Restriction map for
pFN21AE4263
(Circular) (Six-base) MAPSORT of: pFN21AE4263.seq Check: 8367 from: 1 to: 7909
pFN21AE4263 7909 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5117 5117
Size: 7909
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4563 5719 278
Size: 53 83 186 936 3027 1156 2468
Fragments arranged by size:
3027 2468 1156 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2146 3360 4827 5252 1937
Size: 209 1214 1467 425 4594
Fragments arranged by size:
4594 1467 1214 425 209
Acc65I G'GTAC_C
Cuts at: 5230 5230
Size: 7909
AclI AA'CG_TT
Cuts at: 1563 6036 6409 1563
Size: 4473 373 3063
Fragments arranged by size:
4473 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3582 4943 5972 7356 7624 1632
Size: 1950 1361 1029 1384 268 1917
Fragments arranged by size:
1950 1917 1384 1361 1029 268
AfeI AGC'GCT
Cuts at: 4508 4508
Size: 7909
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7712
AflIII A'CryG_T
Cuts at: 1203 3940 6808 7785 1203
Size: 2737 2868 977 1327
Fragments arranged by size:
2868 2737 1327 977
AgeI A'CCGG_T
Cuts at: 6740 6740
Size: 7909
AhdI GACnn_n'nnGTC
Cuts at: 2203 6638 2203
Size: 4435 3474
AleI CACnn'nnGTG
Cuts at: 1166 2580 1166
Size: 1414 6495
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7909
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4463 4495 4463
Size: 32 7877
AlwNI CAG_nnn'CTG
Cuts at: 3028 5652 7224 3028
Size: 2624 1572 3713
Fragments arranged by size:
3713 2624 1572
ApaI G_GGCC'C
Cuts at: 3516 3516
Size: 7909
ApaLI G'TGCA_C
Cuts at: 2220 5966 7122 2220
Size: 3746 1156 3007
Fragments arranged by size:
3746 3007 1156
ApoI r'AATT_y
Cuts at: 1493 2532 2861 3706 5218 5444 5596 7705 1493
Size: 1039 329 845 1512 226 152 2109 1697
Fragments arranged by size:
2109 1697 1512 1039 845 329 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6463 160
Size: 872 5431 1606
Fragments arranged by size:
5431 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 3509 3839 4157 4730 5234 1870
Size: 73 83 1483 330 318 573 504 4545
Fragments arranged by size:
4545 1483 573 504 330 318 83 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2413 2446 2413
Size: 33 7876
BamHI G'GATC_C
Cuts at: 4737 5239 5606 4737
Size: 502 367 7040
Fragments arranged by size:
7040 502 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3175 3615 4422 5230 6685
Size: 325 217 627 1388 440 807 808 1455
Cuts at: 6685 618
Size: 1842
Fragments arranged by size:
1842 1455 1388 808 807 627 440 325
217
BanII G_rGCy'C
Cuts at: 1391 2053 2966 3510 3516 5228 1391
Size: 662 913 544 6 1712 4072
Fragments arranged by size:
4072 1712 913 662 544 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2549 4429 4610 928
Size: 971 650 1880 181 4227
Fragments arranged by size:
4227 1880 971 650 181
BbvCI CC'TCA_GC
Cuts at: 2969 2969
Size: 7909
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6100 6134 1903
Size: 34 4163 34 3678
Fragments arranged by size:
4163 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5801 7011 5801
Size: 1210 6699
BclI T'GATC_A
Cuts at: 1548 3144 4180 1548
Size: 1596 1036 5277
Fragments arranged by size:
5277 1596 1036
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2057 6520 136
Size: 107 122 71 631 605 385 4463 1525
Fragments arranged by size:
4463 1525 631 605 385 122 107 71
BglII A'GATC_T
Cuts at: 7904 7904
Size: 7909
BlpI GC'TnA_GC
Cuts at: 5326 5326
Size: 7909
Bme1580I G_kGCm'C
Cuts at: 1790 2224 3516 3916 4425 5970 7126 1790
Size: 434 1292 400 509 1545 1156 2573
Fragments arranged by size:
2573 1545 1292 1156 509 434 400
BmgBI CAC'GTC
Cuts at: 1318 4384 1318
Size: 3066 4843
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3272 4016 5640
Size: 416 53 83 399 24 1855 744 1624
Cuts at: 5640 6598 442
Size: 958 1753
Fragments arranged by size:
1855 1753 1624 958 744 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3245 4097 4617 4927 6569 1239
Size: 2006 852 520 310 1642 2579
Fragments arranged by size:
2579 2006 1642 852 520 310
Bpu10I CC'TnA_GC
Cuts at: 2315 2820 2969 3209 4097 4406 2315
Size: 505 149 240 888 309 5818
Fragments arranged by size:
