Restriction map for
pFN21AE4270
(Circular) (Six-base) MAPSORT of: pFN21AE4270.seq Check: 2348 from: 1 to: 8080
pFN21AE4270 8080 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4105 4105
Size: 8080
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5890 278
Size: 53 83 186 936 4354 2468
Fragments arranged by size:
4354 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5423 1937
Size: 3486 4594
Acc65I G'GTAC_C
Cuts at: 3733 5401 3733
Size: 1668 6412
AclI AA'CG_TT
Cuts at: 1563 6207 6580 1563
Size: 4644 373 3063
Fragments arranged by size:
4644 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3405 3710 4553 4807 6143 7527 7795 1632
Size: 1773 305 843 254 1336 1384 268 1917
Fragments arranged by size:
1917 1773 1384 1336 843 305 268 254
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7883
AflIII A'CryG_T
Cuts at: 1203 3255 3949 6979 7956 1203
Size: 2052 694 3030 977 1327
Fragments arranged by size:
3030 2052 1327 977 694
AgeI A'CCGG_T
Cuts at: 6911 6911
Size: 8080
AhdI GACnn_n'nnGTC
Cuts at: 2911 6809 2911
Size: 3898 4182
AleI CACnn'nnGTG
Cuts at: 1166 4824 1166
Size: 3658 4422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8080
AlwNI CAG_nnn'CTG
Cuts at: 5823 7395 5823
Size: 1572 6508
ApaLI G'TGCA_C
Cuts at: 6137 7293 6137
Size: 1156 6924
ApoI r'AATT_y
Cuts at: 1493 2819 3338 4373 5389 5615 5767 7876 1493
Size: 1326 519 1035 1016 226 152 2109 1697
Fragments arranged by size:
2109 1697 1326 1035 1016 519 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6634 160
Size: 872 5602 1606
Fragments arranged by size:
5602 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8080
AvaI C'yCGr_G
Cuts at: 1870 1943 3738 3792 5405 1870
Size: 73 1795 54 1613 4545
Fragments arranged by size:
4545 1795 1613 73 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3971 4004 4181 4214 3971
Size: 33 177 33 7837
Fragments arranged by size:
7837 177 33 33
BamHI G'GATC_C
Cuts at: 3605 3787 5410 5777 3605
Size: 182 1623 367 5908
Fragments arranged by size:
5908 1623 367 182
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3733 4643 4702 5401 6856
Size: 325 217 627 1946 910 59 699 1455
Cuts at: 6856 618
Size: 1842
Fragments arranged by size:
1946 1842 1455 910 699 627 325 217
59
BanII G_rGCy'C
Cuts at: 1391 2193 2511 4717 5399 1391
Size: 802 318 2206 682 4072
Fragments arranged by size:
4072 2206 802 682 318
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2283 2796 3379 4392 928
Size: 971 384 513 583 1013 4616
Fragments arranged by size:
4616 1013 971 583 513 384
BbvCI CC'TCA_GC
Cuts at: 2532 5205 2532
Size: 2673 5407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4092 4126 6271 6305 1903
Size: 34 50 34 2071 34 2145 34 3678
Fragments arranged by size:
3678 2145 2071 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5972 7182 5972
Size: 1210 6870
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8080
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2471 6691 136
Size: 107 122 71 631 605 799 4220 1525
Fragments arranged by size:
4220 1525 799 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3694 4564 8075 3694
Size: 870 3511 3699
Fragments arranged by size:
3699 3511 870
BlpI GC'TnA_GC
Cuts at: 2342 5497 2342
Size: 3155 4925
Bme1580I G_kGCm'C
Cuts at: 1790 6141 7297 1790
Size: 4351 1156 2573
Fragments arranged by size:
4351 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8080
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5361 5811 6769
Size: 416 53 83 399 24 3944 450 958
Cuts at: 6769 442
Size: 1753
Fragments arranged by size:
3944 1753 958 450 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8080
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2493 2493
Size: 8080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2967 3011 3057 6740 1239
Size: 1262 466 44 46 3683 2579
Fragments arranged by size:
3683 2579 1262 466 46 44
Bpu10I CC'TnA_GC
Cuts at: 2232 2241 2532 5205 2232
Size: 9 291 2673 5107
Fragments arranged by size:
5107 2673 291 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2694 3400 5023 6205 7070 7368 2694
Size: 706 1623 1182 865 298 3406
Fragments arranged by size:
3406 1623 1182 865 706 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2925 3033 4611 6743 882
Size: 617 1426 108 1578 2132 2219
Fragments arranged by size:
2219 2132 1578 1426 617 108
BsaAI yAC'GTr
Cuts at: 493 1204 4955 5871 493
Size: 711 3751 916 2702
Fragments arranged by size:
3751 2702 916 711
BsaBI GATnn'nnATC
Cuts at: 3927 8074 3927
Size: 4147 3933
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5887 6269 6904 275
Size: 53 83 186 936 4354 382 635 1451
Fragments arranged by size:
4354 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2902 4366 6513 6911 7185 7332 7743 2902
Size: 1464 2147 398 274 147 411 3239
Fragments arranged by size:
3239 2147 1464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2687 2717 3924 3954 4336
Size: 30 165 30 1284 30 1207 30 382
Cuts at: 4336 4366 1178
Size: 30 4892
Fragments arranged by size:
4892 1284 1207 382 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 2370 3102 3105 3294 3810 3900 4161
Size: 997 187 732 3 189 516 90 261
Cuts at: 4161 4221 4224 5340 1186
Size: 60 3 1116 3926
Fragments arranged by size:
3926 1116 997 732 516 261 189 187
90 60 3 3
BseYI C'CCAG_C
Cuts at: 3043 3545 4239 4293 4400 4770 4947 7283 7779
Size: 502 694 54 107 370 177 2336 496
Cuts at: 7779 3043
Size: 3344
Fragments arranged by size:
3344 2336 694 502 496 370 177 107
54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2888 3468 5048 1163
Size: 573 1152 580 1580 4195
Fragments arranged by size:
4195 1580 1152 580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5530 6291 7319 1540
Size: 275 186 3529 761 1028 2301
Fragments arranged by size:
3529 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 2233 2511 3601 4717 6141 6226 7297 2193
Size: 40 278 1090 1116 1424 85 1071 2976
Fragments arranged by size:
2976 1424 1116 1090 1071 278 85 40
BsmI GAATG_Cn'
Cuts at: 5591 5684 5591
Size: 93 7987
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2233 2511 3601 4717 5399 6141
Size: 399 403 40 278 1090 1116 682 742
Cuts at: 6141 6226 7297 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1116 1090 1071 742 682 403 399
278 85 40
BspEI T'CCGG_A
Cuts at: 4366 7743 4366
Size: 3377 4703
BspHI T'CATG_A
Cuts at: 5969 7699 5969
Size: 1730 6350
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3627 3913 4105 5435 844
Size: 2783 286 192 1330 3489
Fragments arranged by size:
3489 2783 1330 286 192
BsrBI CCG'CTC
Cuts at: 1394 1823 2341 5967 1394
Size: 429 518 3626 3507
Fragments arranged by size:
3626 3507 518 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2210 2604 6575 6749 65
Size: 1183 962 394 3971 174 1396
Fragments arranged by size:
3971 1396 1183 962 394 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4221 6724 6911 1700
Size: 110 2411 2503 187 2869
Fragments arranged by size:
2869 2503 2411 187 110
BsrGI T'GTAC_A
Cuts at: 96 2011 3305 96
Size: 1915 1294 4871
Fragments arranged by size:
4871 1915 1294
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8080
BssSI C'ACGA_G
Cuts at: 2222 3291 6140 7152 2222
Size: 1069 2849 1012 3150
Fragments arranged by size:
3150 2849 1069 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2124 2151 794
Size: 1330 27 6723
Fragments arranged by size:
6723 1330 27
BstBI TT'CG_AA
Cuts at: 2080 6884 2080
Size: 4804 3276
BstEII G'GTnAC_C
Cuts at: 1184 2773 1184
Size: 1589 6491
BstXI CCAn_nnnn'nTGG
Cuts at: 2095 2588 3035 4955 2095
Size: 493 447 1920 5220
Fragments arranged by size:
5220 1920 493 447
BstYI r'GATC_y
Cuts at: 1972 3454 3605 3694 3787 4564 5410 5777 6162
Size: 1482 151 89 93 777 846 367 385
Cuts at: 6162 6179 7631 7717 7729 8075 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1482 1452 846 777 385 367 346
151 93 89 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4707 5319 4707
Size: 612 7468
BtgI C'CryG_G
Cuts at: 513 1065 2699 4762 513
Size: 552 1634 2063 3831
Fragments arranged by size:
3831 2063 1634 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4253 487
Size: 683 56 3027 4314
Fragments arranged by size:
4314 3027 683 56
BtsI GCAGTG_nn'
Cuts at: 2353 3456 5236 5596 6390 6410 2353
Size: 1103 1780 360 794 20 4023
Fragments arranged by size:
4023 1780 1103 794 360 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8080
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8045
DraI TTT'AAA
Cuts at: 2616 5384 2616
Size: 2768 5312
DraIII CAC_nnn'GTG
Cuts at: 3002 3863 5071 3002
Size: 861 1208 6011
Fragments arranged by size:
6011 1208 861
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7087 809
Size: 507 5771 1802
Fragments arranged by size:
5771 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 4018 4857
Size: 54 1425 50 275 18 274 1914 839
Cuts at: 4857 5527 6416 8045 8
Size: 670 889 1629 43
Fragments arranged by size:
1914 1629 1425 889 839 670 275 274
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5527 1537
Size: 275 3715 4090
Fragments arranged by size:
4090 3715 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2548 2800 4050 4570 5084 6010 1353
Size: 1195 252 1250 520 514 926 3423
Fragments arranged by size:
3423 1250 1195 926 520 514 252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3795 6667 7041 7187 387
Size: 1064 138 2206 2872 374 146 1280
Fragments arranged by size:
2872 2206 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2191 2509 4715 2191
Size: 318 2206 5556
Fragments arranged by size:
5556 2206 318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 2967 3011 3057 3405 3710 4553
Size: 393 869 466 44 46 348 305 843
Cuts at: 4553 4807 6143 6740 7527 7795 1239
Size: 254 1336 597 787 268 1524
Fragments arranged by size:
1524 1336 869 843 787 597 466 393
348 305 268 254 46 44
EcoNI CCTnn'n_nnAGG
Cuts at: 5309 5322 5309
Size: 13 8067
EcoO109I rG'GnC_Cy
Cuts at: 2236 5209 5314 2236
Size: 2973 105 5002
Fragments arranged by size:
5002 2973 105
EcoRI G'AATT_C
Cuts at: 5389 5767 5389
Size: 378 7702
FspI TGC'GCA
Cuts at: 6586 6586
Size: 8080
HaeII r_GCGC'y
Cuts at: 1678 3361 7227 1678
Size: 1683 3866 2531
Fragments arranged by size:
3866 2531 1683
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 2777 2809 2897
Size: 32 135 32 452 32 721 32 88
Cuts at: 2897 2929 3721 3753 4144 4176 4826 4858 5281
Size: 32 792 32 391 32 650 32 423
Cuts at: 5281 5313 6721 6753 6795 6827 1373
Size: 32 1408 32 42 32 2626
Fragments arranged by size:
2626 1408 792 721 650 452 423 391
135 88 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2435 3398 5424 5670 669
Size: 988 281 497 963 2026 246 3079
Fragments arranged by size:
3079 2026 988 963 497 281 246
HindIII A'AGCT_T
Cuts at: 748 2411 2610 748
Size: 1663 199 6218
Fragments arranged by size:
6218 1663 199
HpaI GTT'AAC
Cuts at: 5670 5670
Size: 8080
KpnI G_GTAC'C
Cuts at: 3737 5405 3737
Size: 1668 6412
MfeI C'AATT_G
Cuts at: 3018 4919 5679 3018
Size: 1901 760 5419
Fragments arranged by size:
5419 1901 760
MluI A'CGCG_T
Cuts at: 7956 7956
Size: 8080
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3904 4202 7194 7378 1305
Size: 2599 298 2992 184 2007
Fragments arranged by size:
2992 2599 2007 298 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2106 4859 10
Size: 54 1425 343 274 2753 3231
Fragments arranged by size:
3231 2753 1425 343 274 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3256 3859 4014 4824 6038 6397 6556
Size: 648 2090 603 155 810 1214 359 159
Cuts at: 6556 518
Size: 2042
Fragments arranged by size:
2090 2042 1214 810 648 603 359 159
155
MspA1I CmG'CkG
Cuts at: 1606 2809 3133 3303 5496 6173 7321 7566 1606
Size: 1203 324 170 2193 677 1148 245 2120
Fragments arranged by size:
2193 2120 1203 1148 677 324 245 170
NaeI GCC'GGC
Cuts at: 4223 4223
Size: 8080
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7528
NdeI CA'TA_TG
Cuts at: 387 2952 4753 387
Size: 2565 1801 3714
Fragments arranged by size:
3714 2565 1801
NgoMIV G'CCGG_C
Cuts at: 4221 4221
Size: 8080
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8080
NotI GC'GGCC_GC
Cuts at: 5527 5527
Size: 8080
NspI r_CATG'y
Cuts at: 1132 1229 2738 3259 5438 6983 1132
Size: 97 1509 521 2179 1545 2229
Fragments arranged by size:
2229 2179 1545 1509 521 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8080
PciI A'CATG_T
Cuts at: 3255 6979 3255
Size: 3724 4356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2553 1108
Size: 1445 6635
PfoI T'CCnGG_A
Cuts at: 3601 3601
Size: 8080
PmeI GTTT'AAAC
Cuts at: 5384 5384
Size: 8080
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6149 6181 7662 7694 6149
Size: 32 1481 32 6535
Fragments arranged by size:
6535 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2236 5209 5314 2236
Size: 2973 105 5002
Fragments arranged by size:
5002 2973 105
PshAI GACnn'nnGTC
Cuts at: 1613 2423 3099 1613
Size: 810 676 6594
Fragments arranged by size:
6594 810 676
PsiI TTA'TAA
Cuts at: 5650 5650
Size: 8080
PspXI vC'TCGA_Gb
Cuts at: 3738 3792 3738
Size: 54 8026
PstI C_TGCA'G
Cuts at: 830 2220 4102 5432 830
Size: 1390 1882 1330 3478
Fragments arranged by size:
3478 1882 1390 1330
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8080
PvuII CAG'CTG
Cuts at: 2809 3133 3303 2809
Size: 324 170 7586
Fragments arranged by size:
7586 324 170
SacI G_AGCT'C
Cuts at: 2193 2511 4717 2193
Size: 318 2206 5556
Fragments arranged by size:
5556 2206 318
SalI G'TCGA_C
Cuts at: 1936 5422 1936
Size: 3486 4594
SapI GCTCTTCn'nnn_
Cuts at: 4570 4570
Size: 8080
SbfI CC_TGCA'GG
Cuts at: 5432 5432
Size: 8080
ScaI AGT'ACT
Cuts at: 6328 8040 6328
Size: 1712 6368
SexAI A'CCwGG_T
Cuts at: 5067 5067
Size: 8080
SfcI C'TryA_G
Cuts at: 826 1045 2216 4098 5428 6563 7244 7435 826
Size: 219 1171 1882 1330 1135 681 191 1471
Fragments arranged by size:
1882 1471 1330 1171 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8080
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8080
SmaI CCC'GGG
Cuts at: 1872 5407 1872
Size: 3535 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2709 3379 3738 3792 5038 6184
Size: 197 926 766 670 359 54 1246 1146
Cuts at: 6184 7085 7347 820
Size: 901 262 1553
Fragments arranged by size:
1553 1246 1146 926 901 766 670 359
262 197 54
SnaBI TAC'GTA
Cuts at: 493 5871 493
Size: 5378 2702
SpeI A'CTAG_T
Cuts at: 152 2437 152
Size: 2285 5795
SphI G_CATG'C
Cuts at: 1132 5438 1132
Size: 4306 3774
SspI AAT'ATT
Cuts at: 5 52 6004 5
Size: 47 5952 2081
Fragments arranged by size:
5952 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8080
StyI C'CwwG_G
Cuts at: 513 1065 2587 3680 5519 513
Size: 552 1522 1093 1839 3074
Fragments arranged by size:
3074 1839 1522 1093 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2216 4635 6107 6124 6277 6462 6907
Size: 1255 636 2419 1472 17 153 185 445
Cuts at: 6907 325
Size: 1498
Fragments arranged by size:
2419 1498 1472 1255 636 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2011 2328 3305
Size: 274 80 33 51 1443 34 317 977
Cuts at: 3305 6326 8038 96
Size: 3021 1712 138
Fragments arranged by size:
3021 1712 1443 977 317 274 138 80
51 34 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3924 3956 5105 5137 8040 8072 3924
Size: 32 1149 32 2903 32 3932
Fragments arranged by size:
3932 2903 1149 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8080
XbaI T'CTAG_A
Cuts at: 4255 5416 4255
Size: 1161 6919
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4498 5019 4498
Size: 521 7559
XhoI C'TCGA_G
Cuts at: 1943 3738 3792 1943
Size: 1795 54 6231
Fragments arranged by size:
6231 1795 54
XmaI C'CCGG_G
Cuts at: 1870 5405 1870
Size: 3535 4545
XmnI GAAnn'nnTTC
Cuts at: 2076 3339 3630 6209 2076
Size: 1263 291 2579 3947
Fragments arranged by size:
3947 2579 1263 291
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5888 276
Size: 53 83 186 936 4354 2468
Fragments arranged by size:
4354 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI