Restriction map for
pFN21AE4261
(Circular) (Six-base) MAPSORT of: pFN21AE4261.seq Check: 95 from: 1 to: 7876
pFN21AE4261 7876 bp
With 182 enzymes: SgfI *
March 28, 2012 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3436 3436
Size: 7876
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5686 278
Size: 53 83 186 936 4150 2468
Fragments arranged by size:
4150 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3061 5219 1937
Size: 1124 2158 4594
Fragments arranged by size:
4594 2158 1124
Acc65I G'GTAC_C
Cuts at: 3039 3359 5197 3039
Size: 320 1838 5718
Fragments arranged by size:
5718 1838 320
AclI AA'CG_TT
Cuts at: 1563 3290 6003 6376 1563
Size: 1727 2713 373 3063
Fragments arranged by size:
3063 2713 1727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3099 3157 5149 5939 7323 7591 1632
Size: 1467 58 1992 790 1384 268 1917
Fragments arranged by size:
1992 1917 1467 1384 790 268 58
AfeI AGC'GCT
Cuts at: 2298 2298
Size: 7876
AflII C'TTAA_G
Cuts at: 820 1017 2555 820
Size: 197 1538 6141
Fragments arranged by size:
6141 1538 197
AflIII A'CryG_T
Cuts at: 1203 2174 6775 7752 1203
Size: 971 4601 977 1327
Fragments arranged by size:
4601 1327 977 971
AgeI A'CCGG_T
Cuts at: 2990 6707 2990
Size: 3717 4159
AhdI GACnn_n'nnGTC
Cuts at: 6605 6605
Size: 7876
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7876
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2256 2940 1184
Size: 1072 684 6120
Fragments arranged by size:
6120 1072 684
AlwNI CAG_nnn'CTG
Cuts at: 4549 4966 5619 7191 4549
Size: 417 653 1572 5234
Fragments arranged by size:
5234 1572 653 417
ApaLI G'TGCA_C
Cuts at: 5933 7089 5933
Size: 1156 6720
ApoI r'AATT_y
Cuts at: 1493 2949 3102 3756 3848 4271 4914 5185 5411
Size: 1456 153 654 92 423 643 271 226
Cuts at: 5411 5563 7672 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1456 654 643 423 271 226
153 152 92
AseI AT'TA_AT
Cuts at: 160 1032 6430 160
Size: 872 5398 1606
Fragments arranged by size:
5398 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7876
AvaI C'yCGr_G
Cuts at: 1870 1943 5201 1870
Size: 73 3258 4545
Fragments arranged by size:
4545 3258 73
BamHI G'GATC_C
Cuts at: 4303 5206 5573 4303
Size: 903 367 6606
Fragments arranged by size:
6606 903 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3039 3359 3619 5197 6652
Size: 325 217 627 1252 320 260 1578 1455
Cuts at: 6652 618
Size: 1842
Fragments arranged by size:
1842 1578 1455 1252 627 325 320 260
217
BanII G_rGCy'C
Cuts at: 1391 2079 2605 5195 1391
Size: 688 526 2590 4072
Fragments arranged by size:
4072 2590 688 526
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2470 4380 928
Size: 971 571 1910 4424
Fragments arranged by size:
4424 1910 971 571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6067 6101 1903
Size: 34 4130 34 3678
Fragments arranged by size:
4130 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5768 6978 5768
Size: 1210 6666
BclI T'GATC_A
Cuts at: 1548 3343 3424 4063 5149 1548
Size: 1795 81 639 1086 4275
Fragments arranged by size:
4275 1795 1086 639 81
BfrBI ATG'CAT
Cuts at: 2024 2376 2024
Size: 352 7524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6487 136
Size: 107 122 71 631 605 4815 1525
Fragments arranged by size:
4815 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2723 3198 7871 2723
Size: 475 4673 2728
Fragments arranged by size:
4673 2728 475
BlpI GC'TnA_GC
Cuts at: 3236 4414 5293 3236
Size: 1178 879 5819
Fragments arranged by size:
5819 1178 879
Bme1580I G_kGCm'C
Cuts at: 1790 5937 7093 1790
Size: 4147 1156 2573
Fragments arranged by size:
4147 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7876
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3913 4097 5057
Size: 416 53 83 399 24 2496 184 960
Cuts at: 5057 5607 6565 442
Size: 550 958 1753
Fragments arranged by size:
2496 1753 960 958 550 416 399 184
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7876
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2649 2829 6536 1239
Size: 1410 180 3707 2579
Fragments arranged by size:
3707 2579 1410 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3659 4202 6001 6866 7164 3659
Size: 543 1799 865 298 4371
Fragments arranged by size:
4371 1799 865 543 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2336 6539 882
Size: 617 837 4203 2219
Fragments arranged by size:
4203 2219 837 617
BsaAI yAC'GTr
Cuts at: 493 1204 5667 493
Size: 711 4463 2702
Fragments arranged by size:
4463 2702 711
BsaBI GATnn'nnATC
Cuts at: 7870 7870
Size: 7876
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5683 6065 6700 275
Size: 53 83 186 936 4150 382 635 1451
Fragments arranged by size:
4150 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2990 6309 6707 6981 7128 7539 2990
Size: 3319 398 274 147 411 3327
Fragments arranged by size:
3327 3319 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4770 4800 1178
Size: 30 165 30 3367 30 4254
Fragments arranged by size:
4254 3367 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2613 4078 5066 1186
Size: 1427 1465 988 3996
Fragments arranged by size:
3996 1465 1427 988
BseYI C'CCAG_C
Cuts at: 2013 2983 7079 7575 2013
Size: 970 4096 496 2314
Fragments arranged by size:
4096 2314 970 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4674 1163
Size: 573 2938 4365
Fragments arranged by size:
4365 2938 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5326 6087 7115 1540
Size: 275 186 3325 761 1028 2301
Fragments arranged by size:
3325 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5937 6022 7093 5937
Size: 85 1071 6720
Fragments arranged by size:
6720 1071 85
BsmI GAATG_Cn'
Cuts at: 2378 3391 4992 5387 5480 2378
Size: 1013 1601 395 93 4774
Fragments arranged by size:
4774 1601 1013 395 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2605 5195 5937 6022 7093 1391
Size: 399 289 526 2590 742 85 1071 2174
Fragments arranged by size:
2590 2174 1071 742 526 399 289 85
BspEI T'CCGG_A
Cuts at: 7539 7539
Size: 7876
BspHI T'CATG_A
Cuts at: 2206 2845 4287 5765 7495 2206
Size: 639 1442 1478 1730 2587
Fragments arranged by size:
2587 1730 1478 1442 639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2224 3436 5231 844
Size: 1380 1212 1795 3489
Fragments arranged by size:
3489 1795 1380 1212
BsrBI CCG'CTC
Cuts at: 1394 1823 5763 1394
Size: 429 3940 3507
Fragments arranged by size:
3940 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3343 4176 4827 6371 6545 65
Size: 1183 2095 833 651 1544 174 1396
Fragments arranged by size:
2095 1544 1396 1183 833 651 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2990 6520 6707 1700
Size: 110 1180 3530 187 2869
Fragments arranged by size:
3530 2869 1180 187 110
BsrGI T'GTAC_A
Cuts at: 96 2169 2422 3980 96
Size: 2073 253 1558 3992
Fragments arranged by size:
3992 2073 1558 253
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7876
BssSI C'ACGA_G
Cuts at: 5936 6948 5936
Size: 1012 6864
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7876
BstBI TT'CG_AA
Cuts at: 6680 6680
Size: 7876
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7876
BstXI CCAn_nnnn'nTGG
Cuts at: 3155 4397 3155
Size: 1242 6634
BstYI r'GATC_y
Cuts at: 1972 2723 3198 4303 5092 5206 5573 5958 5975
Size: 751 475 1105 789 114 367 385 17
Cuts at: 5975 7427 7513 7525 7871 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1105 789 751 475 385 367
346 114 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4181 4181
Size: 7876
BtgI C'CryG_G
Cuts at: 513 1065 3940 513
Size: 552 2875 4449
Fragments arranged by size:
4449 2875 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7137
Fragments arranged by size:
7137 683 56
BtsI GCAGTG_nn'
Cuts at: 3062 5392 6186 6206 3062
Size: 2330 794 20 4732
Fragments arranged by size:
4732 2330 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7876
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2767 2802 529
Size: 35 2203 35 5603
Fragments arranged by size:
5603 2203 35 35
DraI TTT'AAA
Cuts at: 5180 5180
Size: 7876
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2202 6883 809
Size: 507 886 4681 1802
Fragments arranged by size:
4681 1802 886 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2503 5323 6212
Size: 54 1425 50 275 18 673 2820 889
Cuts at: 6212 7841 8
Size: 1629 43
Fragments arranged by size:
2820 1629 1425 889 673 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5323 1537
Size: 275 3511 4090
Fragments arranged by size:
4090 3511 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5006 5806 1353
Size: 3653 800 3423
Fragments arranged by size:
3653 3423 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6463 6837 6983 387
Size: 1064 138 4874 374 146 1280
Fragments arranged by size:
4874 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2649 2829 3099 3157 5149 5939 6536
Size: 393 1017 180 270 58 1992 790 597
Cuts at: 6536 7323 7591 1239
Size: 787 268 1524
Fragments arranged by size:
1992 1524 1017 790 787 597 393 270
268 180 58
EcoNI CCTnn'n_nnAGG
Cuts at: 2940 3614 2940
Size: 674 7202
EcoRI G'AATT_C
Cuts at: 5185 5563 5185
Size: 378 7498
EcoRV GAT'ATC
Cuts at: 2413 3438 3964 2413
Size: 1025 526 6325
Fragments arranged by size:
6325 1025 526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4510 4510
Size: 7876
FspI TGC'GCA
Cuts at: 4265 6382 4265
Size: 2117 5759
HaeII r_GCGC'y
Cuts at: 1678 2300 7023 1678
Size: 622 4723 2531
Fragments arranged by size:
4723 2531 622
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 3508 3540 4770
Size: 32 135 32 543 32 1361 32 1230
Cuts at: 4770 4802 6517 6549 6591 6623 1373
Size: 32 1715 32 42 32 2626
Fragments arranged by size:
2626 1715 1361 1230 543 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3937 4669 5220 5466 669
Size: 988 281 1999 732 551 246 3079
Fragments arranged by size:
3079 1999 988 732 551 281 246
HindIII A'AGCT_T
Cuts at: 748 3124 748
Size: 2376 5500
HpaI GTT'AAC
Cuts at: 4669 5466 4669
Size: 797 7079
KpnI G_GTAC'C
Cuts at: 3043 3363 5201 3043
Size: 320 1838 5718
Fragments arranged by size:
5718 1838 320
MfeI C'AATT_G
Cuts at: 2507 5475 2507
Size: 2968 4908
MluI A'CGCG_T
Cuts at: 7752 7752
Size: 7876
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3212 3585 3934 4213 6990 7174 1305
Size: 1907 373 349 279 2777 184 2007
Fragments arranged by size:
2777 2007 1907 373 349 279 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2505 10
Size: 54 1425 343 673 5381
Fragments arranged by size:
5381 1425 673 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2179 5834 6193 6352 518
Size: 648 1013 3655 359 159 2042
Fragments arranged by size:
3655 2042 1013 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2551 2983 4664 5292 5969 7117 7362 1606
Size: 945 432 1681 628 677 1148 245 2120
Fragments arranged by size:
2120 1681 1148 945 677 628 432 245
NcoI C'CATG_G
Cuts at: 513 1065 3940 513
Size: 552 2875 4449
Fragments arranged by size:
4449 2875 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7876
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7876
NotI GC'GGCC_GC
Cuts at: 5323 5323
Size: 7876
NsiI A_TGCA'T
Cuts at: 2026 2378 2026
Size: 352 7524
NspI r_CATG'y
Cuts at: 1132 1229 2178 4321 4540 5234 6779 1132
Size: 97 949 2143 219 694 1545 2229
Fragments arranged by size:
2229 2143 1545 949 694 219 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7876
PciI A'CATG_T
Cuts at: 2174 6775 2174
Size: 4601 3275
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7876
PmeI GTTT'AAAC
Cuts at: 5180 5180
Size: 7876
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5945 5977 7458 7490 5945
Size: 32 1481 32 6331
Fragments arranged by size:
6331 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7876
PsiI TTA'TAA
Cuts at: 2099 4425 5446 2099
Size: 2326 1021 4529
Fragments arranged by size:
4529 2326 1021
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4187 4219 4187
Size: 32 7844
PstI C_TGCA'G
Cuts at: 830 5228 830
Size: 4398 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7876
PvuII CAG'CTG
Cuts at: 2551 2983 4664 2551
Size: 432 1681 5763
Fragments arranged by size:
5763 1681 432
SalI G'TCGA_C
Cuts at: 1936 5218 1936
Size: 3282 4594
SbfI CC_TGCA'GG
Cuts at: 5228 5228
Size: 7876
ScaI AGT'ACT
Cuts at: 3633 3654 6124 7836 3633
Size: 21 2470 1712 3673
Fragments arranged by size:
3673 2470 1712 21
SfcI C'TryA_G
Cuts at: 826 1045 3349 5224 6359 7040 7231 826
Size: 219 2304 1875 1135 681 191 1471
Fragments arranged by size:
2304 1875 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7876
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7876
SmaI CCC'GGG
Cuts at: 1872 5203 1872
Size: 3331 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 3674 4217 5980 6881 7143
Size: 197 926 612 1119 543 1763 901 262
Cuts at: 7143 820
Size: 1553
Fragments arranged by size:
1763 1553 1119 926 901 612 543 262
197
SnaBI TAC'GTA
Cuts at: 493 5667 493
Size: 5174 2702
SpeI A'CTAG_T
Cuts at: 152 5023 152
Size: 4871 3005
SphI G_CATG'C
Cuts at: 1132 4540 5234 1132
Size: 3408 694 3774
Fragments arranged by size:
3774 3408 694
SspI AAT'ATT
Cuts at: 5 52 2260 2395 3086 4156 4889 5800 5
Size: 47 2208 135 691 1070 733 911 2081
Fragments arranged by size:
2208 2081 1070 911 733 691 135 47
StuI AGG'CCT
Cuts at: 1517 3920 1517
Size: 2403 5473
StyI C'CwwG_G
Cuts at: 513 1065 2778 2858 3940 4494 5315 513
Size: 552 1713 80 1082 554 821 3074
Fragments arranged by size:
3074 1713 1082 821 554 552 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2036 4046 5903 5920 6073 6258 6703
Size: 1255 456 2010 1857 17 153 185 445
Cuts at: 6703 325
Size: 1498
Fragments arranged by size:
2010 1857 1498 1255 456 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2068 2169 2422
Size: 274 80 33 51 1443 91 101 253
Cuts at: 2422 2614 3631 3652 3865 3980 6122 7834 96
Size: 192 1017 21 213 115 2142 1712 138
Fragments arranged by size:
2142 1712 1443 1017 274 253 213 192
138 115 101 91 80 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7836 7868 7836
Size: 32 7844
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7876
XbaI T'CTAG_A
Cuts at: 5212 5212
Size: 7876
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7876
XmaI C'CCGG_G
Cuts at: 1870 5201 1870
Size: 3331 4545
XmnI GAAnn'nnTTC
Cuts at: 2992 3292 4929 6005 2992
Size: 300 1637 1076 4863
Fragments arranged by size:
4863 1637 1076 300
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5684 276
Size: 53 83 186 936 4150 2468
Fragments arranged by size:
4150 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI