Restriction map for
pFN21AE4259
(Circular) (Six-base) MAPSORT of: pFN21AE4259.seq Check: 5474 from: 1 to: 7819
pFN21AE4259 7819 bp
With 182 enzymes: SgfI *
March 28, 2012 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2159 2159
Size: 7819
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5629 278
Size: 53 83 186 936 4093 2468
Fragments arranged by size:
4093 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2642 5162 1937
Size: 705 2520 4594
Fragments arranged by size:
4594 2520 705
Acc65I G'GTAC_C
Cuts at: 5140 5140
Size: 7819
AclI AA'CG_TT
Cuts at: 1563 5946 6319 1563
Size: 4383 373 3063
Fragments arranged by size:
4383 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 2892 2903 3050 3356 3382 4701 4836
Size: 767 493 11 147 306 26 1319 135
Cuts at: 4836 5882 7266 7534 1632
Size: 1046 1384 268 1917
Fragments arranged by size:
1917 1384 1319 1046 767 493 306 268
147 135 26 11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7622
AflIII A'CryG_T
Cuts at: 1203 3369 3992 6718 7695 1203
Size: 2166 623 2726 977 1327
Fragments arranged by size:
2726 2166 1327 977 623
AgeI A'CCGG_T
Cuts at: 6650 6650
Size: 7819
AhdI GACnn_n'nnGTC
Cuts at: 4950 6548 4950
Size: 1598 6221
AleI CACnn'nnGTG
Cuts at: 1166 4015 1166
Size: 2849 4970
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3448 4651 1184
Size: 2264 1203 4352
Fragments arranged by size:
4352 2264 1203
AlwNI CAG_nnn'CTG
Cuts at: 2677 3092 3824 4942 5562 7134 2677
Size: 415 732 1118 620 1572 3362
Fragments arranged by size:
3362 1572 1118 732 620 415
ApaLI G'TGCA_C
Cuts at: 5876 7032 5876
Size: 1156 6663
ApoI r'AATT_y
Cuts at: 1493 2104 2445 2694 2864 3834 3953 5057 5128
Size: 611 341 249 170 970 119 1104 71
Cuts at: 5128 5354 5506 7615 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1104 970 611 341 249 226
170 152 119 71
AseI AT'TA_AT
Cuts at: 160 1032 2991 6373 160
Size: 872 1959 3382 1606
Fragments arranged by size:
3382 1959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7819
AvaI C'yCGr_G
Cuts at: 1870 1943 2961 5144 1870
Size: 73 1018 2183 4545
Fragments arranged by size:
4545 2183 1018 73
BamHI G'GATC_C
Cuts at: 5149 5516 5149
Size: 367 7452
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 5140 6595 618
Size: 325 217 627 252 3101 1455 1842
Fragments arranged by size:
3101 1842 1455 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2319 3301 5138 1391
Size: 928 982 1837 4072
Fragments arranged by size:
4072 1837 982 928
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3120 928
Size: 971 1221 5627
Fragments arranged by size:
5627 1221 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2411 2445 6010 6044 1903
Size: 34 474 34 3565 34 3678
Fragments arranged by size:
3678 3565 474 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5045 5711 6921 5045
Size: 666 1210 5943
Fragments arranged by size:
5943 1210 666
BclI T'GATC_A
Cuts at: 1548 2584 3475 3576 5002 1548
Size: 1036 891 101 1426 4365
Fragments arranged by size:
4365 1426 1036 891 101
BfrBI ATG'CAT
Cuts at: 2343 2343
Size: 7819
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6430 136
Size: 107 122 71 631 605 4758 1525
Fragments arranged by size:
4758 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2619 3345 3966 7814 2619
Size: 726 621 3848 2624
Fragments arranged by size:
3848 2624 726 621
BlpI GC'TnA_GC
Cuts at: 4764 5236 4764
Size: 472 7347
Bme1580I G_kGCm'C
Cuts at: 1790 2042 5880 7036 1790
Size: 252 3838 1156 2573
Fragments arranged by size:
3838 2573 1156 252
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7819
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4715 5550 6508
Size: 416 53 83 399 24 3298 835 958
Cuts at: 6508 442
Size: 1753
Fragments arranged by size:
3298 1753 958 835 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7819
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3988 3988
Size: 7819
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 5052 6479 1239
Size: 869 2944 1427 2579
Fragments arranged by size:
2944 2579 1427 869
Bpu10I CC'TnA_GC
Cuts at: 2313 2313
Size: 7819
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3920 5944 6809 7107 3920
Size: 2024 865 298 4632
Fragments arranged by size:
4632 2024 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6482 882
Size: 617 4983 2219
Fragments arranged by size:
4983 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5610 493
Size: 711 4406 2702
Fragments arranged by size:
4406 2702 711
BsaBI GATnn'nnATC
Cuts at: 4878 7813 4878
Size: 2935 4884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5626 6008 6643 275
Size: 53 83 186 936 4093 382 635 1451
Fragments arranged by size:
4093 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6252 6650 6924 7071 7482 6252
Size: 398 274 147 411 6589
Fragments arranged by size:
6589 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7594
Fragments arranged by size:
7594 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2513 2949 3977 1186
Size: 1327 436 1028 5028
Fragments arranged by size:
5028 1327 1028 436
BseYI C'CCAG_C
Cuts at: 2050 3435 5101 7022 7518 2050
Size: 1385 1666 1921 496 2351
Fragments arranged by size:
2351 1921 1666 1385 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7246
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5269 6030 7058 1540
Size: 275 186 3268 761 1028 2301
Fragments arranged by size:
3268 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2319 4534 5068 5880 5965 7036 2319
Size: 2215 534 812 85 1071 3102
Fragments arranged by size:
3102 2215 1071 812 534 85
BsmI GAATG_Cn'
Cuts at: 2343 4275 5330 5423 2343
Size: 1932 1055 93 4739
Fragments arranged by size:
4739 1932 1055 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2319 3301 4534 5068 5138 5880
Size: 399 252 277 982 1233 534 70 742
Cuts at: 5880 5965 7036 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1233 1071 982 742 534 399 277
252 85 70
BspEI T'CCGG_A
Cuts at: 7482 7482
Size: 7819
BspHI T'CATG_A
Cuts at: 4498 5708 7438 4498
Size: 1210 1730 4879
Fragments arranged by size:
4879 1730 1210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2159 5174 844
Size: 1315 3015 3489
Fragments arranged by size:
3489 3015 1315
BsrBI CCG'CTC
Cuts at: 1394 1823 2503 5706 1394
Size: 429 680 3203 3507
Fragments arranged by size:
3507 3203 680 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2529 2848 3558 3765 6314 6488 65
Size: 1183 1281 319 710 207 2549 174 1396
Fragments arranged by size:
2549 1396 1281 1183 710 319 207 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4591 6463 6650 1700
Size: 110 2781 1872 187 2869
Fragments arranged by size:
2869 2781 1872 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7819
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7819
BssSI C'ACGA_G
Cuts at: 5879 6891 5879
Size: 1012 6807
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2536 3824 794
Size: 1742 1288 4789
Fragments arranged by size:
4789 1742 1288
BstBI TT'CG_AA
Cuts at: 4339 6623 4339
Size: 2284 5535
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7819
BstXI CCAn_nnnn'nTGG
Cuts at: 2379 4017 2379
Size: 1638 6181
BstYI r'GATC_y
Cuts at: 1972 2619 3345 3966 5149 5516 5901 5918 7370
Size: 647 726 621 1183 367 385 17 1452
Cuts at: 7370 7456 7468 7814 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1183 726 647 621 385 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 2643 2643
Size: 7819
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2067 2771 513
Size: 552 939 39 24 704 5561
Fragments arranged by size:
5561 939 704 552 39 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7080
Fragments arranged by size:
7080 683 56
BtsI GCAGTG_nn'
Cuts at: 2117 3174 3409 4253 5335 6129 6149 2117
Size: 1057 235 844 1082 794 20 3787
Fragments arranged by size:
3787 1082 1057 844 794 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7819
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4240 4275 4831 4866 529
Size: 35 3676 35 556 35 3482
Fragments arranged by size:
3676 3482 556 35 35 35
DraI TTT'AAA
Cuts at: 2700 4566 4891 5123 2700
Size: 1866 325 232 5396
Fragments arranged by size:
5396 1866 325 232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6826 809
Size: 507 5510 1802
Fragments arranged by size:
5510 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5266 6155 7784
Size: 54 1425 50 275 18 3436 889 1629
Cuts at: 7784 8
Size: 43
Fragments arranged by size:
3436 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5266 1537
Size: 275 3454 4090
Fragments arranged by size:
4090 3454 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3401 3645 4812 5005 5749 1353
Size: 2048 244 1167 193 744 3423
Fragments arranged by size:
3423 2048 1167 744 244 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6406 6780 6926 387
Size: 1064 138 4817 374 146 1280
Fragments arranged by size:
4817 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2317 2317
Size: 7819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2399 2892 2903 3050 3356 3382
Size: 393 476 291 493 11 147 306 26
Cuts at: 3382 4701 4836 5052 5882 6479 7266 7534 1239
Size: 1319 135 216 830 597 787 268 1524
Fragments arranged by size:
1524 1319 830 787 597 493 476 393
306 291 268 216 147 135 26 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2060
Size: 7819
EcoO109I rG'GnC_Cy
Cuts at: 2029 2029
Size: 7819
EcoRI G'AATT_C
Cuts at: 2445 3834 3953 5128 5506 2445
Size: 1389 119 1175 378 4758
Fragments arranged by size:
4758 1389 1175 378 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2812 2812
Size: 7819
FspI TGC'GCA
Cuts at: 6325 6325
Size: 7819
HaeII r_GCGC'y
Cuts at: 1678 2077 6966 1678
Size: 399 4889 2531
Fragments arranged by size:
4889 2531 399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3568 3600 4936 4968 6460
Size: 32 135 32 1996 32 1336 32 1492
Cuts at: 6460 6492 6534 6566 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1996 1492 1336 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3998 4480 5163 5409 669
Size: 988 281 2060 482 683 246 3079
Fragments arranged by size:
3079 2060 988 683 482 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7819
HpaI GTT'AAC
Cuts at: 5409 5409
Size: 7819
KpnI G_GTAC'C
Cuts at: 5144 5144
Size: 7819
MfeI C'AATT_G
Cuts at: 3846 4362 5418 3846
Size: 516 1056 6247
Fragments arranged by size:
6247 1056 516
MluI A'CGCG_T
Cuts at: 7695 7695
Size: 7819
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 6933 7117 1305
Size: 813 4815 184 2007
Fragments arranged by size:
4815 2007 813 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5997
Fragments arranged by size:
5997 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3076 4015 4101 4653 5777 6136 6295
Size: 648 1910 939 86 552 1124 359 159
Cuts at: 6295 518
Size: 2042
Fragments arranged by size:
2042 1910 1124 939 648 552 359 159
86
MspA1I CmG'CkG
Cuts at: 1606 2069 2146 2379 5235 5912 7060 7305 1606
Size: 463 77 233 2856 677 1148 245 2120
Fragments arranged by size:
2856 2120 1148 677 463 245 233 77
NaeI GCC'GGC
Cuts at: 4593 4593
Size: 7819
NcoI C'CATG_G
Cuts at: 513 1065 2004 2043 2771 513
Size: 552 939 39 728 5561
Fragments arranged by size:
5561 939 728 552 39
NdeI CA'TA_TG
Cuts at: 387 3306 4630 387
Size: 2919 1324 3576
Fragments arranged by size:
3576 2919 1324
NgoMIV G'CCGG_C
Cuts at: 4591 4591
Size: 7819
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7819
NotI GC'GGCC_GC
Cuts at: 5266 5266
Size: 7819
NsiI A_TGCA'T
Cuts at: 2345 2345
Size: 7819
NspI r_CATG'y
Cuts at: 1132 1229 2343 3373 3947 3996 5177 6722 1132
Size: 97 1114 1030 574 49 1181 1545 2229
Fragments arranged by size:
2229 1545 1181 1114 1030 574 97 49
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7819
PciI A'CATG_T
Cuts at: 3369 3992 6718 3369
Size: 623 2726 4470
Fragments arranged by size:
4470 2726 623
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7819
PmeI GTTT'AAAC
Cuts at: 5123 5123
Size: 7819
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5888 5920 7401 7433 5888
Size: 32 1481 32 6274
Fragments arranged by size:
6274 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7819
PsiI TTA'TAA
Cuts at: 3653 4380 5389 3653
Size: 727 1009 6083
Fragments arranged by size:
6083 1009 727
PspXI vC'TCGA_Gb
Cuts at: 2961 2961
Size: 7819
PstI C_TGCA'G
Cuts at: 830 4248 5171 830
Size: 3418 923 3478
Fragments arranged by size:
3478 3418 923
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7819
PvuII CAG'CTG
Cuts at: 2146 2146
Size: 7819
SacI G_AGCT'C
Cuts at: 2319 2319
Size: 7819
SacII CC_GC'GG
Cuts at: 2070 2070
Size: 7819
SalI G'TCGA_C
Cuts at: 1936 5161 1936
Size: 3225 4594
SapI GCTCTTCn'nnn_
Cuts at: 3645 5005 3645
Size: 1360 6459
SbfI CC_TGCA'GG
Cuts at: 5171 5171
Size: 7819
ScaI AGT'ACT
Cuts at: 6067 7779 6067
Size: 1712 6107
SfcI C'TryA_G
Cuts at: 826 1045 3205 4244 5167 6302 6983 7174 826
Size: 219 2160 1039 923 1135 681 191 1471
Fragments arranged by size:
2160 1471 1135 1039 923 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7819
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7819
SmaI CCC'GGG
Cuts at: 1872 5146 1872
Size: 3274 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2961 3899 5923 6824 7086 820
Size: 197 926 1018 938 2024 901 262 1553
Fragments arranged by size:
2024 1553 1018 938 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5610 493
Size: 5117 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7819
SphI G_CATG'C
Cuts at: 1132 5177 1132
Size: 4045 3774
SspI AAT'ATT
Cuts at: 5 52 2287 4748 5743 5
Size: 47 2235 2461 995 2081
Fragments arranged by size:
2461 2235 2081 995 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7819
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 2771 2850 5258 513
Size: 552 939 39 728 79 2408 3074
Fragments arranged by size:
3074 2408 939 728 552 79 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5846 5863 6016 6201 6646 325
Size: 1255 4266 17 153 185 445 1498
Fragments arranged by size:
4266 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2934 6065 7777
Size: 274 80 33 51 1443 957 3131 1712
Cuts at: 7777 96
Size: 138
Fragments arranged by size:
3131 1712 1443 957 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7779 7811 7779
Size: 32 7787
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7819
XbaI T'CTAG_A
Cuts at: 5155 5155
Size: 7819
XhoI C'TCGA_G
Cuts at: 1943 2961 1943
Size: 1018 6801
XmaI C'CCGG_G
Cuts at: 1870 5144 1870
Size: 3274 4545
XmnI GAAnn'nnTTC
Cuts at: 2736 2877 3330 4047 4413 5948 2736
Size: 141 453 717 366 1535 4607
Fragments arranged by size:
4607 1535 717 453 366 141
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5627 276
Size: 53 83 186 936 4093 2468
Fragments arranged by size:
4093 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI Bsu36I DraIII EcoRV FseI FspAI KasI
NarI NruI PacI PfoI PmlI PpuMI PspOMI PsrI
RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI