Restriction map for
pFN21AE4237
(Circular) (Six-base) MAPSORT of: pFN21AE4237.seq Check: 4250 from: 1 to: 7846
pFN21AE4237 7846 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5656 278
Size: 53 83 186 936 4120 2468
Fragments arranged by size:
4120 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5123 5189 1937
Size: 3186 66 4594
Fragments arranged by size:
4594 3186 66
Acc65I G'GTAC_C
Cuts at: 5167 5167
Size: 7846
AclI AA'CG_TT
Cuts at: 1563 3469 5973 6346 1563
Size: 1906 2504 373 3063
Fragments arranged by size:
3063 2504 1906 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4575 5909 7293 7561 1632
Size: 2943 1334 1384 268 1917
Fragments arranged by size:
2943 1917 1384 1334 268
AflII C'TTAA_G
Cuts at: 820 1017 3314 820
Size: 197 2297 5352
Fragments arranged by size:
5352 2297 197
AflIII A'CryG_T
Cuts at: 1203 4231 6745 7722 1203
Size: 3028 2514 977 1327
Fragments arranged by size:
3028 2514 1327 977
AgeI A'CCGG_T
Cuts at: 6677 6677
Size: 7846
AhdI GACnn_n'nnGTC
Cuts at: 3108 6575 3108
Size: 3467 4379
AleI CACnn'nnGTG
Cuts at: 1166 3648 1166
Size: 2482 5364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7846
AlwNI CAG_nnn'CTG
Cuts at: 2891 5589 7161 2891
Size: 2698 1572 3576
Fragments arranged by size:
3576 2698 1572
ApaLI G'TGCA_C
Cuts at: 5055 5903 7059 5055
Size: 848 1156 5842
Fragments arranged by size:
5842 1156 848
ApoI r'AATT_y
Cuts at: 1493 2968 3478 4178 4838 5155 5381 5533 7642
Size: 1475 510 700 660 317 226 152 2109
Cuts at: 7642 1493
Size: 1697
Fragments arranged by size:
2109 1697 1475 700 660 510 317 226
152
AseI AT'TA_AT
Cuts at: 160 1032 3560 6400 160
Size: 872 2528 2840 1606
Fragments arranged by size:
2840 2528 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7846
AvaI C'yCGr_G
Cuts at: 1870 1943 2387 2453 5171 1870
Size: 73 444 66 2718 4545
Fragments arranged by size:
4545 2718 444 73 66
AvrII C'CTAG_G
Cuts at: 2218 2218
Size: 7846
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2865 2898 2865
Size: 33 7813
BamHI G'GATC_C
Cuts at: 5176 5543 5176
Size: 367 7479
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5167 6622 618
Size: 325 217 627 3380 1455 1842
Fragments arranged by size:
3380 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2084 4857 5165 1391
Size: 693 2773 308 4072
Fragments arranged by size:
4072 2773 693 308
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2740 3009 928
Size: 971 841 269 5765
Fragments arranged by size:
5765 971 841 269
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6037 6071 1903
Size: 34 4100 34 3678
Fragments arranged by size:
4100 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5738 6948 5738
Size: 1210 6636
BclI T'GATC_A
Cuts at: 1548 4905 1548
Size: 3357 4489
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 6457 136
Size: 107 122 71 631 605 337 4448 1525
Fragments arranged by size:
4448 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3565 7841 3565
Size: 4276 3570
BlpI GC'TnA_GC
Cuts at: 4079 5263 4079
Size: 1184 6662
Bme1580I G_kGCm'C
Cuts at: 1790 2245 5059 5907 7063 1790
Size: 455 2814 848 1156 2573
Fragments arranged by size:
2814 2573 1156 848 455
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7846
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2255 2810 3818
Size: 416 53 83 399 24 838 555 1008
Cuts at: 3818 3905 5577 6535 442
Size: 87 1672 958 1753
Fragments arranged by size:
1753 1672 1008 958 838 555 416 399
87 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 2079 3672 6506 1239
Size: 829 11 1593 2834 2579
Fragments arranged by size:
2834 2579 1593 829 11
Bpu10I CC'TnA_GC
Cuts at: 4848 4848
Size: 7846
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5971 6836 7134 5971
Size: 865 298 6683
Fragments arranged by size:
6683 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2159 6509 882
Size: 617 660 4350 2219
Fragments arranged by size:
4350 2219 660 617
BsaAI yAC'GTr
Cuts at: 493 1204 5068 5637 493
Size: 711 3864 569 2702
Fragments arranged by size:
3864 2702 711 569
BsaBI GATnn'nnATC
Cuts at: 3279 7840 3279
Size: 4561 3285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5653 6035 6670 275
Size: 53 83 186 936 4120 382 635 1451
Fragments arranged by size:
4120 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2168 3174 4945 6279 6677 6951 7098 7509 2168
Size: 1006 1771 1334 398 274 147 411 2505
Fragments arranged by size:
2505 1771 1334 1006 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2515 2545 1178
Size: 30 165 30 1112 30 6479
Fragments arranged by size:
6479 1112 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2076 2513 2520 1186
Size: 859 31 437 7 6512
Fragments arranged by size:
6512 859 437 31 7
BseYI C'CCAG_C
Cuts at: 7049 7545 7049
Size: 496 7350
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4835 1163
Size: 573 3099 4174
Fragments arranged by size:
4174 3099 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 5296 6057 7085 1540
Size: 275 186 15 3280 761 1028 2301
Fragments arranged by size:
3280 2301 1028 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 4135 5059 5907 5992 7063 4135
Size: 924 848 85 1071 4918
Fragments arranged by size:
4918 1071 924 848 85
BsmI GAATG_Cn'
Cuts at: 4960 5357 5450 4960
Size: 397 93 7356
Fragments arranged by size:
7356 397 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2245 4135 4857 5059 5165 5907
Size: 399 294 161 1890 722 202 106 742
Cuts at: 5907 5992 7063 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1890 1071 742 722 399 294 202
161 106 85
BspEI T'CCGG_A
Cuts at: 3174 4945 7509 3174
Size: 1771 2564 3511
Fragments arranged by size:
3511 2564 1771
BspHI T'CATG_A
Cuts at: 3595 5735 7465 3595
Size: 2140 1730 3976
Fragments arranged by size:
3976 2140 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2439 5201 844
Size: 1595 2762 3489
Fragments arranged by size:
3489 2762 1595
BsrBI CCG'CTC
Cuts at: 1394 1823 2528 5733 1394
Size: 429 705 3205 3507
Fragments arranged by size:
3507 3205 705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4994 6341 6515 65
Size: 1183 3746 1347 174 1396
Fragments arranged by size:
3746 1396 1347 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 2312 3243 6490 6677 1700
Size: 110 211 291 931 3247 187 2869
Fragments arranged by size:
3247 2869 931 291 211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7846
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7846
BssSI C'ACGA_G
Cuts at: 5906 6918 5906
Size: 1012 6834
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7846
BstBI TT'CG_AA
Cuts at: 5032 6650 5032
Size: 1618 6228
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7846
BstYI r'GATC_y
Cuts at: 1972 3034 3565 4003 5176 5543 5928 5945 7397
Size: 1062 531 438 1173 367 385 17 1452
Cuts at: 7397 7483 7495 7841 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1173 1062 531 438 385 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 5124 5124
Size: 7846
Bsu36I CC'TnA_GG
Cuts at: 2588 2588
Size: 7846
BtgI C'CryG_G
Cuts at: 513 1065 2121 3061 4739 513
Size: 552 1056 940 1678 3620
Fragments arranged by size:
3620 1678 1056 940 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7107
Fragments arranged by size:
7107 683 56
BtsI GCAGTG_nn'
Cuts at: 2032 2106 4416 5362 6156 6176 2032
Size: 74 2310 946 794 20 3702
Fragments arranged by size:
3702 2310 946 794 74 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7846
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4363 4398 4593 4628 4815 4850 529
Size: 35 3799 35 195 35 187 35 3525
Fragments arranged by size:
3799 3525 195 187 35 35 35 35
DraI TTT'AAA
Cuts at: 3202 4183 4461 5150 3202
Size: 981 278 689 5898
Fragments arranged by size:
5898 981 689 278
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4719 6853 809
Size: 507 3403 2134 1802
Fragments arranged by size:
3403 2134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2310 2511
Size: 54 1425 50 275 18 183 297 201
Cuts at: 2511 4260 5293 6182 7811 8
Size: 1749 1033 889 1629 43
Fragments arranged by size:
1749 1629 1425 1033 889 297 275 201
183 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 5293 1537
Size: 275 201 3280 4090
Fragments arranged by size:
4090 3280 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 2499 3009 3918 4161 5776 1353
Size: 1146 510 909 243 1615 3423
Fragments arranged by size:
3423 1615 1146 909 510 243
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2208 6433 6807 6953 387
Size: 1064 138 619 4225 374 146 1280
Fragments arranged by size:
4225 1280 1064 619 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2079 3672 4575 5909 6506 7293
Size: 393 436 11 1593 903 1334 597 787
Cuts at: 7293 7561 1239
Size: 268 1524
Fragments arranged by size:
1593 1524 1334 903 787 597 436 393
268 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2591 3776 2591
Size: 1185 6661
EcoO109I rG'GnC_Cy
Cuts at: 2646 3135 4116 2646
Size: 489 981 6376
Fragments arranged by size:
6376 981 489
EcoRI G'AATT_C
Cuts at: 5155 5533 5155
Size: 378 7468
EcoRV GAT'ATC
Cuts at: 4012 4012
Size: 7846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3430 3461 3430
Size: 31 7815
FspI TGC'GCA
Cuts at: 6352 6352
Size: 7846
HaeII r_GCGC'y
Cuts at: 1678 6993 1678
Size: 5315 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2515 2547 3094 3126 3700
Size: 32 135 32 943 32 547 32 574
Cuts at: 3700 3732 4754 4786 6487 6519 6561 6593 1373
Size: 32 1022 32 1701 32 42 32 2626
Fragments arranged by size:
2626 1701 1022 943 574 547 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2523 2686 5190 5436 669
Size: 988 281 585 163 2504 246 3079
Fragments arranged by size:
3079 2504 988 585 281 246 163
HindIII A'AGCT_T
Cuts at: 748 3084 4295 5078 748
Size: 2336 1211 783 3516
Fragments arranged by size:
3516 2336 1211 783
HpaI GTT'AAC
Cuts at: 5436 5436
Size: 7846
KpnI G_GTAC'C
Cuts at: 5171 5171
Size: 7846
MfeI C'AATT_G
Cuts at: 5445 5445
Size: 7846
MluI A'CGCG_T
Cuts at: 7722 7722
Size: 7846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2558 3236 3452 6960 7144 1305
Size: 1253 678 216 3508 184 2007
Fragments arranged by size:
3508 2007 1253 678 216 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2513 4262 10
Size: 54 1425 343 681 1749 3594
Fragments arranged by size:
3594 1749 1425 681 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3648 4234 4236 4394 5022 5804 6163
Size: 648 2482 586 2 158 628 782 359
Cuts at: 6163 6322 518
Size: 159 2042
Fragments arranged by size:
2482 2042 782 648 628 586 359 159
158 2
MspA1I CmG'CkG
Cuts at: 1606 2078 2123 5262 5939 7087 7332 1606
Size: 472 45 3139 677 1148 245 2120
Fragments arranged by size:
3139 2120 1148 677 472 245 45
NaeI GCC'GGC
Cuts at: 2023 2023
Size: 7846
NcoI C'CATG_G
Cuts at: 513 1065 3061 513
Size: 552 1996 5298
Fragments arranged by size:
5298 1996 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7846
NgoMIV G'CCGG_C
Cuts at: 2021 2021
Size: 7846
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7846
NotI GC'GGCC_GC
Cuts at: 5293 5293
Size: 7846
NspI r_CATG'y
Cuts at: 1132 1229 4058 4235 5204 6749 1132
Size: 97 2829 177 969 1545 2229
Fragments arranged by size:
2829 2229 1545 969 177 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7846
PciI A'CATG_T
Cuts at: 4231 6745 4231
Size: 2514 5332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7846
PmeI GTTT'AAAC
Cuts at: 5150 5150
Size: 7846
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3336 3368 5915 5947 7428 7460 3336
Size: 32 2547 32 1481 32 3722
Fragments arranged by size:
3722 2547 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2646 4116 2646
Size: 1470 6376
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7846
PsiI TTA'TAA
Cuts at: 2621 3090 3252 3420 5416 2621
Size: 469 162 168 1996 5051
Fragments arranged by size:
5051 1996 469 168 162
PstI C_TGCA'G
Cuts at: 830 3122 3723 5198 830
Size: 2292 601 1475 3478
Fragments arranged by size:
3478 2292 1475 601
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7846
SacII CC_GC'GG
Cuts at: 2124 2124
Size: 7846
SalI G'TCGA_C
Cuts at: 1936 5188 1936
Size: 3252 4594
SanDI GG'GwC_CC
Cuts at: 4116 4116
Size: 7846
SbfI CC_TGCA'GG
Cuts at: 5198 5198
Size: 7846
ScaI AGT'ACT
Cuts at: 3351 6094 7806 3351
Size: 2743 1712 3391
Fragments arranged by size:
3391 2743 1712
SfcI C'TryA_G
Cuts at: 826 1045 3118 3719 4953 5194 6329 7010 7201
Size: 219 2073 601 1234 241 1135 681 191
Cuts at: 7201 826
Size: 1471
Fragments arranged by size:
2073 1471 1234 1135 681 601 241 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7846
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7846
SmaI CCC'GGG
Cuts at: 1872 5173 1872
Size: 3301 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3314 5950 6851 7113 820
Size: 197 926 1371 2636 901 262 1553
Fragments arranged by size:
2636 1553 1371 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5637 493
Size: 5144 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7846
SphI G_CATG'C
Cuts at: 1132 4058 5204 1132
Size: 2926 1146 3774
Fragments arranged by size:
3774 2926 1146
SspI AAT'ATT
Cuts at: 5 52 3190 4581 5770 5
Size: 47 3138 1391 1189 2081
Fragments arranged by size:
3138 2081 1391 1189 47
StuI AGG'CCT
Cuts at: 1517 2991 1517
Size: 1474 6372
StyI C'CwwG_G
Cuts at: 513 1065 2218 3061 5285 513
Size: 552 1153 843 2224 3074
Fragments arranged by size:
3074 2224 1153 843 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2177 4263 4617 5873 5890 6043 6228
Size: 1255 597 2086 354 1256 17 153 185
Cuts at: 6228 6673 325
Size: 445 1498
Fragments arranged by size:
2086 1498 1256 1255 597 445 354 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2653 3349 4170
Size: 274 80 33 51 1443 676 696 821
Cuts at: 4170 6092 7804 96
Size: 1922 1712 138
Fragments arranged by size:
1922 1712 1443 821 696 676 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3156 3188 7806 7838 3156
Size: 32 4618 32 3164
Fragments arranged by size:
4618 3164 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7846
XbaI T'CTAG_A
Cuts at: 4964 5182 4964
Size: 218 7628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2541 2541
Size: 7846
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7846
XmaI C'CCGG_G
Cuts at: 1870 5171 1870
Size: 3301 4545
XmnI GAAnn'nnTTC
Cuts at: 3189 4071 4580 4599 5100 5975 3189
Size: 882 509 19 501 875 5060
Fragments arranged by size:
5060 882 875 509 501 19
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5654 276
Size: 53 83 186 936 4120 2468
Fragments arranged by size:
4120 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI DraIII EcoICRI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI
SfoI SrfI SwaI