1176 266 93 546 336
Cuts at: 6144 6912 6997 8068 2240
Size: 768 85 1071 3023
Fragments arranged by size:
3023 1176 1071 768 760 649 546 336
266 93 85 78
BsmI GAATG_Cn'
Cuts at: 5592 6362 6455 5592
Size: 770 93 7988
Fragments arranged by size:
7988 770 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4594 5223 4594
Size: 629 8222
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 3000 3142 3649 3727 4824 4903
Size: 399 450 760 142 507 78 1097 79
Cuts at: 4903 5169 5262 5355 5808 6144 6170 6912 6997
Size: 266 93 93 453 336 26 742 85
Cuts at: 6997 8068 1391
Size: 1071 2174
Fragments arranged by size:
2174 1097 1071 760 742 507 453 450
399 336 266 142 93 93 85 79
78 26
BspEI T'CCGG_A
Cuts at: 5388 8514 5388
Size: 3126 5725
BspHI T'CATG_A
Cuts at: 6740 8470 6740
Size: 1730 7121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3235 4229 6206 844
Size: 2391 994 1977 3489
Fragments arranged by size:
3489 2391 1977 994
BsrBI CCG'CTC
Cuts at: 1394 1823 5130 6738 1394
Size: 429 3307 1608 3507
Fragments arranged by size:
3507 3307 1608 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3921 5955 7346 7520 65
Size: 1183 2673 2034 1391 174 1396
Fragments arranged by size:
2673 2034 1396 1391 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2200 3040 3522 5704 7495 7682 1700
Size: 110 390 840 482 2182 1791 187 2869
Fragments arranged by size:
2869 2182 1791 840 482 390 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8851
BssHII G'CGCG_C
Cuts at: 1925 2085 1925
Size: 160 8691
BssSI C'ACGA_G
Cuts at: 6911 7923 6911
Size: 1012 7839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4868 794
Size: 4074 4777
BstBI TT'CG_AA
Cuts at: 2214 7655 2214
Size: 5441 3410
BstEII G'GTnAC_C
Cuts at: 1184 2062 2554 1184
Size: 878 492 7481
Fragments arranged by size:
7481 878 492
BstXI CCAn_nnnn'nTGG
Cuts at: 5422 5422
Size: 8851
BstYI r'GATC_y
Cuts at: 1972 4642 4693 4972 5624 6181 6548 6933 6950
Size: 2670 51 279 652 557 367 385 17
Cuts at: 6950 8402 8488 8500 8846 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2670 1977 1452 652 557 385 367 346
279 86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2160 2451 2464 2472 513
Size: 552 1095 291 13 8 6892
Fragments arranged by size:
6892 1095 552 291 13 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2572 487
Size: 683 56 1346 6766
Fragments arranged by size:
6766 1346 683 56
BtsI GCAGTG_nn'
Cuts at: 2308 2362 3169 3657 3738 6367 7161 7181 2308
Size: 54 807 488 81 2629 794 20 3978
Fragments arranged by size:
3978 2629 807 794 488 81 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3325 3360 529
Size: 35 2761 35 6020
Fragments arranged by size:
6020 2761 35 35
DraI TTT'AAA
Cuts at: 4009 4477 6155 4009
Size: 468 1678 6705
Fragments arranged by size:
6705 1678 468
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4401 7858 809
Size: 507 3085 3457 1802
Fragments arranged by size:
3457 3085 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2198 2202
Size: 54 1425 50 275 18 282 86 4
Cuts at: 2202 3654 6298 7187 8816 8
Size: 1452 2644 889 1629 43
Fragments arranged by size:
2644 1629 1452 1425 889 282 275 86
54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2198 2202 6298 1537
Size: 275 386 4 4096 4090
Fragments arranged by size:
4096 4090 386 275 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2415 2967 3784 6781 1353
Size: 1062 552 817 2997 3423
Fragments arranged by size:
3423 2997 1062 817 552
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2524 7438 7812 7958 387
Size: 1064 138 935 4914 374 146 1280
Fragments arranged by size:
4914 1280 1064 935 374 146 138
EcoICRI GAG'CTC
Cuts at: 2238 3647 4901 5260 2238
Size: 1409 1254 359 5829
Fragments arranged by size:
5829 1409 1254 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2696 2721 3053 5110 5589 5769 6914
Size: 393 1064 25 332 2057 479 180 1145
Cuts at: 6914 7511 8298 8566 1239
Size: 597 787 268 1524
Fragments arranged by size:
2057 1524 1145 1064 787 597 479 393
332 268 180 25
EcoNI CCTnn'n_nnAGG
Cuts at: 5147 5147
Size: 8851
EcoO109I rG'GnC_Cy
Cuts at: 3138 4315 4677 5308 3138
Size: 1177 362 631 6681
Fragments arranged by size:
6681 1177 631 362
EcoRI G'AATT_C
Cuts at: 2744 4448 6160 6538 2744
Size: 1704 1712 378 5057
Fragments arranged by size:
5057 1712 1704 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2355 3288 4547 5329 6127 2355
Size: 933 1259 782 798 5079
Fragments arranged by size:
5079 1259 933 798 782
FseI GG_CCGG'CC
Cuts at: 2204 2204
Size: 8851
FspI TGC'GCA
Cuts at: 5943 7357 5943
Size: 1414 7437
HaeII r_GCGC'y
Cuts at: 1678 2134 2154 2533 7998 1678
Size: 456 20 379 5465 2531
Fragments arranged by size:
5465 2531 456 379 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2414 2446 4627 4659 4890
Size: 32 135 32 842 32 2181 32 231
Cuts at: 4890 4922 4984 5016 7492 7524 7566 7598 1373
Size: 32 62 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 2181 842 231 135 62 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6195 6441 669
Size: 988 281 4257 246 3079
Fragments arranged by size:
4257 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4787 5969 748
Size: 4039 1182 3630
Fragments arranged by size:
4039 3630 1182
HpaI GTT'AAC
Cuts at: 6441 6441
Size: 8851
KpnI G_GTAC'C
Cuts at: 4051 6176 4051
Size: 2125 6726
MfeI C'AATT_G
Cuts at: 6450 6450
Size: 8851
MluI A'CGCG_T
Cuts at: 8727 8727
Size: 8851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2332 3132 3700 4487 7965 8149 1305
Size: 1027 800 568 787 3478 184 2007
Fragments arranged by size:
3478 2007 1027 800 787 568 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3656 10
Size: 54 1425 343 1824 5205
Fragments arranged by size:
5205 1824 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3086 3264 3768 3936 4610 4721 5091
Size: 648 1920 178 504 168 674 111 370
Cuts at: 5091 5184 6809 7168 7327 518
Size: 93 1625 359 159 2042
Fragments arranged by size:
2042 1920 1625 674 648 504 370 359
178 168 159 111 93
MspA1I CmG'CkG
Cuts at: 1606 2162 2207 2877 3471 6267 6944 8092 8337
Size: 556 45 670 594 2796 677 1148 245
Cuts at: 8337 1606
Size: 2120
Fragments arranged by size:
2796 2120 1148 677 670 594 556 245
45
NaeI GCC'GGC
Cuts at: 2202 2202
Size: 8851
NcoI C'CATG_G
Cuts at: 513 1065 2464 513
Size: 552 1399 6900
Fragments arranged by size:
6900 1399 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8851
NgoMIV G'CCGG_C
Cuts at: 2200 2200
Size: 8851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8851
NotI GC'GGCC_GC
Cuts at: 6298 6298
Size: 8851
NsiI A_TGCA'T
Cuts at: 3210 4164 3210
Size: 954 7897
NspI r_CATG'y
Cuts at: 1132 1229 3851 4162 4166 5695 6209 7754 1132
Size: 97 2622 311 4 1529 514 1545 2229
Fragments arranged by size:
2622 2229 1545 1529 514 311 97 4
PasI CC'CwG_GG
Cuts at: 1348 2372 2623 3311 1348
Size: 1024 251 688 6888
Fragments arranged by size:
6888 1024 688 251
PciI A'CATG_T
Cuts at: 3847 7750 3847
Size: 3903 4948
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2140 2318 3311 5029 5109 6133 1108
Size: 1032 178 993 1718 80 1024 3826
Fragments arranged by size:
3826 1718 1032 1024 993 178 80
PfoI T'CCnGG_A
Cuts at: 3030 5208 3030
Size: 2178 6673
PmeI GTTT'AAAC
Cuts at: 6155 6155
Size: 8851
PmlI CAC'GTG
Cuts at: 5182 5182
Size: 8851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6920 6952 8433 8465 6920
Size: 32 1481 32 7306
Fragments arranged by size:
7306 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4315 4677 4315
Size: 362 8489
PshAI GACnn'nnGTC
Cuts at: 1613 3502 4328 1613
Size: 1889 826 6136
Fragments arranged by size:
6136 1889 826
PsiI TTA'TAA
Cuts at: 2385 6421 2385
Size: 4036 4815
PspOMI G'GGCC_C
Cuts at: 3138 3138
Size: 8851
PstI C_TGCA'G
Cuts at: 830 2546 4492 4555 6203 830
Size: 1716 1946 63 1648 3478
Fragments arranged by size:
3478 1946 1716 1648 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8851
PvuII CAG'CTG
Cuts at: 2877 3471 2877
Size: 594 8257
SacI G_AGCT'C
Cuts at: 2240 3649 4903 5262 2240
Size: 1409 1254 359 5829
Fragments arranged by size:
5829 1409 1254 359
SacII CC_GC'GG
Cuts at: 2163 2163
Size: 8851
SalI G'TCGA_C
Cuts at: 1936 6193 1936
Size: 4257 4594
SbfI CC_TGCA'GG
Cuts at: 6203 6203
Size: 8851
ScaI AGT'ACT
Cuts at: 5198 5741 7099 8811 5198
Size: 543 1358 1712 5238
Fragments arranged by size:
5238 1712 1358 543
SexAI A'CCwGG_T
Cuts at: 2643 2643
Size: 8851
SfcI C'TryA_G
Cuts at: 826 1045 2542 4223 4379 4488 4551 5781 6199
Size: 219 1497 1681 156 109 63 1230 418
Cuts at: 6199 7334 8015 8206 826
Size: 1135 681 191 1471
Fragments arranged by size:
1681 1497 1471 1230 1135 681 418 219
191 156 109 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 2201 2201
Size: 8851
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8851
SgrAI Cr'CCGG_yG
Cuts at: 1700 5704 1700
Size: 4004 4847
SmaI CCC'GGG
Cuts at: 1872 6178 1872
Size: 4306 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2824 4902 5303 6955 7856 8118
Size: 197 926 881 2078 401 1652 901 262
Cuts at: 8118 820
Size: 1553
Fragments arranged by size:
2078 1652 1553 926 901 881 401 262
197
SnaBI TAC'GTA
Cuts at: 493 6642 493
Size: 6149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8851
SphI G_CATG'C
Cuts at: 1132 6209 1132
Size: 5077 3774
SspI AAT'ATT
Cuts at: 5 52 3343 5866 6775 5
Size: 47 3291 2523 909 2081
Fragments arranged by size:
3291 2523 2081 909 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8851
StyI C'CwwG_G
Cuts at: 513 1065 2330 2464 3841 5999 6290 513
Size: 552 1265 134 1377 2158 291 3074
Fragments arranged by size:
3074 2158 1377 1265 552 291 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2573 3179 3623 4851 6878 6895 7048
Size: 1255 993 606 444 1228 2027 17 153
Cuts at: 7048 7233 7678 325
Size: 185 445 1498
Fragments arranged by size:
2027 1498 1255 1228 993 606 445 444
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2770 5196 5739
Size: 274 80 33 51 1443 793 2426 543
Cuts at: 5739 7097 8809 96
Size: 1358 1712 138
Fragments arranged by size:
2426 1712 1443 1358 793 543 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2619 2651 8811 8843 2619
Size: 32 6160 32 2627
Fragments arranged by size:
6160 2627 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4850 1316
Size: 3534 5317
XbaI T'CTAG_A
Cuts at: 3506 3862 6187 3506
Size: 356 2325 6170
Fragments arranged by size:
6170 2325 356
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2315 2737 2315
Size: 422 8429
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8851
XmaI C'CCGG_G
Cuts at: 1870 6176 1870
Size: 4306 4545
XmnI GAAnn'nnTTC
Cuts at: 2833 5153 5574 6980 2833
Size: 2320 421 1406 4704
Fragments arranged by size:
4704 2320 1406 421
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5233 6659 276
Size: 53 83 186 936 3699 1426 2468
Fragments arranged by size:
3699 2468 1426 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoRV
FspAI KasI NarI NruI PacI PspXI PsrI RsrII
SanDI SapI SfoI SrfI SwaI