Restriction map for
pFN21AE4271
(Circular) (Six-base) MAPSORT of: pFN21AE4271.seq Check: 5632 from: 1 to: 8116
pFN21AE4271 8116 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5926 278
Size: 53 83 186 936 4390 2468
Fragments arranged by size:
4390 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2093 2857 4294 5459 1937
Size: 156 764 1437 1165 4594
Fragments arranged by size:
4594 1437 1165 764 156
Acc65I G'GTAC_C
Cuts at: 5437 5437
Size: 8116
AclI AA'CG_TT
Cuts at: 1563 6243 6616 1563
Size: 4680 373 3063
Fragments arranged by size:
4680 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2574 6179 7563 7831 1632
Size: 942 3605 1384 268 1917
Fragments arranged by size:
3605 1917 1384 942 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7919
AflIII A'CryG_T
Cuts at: 1203 7015 7992 1203
Size: 5812 977 1327
Fragments arranged by size:
5812 1327 977
AgeI A'CCGG_T
Cuts at: 6947 6947
Size: 8116
AhdI GACnn_n'nnGTC
Cuts at: 6845 6845
Size: 8116
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2754 1184
Size: 1570 6546
AlwNI CAG_nnn'CTG
Cuts at: 2057 2165 5859 7431 2057
Size: 108 3694 1572 2742
Fragments arranged by size:
3694 2742 1572 108
ApaLI G'TGCA_C
Cuts at: 6173 7329 6173
Size: 1156 6960
ApoI r'AATT_y
Cuts at: 1493 2420 2638 3360 3539 3574 3602 3956 4026
Size: 927 218 722 179 35 28 354 70
Cuts at: 4026 4158 4442 5247 5425 5651 5803 7912 1493
Size: 132 284 805 178 226 152 2109 1697
Fragments arranged by size:
2109 1697 927 805 722 354 284 226
218 179 178 152 132 70 35 28
AseI AT'TA_AT
Cuts at: 160 1032 2574 3615 5291 6670 160
Size: 872 1542 1041 1676 1379 1606
Fragments arranged by size:
1676 1606 1542 1379 1041 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8116
AvaI C'yCGr_G
Cuts at: 1870 1943 2805 5441 1870
Size: 73 862 2636 4545
Fragments arranged by size:
4545 2636 862 73
BamHI G'GATC_C
Cuts at: 5128 5446 5813 5128
Size: 318 367 7431
Fragments arranged by size:
7431 367 318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4593 5437 6892 618
Size: 325 217 627 2806 844 1455 1842
Fragments arranged by size:
2806 1842 1455 844 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2814 4715 5435 1391
Size: 1423 1901 720 4072
Fragments arranged by size:
4072 1901 1423 720
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2516 928
Size: 971 617 6528
Fragments arranged by size:
6528 971 617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6307 6341 1903
Size: 34 4370 34 3678
Fragments arranged by size:
4370 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6008 7218 6008
Size: 1210 6906
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8116
BfrBI ATG'CAT
Cuts at: 4189 5344 4189
Size: 1155 6961
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2180 6727 136
Size: 107 122 71 631 605 508 4547 1525
Fragments arranged by size:
4547 1525 631 605 508 122 107 71
BglII A'GATC_T
Cuts at: 8111 8111
Size: 8116
BlpI GC'TnA_GC
Cuts at: 2050 4527 5533 2050
Size: 2477 1006 4633
Fragments arranged by size:
4633 2477 1006
Bme1580I G_kGCm'C
Cuts at: 1790 6177 7333 1790
Size: 4387 1156 2573
Fragments arranged by size:
4387 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8116
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5847 6805 442
Size: 416 53 83 399 24 4430 958 1753
Fragments arranged by size:
4430 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2029 5262 6776 1239
Size: 790 3233 1514 2579
Fragments arranged by size:
3233 2579 1514 790
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5006 5300 6241 7106 7404 5006
Size: 294 941 865 298 5718
Fragments arranged by size:
5718 941 865 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2263 6779 882
Size: 617 764 4516 2219
Fragments arranged by size:
