Restriction map for
pFN21AE4253
(Circular) (Six-base) MAPSORT of: pFN21AE4253.seq Check: 7651 from: 1 to: 7744
pFN21AE4253 7744 bp
With 182 enzymes: SgfI *
March 28, 2012 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2866 2866
Size: 7744
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5554 278
Size: 53 83 186 936 4018 2468
Fragments arranged by size:
4018 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2104 5087 1937
Size: 167 2983 4594
Fragments arranged by size:
4594 2983 167
Acc65I G'GTAC_C
Cuts at: 2444 5065 2444
Size: 2621 5123
AclI AA'CG_TT
Cuts at: 1563 5871 6244 1563
Size: 4308 373 3063
Fragments arranged by size:
4308 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2199 3399 3655 4014 4054 4625 5807 7191
Size: 567 1200 256 359 40 571 1182 1384
Cuts at: 7191 7459 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1200 1182 571 567 359 268
256 40
AflII C'TTAA_G
Cuts at: 820 1017 2503 820
Size: 197 1486 6061
Fragments arranged by size:
6061 1486 197
AflIII A'CryG_T
Cuts at: 1203 2404 6643 7620 1203
Size: 1201 4239 977 1327
Fragments arranged by size:
4239 1327 1201 977
AgeI A'CCGG_T
Cuts at: 6575 6575
Size: 7744
AhdI GACnn_n'nnGTC
Cuts at: 6473 6473
Size: 7744
AleI CACnn'nnGTG
Cuts at: 1166 2918 1166
Size: 1752 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7744
AlwNI CAG_nnn'CTG
Cuts at: 4415 5487 7059 4415
Size: 1072 1572 5100
Fragments arranged by size:
5100 1572 1072
ApaLI G'TGCA_C
Cuts at: 2264 5801 6957 2264
Size: 3537 1156 3051
Fragments arranged by size:
3537 3051 1156
ApoI r'AATT_y
Cuts at: 1493 2525 2547 2608 2699 3113 3923 4471 4536
Size: 1032 22 61 91 414 810 548 65
Cuts at: 4536 5053 5279 5431 7540 1493
Size: 517 226 152 2109 1697
Fragments arranged by size:
2109 1697 1032 810 548 517 414 226
152 91 65 61 22
AseI AT'TA_AT
Cuts at: 160 1032 3140 3144 6298 160
Size: 872 2108 4 3154 1606
Fragments arranged by size:
3154 2108 1606 872 4
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7744
AvaI C'yCGr_G
Cuts at: 1870 1943 4489 5069 1870
Size: 73 2546 580 4545
Fragments arranged by size:
4545 2546 580 73
AvrII C'CTAG_G
Cuts at: 2095 2095
Size: 7744
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4491 4524 4491
Size: 33 7711
BamHI G'GATC_C
Cuts at: 4875 5074 5441 4875
Size: 199 367 7178
Fragments arranged by size:
7178 367 199
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2444 5065 6520 618
Size: 325 217 627 657 2621 1455 1842
Fragments arranged by size:
2621 1842 1455 657 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2363 2603 3447 5063 1391
Size: 972 240 844 1616 4072
Fragments arranged by size:
4072 1616 972 844 240
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2097 3661 5031 928
Size: 971 198 1564 1370 3641
Fragments arranged by size:
3641 1564 1370 971 198
BbvCI CC'TCA_GC
Cuts at: 3806 3806
Size: 7744
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4573 4607 5935 5969 1903
Size: 34 2636 34 1328 34 3678
Fragments arranged by size:
3678 2636 1328 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2758 2781 5636 6846 2758
Size: 23 2855 1210 3656
Fragments arranged by size:
3656 2855 1210 23
BclI T'GATC_A
Cuts at: 1548 3843 1548
Size: 2295 5449
BfrBI ATG'CAT
Cuts at: 3601 3601
Size: 7744
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3335 4833 6355
Size: 107 122 71 631 605 1663 1498 1522
Cuts at: 6355 136
Size: 1525
Fragments arranged by size:
1663 1525 1522 1498 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3218 3942 7739 3218
Size: 724 3797 3223
Fragments arranged by size:
3797 3223 724
BlpI GC'TnA_GC
Cuts at: 5161 5161
Size: 7744
Bme1580I G_kGCm'C
Cuts at: 1790 2268 3567 4468 4932 5805 6961 1790
Size: 478 1299 901 464 873 1156 2573
Fragments arranged by size:
2573 1299 1156 901 873 478 464
BmgBI CAC'GTC
Cuts at: 1318 2108 2263 1318
Size: 790 155 6799
Fragments arranged by size:
6799 790 155
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3442 4754 5475
Size: 416 53 83 399 24 2025 1312 721
Cuts at: 5475 6433 442
Size: 958 1753
Fragments arranged by size:
2025 1753 1312 958 721 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7744
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4948 4948
Size: 7744
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4372 4461 4488 6404 1239
Size: 3133 89 27 1916 2579
Fragments arranged by size:
3133 2579 1916 89 27
Bpu10I CC'TnA_GC
Cuts at: 3806 3806
Size: 7744
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3629 3719 5869 6734 7032 3629
Size: 90 2150 865 298 4341
Fragments arranged by size:
4341 2150 865 298 90
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3605 4438 4987 6407 882
Size: 617 2106 833 549 1420 2219
Fragments arranged by size:
2219 2106 1420 833 617 549
BsaAI yAC'GTr
Cuts at: 493 1204 4084 5535 493
Size: 711 2880 1451 2702
Fragments arranged by size:
2880 2702 1451 711
BsaBI GATnn'nnATC
Cuts at: 7738 7738
Size: 7744
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3531 5551 5933 6568
Size: 53 83 186 936 1998 2020 382 635
Cuts at: 6568 275
Size: 1451
Fragments arranged by size:
2020 1998 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6177 6575 6849 6996 7407 6177
Size: 398 274 147 411 6514
Fragments arranged by size:
6514 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3434 3464 1178
Size: 30 165 30 2031 30 5458
Fragments arranged by size:
5458 2031 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3338 4467 1186
Size: 2152 1129 4463
Fragments arranged by size:
4463 2152 1129
BseYI C'CCAG_C
Cuts at: 3713 6947 7443 3713
Size: 3234 496 4014
Fragments arranged by size:
4014 3234 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3507 4061 1163
Size: 573 1771 554 4846
Fragments arranged by size:
4846 1771 573 554
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5194 5955 6983 1540
Size: 275 186 3193 761 1028 2301
Fragments arranged by size:
3193 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2268 3685 4058 5805 5890 6961 2268
Size: 1417 373 1747 85 1071 3051
Fragments arranged by size:
3051 1747 1417 1071 373 85
BsmI GAATG_Cn'
Cuts at: 2230 5255 5348 2230
Size: 3025 93 4626
Fragments arranged by size:
4626 3025 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2363 2603 3447 3567 3685 4058
Size: 399 478 95 240 844 120 118 373
Cuts at: 4058 4468 4932 5063 5805 5890 6961 1391
Size: 410 464 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 844 742 478 464 410 399
373 240 131 120 118 95 85
BspEI T'CCGG_A
Cuts at: 7407 7407
Size: 7744
BspHI T'CATG_A
Cuts at: 3472 5633 7363 3472
Size: 2161 1730 3853
Fragments arranged by size:
3853 2161 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2866 3714 5099 844
Size: 2022 848 1385 3489
Fragments arranged by size:
3489 2022 1385 848
BsrBI CCG'CTC
Cuts at: 1394 1823 2973 5631 1394
Size: 429 1150 2658 3507
Fragments arranged by size:
3507 2658 1150 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2677 6239 6413 65
Size: 1183 1429 3562 174 1396
Fragments arranged by size:
3562 1429 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6388 6575 1700
Size: 110 4578 187 2869
Fragments arranged by size:
4578 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4554 96
Size: 4458 3286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7744
BssSI C'ACGA_G
Cuts at: 4577 5804 6816 4577
Size: 1227 1012 5505
Fragments arranged by size:
5505 1227 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7744
BstBI TT'CG_AA
Cuts at: 6548 6548
Size: 7744
BstEII G'GTnAC_C
Cuts at: 1184 3874 1184
Size: 2690 5054
BstXI CCAn_nnnn'nTGG
Cuts at: 2186 2518 4654 2186
Size: 332 2136 5276
Fragments arranged by size:
5276 2136 332
BstYI r'GATC_y
Cuts at: 1972 2135 3218 3420 3490 3942 4320 4875 5074
Size: 163 1083 202 70 452 378 555 199
Cuts at: 5074 5441 5826 5843 7295 7381 7393 7739 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1083 555 452 385 378 367
346 202 199 163 86 70 17 12
Bsu36I CC'TnA_GG
Cuts at: 4923 4923
Size: 7744
BtgI C'CryG_G
Cuts at: 513 1065 2004 2752 3437 3963 4314 513
Size: 552 939 748 685 526 351 3943
Fragments arranged by size:
3943 939 748 685 552 526 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7005
Fragments arranged by size:
7005 683 56
BtsI GCAGTG_nn'
Cuts at: 4353 4857 4873 5260 6054 6074 4353
Size: 504 16 387 794 20 6023
Fragments arranged by size:
6023 794 504 387 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7744
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7709
DraI TTT'AAA
Cuts at: 2706 5048 2706
Size: 2342 5402
DraIII CAC_nnn'GTG
Cuts at: 4084 4084
Size: 7744
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6751 809
Size: 507 5435 1802
Fragments arranged by size:
5435 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 5191 6080
Size: 54 1425 50 275 18 204 3157 889
Cuts at: 6080 7709 8
Size: 1629 43
Fragments arranged by size:
3157 1629 1425 889 275 204 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5191 1537
Size: 275 3379 4090
Fragments arranged by size:
4090 3379 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2218 2351 3045 3713 3990 5674 1353
Size: 865 133 694 668 277 1684 3423
Fragments arranged by size:
3423 1684 865 694 668 277 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3353 4313 6331 6705 6851 387
Size: 1064 138 1764 960 2018 374 146 1280
Fragments arranged by size:
2018 1764 1280 1064 960 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 3399 3655 4014 4054 4372 4461
Size: 393 567 1200 256 359 40 318 89
Cuts at: 4461 4488 4625 5807 6404 7191 7459 1239
Size: 27 137 1182 597 787 268 1524
Fragments arranged by size:
1524 1200 1182 787 597 567 393 359
318 268 256 137 89 40 27
EcoO109I rG'GnC_Cy
Cuts at: 2537 3694 4728 4835 4951 2537
Size: 1157 1034 107 116 5330
Fragments arranged by size:
5330 1157 1034 116 107
EcoRI G'AATT_C
Cuts at: 3113 3923 5053 5431 3113
Size: 810 1130 378 5426
Fragments arranged by size:
5426 1130 810 378
EcoRV GAT'ATC
Cuts at: 2908 2908
Size: 7744
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2706 4630 2518
Size: 188 1924 5632
Fragments arranged by size:
5632 1924 188
FspI TGC'GCA
Cuts at: 6250 6250
Size: 7744
HaeII r_GCGC'y
Cuts at: 1678 4787 6891 1678
Size: 3109 2104 2531
Fragments arranged by size:
3109 2531 2104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2995 3027 3383 3415 3542
Size: 32 135 32 1423 32 356 32 127
Cuts at: 3542 3574 3991 4023 4421 4453 6385 6417 6459
Size: 32 417 32 398 32 1932 32 42
Cuts at: 6459 6491 1373
Size: 32 2626
Fragments arranged by size:
2626 1932 1423 417 398 356 135 127
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5088 5334 669
Size: 988 281 3150 246 3079
Fragments arranged by size:
3150 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3403 748
Size: 2655 5089
HpaI GTT'AAC
Cuts at: 5334 5334
Size: 7744
KpnI G_GTAC'C
Cuts at: 2448 5069 2448
Size: 2621 5123
MfeI C'AATT_G
Cuts at: 4269 5343 4269
Size: 1074 6670
MluI A'CGCG_T
Cuts at: 7620 7620
Size: 7744
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3729 4229 4374 6858 7042 1305
Size: 2424 500 145 2484 184 2007
Fragments arranged by size:
2484 2424 2007 500 184 145
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 10
Size: 54 1425 343 204 5718
Fragments arranged by size:
5718 1425 343 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2918 3774 3968 4016 4652 5702 6061
Size: 648 1752 856 194 48 636 1050 359
Cuts at: 6061 6220 518
Size: 159 2042
Fragments arranged by size:
2042 1752 1050 856 648 636 359 194
159 48
MspA1I CmG'CkG
Cuts at: 1606 3992 4372 4378 4421 5160 5837 6985 7230
Size: 2386 380 6 43 739 677 1148 245
Cuts at: 7230 1606
Size: 2120
Fragments arranged by size:
2386 2120 1148 739 677 380 245 43
6
NcoI C'CATG_G
Cuts at: 513 1065 2004 3963 4314 513
Size: 552 939 1959 351 3943
Fragments arranged by size:
3943 1959 939 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7744
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7744
NotI GC'GGCC_GC
Cuts at: 5191 5191
Size: 7744
NsiI A_TGCA'T
Cuts at: 3603 3603
Size: 7744
NspI r_CATG'y
Cuts at: 1132 1229 3262 3605 5102 6647 1132
Size: 97 2033 343 1497 1545 2229
Fragments arranged by size:
2229 2033 1545 1497 343 97
PacI TTA_AT'TAA
Cuts at: 3144 3144
Size: 7744
PasI CC'CwG_GG
Cuts at: 1348 2541 3495 4522 1348
Size: 1193 954 1027 4570
Fragments arranged by size:
4570 1193 1027 954
PciI A'CATG_T
Cuts at: 6643 6643
Size: 7744
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3068 1108
Size: 1960 5784
PmeI GTTT'AAAC
Cuts at: 5048 5048
Size: 7744
PmlI CAC'GTG
Cuts at: 4084 4084
Size: 7744
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5813 5845 7326 7358 5813
Size: 32 1481 32 6199
Fragments arranged by size:
6199 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4728 4951 4728
Size: 223 7521
PshAI GACnn'nnGTC
Cuts at: 1613 4771 1613
Size: 3158 4586
PsiI TTA'TAA
Cuts at: 5314 5314
Size: 7744
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2267 2299 2267
Size: 32 7712
PstI C_TGCA'G
Cuts at: 830 3461 3737 4360 4377 4420 5096 830
Size: 2631 276 623 17 43 676 3478
Fragments arranged by size:
3478 2631 676 623 276 43 17
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7744
PvuII CAG'CTG
Cuts at: 3992 4372 4378 4421 3992
Size: 380 6 43 7315
Fragments arranged by size:
7315 380 43 6
SalI G'TCGA_C
Cuts at: 1936 5086 1936
Size: 3150 4594
SapI GCTCTTCn'nnn_
Cuts at: 2218 2351 3713 2218
Size: 133 1362 6249
Fragments arranged by size:
6249 1362 133
SbfI CC_TGCA'GG
Cuts at: 5096 5096
Size: 7744
ScaI AGT'ACT
Cuts at: 5992 7704 5992
Size: 1712 6032
SexAI A'CCwGG_T
Cuts at: 4344 4344
Size: 7744
SfcI C'TryA_G
Cuts at: 826 1045 2101 2467 3457 3733 4165 4356 4373
Size: 219 1056 366 990 276 432 191 17
Cuts at: 4373 4416 5092 6227 6908 7099 826
Size: 43 676 1135 681 191 1471
Fragments arranged by size:
1471 1135 1056 990 681 676 432 366
276 219 191 191 43 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7744
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7744
SmaI CCC'GGG
Cuts at: 1872 4491 5071 1872
Size: 2619 580 4545
Fragments arranged by size:
4545 2619 580
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2503 3608 3698 5848 6749 7011
Size: 197 926 560 1105 90 2150 901 262
Cuts at: 7011 820
Size: 1553
Fragments arranged by size:
2150 1553 1105 926 901 560 262 197
90
SnaBI TAC'GTA
Cuts at: 493 5535 493
Size: 5042 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7744
SphI G_CATG'C
Cuts at: 1132 5102 1132
Size: 3970 3774
SspI AAT'ATT
Cuts at: 5 52 2309 3042 5668 5
Size: 47 2257 733 2626 2081
Fragments arranged by size:
2626 2257 2081 733 47
StuI AGG'CCT
Cuts at: 1517 3506 1517
Size: 1989 5755
StyI C'CwwG_G
Cuts at: 513 1065 2004 2095 2812 3963 4314 4452 4623
Size: 552 939 91 717 1151 351 138 171
Cuts at: 4623 4828 4890 5183 513
Size: 205 62 293 3074
Fragments arranged by size:
3074 1151 939 717 552 351 293 205
171 138 91 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2705 3316 4654 5771 5788 5941 6126
Size: 1255 1125 611 1338 1117 17 153 185
Cuts at: 6126 6571 325
Size: 445 1498
Fragments arranged by size:
1498 1338 1255 1125 1117 611 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3930 4554 5990
Size: 274 80 33 51 1443 1953 624 1436
Cuts at: 5990 7702 96
Size: 1712 138
Fragments arranged by size:
1953 1712 1443 1436 624 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3434 3466 4673 4705 7704 7736 3434
Size: 32 1207 32 2999 32 3442
Fragments arranged by size:
3442 2999 1207 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7744
XbaI T'CTAG_A
Cuts at: 5080 5080
Size: 7744
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4312 4312
Size: 7744
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7744
XmaI C'CCGG_G
Cuts at: 1870 4489 5069 1870
Size: 2619 580 4545
Fragments arranged by size:
4545 2619 580
XmnI GAAnn'nnTTC
Cuts at: 2550 5873 2550
Size: 3323 4421
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5552 276
Size: 53 83 186 936 4018 2468
Fragments arranged by size:
4018 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BsiWI BsmBI BstZ17I
EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI PfoI PspOMI PspXI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI