Restriction map for
pFN21AE4265
(Circular) (Six-base) MAPSORT of: pFN21AE4265.seq Check: 3951 from: 1 to: 7891
pFN21AE4265 7891 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5701 278
Size: 53 83 186 936 4165 2468
Fragments arranged by size:
4165 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5234 1937
Size: 3297 4594
Acc65I G'GTAC_C
Cuts at: 3078 3831 5212 3078
Size: 753 1381 5757
Fragments arranged by size:
5757 1381 753
AclI AA'CG_TT
Cuts at: 1563 4977 6018 6391 1563
Size: 3414 1041 373 3063
Fragments arranged by size:
3414 3063 1041 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3165 3524 3695 5105 5954 7338 7606 1632
Size: 1533 359 171 1410 849 1384 268 1917
Fragments arranged by size:
1917 1533 1410 1384 849 359 268 171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7694
AflIII A'CryG_T
Cuts at: 1203 3967 6790 7767 1203
Size: 2764 2823 977 1327
Fragments arranged by size:
2823 2764 1327 977
AgeI A'CCGG_T
Cuts at: 6722 6722
Size: 7891
AhdI GACnn_n'nnGTC
Cuts at: 4449 4588 6620 4449
Size: 139 2032 5720
Fragments arranged by size:
5720 2032 139
AleI CACnn'nnGTG
Cuts at: 1166 3018 4149 1166
Size: 1852 1131 4908
Fragments arranged by size:
4908 1852 1131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4995 1184
Size: 3811 4080
AlwNI CAG_nnn'CTG
Cuts at: 2149 2281 3233 3665 5634 7206 2149
Size: 132 952 432 1969 1572 2834
Fragments arranged by size:
2834 1969 1572 952 432 132
ApaLI G'TGCA_C
Cuts at: 5948 7104 5948
Size: 1156 6735
ApoI r'AATT_y
Cuts at: 1493 2073 3598 5200 5426 5578 7687 1493
Size: 580 1525 1602 226 152 2109 1697
Fragments arranged by size:
2109 1697 1602 1525 580 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6445 160
Size: 872 5413 1606
Fragments arranged by size:
5413 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7891
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 4614 5216 1870
Size: 73 71 2600 602 4545
Fragments arranged by size:
4545 2600 602 73 71
AvrII C'CTAG_G
Cuts at: 4585 4585
Size: 7891
BamHI G'GATC_C
Cuts at: 5221 5588 5221
Size: 367 7524
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3078 3831 4235 4309 5062
Size: 325 217 627 1291 753 404 74 753
Cuts at: 5062 5212 6667 618
Size: 150 1455 1842
Fragments arranged by size:
1842 1455 1291 753 753 627 404 325
217 150 74
BanII G_rGCy'C
Cuts at: 1391 2083 2845 2937 3069 4143 4994 5024 5210
Size: 692 762 92 132 1074 851 30 186
Cuts at: 5210 1391
Size: 4072
Fragments arranged by size:
4072 1074 851 762 692 186 132 92
30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2596 3530 4416 928
Size: 971 697 934 886 4403
Fragments arranged by size:
4403 971 934 886 697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6082 6116 1903
Size: 34 4145 34 3678
Fragments arranged by size:
4145 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4900 5783 6993 4900
Size: 883 1210 5798
Fragments arranged by size:
5798 1210 883
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7891
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6502 136
Size: 107 122 71 631 605 4830 1525
Fragments arranged by size:
4830 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2588 7886 2588
Size: 5298 2593
BlpI GC'TnA_GC
Cuts at: 5308 5308
Size: 7891
Bme1580I G_kGCm'C
Cuts at: 1790 3046 5952 7108 1790
Size: 1256 2906 1156 2573
Fragments arranged by size:
2906 2573 1256 1156
BmgBI CAC'GTC
Cuts at: 1318 4752 1318
Size: 3434 4457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5622 6580 442
Size: 416 53 83 399 24 4205 958 1753
Fragments arranged by size:
4205 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2129 2274 2869 4086 4488 4883 4934
Size: 887 3 145 595 1217 402 395 51
Cuts at: 4934 5011 5076 6551 1239
Size: 77 65 1475 2579
Fragments arranged by size:
2579 1475 1217 887 595 402 395 145
77 65 51 3
Bpu10I CC'TnA_GC
Cuts at: 3074 4656 4812 3074
Size: 1582 156 6153
Fragments arranged by size:
6153 1582 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2374 4700 6016 6881 7179 2374
Size: 2326 1316 865 298 3086
Fragments arranged by size:
3086 2326 1316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6554 882
Size: 617 5055 2219
Fragments arranged by size:
5055 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5682 493
Size: 711 4478 2702
Fragments arranged by size:
4478 2702 711
BsaBI GATnn'nnATC
Cuts at: 7885 7885
Size: 7891
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4513 5698 6080 6715
Size: 53 83 186 936 2980 1185 382 635
Cuts at: 6715 275
Size: 1451
Fragments arranged by size:
2980 1451 1185 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6324 6722 6996 7143 7554 6324
Size: 398 274 147 411 6661
Fragments arranged by size:
6661 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2578 2608 4421 4451 1178
Size: 30 165 30 1175 30 1813 30 4618
Fragments arranged by size:
4618 1813 1175 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2636 3782 4503 4564 5014 1186
Size: 1450 1146 721 61 450 4063
Fragments arranged by size:
4063 1450 1146 721 450 61
BseYI C'CCAG_C
Cuts at: 3045 3272 7094 7590 3045
Size: 227 3822 496 3346
Fragments arranged by size:
3822 3346 496 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2556 4352 5099 1163
Size: 573 820 1796 747 3955
Fragments arranged by size:
3955 1796 820 747 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5341 6102 7130 1540
Size: 275 186 3340 761 1028 2301
Fragments arranged by size:
3340 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2083 3069 4101 4877 4994 5024 5952 6037 7108
Size: 986 1032 776 117 30 928 85 1071
Cuts at: 7108 2083
Size: 2866
Fragments arranged by size:
2866 1071 1032 986 928 776 117 85
30
BsmI GAATG_Cn'
Cuts at: 2444 2920 3209 5402 5495 2444
Size: 476 289 2193 93 4840
Fragments arranged by size:
4840 2193 476 289 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2177 2177
Size: 7891
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2845 2937 3046 3069 4101 4143
Size: 399 293 762 92 109 23 1032 42
Cuts at: 4143 4877 4994 5024 5210 5952 6037 7108 1391
Size: 734 117 30 186 742 85 1071 2174
Fragments arranged by size:
2174 1071 1032 762 742 734 399 293
186 117 109 92 85 42 30 23
BspEI T'CCGG_A
Cuts at: 7554 7554
Size: 7891
BspHI T'CATG_A
Cuts at: 2455 2782 3324 5780 7510 2455
Size: 327 542 2456 1730 2836
Fragments arranged by size:
2836 2456 1730 542 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5119 5155 5246 844
Size: 4275 36 91 3489
Fragments arranged by size:
4275 3489 91 36
BsrBI CCG'CTC
Cuts at: 1394 1823 4546 4660 5778 1394
Size: 429 2723 114 1118 3507
Fragments arranged by size:
3507 2723 1118 429 114
BsrDI GCAATG_nn'
Cuts at: 65 1248 4537 6386 6560 65
Size: 1183 3289 1849 174 1396
Fragments arranged by size:
3289 1849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4701 6535 6722 1700
Size: 110 2891 1834 187 2869
Fragments arranged by size:
2891 2869 1834 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7891
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7891
BssSI C'ACGA_G
Cuts at: 5017 5951 6963 5017
Size: 934 1012 5945
Fragments arranged by size:
5945 1012 934
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4886 794
Size: 4092 3799
BstBI TT'CG_AA
Cuts at: 6695 6695
Size: 7891
BstEII G'GTnAC_C
Cuts at: 1184 3175 1184
Size: 1991 5900
BstXI CCAn_nnnn'nTGG
Cuts at: 4130 4130
Size: 7891
BstYI r'GATC_y
Cuts at: 1972 2146 2588 5221 5588 5973 5990 7442 7528
Size: 174 442 2633 367 385 17 1452 86
Cuts at: 7528 7540 7886 1972
Size: 12 346 1977
Fragments arranged by size:
2633 1977 1452 442 385 367 346 174
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3714 4985 5099 3714
Size: 1271 114 6506
Fragments arranged by size:
6506 1271 114
BtgI C'CryG_G
Cuts at: 513 1065 3688 4633 4678 4950 513
Size: 552 2623 945 45 272 3454
Fragments arranged by size:
3454 2623 945 552 272 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3262 3885 487
Size: 683 56 2036 623 4493
Fragments arranged by size:
4493 2036 683 623 56
BtsI GCAGTG_nn'
Cuts at: 4399 5407 6201 6221 4399
Size: 1008 794 20 6069
Fragments arranged by size:
6069 1008 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7891
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7856
DraI TTT'AAA
Cuts at: 2425 5195 2425
Size: 2770 5121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6898 809
Size: 507 5582 1802
Fragments arranged by size:
5582 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3900 4534 4675
Size: 54 1425 50 275 18 2070 634 141
Cuts at: 4675 5338 6227 7856 8
Size: 663 889 1629 43
Fragments arranged by size:
2070 1629 1425 889 663 634 275 141
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5338 1537
Size: 275 3526 4090
Fragments arranged by size:
4090 3526 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3120 3283 5821 1353
Size: 1767 163 2538 3423
Fragments arranged by size:
3423 2538 1767 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6478 6852 6998 387
Size: 1064 138 4889 374 146 1280
Fragments arranged by size:
4889 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2081 3067 4992 5022 2081
Size: 986 1925 30 4950
Fragments arranged by size:
4950 1925 986 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2129 2274 2869 3165 3524 3695
Size: 393 494 3 145 595 296 359 171
Cuts at: 3695 4086 4488 4883 4934 5011 5076 5105 5954
Size: 391 402 395 51 77 65 29 849
Cuts at: 5954 6551 7338 7606 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 849 787 597 595 494 402 395
393 391 359 296 268 171 145 77
65 51 29 3
EcoNI CCTnn'n_nnAGG
Cuts at: 5139 5139
Size: 7891
EcoO109I rG'GnC_Cy
Cuts at: 3399 4590 4618 5144 3399
Size: 1191 28 526 6146
Fragments arranged by size:
6146 1191 526 28
EcoRI G'AATT_C
Cuts at: 5200 5578 5200
Size: 378 7513
FspI TGC'GCA
Cuts at: 6397 6397
Size: 7891
HaeII r_GCGC'y
Cuts at: 1678 3776 7038 1678
Size: 2098 3262 2531
Fragments arranged by size:
3262 2531 2098
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2993 3025 3641 3673 3938
Size: 32 135 32 1421 32 616 32 265
Cuts at: 3938 3970 4403 4421 4435 4453 4467 4574 4606
Size: 32 433 18 14 18 14 107 32
Cuts at: 4606 4795 4827 6532 6564 6606 6638 1373
Size: 189 32 1705 32 42 32 2626
Fragments arranged by size:
2626 1705 1421 616 433 265 189 135
107 42 32 32 32 32 32 32
32 32 32 18 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4582 5235 5481 669
Size: 988 281 2644 653 246 3079
Fragments arranged by size:
3079 2644 988 653 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7891
HpaI GTT'AAC
Cuts at: 5481 5481
Size: 7891
KpnI G_GTAC'C
Cuts at: 3082 3835 5216 3082
Size: 753 1381 5757
Fragments arranged by size:
5757 1381 753
MfeI C'AATT_G
Cuts at: 4806 5490 4806
Size: 684 7207
MluI A'CGCG_T
Cuts at: 7767 7767
Size: 7891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2473 5192 7005 7189 1305
Size: 1168 2719 1813 184 2007
Fragments arranged by size:
2719 2007 1813 1168 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3902 4536 4677 10
Size: 54 1425 343 2070 634 141 3224
Fragments arranged by size:
3224 2070 1425 634 343 141 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2963 3018 3972 4149 5849 6208 6367
Size: 648 1797 55 954 177 1700 359 159
Cuts at: 6367 518
Size: 2042
Fragments arranged by size:
2042 1797 1700 954 648 359 177 159
55
MspA1I CmG'CkG
Cuts at: 1606 5083 5307 5984 7132 7377 1606
Size: 3477 224 677 1148 245 2120
Fragments arranged by size:
3477 2120 1148 677 245 224
NaeI GCC'GGC
Cuts at: 4703 4703
Size: 7891
NcoI C'CATG_G
Cuts at: 513 1065 4678 513
Size: 552 3613 3726
Fragments arranged by size:
3726 3613 552
NdeI CA'TA_TG
Cuts at: 387 2511 387
Size: 2124 5767
NgoMIV G'CCGG_C
Cuts at: 4701 4701
Size: 7891
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7891
NotI GC'GGCC_GC
Cuts at: 5338 5338
Size: 7891
NspI r_CATG'y
Cuts at: 1132 1229 3971 4562 4889 5249 6794 1132
Size: 97 2742 591 327 360 1545 2229
Fragments arranged by size:
2742 2229 1545 591 360 327 97
PasI CC'CwG_GG
Cuts at: 1348 3403 5161 1348
Size: 2055 1758 4078
Fragments arranged by size:
4078 2055 1758
PciI A'CATG_T
Cuts at: 3967 6790 3967
Size: 2823 5068
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7891
PfoI T'CCnGG_A
Cuts at: 2142 2195 2142
Size: 53 7838
PmeI GTTT'AAAC
Cuts at: 5195 5195
Size: 7891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5960 5992 7473 7505 5960
Size: 32 1481 32 6346
Fragments arranged by size:
6346 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3399 4590 5144 3399
Size: 1191 554 6146
Fragments arranged by size:
6146 1191 554
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7891
PsiI TTA'TAA
Cuts at: 3894 5461 3894
Size: 1567 6324
PstI C_TGCA'G
Cuts at: 830 3396 4629 5243 830
Size: 2566 1233 614 3478
Fragments arranged by size:
3478 2566 1233 614
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7891
PvuII CAG'CTG
Cuts at: 5083 5083
Size: 7891
SacI G_AGCT'C
Cuts at: 2083 3069 4994 5024 2083
Size: 986 1925 30 4950
Fragments arranged by size:
4950 1925 986 30
SalI G'TCGA_C
Cuts at: 1936 5233 1936
Size: 3297 4594
SanDI GG'GwC_CC
Cuts at: 4590 4590
Size: 7891
SapI GCTCTTCn'nnn_
Cuts at: 3283 3283
Size: 7891
SbfI CC_TGCA'GG
Cuts at: 5243 5243
Size: 7891
ScaI AGT'ACT
Cuts at: 2774 5044 6139 7851 2774
Size: 2270 1095 1712 2814
Fragments arranged by size:
2814 2270 1712 1095
SfcI C'TryA_G
Cuts at: 826 1045 3392 4625 5239 6374 7055 7246 826
Size: 219 2347 1233 614 1135 681 191 1471
Fragments arranged by size:
2347 1471 1233 1135 681 614 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7891
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7891
SmaI CCC'GGG
Cuts at: 1872 4616 5218 1872
Size: 2744 602 4545
Fragments arranged by size:
4545 2744 602
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2353 4715 5995 6896 7158 820
Size: 197 926 410 2362 1280 901 262 1553
Fragments arranged by size:
2362 1553 1280 926 901 410 262 197
SnaBI TAC'GTA
Cuts at: 493 5682 493
Size: 5189 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7891
SphI G_CATG'C
Cuts at: 1132 4889 5249 1132
Size: 3757 360 3774
Fragments arranged by size:
3774 3757 360
SspI AAT'ATT
Cuts at: 5 52 2192 2690 3341 5815 5
Size: 47 2140 498 651 2474 2081
Fragments arranged by size:
2474 2140 2081 651 498 47
StuI AGG'CCT
Cuts at: 1517 4672 1517
Size: 3155 4736
StyI C'CwwG_G
Cuts at: 513 1065 4425 4585 4678 5330 513
Size: 552 3360 160 93 652 3074
Fragments arranged by size:
3360 3074 652 552 160 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3008 3802 5080 5918 5935 6088 6273
Size: 1255 1428 794 1278 838 17 153 185
Cuts at: 6273 6718 325
Size: 445 1498
Fragments arranged by size:
1498 1428 1278 1255 838 794 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2772 2868 3864
Size: 274 80 33 51 1443 795 96 996
Cuts at: 3864 5042 6137 7849 96
Size: 1178 1095 1712 138
Fragments arranged by size:
1712 1443 1178 1095 996 795 274 138
96 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2578 2610 3206 3238 7851 7883 2578
Size: 32 596 32 4613 32 2586
Fragments arranged by size:
4613 2586 596 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7891
XbaI T'CTAG_A
Cuts at: 2203 5227 2203
Size: 3024 4867
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2298 2298
Size: 7891
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7891
XmaI C'CCGG_G
Cuts at: 1870 4614 5216 1870
Size: 2744 602 4545
Fragments arranged by size:
4545 2744 602
XmnI GAAnn'nnTTC
Cuts at: 6020 6020
Size: 7891
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5699 276
Size: 53 83 186 936 4165 2468
Fragments arranged by size:
4165 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI
SwaI