4516 2219 764 617
BsaAI yAC'GTr
Cuts at: 493 1204 2239 3851 5907 493
Size: 711 1035 1612 2056 2702
Fragments arranged by size:
2702 2056 1612 1035 711
BsaBI GATnn'nnATC
Cuts at: 3036 4742 8110 3036
Size: 1706 3368 3042
Fragments arranged by size:
3368 3042 1706
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5923 6305 6940 275
Size: 53 83 186 936 4390 382 635 1451
Fragments arranged by size:
4390 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4008 4688 6549 6947 7221 7368 7779 4008
Size: 680 1861 398 274 147 411 4345
Fragments arranged by size:
4345 1861 680 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3018 3048 1178
Size: 30 165 30 1615 30 6246
Fragments arranged by size:
6246 1615 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2107 2110 4028 5390 1186
Size: 921 3 1918 1362 3912
Fragments arranged by size:
3912 1918 1362 921 3
BseYI C'CCAG_C
Cuts at: 2194 2203 2223 3747 4615 7319 7815 2194
Size: 9 20 1524 868 2704 496 2495
Fragments arranged by size:
2704 2495 1524 868 496 20 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2151 3203 3368 4212 1163
Size: 573 415 1052 165 844 5067
Fragments arranged by size:
5067 1052 844 573 415 165
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2175 2184 5566 6327 7355 1540
Size: 275 186 174 9 3382 761 1028 2301
Fragments arranged by size:
3382 2301 1028 761 275 186 174 9
BsiHKAI G_wGCw'C
Cuts at: 2814 4715 4952 6177 6262 7333 2814
Size: 1901 237 1225 85 1071 3597
Fragments arranged by size:
3597 1901 1225 1071 237 85
BsmI GAATG_Cn'
Cuts at: 2545 4191 5627 5720 2545
Size: 1646 1436 93 4941
Fragments arranged by size:
4941 1646 1436 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3715 3715
Size: 8116
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2814 4715 4952 5435 6177 6262 7333
Size: 399 1024 1901 237 483 742 85 1071
Cuts at: 7333 1391
Size: 2174
Fragments arranged by size:
2174 1901 1071 1024 742 483 399 237
85
BspEI T'CCGG_A
Cuts at: 4008 7779 4008
Size: 3771 4345
BspHI T'CATG_A
Cuts at: 2632 4954 6005 7735 2632
Size: 2322 1051 1730 3013
Fragments arranged by size:
3013 2322 1730 1051
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3982 5367 5471 844
Size: 3138 1385 104 3489
Fragments arranged by size:
3489 3138 1385 104
BsrBI CCG'CTC
Cuts at: 1394 1823 6003 1394
Size: 429 4180 3507
Fragments arranged by size:
4180 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4968 6611 6785 65
Size: 1183 3720 1643 174 1396
Fragments arranged by size:
3720 1643 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6760 6947 1700
Size: 110 4950 187 2869
Fragments arranged by size:
4950 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4555 96
Size: 4459 3657
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8116
BssSI C'ACGA_G
Cuts at: 6176 7188 6176
Size: 1012 7104
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2165 2540 2798 794
Size: 1371 375 258 6112
Fragments arranged by size:
6112 1371 375 258
BstBI TT'CG_AA
Cuts at: 2561 6920 2561
Size: 4359 3757
BstEII G'GTnAC_C
Cuts at: 1184 3742 1184
Size: 2558 5558
BstXI CCAn_nnnn'nTGG
Cuts at: 4305 4305
Size: 8116
BstYI r'GATC_y
Cuts at: 1972 4179 5128 5446 5813 6198 6215 7667 7753
Size: 2207 949 318 367 385 17 1452 86
Cuts at: 7753 7765 8111 1972
Size: 12 346 1977
Fragments arranged by size:
2207 1977 1452 949 385 367 346 318
86 17 12
BstZ17I GTA'TAC
Cuts at: 2858 4295 2858
Size: 1437 6679
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7564
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7377
Fragments arranged by size:
7377 683 56
BtsI GCAGTG_nn'
Cuts at: 4420 4482 5632 6426 6446 4420
Size: 62 1150 794 20 6090
Fragments arranged by size:
6090 1150 794 62 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8081
DraI TTT'AAA
Cuts at: 3580 4114 5420 3580
Size: 534 1306 6276
Fragments arranged by size:
6276 1306 534
DraIII CAC_nnn'GTG
Cuts at: 4600 5077 4600
Size: 477 7639
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7123 809
Size: 507 5807 1802
Fragments arranged by size:
5807 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2181 4515
Size: 54 1425 50 275 18 342 9 2334
Cuts at: 4515 5563 6452 8081 8
Size: 1048 889 1629 43
Fragments arranged by size:
2334 1629 1425 1048 889 342 275 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2172 2181 5563 1537
Size: 275 360 9 3382 4090
Fragments arranged by size:
4090 3382 360 275 9
EarI CTCTTCn'nnn_
Cuts at: 1353 2261 4933 5412 6046 1353
Size: 908 2672 479 634 3423
Fragments arranged by size:
3423 2672 908 634 479
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5028 6703 7077 7223 387
Size: 1064 138 3439 1675 374 146 1280
Fragments arranged by size:
3439 1675 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2812 4713 2812
Size: 1901 6215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2574 5262 6179 6776 7563 7831
Size: 393 397 545 2688 917 597 787 268
Cuts at: 7831 1239
Size: 1524
Fragments arranged by size:
2688 1524 917 787 597 545 397 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3192 4517 3192
Size: 1325 6791
EcoRI G'AATT_C
Cuts at: 2420 3360 3539 5425 5803 2420
Size: 940 179 1886 378 4733
Fragments arranged by size:
4733 1886 940 378 179
EcoRV GAT'ATC
Cuts at: 2518 2518
Size: 8116
FspI TGC'GCA
Cuts at: 6622 6622
Size: 8116
HaeII r_GCGC'y
Cuts at: 1678 2157 2192 2201 3320 7263 1678
Size: 479 35 9 1119 3943 2531
Fragments arranged by size:
3943 2531 1119 479 35 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2452 2484 3779 3811 3826
Size: 32 135 32 880 32 1295 32 15
Cuts at: 3826 3858 4936 4968 4994 5026 5088 5120 6757
Size: 32 1078 32 26 32 62 32 1637
Cuts at: 6757 6789 6831 6863 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1637 1295 1078 880 135 62 42
32 32 32 32 32 32 32 32
32 32 26 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3448 3484 4498 5460 5706 669
Size: 988 281 1510 36 1014 962 246 3079
Fragments arranged by size:
3079 1510 1014 988 962 281 246 36
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8116
HpaI GTT'AAC
Cuts at: 3484 5706 3484
Size: 2222 5894
KpnI G_GTAC'C
Cuts at: 5441 5441
Size: 8116
MfeI C'AATT_G
Cuts at: 4788 5715 4788
Size: 927 7189
MluI A'CGCG_T
Cuts at: 7992 7992
Size: 8116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2341 4297 7230 7414 1305
Size: 1036 1956 2933 184 2007
Fragments arranged by size:
2933 2007 1956 1036 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4517 10
Size: 54 1425 343 2685 3609
Fragments arranged by size:
3609 2685 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2717 2736 4817 6074 6433 6592 518
Size: 648 1551 19 2081 1257 359 159 2042
Fragments arranged by size:
2081 2042 1551 1257 648 359 159 19
MspA1I CmG'CkG
Cuts at: 1606 2162 2171 2180 2227 5532 6209 7357 7602
Size: 556 9 9 47 3305 677 1148 245
Cuts at: 7602 1606
Size: 2120
Fragments arranged by size:
3305 2120 1148 677 556 245 47 9
9
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7564
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8116
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8116
NotI GC'GGCC_GC
Cuts at: 2172 2181 5563 2172
Size: 9 3382 4725
Fragments arranged by size:
4725 3382 9
NsiI A_TGCA'T
Cuts at: 4191 5346 4191
Size: 1155 6961
NspI r_CATG'y
Cuts at: 1132 1229 5474 7019 1132
Size: 97 4245 1545 2229
Fragments arranged by size:
4245 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8116
PciI A'CATG_T
Cuts at: 7015 7015
Size: 8116
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8116
PfoI T'CCnGG_A
Cuts at: 2984 3219 4389 5239 2984
Size: 235 1170 850 5861
Fragments arranged by size:
5861 1170 850 235
PmeI GTTT'AAAC
Cuts at: 5420 5420
Size: 8116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6185 6217 7698 7730 6185
Size: 32 1481 32 6571
Fragments arranged by size:
6571 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8116
PsiI TTA'TAA
Cuts at: 5686 5686
Size: 8116
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3786 3818 3786
Size: 32 8084
PstI C_TGCA'G
Cuts at: 830 2161 2170 2475 2944 3270 4415 4547 4777
Size: 1331 9 305 469 326 1145 132 230
Cuts at: 4777 4831 5364 5468 830
Size: 54 533 104 3478
Fragments arranged by size:
3478 1331 1145 533 469 326 305 230
132 104 54 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8116
PvuII CAG'CTG
Cuts at: 2227 2227
Size: 8116
RsrII CG'GwC_CG
Cuts at: 4011 4011
Size: 8116
SacI G_AGCT'C
Cuts at: 2814 4715 2814
Size: 1901 6215
SalI G'TCGA_C
Cuts at: 1936 5458 1936
Size: 3522 4594
SbfI CC_TGCA'GG
Cuts at: 5468 5468
Size: 8116
ScaI AGT'ACT
Cuts at: 6364 8076 6364
Size: 1712 6404
SexAI A'CCwGG_T
Cuts at: 2139 5073 2139
Size: 2934 5182
SfcI C'TryA_G
Cuts at: 826 1045 2157 2166 2244 2471 2940 3266 3647
Size: 219 1112 9 78 227 469 326 381
Cuts at: 3647 3880 4411 4543 4773 4827 5360 5464 6599
Size: 233 531 132 230 54 533 104 1135
Cuts at: 6599 7280 7471 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1112 681 533 531 469 381
326 233 230 227 219 191 132 104
78 54 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2180 2180
Size: 8116
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8116
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8116
SmaI CCC'GGG
Cuts at: 1872 5443 1872
Size: 3571 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4985 5315 6220 7121 7383 820
Size: 197 926 3042 330 905 901 262 1553
Fragments arranged by size:
3042 1553 926 905 901 330 262 197
SnaBI TAC'GTA
Cuts at: 493 3851 5907 493
Size: 3358 2056 2702
Fragments arranged by size:
3358 2702 2056
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8116
SphI G_CATG'C
Cuts at: 1132 5474 1132
Size: 4342 3774
SspI AAT'ATT
Cuts at: 5 52 2818 6040 5
Size: 47 2766 3222 2081
Fragments arranged by size:
3222 2766 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8116
StyI C'CwwG_G
Cuts at: 513 1065 4518 4914 5555 513
Size: 552 3453 396 641 3074
Fragments arranged by size:
3453 3074 641 552 396
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3730 4691 4811 5045 6143 6160 6313
Size: 1255 2150 961 120 234 1098 17 153
Cuts at: 6313 6498 6943 325
Size: 185 445 1498
Fragments arranged by size:
2150 1498 1255 1098 961 445 234 185
153 120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2501 4555 4853
Size: 274 80 33 51 1443 524 2054 298
Cuts at: 4853 6362 8074 96
Size: 1509 1712 138
Fragments arranged by size:
2054 1712 1509 1443 524 298 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2898 2930 3018 3050 5020 5052 5202 5234 8076
Size: 32 88 32 1970 32 150 32 2842
Cuts at: 8076 8108 2898
Size: 32 2906
Fragments arranged by size:
2906 2842 1970 150 88 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8116
XbaI T'CTAG_A
Cuts at: 4435 5452 4435
Size: 1017 7099
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2147 2147
Size: 8116
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8116
XmaI C'CCGG_G
Cuts at: 1870 5441 1870
Size: 3571 4545
XmnI GAAnn'nnTTC
Cuts at: 2420 6245 2420
Size: 3825 4291
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5924 276
Size: 53 83 186 936 4390 2468
Fragments arranged by size:
4390 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI Bsu36I EcoO109I FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI SacII SanDI SapI SfoI SrfI
SwaI