5818 888 505 309 240 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2432 4958 5108 6034 6899 7197 2423
Size: 9 2526 150 926 865 298 3135
Fragments arranged by size:
3135 2526 926 865 298 150 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2874 3461 3755 6572 882
Size: 617 1375 587 294 2817 2219
Fragments arranged by size:
2817 2219 1375 617 587 294
BsaAI yAC'GTr
Cuts at: 493 1204 5700 493
Size: 711 4496 2702
Fragments arranged by size:
4496 2702 711
BsaBI GATnn'nnATC
Cuts at: 3188 7903 3188
Size: 4715 3194
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4560 5716 6098 6733
Size: 53 83 186 936 3027 1156 382 635
Cuts at: 6733 275
Size: 1451
Fragments arranged by size:
3027 1451 1156 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2124 3186 3324 4900 6342 6740 7014 7161 7572
Size: 1062 138 1576 1442 398 274 147 411
Cuts at: 7572 2124
Size: 2461
Fragments arranged by size:
2461 1576 1442 1062 411 398 274 147
138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2948 2978 4481 4511 4938
Size: 30 165 30 1545 30 1503 30 427
Cuts at: 4938 4968 1178
Size: 30 4119
Fragments arranged by size:
4119 1545 1503 427 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3002 3005 3685 5092 1186
Size: 1816 3 680 1407 4003
Fragments arranged by size:
4003 1816 1407 680 3
BseYI C'CCAG_C
Cuts at: 4110 4237 4348 7112 7608 4110
Size: 127 111 2764 496 4411
Fragments arranged by size:
4411 2764 496 127 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 2715 1163
Size: 573 363 616 6357
Fragments arranged by size:
6357 616 573 363
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2759 5089 5359 6120 7148 1540
Size: 275 186 758 2330 270 761 1028 2301
Fragments arranged by size:
2330 2301 1028 761 758 275 270 186
BsiHKAI G_wGCw'C
Cuts at: 2224 3276 3510 3882 5970 6055 7126 2224
Size: 1052 234 372 2088 85 1071 3007
Fragments arranged by size:
3007 2088 1071 1052 372 234 85
BsmI GAATG_Cn'
Cuts at: 3548 4671 5420 5513 3548
Size: 1123 749 93 5944
Fragments arranged by size:
5944 1123 749 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4775 4775
Size: 7909
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2224 2966 3276 3510 3516 3882
Size: 399 263 171 742 310 234 6 366
Cuts at: 3882 3916 4425 5228 5970 6055 7126 1391
Size: 34 509 803 742 85 1071 2174
Fragments arranged by size:
2174 1071 803 742 742 509 399 366
310 263 234 171 85 34 6
BspEI T'CCGG_A
Cuts at: 2124 3186 3324 7572 2124
Size: 1062 138 4248 2461
Fragments arranged by size:
4248 2461 1062 138
BspHI T'CATG_A
Cuts at: 5798 7528 5798
Size: 1730 6179
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3483 4326 5117 5127 5264 844
Size: 2639 843 791 10 137 3489
Fragments arranged by size:
3489 2639 843 791 137 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2095 3814 4954 5796 1394
Size: 429 272 1719 1140 842 3507
Fragments arranged by size:
3507 1719 1140 842 429 272
BsrDI GCAATG_nn'
Cuts at: 65 1248 6404 6578 65
Size: 1183 5156 174 1396
Fragments arranged by size:
5156 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3822 4062 6553 6740 1700
Size: 110 2012 240 2491 187 2869
Fragments arranged by size:
2869 2491 2012 240 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7909
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7909
BssSI C'ACGA_G
Cuts at: 2061 5969 6981 2061
Size: 3908 1012 2989
Fragments arranged by size:
3908 2989 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7909
BstBI TT'CG_AA
Cuts at: 3976 4663 6713 3976
Size: 687 2050 5172
Fragments arranged by size:
5172 2050 687
BstEII G'GTnAC_C
Cuts at: 1184 3985 1184
Size: 2801 5108
BstXI CCAn_nnnn'nTGG
Cuts at: 4075 4418 4075
Size: 343 7566
BstYI r'GATC_y
Cuts at: 1972 3189 3862 4737 5239 5606 5991 6008 7460
Size: 1217 673 875 502 367 385 17 1452
Cuts at: 7460 7546 7558 7904 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1217 875 673 502 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2343 2343
Size: 7909
BtgI C'CryG_G
Cuts at: 513 1065 2647 3517 3787 3981 4843 513
Size: 552 1582 870 270 194 862 3579
Fragments arranged by size:
3579 1582 870 862 552 270 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2582 487
Size: 683 56 1356 5814
Fragments arranged by size:
5814 1356 683 56
BtsI GCAGTG_nn'
Cuts at: 5425 6219 6239 5425
Size: 794 20 7095
Fragments arranged by size:
7095 794 20
ClaI AT'CG_AT
Cuts at: 1554 2043 2890 1554
Size: 489 847 6573
Fragments arranged by size:
6573 847 489
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7874
DraI TTT'AAA
Cuts at: 2188 4812 5213 2188
Size: 2624 401 4884
Fragments arranged by size:
4884 2624 401
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4559 6916 809
Size: 507 3243 2357 1802
Fragments arranged by size:
3243 2357 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2756 3582 5086
Size: 54 1425 50 275 18 926 826 1504
Cuts at: 5086 5356 6245 7874 8
Size: 270 889 1629 43
Fragments arranged by size:
1629 1504 1425 926 889 826 275 270
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2756 5086 5356 1537
Size: 275 944 2330 270 4090
Fragments arranged by size:
4090 2330 944 275 270
EarI CTCTTCn'nnn_
Cuts at: 1353 3278 3539 3558 3672 3887 4206 4331 5141
Size: 1925 261 19 114 215 319 125 810
Cuts at: 5141 5839 1353
Size: 698 3423
Fragments arranged by size:
3423 1925 810 698 319 261 215 125
114 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4049 6496 6870 7016 387
Size: 1064 138 2460 2447 374 146 1280
Fragments arranged by size:
2460 2447 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3508 3508
Size: 7909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3245 3582 4097 4617 4927 4943 5972
Size: 393 1613 337 515 520 310 16 1029
Cuts at: 5972 6569 7356 7624 1239
Size: 597 787 268 1524
Fragments arranged by size:
1613 1524 1029 787 597 520 515 393
337 310 268 16
EcoO109I rG'GnC_Cy
Cuts at: 2377 3513 2377
Size: 1136 6773
EcoRI G'AATT_C
Cuts at: 3706 5218 5596 3706
Size: 1512 378 6019
Fragments arranged by size:
6019 1512 378
EcoRV GAT'ATC
Cuts at: 2161 2161
Size: 7909
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2090 3553 3595 4222 2090
Size: 1463 42 627 5777
Fragments arranged by size:
5777 1463 627 42
FspI TGC'GCA
Cuts at: 6415 6415
Size: 7909
HaeII r_GCGC'y
Cuts at: 1678 2827 3832 4510 7056 1678
Size: 1149 1005 678 2546 2531
Fragments arranged by size:
2546 2531 1149 1005 678
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 4848 4880 4938
Size: 32 135 32 617 32 2627 32 58
Cuts at: 4938 4970 6550 6582 6624 6656 1373
Size: 32 1580 32 42 32 2626
Fragments arranged by size:
2627 2626 1580 617 135 58 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2147 2643 3361 5253 5499 669
Size: 988 281 209 496 718 1892 246 3079
Fragments arranged by size:
3079 1892 988 718 496 281 246 209
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7909
HpaI GTT'AAC
Cuts at: 2643 5499 2643
Size: 2856 5053
KpnI G_GTAC'C
Cuts at: 5234 5234
Size: 7909
MfeI C'AATT_G
Cuts at: 5508 5508
Size: 7909
MluI A'CGCG_T
Cuts at: 7785 7785
Size: 7909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2120 3090 4914 4989 7023 7207 1305
Size: 815 970 1824 75 2034 184 2007
Fragments arranged by size:
2034 2007 1824 970 815 184 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3584 10
Size: 54 1425 343 1752 4335
Fragments arranged by size:
4335 1752 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2580 2780 3590 4136 5183 5867 6226
Size: 648 1414 200 810 546 1047 684 359
Cuts at: 6226 6385 518
Size: 159 2042
Fragments arranged by size:
2042 1414 1047 810 684 648 546 359
200 159
MspA1I CmG'CkG
Cuts at: 1606 2102 3157 4694 4938 4995 5325 6002 7150
Size: 496 1055 1537 244 57 330 677 1148
Cuts at: 7150 7395 1606
Size: 245 2120
Fragments arranged by size:
2120 1537 1148 1055 677 496 330 245
244 57
NaeI GCC'GGC
Cuts at: 3824 3824
Size: 7909
NcoI C'CATG_G
Cuts at: 513 1065 3981 4843 513
Size: 552 2916 862 3579
Fragments arranged by size:
3579 2916 862 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7909
NgoMIV G'CCGG_C
Cuts at: 3822 3822
Size: 7909
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7909
NotI GC'GGCC_GC
Cuts at: 5086 5356 5086
Size: 270 7639
NspI r_CATG'y
Cuts at: 1132 1229 2854 3944 4043 5267 6812 1132
Size: 97 1625 1090 99 1224 1545 2229
Fragments arranged by size:
2229 1625 1545 1224 1090 99 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7909
PciI A'CATG_T
Cuts at: 3940 6808 3940
Size: 2868 5041
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3112 3489 4651 1108
Size: 2004 377 1162 4366
Fragments arranged by size:
4366 2004 1162 377
PfoI T'CCnGG_A
Cuts at: 4067 4594 4067
Size: 527 7382
PmeI GTTT'AAAC
Cuts at: 5213 5213
Size: 7909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5978 6010 7491 7523 5978
Size: 32 1481 32 6364
Fragments arranged by size:
6364 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2377 2377
Size: 7909
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7909
PsiI TTA'TAA
Cuts at: 5479 5479
Size: 7909
PspOMI G'GGCC_C
Cuts at: 3512 3512
Size: 7909
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3970 4002 3970
Size: 32 7877
PstI C_TGCA'G
Cuts at: 830 2154 2466 2634 3162 3370 3444 3930 4323
Size: 1324 312 168 528 208 74 486 393
Cuts at: 4323 4767 5261 830
Size: 444 494 3478
Fragments arranged by size:
3478 1324 528 494 486 444 393 312
208 168 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7909
PvuII CAG'CTG
Cuts at: 2102 3157 4694 4938 2102
Size: 1055 1537 244 5073
Fragments arranged by size:
5073 1537 1055 244
SacI G_AGCT'C
Cuts at: 3510 3510
Size: 7909
SalI G'TCGA_C
Cuts at: 1936 2145 3359 5251 1936
Size: 209 1214 1892 4594
Fragments arranged by size:
4594 1892 1214 209
SapI GCTCTTCn'nnn_
Cuts at: 3539 3672 3887 4331 5141 3539
Size: 133 215 444 810 6307
Fragments arranged by size:
6307 810 444 215 133
SbfI CC_TGCA'GG
Cuts at: 2466 5261 2466
Size: 2795 5114
ScaI AGT'ACT
Cuts at: 6157 7869 6157
Size: 1712 6197
SfcI C'TryA_G
Cuts at: 826 1045 2150 2462 2630 3158 3366 3440 3926
Size: 219 1105 312 168 528 208 74 486
Cuts at: 3926 4319 4441 4763 5137 5257 6392 7073 7264
Size: 393 122 322 374 120 1135 681 191
Cuts at: 7264 826
Size: 1471
Fragments arranged by size:
1471 1135 1105 681 528 486 393 374
322 312 219 208 191 168 122 120
74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7909
SmaI CCC'GGG
Cuts at: 1872 5236 1872
Size: 3364 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2026 2438 2447 4973 5123 6013
Size: 197 926 83 412 9 2526 150 890
Cuts at: 6013 6914 7176 820
Size: 901 262 1553
Fragments arranged by size:
2526 1553 926 901 890 412 262 197
150 83 9
SnaBI TAC'GTA
Cuts at: 493 5700 493
Size: 5207 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7909
SphI G_CATG'C
Cuts at: 1132 5267 1132
Size: 4135 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5833 5
Size: 47 3279 2502 2081
Fragments arranged by size:
3279 2502 2081 47
StuI AGG'CCT
Cuts at: 1517 3340 1517
Size: 1823 6086
StyI C'CwwG_G
Cuts at: 513 1065 3091 3753 3765 3981 3990 4843 5348
Size: 552 2026 662 12 216 9 853 505
Cuts at: 5348 513
Size: 3074
Fragments arranged by size:
3074 2026 853 662 552 505 216 12
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3035 4306 5936 5953 6106 6291 6736
Size: 1255 1455 1271 1630 17 153 185 445
Cuts at: 6736 325
Size: 1498
Fragments arranged by size:
1630 1498 1455 1271 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3930 6155 7867
Size: 274 80 33 51 1443 1953 2225 1712
Cuts at: 7867 96
Size: 138
Fragments arranged by size:
2225 1953 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2948 2980 3906 3930 3938 3962 4362 4394 7869
Size: 32 926 24 8 24 400 32 3475
Cuts at: 7869 7901 2948
Size: 32 2956
Fragments arranged by size:
3475 2956 926 400 32 32 32 24
24 8
Tth111I GACn'n_nGTC
Cuts at: 1316 2664 4543 1316
Size: 1348 1879 4682
Fragments arranged by size:
4682 1879 1348
XbaI T'CTAG_A
Cuts at: 5245 5245
Size: 7909
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3577 4996 3577
Size: 1419 6490
XhoI C'TCGA_G
Cuts at: 1943 2026 1943
Size: 83 7826
XmaI C'CCGG_G
Cuts at: 1870 5234 1870
Size: 3364 4545
XmnI GAAnn'nnTTC
Cuts at: 2556 4590 6038 2556
Size: 2034 1448 4427
Fragments arranged by size:
4427 2034 1448
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4561 5717 276
Size: 53 83 186 936 3027 1156 2468
Fragments arranged by size:
3027 2468 1156 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I DraIII
EcoNI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI