(Circular) (Six-base) MAPSORT of: pFN21AE4265.seq Check: 3951 from: 1 to: 7891 pFN21AE4265 7891 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5701 278 Size: 53 83 186 936 4165 2468 Fragments arranged by size: 4165 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5234 1937 Size: 3297 4594 Acc65I G'GTAC_C Cuts at: 3078 3831 5212 3078 Size: 753 1381 5757 Fragments arranged by size: 5757 1381 753 AclI AA'CG_TT Cuts at: 1563 4977 6018 6391 1563 Size: 3414 1041 373 3063 Fragments arranged by size: 3414 3063 1041 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3165 3524 3695 5105 5954 7338 7606 1632 Size: 1533 359 171 1410 849 1384 268 1917 Fragments arranged by size: 1917 1533 1410 1384 849 359 268 171 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7694 AflIII A'CryG_T Cuts at: 1203 3967 6790 7767 1203 Size: 2764 2823 977 1327 Fragments arranged by size: 2823 2764 1327 977 AgeI A'CCGG_T Cuts at: 6722 6722 Size: 7891 AhdI GACnn_n'nnGTC Cuts at: 4449 4588 6620 4449 Size: 139 2032 5720 Fragments arranged by size: 5720 2032 139 AleI CACnn'nnGTG Cuts at: 1166 3018 4149 1166 Size: 1852 1131 4908 Fragments arranged by size: 4908 1852 1131 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4995 1184 Size: 3811 4080 AlwNI CAG_nnn'CTG Cuts at: 2149 2281 3233 3665 5634 7206 2149 Size: 132 952 432 1969 1572 2834 Fragments arranged by size: 2834 1969 1572 952 432 132 ApaLI G'TGCA_C Cuts at: 5948 7104 5948 Size: 1156 6735 ApoI r'AATT_y Cuts at: 1493 2073 3598 5200 5426 5578 7687 1493 Size: 580 1525 1602 226 152 2109 1697 Fragments arranged by size: 2109 1697 1602 1525 580 226 152 AseI AT'TA_AT Cuts at: 160 1032 6445 160 Size: 872 5413 1606 Fragments arranged by size: 5413 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7891 AvaI C'yCGr_G Cuts at: 1870 1943 2014 4614 5216 1870 Size: 73 71 2600 602 4545 Fragments arranged by size: 4545 2600 602 73 71 AvrII C'CTAG_G Cuts at: 4585 4585 Size: 7891 BamHI G'GATC_C Cuts at: 5221 5588 5221 Size: 367 7524 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3078 3831 4235 4309 5062 Size: 325 217 627 1291 753 404 74 753 Cuts at: 5062 5212 6667 618 Size: 150 1455 1842 Fragments arranged by size: 1842 1455 1291 753 753 627 404 325 217 150 74 BanII G_rGCy'C Cuts at: 1391 2083 2845 2937 3069 4143 4994 5024 5210 Size: 692 762 92 132 1074 851 30 186 Cuts at: 5210 1391 Size: 4072 Fragments arranged by size: 4072 1074 851 762 692 186 132 92 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2596 3530 4416 928 Size: 971 697 934 886 4403 Fragments arranged by size: 4403 971 934 886 697 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6082 6116 1903 Size: 34 4145 34 3678 Fragments arranged by size: 4145 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4900 5783 6993 4900 Size: 883 1210 5798 Fragments arranged by size: 5798 1210 883 BclI T'GATC_A Cuts at: 1548 1548 Size: 7891 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6502 136 Size: 107 122 71 631 605 4830 1525 Fragments arranged by size: 4830 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2588 7886 2588 Size: 5298 2593 BlpI GC'TnA_GC Cuts at: 5308 5308 Size: 7891 Bme1580I G_kGCm'C Cuts at: 1790 3046 5952 7108 1790 Size: 1256 2906 1156 2573 Fragments arranged by size: 2906 2573 1256 1156 BmgBI CAC'GTC Cuts at: 1318 4752 1318 Size: 3434 4457 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5622 6580 442 Size: 416 53 83 399 24 4205 958 1753 Fragments arranged by size: 4205 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7891 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2129 2274 2869 4086 4488 4883 4934 Size: 887 3 145 595 1217 402 395 51 Cuts at: 4934 5011 5076 6551 1239 Size: 77 65 1475 2579 Fragments arranged by size: 2579 1475 1217 887 595 402 395 145 77 65 51 3 Bpu10I CC'TnA_GC Cuts at: 3074 4656 4812 3074 Size: 1582 156 6153 Fragments arranged by size: 6153 1582 156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2374 4700 6016 6881 7179 2374 Size: 2326 1316 865 298 3086 Fragments arranged by size: 3086 2326 1316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6554 882 Size: 617 5055 2219 Fragments arranged by size: 5055 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5682 493 Size: 711 4478 2702 Fragments arranged by size: 4478 2702 711 BsaBI GATnn'nnATC Cuts at: 7885 7885 Size: 7891 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4513 5698 6080 6715 Size: 53 83 186 936 2980 1185 382 635 Cuts at: 6715 275 Size: 1451 Fragments arranged by size: 2980 1451 1185 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6324 6722 6996 7143 7554 6324 Size: 398 274 147 411 6661 Fragments arranged by size: 6661 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2578 2608 4421 4451 1178 Size: 30 165 30 1175 30 1813 30 4618 Fragments arranged by size: 4618 1813 1175 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2636 3782 4503 4564 5014 1186 Size: 1450 1146 721 61 450 4063 Fragments arranged by size: 4063 1450 1146 721 450 61 BseYI C'CCAG_C Cuts at: 3045 3272 7094 7590 3045 Size: 227 3822 496 3346 Fragments arranged by size: 3822 3346 496 227 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2556 4352 5099 1163 Size: 573 820 1796 747 3955 Fragments arranged by size: 3955 1796 820 747 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5341 6102 7130 1540 Size: 275 186 3340 761 1028 2301 Fragments arranged by size: 3340 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2083 3069 4101 4877 4994 5024 5952 6037 7108 Size: 986 1032 776 117 30 928 85 1071 Cuts at: 7108 2083 Size: 2866 Fragments arranged by size: 2866 1071 1032 986 928 776 117 85 30 BsmI GAATG_Cn' Cuts at: 2444 2920 3209 5402 5495 2444 Size: 476 289 2193 93 4840 Fragments arranged by size: 4840 2193 476 289 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2177 2177 Size: 7891 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2845 2937 3046 3069 4101 4143 Size: 399 293 762 92 109 23 1032 42 Cuts at: 4143 4877 4994 5024 5210 5952 6037 7108 1391 Size: 734 117 30 186 742 85 1071 2174 Fragments arranged by size: 2174 1071 1032 762 742 734 399 293 186 117 109 92 85 42 30 23 BspEI T'CCGG_A Cuts at: 7554 7554 Size: 7891 BspHI T'CATG_A Cuts at: 2455 2782 3324 5780 7510 2455 Size: 327 542 2456 1730 2836 Fragments arranged by size: 2836 2456 1730 542 327 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5119 5155 5246 844 Size: 4275 36 91 3489 Fragments arranged by size: 4275 3489 91 36 BsrBI CCG'CTC Cuts at: 1394 1823 4546 4660 5778 1394 Size: 429 2723 114 1118 3507 Fragments arranged by size: 3507 2723 1118 429 114 BsrDI GCAATG_nn' Cuts at: 65 1248 4537 6386 6560 65 Size: 1183 3289 1849 174 1396 Fragments arranged by size: 3289 1849 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4701 6535 6722 1700 Size: 110 2891 1834 187 2869 Fragments arranged by size: 2891 2869 1834 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7891 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7891 BssSI C'ACGA_G Cuts at: 5017 5951 6963 5017 Size: 934 1012 5945 Fragments arranged by size: 5945 1012 934 BstAPI GCAn_nnn'nTGC Cuts at: 794 4886 794 Size: 4092 3799 BstBI TT'CG_AA Cuts at: 6695 6695 Size: 7891 BstEII G'GTnAC_C Cuts at: 1184 3175 1184 Size: 1991 5900 BstXI CCAn_nnnn'nTGG Cuts at: 4130 4130 Size: 7891 BstYI r'GATC_y Cuts at: 1972 2146 2588 5221 5588 5973 5990 7442 7528 Size: 174 442 2633 367 385 17 1452 86 Cuts at: 7528 7540 7886 1972 Size: 12 346 1977 Fragments arranged by size: 2633 1977 1452 442 385 367 346 174 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3714 4985 5099 3714 Size: 1271 114 6506 Fragments arranged by size: 6506 1271 114 BtgI C'CryG_G Cuts at: 513 1065 3688 4633 4678 4950 513 Size: 552 2623 945 45 272 3454 Fragments arranged by size: 3454 2623 945 552 272 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3262 3885 487 Size: 683 56 2036 623 4493 Fragments arranged by size: 4493 2036 683 623 56 BtsI GCAGTG_nn' Cuts at: 4399 5407 6201 6221 4399 Size: 1008 794 20 6069 Fragments arranged by size: 6069 1008 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7891 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7856 DraI TTT'AAA Cuts at: 2425 5195 2425 Size: 2770 5121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6898 809 Size: 507 5582 1802 Fragments arranged by size: 5582 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3900 4534 4675 Size: 54 1425 50 275 18 2070 634 141 Cuts at: 4675 5338 6227 7856 8 Size: 663 889 1629 43 Fragments arranged by size: 2070 1629 1425 889 663 634 275 141 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5338 1537 Size: 275 3526 4090 Fragments arranged by size: 4090 3526 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3120 3283 5821 1353 Size: 1767 163 2538 3423 Fragments arranged by size: 3423 2538 1767 163 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6478 6852 6998 387 Size: 1064 138 4889 374 146 1280 Fragments arranged by size: 4889 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2081 3067 4992 5022 2081 Size: 986 1925 30 4950 Fragments arranged by size: 4950 1925 986 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2129 2274 2869 3165 3524 3695 Size: 393 494 3 145 595 296 359 171 Cuts at: 3695 4086 4488 4883 4934 5011 5076 5105 5954 Size: 391 402 395 51 77 65 29 849 Cuts at: 5954 6551 7338 7606 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 849 787 597 595 494 402 395 393 391 359 296 268 171 145 77 65 51 29 3 EcoNI CCTnn'n_nnAGG Cuts at: 5139 5139 Size: 7891 EcoO109I rG'GnC_Cy Cuts at: 3399 4590 4618 5144 3399 Size: 1191 28 526 6146 Fragments arranged by size: 6146 1191 526 28 EcoRI G'AATT_C Cuts at: 5200 5578 5200 Size: 378 7513 FspI TGC'GCA Cuts at: 6397 6397 Size: 7891 HaeII r_GCGC'y Cuts at: 1678 3776 7038 1678 Size: 2098 3262 2531 Fragments arranged by size: 3262 2531 2098 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2993 3025 3641 3673 3938 Size: 32 135 32 1421 32 616 32 265 Cuts at: 3938 3970 4403 4421 4435 4453 4467 4574 4606 Size: 32 433 18 14 18 14 107 32 Cuts at: 4606 4795 4827 6532 6564 6606 6638 1373 Size: 189 32 1705 32 42 32 2626 Fragments arranged by size: 2626 1705 1421 616 433 265 189 135 107 42 32 32 32 32 32 32 32 32 32 18 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 4582 5235 5481 669 Size: 988 281 2644 653 246 3079 Fragments arranged by size: 3079 2644 988 653 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7891 HpaI GTT'AAC Cuts at: 5481 5481 Size: 7891 KpnI G_GTAC'C Cuts at: 3082 3835 5216 3082 Size: 753 1381 5757 Fragments arranged by size: 5757 1381 753 MfeI C'AATT_G Cuts at: 4806 5490 4806 Size: 684 7207 MluI A'CGCG_T Cuts at: 7767 7767 Size: 7891 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2473 5192 7005 7189 1305 Size: 1168 2719 1813 184 2007 Fragments arranged by size: 2719 2007 1813 1168 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3902 4536 4677 10 Size: 54 1425 343 2070 634 141 3224 Fragments arranged by size: 3224 2070 1425 634 343 141 54 MslI CAynn'nnrTG Cuts at: 518 1166 2963 3018 3972 4149 5849 6208 6367 Size: 648 1797 55 954 177 1700 359 159 Cuts at: 6367 518 Size: 2042 Fragments arranged by size: 2042 1797 1700 954 648 359 177 159 55 MspA1I CmG'CkG Cuts at: 1606 5083 5307 5984 7132 7377 1606 Size: 3477 224 677 1148 245 2120 Fragments arranged by size: 3477 2120 1148 677 245 224 NaeI GCC'GGC Cuts at: 4703 4703 Size: 7891 NcoI C'CATG_G Cuts at: 513 1065 4678 513 Size: 552 3613 3726 Fragments arranged by size: 3726 3613 552 NdeI CA'TA_TG Cuts at: 387 2511 387 Size: 2124 5767 NgoMIV G'CCGG_C Cuts at: 4701 4701 Size: 7891 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7891 NotI GC'GGCC_GC Cuts at: 5338 5338 Size: 7891 NspI r_CATG'y Cuts at: 1132 1229 3971 4562 4889 5249 6794 1132 Size: 97 2742 591 327 360 1545 2229 Fragments arranged by size: 2742 2229 1545 591 360 327 97 PasI CC'CwG_GG Cuts at: 1348 3403 5161 1348 Size: 2055 1758 4078 Fragments arranged by size: 4078 2055 1758 PciI A'CATG_T Cuts at: 3967 6790 3967 Size: 2823 5068 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7891 PfoI T'CCnGG_A Cuts at: 2142 2195 2142 Size: 53 7838 PmeI GTTT'AAAC Cuts at: 5195 5195 Size: 7891 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5960 5992 7473 7505 5960 Size: 32 1481 32 6346 Fragments arranged by size: 6346 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3399 4590 5144 3399 Size: 1191 554 6146 Fragments arranged by size: 6146 1191 554 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7891 PsiI TTA'TAA Cuts at: 3894 5461 3894 Size: 1567 6324 PstI C_TGCA'G Cuts at: 830 3396 4629 5243 830 Size: 2566 1233 614 3478 Fragments arranged by size: 3478 2566 1233 614 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7891 PvuII CAG'CTG Cuts at: 5083 5083 Size: 7891 SacI G_AGCT'C Cuts at: 2083 3069 4994 5024 2083 Size: 986 1925 30 4950 Fragments arranged by size: 4950 1925 986 30 SalI G'TCGA_C Cuts at: 1936 5233 1936 Size: 3297 4594 SanDI GG'GwC_CC Cuts at: 4590 4590 Size: 7891 SapI GCTCTTCn'nnn_ Cuts at: 3283 3283 Size: 7891 SbfI CC_TGCA'GG Cuts at: 5243 5243 Size: 7891 ScaI AGT'ACT Cuts at: 2774 5044 6139 7851 2774 Size: 2270 1095 1712 2814 Fragments arranged by size: 2814 2270 1712 1095 SfcI C'TryA_G Cuts at: 826 1045 3392 4625 5239 6374 7055 7246 826 Size: 219 2347 1233 614 1135 681 191 1471 Fragments arranged by size: 2347 1471 1233 1135 681 614 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7891 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7891 SmaI CCC'GGG Cuts at: 1872 4616 5218 1872 Size: 2744 602 4545 Fragments arranged by size: 4545 2744 602 SmlI C'TyrA_G Cuts at: 820 1017 1943 2353 4715 5995 6896 7158 820 Size: 197 926 410 2362 1280 901 262 1553 Fragments arranged by size: 2362 1553 1280 926 901 410 262 197 SnaBI TAC'GTA Cuts at: 493 5682 493 Size: 5189 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7891 SphI G_CATG'C Cuts at: 1132 4889 5249 1132 Size: 3757 360 3774 Fragments arranged by size: 3774 3757 360 SspI AAT'ATT Cuts at: 5 52 2192 2690 3341 5815 5 Size: 47 2140 498 651 2474 2081 Fragments arranged by size: 2474 2140 2081 651 498 47 StuI AGG'CCT Cuts at: 1517 4672 1517 Size: 3155 4736 StyI C'CwwG_G Cuts at: 513 1065 4425 4585 4678 5330 513 Size: 552 3360 160 93 652 3074 Fragments arranged by size: 3360 3074 652 552 160 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3008 3802 5080 5918 5935 6088 6273 Size: 1255 1428 794 1278 838 17 153 185 Cuts at: 6273 6718 325 Size: 445 1498 Fragments arranged by size: 1498 1428 1278 1255 838 794 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2772 2868 3864 Size: 274 80 33 51 1443 795 96 996 Cuts at: 3864 5042 6137 7849 96 Size: 1178 1095 1712 138 Fragments arranged by size: 1712 1443 1178 1095 996 795 274 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2578 2610 3206 3238 7851 7883 2578 Size: 32 596 32 4613 32 2586 Fragments arranged by size: 4613 2586 596 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7891 XbaI T'CTAG_A Cuts at: 2203 5227 2203 Size: 3024 4867 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2298 2298 Size: 7891 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7891 XmaI C'CCGG_G Cuts at: 1870 4614 5216 1870 Size: 2744 602 4545 Fragments arranged by size: 4545 2744 602 XmnI GAAnn'nnTTC Cuts at: 6020 6020 Size: 7891 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5699 276 Size: 53 83 186 936 4165 2468 Fragments arranged by size: 4165 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI